期刊
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
卷 24, 期 1, 页码 5-24出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/0883911508099367
关键词
3D adipose tissue; cell assembly; blood vessel-like structures; adipose derived stem cells (ADSC); tissue engineering; mimetic blood vessels
资金
- National Natural Science Foundation of China/the Research Grants Council of Hong Kong (NSFC/RGC) [50731160625]
- Foundation of Key Laboratory for Advanced Materials Processing Technology, Ministry of Education of China [2006009]
- National Natural Science Foundation of China [30540060, 30440043]
Using a cell assembly device developed in this laboratory, we coextruded adipose derived stem cells (ADSC) and gelatin/alginate hydrogel to form cubic 3D constructions (10 x 10 x 10 mm(3)) with regular distributed go-through pores. The ADSC grew, proliferated, and differentiated within these constructions. With the addition of basic fibroblast growth factor (bFGF), cells located on the scaffold walls differentiated into endothelial like cells while cells embedded in the hydrogel differentiated into adipose like cells. The integrity of the constructions remained for more than 60 days. This new technology enables us to construct 3D adipose tissue with blood vessel-like structures in vitro which is a significant enhancement in adipose tissue engineering and provides a better biomimic 3D model for studying cell-cell interaction, stem cell differentiation conditions and cell organization mechanisms.
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