Article
Fisheries
Ji Hun Lee, H. M. V. Udayantha, Qiang Wan, Jehee Lee
Summary: In this study, a TANK ortholog was identified from the red-spotted grouper and found to play a crucial role in immune function, specifically in the antiviral pathway and regulation of NF-kappa B.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Gastroenterology & Hepatology
Camille Martin-Gallausiaux, Diego Garcia-Weber, Amandine Lashermes, Pierre Larraufie, Ludovica Marinelli, Veronica Teixeira, Alice Rolland, Fabienne Beguet-Crespel, Vincent Brochard, Timothe Quatremare, Alexandre Jamet, Joel Dore, Scott D. Gray-Owen, Herve M. Blottiere, Cecile Arrieumerlou, Nicolas Lapaque
Summary: The gut microbiota has an impact on the health of the host through interactions with intestinal epithelial cells. It has been found that the commensal bacterium A. muciniphila promotes intestinal homeostasis by activating the ALPK1/TIFA/TRAF6 axis, a pathway associated with innate immunity.
Article
Virology
Brianna M. Busscher, Henock B. Befekadu, Zhonghua Liu, Tsan Sam Xiao
Summary: This study investigated the roles of the SARS-CoV-2 protein ORF3a in binding to TRAF proteins and activating NF-kappa B. The results suggest that ORF3a can activate NF-kappa B through alternative mechanisms.
Article
Biochemistry & Molecular Biology
Chan Nie, Junwei Hu, Bingjie Wang, Hao Li, Xing Yang, Feng Hong
Summary: Little is known about the combined effect of fluoride (F) and arsenic (As) on bone metabolism. This study aimed to explore their effects on specific proteins and genes related to bone formation. The results showed that F exposure was positively correlated with the protein expression of certain factors, while As exposure was negatively correlated. Furthermore, F and As interaction significantly impacted gene expression in bone cells. These findings provide a scientific basis for understanding the interaction of F and As in bone formation.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2023)
Article
Medicine, Research & Experimental
Jiasheng Chen, Wancong Zhang, Chen Pan, Jufeng Fan, Xiaoping Zhong, Shijie Tang
Summary: This study demonstrates that GLA inhibits cell proliferation and induces apoptosis in melanoma cells via the NF-kappa B/p65 pathway. It also effectively suppresses tumor growth without causing toxicity to major organs.
Article
Immunology
Yu Xiao, Jiaqi Liang, Kenneth W. Witwer, Ying Zhang, Qian Wang, Hang Yin
Summary: In our study, we discovered that extracellular vesicles (EVs) play a crucial role in aging, as they can induce chronic inflammation by activating immune cells. We found that EVs derived from senescent cells contain miR-30b-5p, which downregulates SIRT1 and enhances pro-inflammatory cytokine production. Additionally, we observed increased levels of miR-30b-5p in both the aging animal model and EVs harvested from tissues. EVs carrying pro-inflammatory signals can alter immune cell activity in the aging microenvironment, leading to an increase in inflammation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Xiang Chen, Mingchuan Yu, Wei Xu, Linfeng Zou, Jing Ye, Yu Liu, Yuhong Xiao, Jun Luo
Summary: The study found that rutin can inhibit the AGE-induced inflammatory reaction and prevent ECM degradation by targeting the NF-kappa B/MAPK pathway proteins BCL-2 and TRAF-6, making it a potential molecule in treating OA.
Article
Biochemistry & Molecular Biology
Lei Xu, Yangfan Ye, Zeqiang Tao, Tian Wang, Yutian Wei, Wanzhi Cai, Xin Wan, Pengzhan Zhao, Wei Gu, Bin Gu, Liuchao Zhang, Yufei Tian, Ning Liu, Yiming Tu, Jing Ji
Summary: This study elucidated the role of MLPH in GBM radiation resistance, showing that MLPH promotes radiotherapy resistance by regulating the NF-kappa B pathway. Additionally, O-GlcNAcylation of MLPH protects it from degradation and stabilizes its presence in cells. These findings provide insights into a potential mechanism of GBM radiation resistance and suggest a therapeutic strategy for GBM treatment.
