Article
Biochemistry & Molecular Biology
Weifeng Wu, Xiao R. Huang, Yongke You, Liang Xue, Xiao-Jing Wang, Xiaoming Meng, Xiang Lin, Jiangang Shen, Xueqing Yu, Hui-Yao Lan, Haiyong Chen
Summary: The study demonstrates that overexpression of latent TGF-beta 1 can protect against the development of DKD in mice, reducing microalbuminuria and inhibiting renal fibrosis and inflammation, without altering blood glucose levels. This protective effect may be associated with the prevention of Arkadia-mediated Smad7 ubiquitin degradation, suggesting a potential mechanism for the inhibition of DKD by latent TGF-beta 1.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Oncology
Georgia N. N. Delegkou, Maria Birkou, Nefeli Fragkaki, Tamara Toro, Konstantinos D. D. Marousis, Vasso Episkopou, Georgios A. A. Spyroulias
Summary: Ubiquitin-mediated proteasomal degradation is a crucial process in cells that regulates protein concentration. Deregulation of E3 ubiquitin ligases, responsible for substrate recognition, is associated with diseases, especially cancer. Our study provides valuable insights into the interaction and function of Arkadia (RNF111), an E3 ligase, with its physiological E2 partners, UbcH5 Beta, UbcH13, and UbcH7.
Article
Biology
Sadek Amhaz, Batiste Boeda, Mouna Chouchene, Sabrina Colasse, Florent Dingli, Damarys Loew, Julien Henri, Celine Prunier, Laurence Levy
Summary: UBXN7 interacts with the RING domain of RNF111/RNF165 and TOPORS E3 ubiquitin ligases and regulates their activity by inhibiting E2 binding. The UAS thioredoxin-like domain of UBXN7 plays a key role in this regulation. This study reveals a novel mechanism of E3 ubiquitin ligase regulation through specific docking of a thioredoxin-like domain on RING domains.
Article
Microbiology
Om Basukala, Oscar Trejo-Cerro, Michael P. Myers, David Pim, Paola Massimi, Miranda Thomas, Corrado Guarnaccia, David Owen, Lawrence Banks
Summary: HPV-E7 plays a crucial role in HPV-induced malignancy by targeting AP2 complex and modulating clathrin-mediated endocytosis, which may contribute to cellular transformation.
Article
Developmental Biology
Akihito Morita, Yuhkoh Satouh, Hidetaka Kosako, Hisae Kobayashi, Akira Iwase, Ken Sato
Summary: During the oocyte-to-embryo transition and early embryogenesis, PKC-dependent clathrin-mediated endocytosis is essential for the selective degradation of maternal membrane proteins.
Article
Oncology
Sujeong Park, Doyeon Park, Sora Han, Ga Eun Chung, Sujung Soh, Hye In Ka, Hyun Jeong Joo, Young Yang
Summary: This study reveals the crucial role of LY6K in clathrin-and caveolin-1-mediated endocytic pathways regulated by TGF-beta and EGF, and suggests a correlation between LY6K overexpression in cervical cancer cells and poor overall survival.
Article
Biochemical Research Methods
Kyungsoo Kim, Young Sik Kim, Ju Woong Jang, Gyun Min Lee
Summary: In this study, the production of recombinant human transforming growth factor-beta1 (rhTGF-beta 1) in Chinese hamster ovary (CHO) cells was enhanced through characterization and optimization. It was found that mature rhTGF-beta 1 was mostly produced in the form of latent rhTGF-beta 1 in CHO-rhTGF-beta 1 cells, and a significant amount of unprocessed precursors was also produced. Overexpression of PACEsol in CHO-rhTGF-beta 1 cells effectively cleaved the unprocessed precursors, resulting in increased production of mature rhTGF-beta 1.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Ji-chao Wu, Xiao-jie Wang, Jing-han Zhu, Xue-ying Huang, Min Liu, Zhe Qiao, Yan Zhang, Yu Sun, Zi-ying Wang, Peng Zhan, Tao Zhang, Hui-li Hu, Hong Liu, Wei Tang, Fan Yi
Summary: This study found that GPR97 is significantly upregulated in hypertensive nephropathy and is involved in the development of tubulointerstitial fibrosis. GPR97(-/-) mice showed elevated blood pressure but reduced renal injury and fibrosis. Knockdown of GPR97 in cells inhibited TGF-beta signaling by disrupting RhoA-mediated cytoskeletal reorganization and clathrin-mediated endocytosis of TGF-beta receptors.
