Hesperetin Inhibit Adipocyte Differentiation and Enhance Bax- and p21-Mediated Adipolysis in Human Mesenchymal Stem Cell Adipogenesis

We aimed to explore the antiadipogenic and adipolysis effect of hesperetin in human mesenchymal stem cells (hMSCs)-induced adipogenesis. IC50 value of hesperetin was higher for hMSCs such as 149.2 +/- 13.2 mol for 24 h and 89.4 +/- 11.4 mol in 48 h, whereas in preadipocytes was 87.6 +/- 9.5 mol and 72.4 +/- 5.6 mol in 24 h and 48 h, respectively. Hesperetin treatment (5, 10, and 20 mol) to adipogenesis-induced hMSCs (Group 1) and preadipocytes (Group 2) resulted in a significantly (p < 0.05) increased lipolysis. The treatment with hesperetin decreased the expression of resistin, adiponectin, aP(2), LPL, PPAR-, and TNF- in Groups 1 and 2, whereas a significant increase was observed in Bcl, Bax, and p21 expression in Group 2 compared to untreated preadipocytes. hMSCs cultured in adipogenic medium along with hesperetin significantly inhibited adipocyte differentiation and increased the proapoptotic gene expression levels in preadipocyte. Our result indicates the antiadipogenic and adipolysis effects of hesperetin. (C) 2014 Wiley Periodicals, Inc.