Article
Evolutionary Biology
Michelle M. Leger, Nuria Ros-Rocher, Sebastian R. Najle, Inaki Ruiz-Trillo
Summary: The Rel/NF-kappa B transcription factor family plays diverse roles in immunity, development, and differentiation in animals. New research suggests that these proteins originated earlier than previously thought, in the last common ancestor of animals and fungi. The proteins in the sister group to fungi also contain elements necessary for subcellular regulation of Rel/NF-kappa B, similar to those found in animals.
GENOME BIOLOGY AND EVOLUTION
(2022)
Review
Immunology
Cise Kizilirmak, Marco E. Bianchi, Samuel Zambrano
Summary: The transcription factor family NF-kappa B plays a crucial role in inflammation and immunity. Live cell imaging has revealed the dynamic nature of NF-kappa B activation, including oscillations in its nuclear localization. This interdisciplinary research has provided insights into the heterogeneous response of NF-kappa B in different cell types and its impact on gene expression. Future steps in this field should focus on filling existing knowledge gaps.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yongguang Zhang, Dongya Cui, Miaohui Huang, Yongwei Zheng, Baijiao Zheng, Liling Chen, Qi Chen
Summary: The protein NONO is a multifunctional nuclear protein that regulates transcriptional regulation, mRNA splicing, and DNA repair. This study shows that NONO has a critical role in B-cell development and B-cell activation. Deletion of NONO impairs early B-cell development and B-cell maturation, as well as the activation of pathways involved in B-cell activation, such as ERK, AKT, and NF-kappa B.
Review
Immunology
Zhending Gan, Meiyu Zhang, Donghui Xie, Xiaoyan Wu, Changming Hong, Jian Fu, Lijuan Fan, Shengyi Wang, Sufang Han
Summary: Glycine, acting as an inhibitory neurotransmitter, not only affects the functions of macrophages but also shapes their polarization through cellular signaling pathways and microRNAs. Additionally, glycine has beneficial effects in preventing and treating macrophage-associated diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Hongyu Wang, Hongwei Wang, Yinsen Song, Congyan Liu, Xinling Qian, Dalong Zhang, Xin Jiang, Sisen Zhang
Summary: Sepsis-associated encephalopathy (SAE) is a common and severe complication of sepsis, leading to cognitive dysfunction. This study found that overexpression of Foxc1 inhibited microglial migration and reduced inflammatory response and neuronal apoptosis through regulation of the IκBα/NF-κB pathway, thereby improving cognitive dysfunction during SAE.
MOLECULAR MEDICINE REPORTS
(2022)
Article
Multidisciplinary Sciences
Kelly A. McCorkell, Nipun Jayachandran, Michelle D. Cully, Jacquelyn Freund-Brown, Tiffany Weinkopff, James Monslow, Yinling Hu, Ellen Pure, Bruce D. Freedman, Jorge Alvarez, Michael P. Cancro, Michael J. May
Summary: Global inactivation of IKK-alpha leads to defective lymph node formation and B cell maturation. IKK-alpha in distinct EC-derived compartments is uniquely required to promote B cell homeostasis and LN development, and LEC-intrinsic IKK-alpha is absolutely essential for LN formation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Bin Li, Lisi Zheng, Jiayi Ye, Chenmin Zhang, Jie Zhou, Qiaojuan Huang, Yanhua Guo, Luqin Wang, Peng Yu, Shurong Liu, Qiao Lin, Yuxia Luo, Hui Zhou, Jianhua Yang, Lianghu Qu
Summary: This study identifies previously unknown mechanisms of CREB1 in colorectal cancer cell plasticity, including the regulation of lncRNA CCAT1 and NF-kappa B pathways. Knocking out CREB1 triggers epithelial-mesenchymal transition (EMT), inhibits cell proliferation, and enhances cell motility.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Article
Multidisciplinary Sciences
Arnone Nithichanon, Ploenchan Chetchotisakd, Takayuki Matsumura, Yoshimasa Takahashi, Manabu Ato, Takuro Sakagami, Ganjana Lertmemongkolchai
SCIENTIFIC REPORTS
(2020)
Article
Cell Biology
Alitzel Anzurez, Izumi Naka, Shoji Miki, Kaori Nakayama-Hosoya, Mariko Isshiki, Yusuke Watanabe, Midori Nakamura-Hoshi, Sayuri Seki, Takayuki Matsumura, Tomohiro Takano, Taishi Onodera, Yu Adachi, Saya Moriyama, Kazutaka Terahara, Natsuo Tachikawa, Yoshihiro Yoshimura, Hiroaki Sasaki, Hiroshi Horiuchi, Nobuyuki Miyata, Kazuhito Miyazaki, Michiko Koga, Kazuhiko Ikeuchi, Hiroyuki Nagai, Makoto Saito, Eisuke Adachi, Hiroshi Yotsuyanagi, Satoshi Kutsuna, Akira Kawashima, Yusuke Miyazato, Noriko Kinoshita, Chiyoko Kouno, Kensuke Tanaka, Yoshimasa Takahashi, Tadaki Suzuki, Tetsuro Matano, Jun Ohashi, Ai Kawana-Tachikawa
Summary: The study found a significant association between the HLA-DRB1*09:01 allele and severe COVID-19, with a higher risk compared to preexisting medical conditions such as hypertension, diabetes, and cardiovascular diseases. These results suggest a potential role for HLA in predisposing individuals to severe COVID-19.