ACTA PHARMACOLOGICA SINICA
(2023)
Article
Multidisciplinary Sciences
A-Ri Cho, Hee Jung Sul, Yoo Jin Kim, Bohyun Kim, Dae Young Zang
Summary: This study demonstrates that RNF43 frameshift mutations retain normal functionality and have different effects on Wnt/β-catenin signaling. These findings provide important insights for the development of targeted anti-cancer therapies based on the mutation type of RNF43.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Ran Zhao, Yangyang Cao, Yanrui Ge, Jing Xu, Ruofan Li, Mei Yang, Yingying Chen, Dingjie Wu, Jianwei Xiao, Ruili Li
Summary: This study demonstrated that ubiquitination can contribute to the degradation of AMT1;3 proteins under high ammonium stress. Loss of ubiquitination affects the dynamic characteristics and endocytosis of AMT1;3 proteins on the plasma membrane, and inhibits vesicle trafficking pathways under high ammonium conditions. These findings highlight the importance of ubiquitination in the regulation of AMT1;3 proteins.
Review
Medicine, Research & Experimental
Cheng-Yi Huang, Chih-Ling Chung, Tsung-Hui Hu, Jih-Jung Chen, Pei-Feng Liu, Chun-Lin Chen
Summary: TGF-beta plays crucial roles in cancer by promoting proliferation and metastasis, leading pharmaceutical companies to explore inhibitors for cancer therapy. Despite some setbacks and slow progress, research on TGF-beta signal transduction pathway and inhibitors continues to advance in the field of oncology.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biology
Bjorn Schnute, Hideyuki Shimizu, Marvin Lyga, Martin Baron, Thomas Klein
Summary: This study elucidates the important role of lysine-dependent ubiquitylation of the Notch receptor in facilitating the degradation of NICD in the nucleus and preventing the ligand-independent activation of the pathway. It suggests that Notch endocytosis involves several independent mechanisms for its regulation.
Article
Cell Biology
Marisa M. Merino, Carole Seum, Marine Dubois, Marcos Gonzalez-Gaitan
Summary: During organogenesis, morphogen gradients play a crucial role in patterning morphological structures, with gradient scaling ensuring proportional patterns in organs of different sizes. This study reveals a mechanism where cell death trims developing tissue to match the size of the gradient when it is smaller than the organ. The scaling process is regulated by molecular associations involving Dally, Pentagone, and Flower, offering potential insights into the molecular toolbox exploited by tumors.
NATURE CELL BIOLOGY
(2022)
Article
Biochemical Research Methods
Mark Villamil, Weidi Xiao, Clinton Yu, Lan Huang, Ping Xu, Peter Kaiser
Summary: Protein ubiquitylation is an important posttranslational modification that regulates various cellular processes. Different polyubiquitin chain types are recognized and interpreted by different proteins. In this study, the UIML domain of the yeast transcription factor Met4 was identified as a strictly K48-polyubiquitin specific binding unit and used to develop an affinity probe for purification of proteins modified with K48-linked polyubiquitin chains.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Review
Biochemistry & Molecular Biology
Marta Maruszewska-Cheruiyot, Michael James Stear, Maja Machcinska, Katarzyna Donskow-Lysoniewska
Summary: There has been little interaction between parasitologists and oncologists historically, although some helminth infections may predispose to tumor development. Recent research suggests that both tumors and parasites suppress the immune response to increase their chances of survival. They both exploit the TGF beta signaling pathway to modulate the immune response for their own benefit.
