期刊
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
卷 43, 期 6, 页码 651-661出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10863-011-9398-8
关键词
Energy metabolism; Reactive oxygen species; Hormesis; Differentiation
资金
- DECIT/SCTIE/MS
- FIOCRUZ
- FAPERJ
- CNPq through the Instituto Nacional de Ciencia e Tecnologia em Biologia Estrutural e Bioimagem
- CAPES/FAPERJ
Trypanosoma cruzi is a hemoflagellate protozoan that causes Chagas' disease. The life cycle of T. cruzi is complex and involves different evolutive forms that have to encounter different environmental conditions provided by the host. Herein, we performed a functional assessment of mitochondrial metabolism in the following two distinct evolutive forms of T. cruzi: the insect stage epimastigote and the freshly isolated bloodstream trypomastigote. We observed that in comparison to epimastigotes, bloodstream trypomastigotes facilitate the entry of electrons into the electron transport chain by increasing complex II-III activity. Interestingly, cytochrome c oxidase (CCO) activity and the expression of CCO subunit IV were reduced in bloodstream forms, creating an electron bottleneck that favored an increase in electron leakage and H2O2 formation. We propose that the oxidative preconditioning provided by this mechanism confers protection to bloodstream trypomastigotes against the host immune system. In this scenario, mitochondrial remodeling during the T. cruzi life cycle may represent a key metabolic adaptation for parasite survival in different hosts.
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