Article
Immunology
Saya Moriyama, Yu Adachi, Takashi Sato, Keisuke Tonouchi, Lin Sun, Shuetsu Fukushi, Souichi Yamada, Hitomi Kinoshita, Kiyoko Nojima, Takayuki Kanno, Minoru Tobiume, Keita Ishijima, Yudai Kuroda, Eun-Sil Park, Taishi Onodera, Takayuki Matsumura, Tomohiro Takano, Kazutaka Terahara, Masanori Isogawa, Ayae Nishiyama, Ai Kawana-Tachikawa, Masaharu Shinkai, Natsuo Tachikawa, Shigeki Nakamura, Takahiro Okai, Kazu Okuma, Tetsuro Matano, Tsuguto Fujimoto, Ken Maeda, Makoto Ohnishi, Takaji Wakita, Tadaki Suzuki, Yoshimasa Takahashi
Summary: Antibody titers against SARS-CoV-2 decrease over time, but the neutralizing potency against the original virus and variants actually improves with time. Late convalescent antibodies show increased neutralization potency against variants, suggesting that antibody response maturation enhances cross-neutralizing ability against circulating variants. This indicates that declining antibody titers may not necessarily mean declining protection.
Article
Multidisciplinary Sciences
Shoji Miki, Hiroaki Sasaki, Hiroshi Horiuchi, Nobuyuki Miyata, Yukihiro Yoshimura, Kazuhito Miyazaki, Takayuki Matsumura, Yoshimasa Takahashi, Tadaki Suzuki, Tetsuro Matano, Ai Kawana-Tachikawa, Natsuo Tachikawa
Summary: This study demonstrates that SARS-CoV-2 RNAemia is a potent predictive marker of COVID-19 critical condition and mortality, with an adjusted OR for critical condition as high as 125.71.
Article
Immunology
Xiuyuan Lu, Yuki Hosono, Masamichi Nagae, Shigenari Ishizuka, Eri Ishikawa, Daisuke Motooka, Yuki Ozaki, Nicolas Sax, Yuichi Maeda, Yasuhiro Kato, Takayoshi Morita, Ryo Shinnakasu, Takeshi Inoue, Taishi Onodera, Takayuki Matsumura, Masaharu Shinkai, Takashi Sato, Shota Nakamura, Shunsuke Mori, Teru Kanda, Emi E. Nakayama, Tatsuo Shioda, Tomohiro Kurosaki, Kiyoshi Takeda, Atsushi Kumanogoh, Hisashi Arase, Hironori Nakagami, Kazuo Yamashita, Yoshimasa Takahashi, Sho Yamasaki
Summary: Through single-cell TCR and RNA sequencing, researchers identified public cTfh clonotypes expanded in recovered COVID-19 patients, determined their epitopes in the conserved regions of spike protein, and found that these epitopes may be valuable candidates for booster antigens to enhance adaptive immunity against the virus.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Immunology
Ryutaro Kotaki, Yu Adachi, Saya Moriyama, Taishi Onodera, Shuetsu Fukushi, Takaki Nagakura, Keisuke Tonouchi, Kazutaka Terahara, Lin Sun, Tomohiro Takano, Ayae Nishiyama, Masaharu Shinkai, Kunihiro Oba, Fukumi Nakamura-Uchiyama, Hidefumi Shimizu, Tadaki Suzuki, Takayuki Matsumura, Masanori Isogawa, Yoshimasa Takahashi
Summary: Multiple SARS-CoV-2 variants, particularly Beta and Omicron, have the potential to evade neutralizing antibodies, even in those who have received two doses of the BNT162b2 mRNA vaccine. However, boosting with a third vaccine dose or breakthrough infection can improve the overall breadth of neutralizing antibodies. This study longitudinally profiles the cellular composition of RBD-binding memory B cell subsets and their antibody binding and neutralizing activity after the second dose of mRNA vaccine. It finds that two doses of mRNA vaccine induce an expanded antibody breadth over time, while a subset of resting memory B cells show the ability to produce Beta and Omicron-neutralizing antibodies.
SCIENCE IMMUNOLOGY
(2022)
Article
Virology
Kanako Shinada, Takashi Sato, Saya Moriyama, Yu Adachi, Masahiro Shinoda, Shinichiro Ota, Miwa Morikawa, Masamichi Mineshita, Takayuki Matsumura, Yoshimasa Takahashi, Masaharu Shinkai
Summary: The use of favipiravir in the treatment of COVID-19 accelerates viral clearance, preserves the generation and persistence of neutralizing antibodies, and does not interfere with the maturation of neutralizing potency against both WT SARS-CoV-2 and its variants.
Article
Multidisciplinary Sciences
Kazutaka Terahara, Takashi Sato, Yu Adachi, Keisuke Tonouchi, Taishi Onodera, Saya Moriyama, Lin Sun, Tomohiro Takano, Ayae Nishiyama, Ai Kawana-Tachikawa, Tetsuro Matano, Takayuki Matsumura, Masaharu Shinkai, Masanori Isogawa, Yoshimasa Takahashi
Summary: The determinants of memory T cell longevity following SARS-CoV-2 infection are still unknown. This study found that the half-lives of CD4(+) and CD8(+) T cells were longer than antibody titers. Th17-like subset of CD4(+) T cells showed the longest half-life, indicating its close association with T cell longevity. In contrast, Th2 and Tfh-like T cells were more closely correlated with antibody levels.
Article
Multidisciplinary Sciences
Yukie Kashima, Taketoshi Mizutani, Kaori Nakayama-Hosoya, Saya Moriyama, Takayuki Matsumura, Yoshihiro Yoshimura, Hiroaki Sasaki, Hiroshi Horiuchi, Nobuyuki Miyata, Kazuhito Miyazaki, Natsuo Tachikawa, Yoshimasa Takahashi, Tadaki Suzuki, Sumio Sugano, Tetsuro Matano, Ai Kawana-Tachikawa, Yutaka Suzuki
Summary: Through the analysis of single-cell transcriptome and T cell receptor repertoire, differences in cell proportions and gene expression profiles were found between mild and severe cases of COVID-19, as well as among severe cases. In a severely infected elderly patient, characteristics such as depletion of naive T cells, low T cell receptor repertoire diversity, and aberrant hyperactivation of immune cell subsets were observed. This study provides valuable insights into the diversity of immune landscapes and responses.
SCIENTIFIC REPORTS
(2023)
Article
Immunology
Eita Sasaki, Hideki Asanuma, Haruka Momose, Keiko Furuhata, Takuo Mizukami, Takayuki Matsumura, Yoshimasa Takahashi, Isao Hamaguchi
Summary: The mechanisms of how non-mucosal inoculation of adjuvants stimulates the plasmacytoid dendritic cell (pDC)-dependent immunoglobulin (Ig)A response in the lungs are clarified in this study. The combination of systemic inoculation with type 1 interferon (IFN)-inducing adjuvants and intranasal antigen sensitization protects mice against influenza virus infection. These results provide insights into novel mucosal vaccine strategies using non-mucosal inoculated adjuvants.
MUCOSAL IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Tomohiro Takano, Takashi Sato, Ryutaro Kotaki, Saya Moriyama, Shuetsu Fukushi, Masahiro Shinoda, Kiyomi Kabasawa, Nagashige Shimada, Mio Kousaka, Yu Adachi, Taishi Onodera, Kazutaka Terahara, Masanori Isogawa, Takayuki Matsumura, Masaharu Shinkai, Yoshimasa Takahashi
Summary: Takano et al. demonstrate that a heterologous booster using a SARS-CoV-2 recombinant spike protein vaccine induces a more sustained and broader anti-spike receptor-binding domain antibody response compared to a homologous booster using an mRNA vaccine. The study shows that the S-268019-b spike protein booster generates higher and longer-lasting IgG titers specific to the SARS-CoV-2 spike receptor-binding domain, with the ability to bind to antigenically distinct variants, while the BNT162b2 mRNA homologous booster has a weaker effect. Additionally, the S-268019-b booster enhances the production of RBD-angiotensin-converting enzyme 2 (ACE2) binding inhibitory antibodies, resulting in increased neutralizing activities against Omicron BA.1 and BA.5 variants.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Taishi Onodera, Nicolas Sax, Takashi Sato, Yu Adachi, Ryutaro Kotaki, Takeshi Inoue, Ryo Shinnakasu, Takayuki Nakagawa, Shuetsu Fukushi, Tommy Terooatea, Mai Yoshikawa, Keisuke Tonouchi, Takaki Nagakura, Saya Moriyama, Takayuki Matsumura, Masanori Isogawa, Kazutaka Terahara, Tomohiro Takano, Lin Sun, Ayae Nishiyama, Shinnya Omoto, Masaharu Shinkai, Tomohiro Kurosaki, Kazuo Yamashita, Yoshimasa Takahashi
Summary: By analyzing single Bmem cells and assessing antibody function, we have identified the characteristics of Bmem cells that carry potent neutralizing antibodies in COVID-19 convalescent individuals. These neutralizing antibodies are marked by elevated CD62L expression, distinct epitope preference, and usage of convergent VH genes, which contribute to their neutralizing activities. Despite equivalent receptor binding, there is a correlation between neutralizing antibody titers and the CD62L+ subset. Moreover, the dynamics of the CD62L+ subset differ among patients with varying COVID-19 severities. Our Bmem cell profiling reveals the unique phenotype of the subset harboring potent neutralizing BCRs and enhances our understanding of humoral protection.
Article
Cell Biology
Tomohiro Takano, Miwa Morikawa, Yu Adachi, Kiyomi Kabasawa, Nicolas Sax, Saya Moriyama, Lin Sun, Masanori Isogawa, Ayae Nishiyama, Taishi Onodera, Kazutaka Terahara, Keisuke Tonouchi, Masashi Nishimura, Kentaro Tomii, Kazuo Yamashita, Takayuki Matsumura, Masaharu Shinkai, Yoshimasa Takahashi
Summary: By applying high-dimensional immune profiling, the study identified vaccine-induced immune dynamics that correlate with neutralizing antibody levels, adverse event severity, or both. Natural killer cells, dendritic cell subsets, and NKT-like cells were found to play important roles in these immune dynamics.
CELL REPORTS MEDICINE
(2022)
Article
Parasitology
K. Morokuma, T. Matsumura, A. Yamamoto, A. Sakai, T. Hifumi, M. Ato, M. Takahashi
Summary: The equine Yamakagashi antivenom manufactured in 2000 as an unapproved drug showed strong neutralizing ability against the venom's activity and remained stable and effective after 20 years, passing quality control tests and maintaining potency and safety within standard values.
TROPICAL BIOMEDICINE
(2021)
Article
Immunology
Tomohiro Takano, Takayuki Matsumura, Yu Adachi, Kazutaka Terahara, Saya Moriyama, Taishi Onodera, Ayae Nishiyama, Ai Kawana-Tachikawa, Shoji Miki, Kaori Hosoya-Nakayama, Midori Nakamura-Hoshi, Sayuri Seki, Natsuo Tachikawa, Yukihiro Yoshimura, Nobuyuki Miyata, Hiroshi Horiuchi, Hiroaki Sasaki, Kazuhito Miyazaki, Noriko Kinoshita, Tsutomu Sudo, Yutaro Akiyama, Rubuna Sato, Tadaki Suzuki, Tetsuro Matano, Yoshimasa Takahashi
Summary: An expanded myeloid cell compartment is a key feature of severe COVID-19, with polymorphonuclear (PMN)-MDSCs selectively expanding in survivors of severe cases in Japan, positively correlating with IL-8 levels. This transient expansion of PMN-MDSC subset could serve as a predictor of prognosis in severe COVID-19 cases.
INTERNATIONAL IMMUNOLOGY
(2021)