Article
Plant Sciences
Adam Janotik, Katerina Dadakova, Jan Lochman, Martina Zapletalova
Summary: The study described the molecular processes involved in BABA-induced resistance in tomatoes by analyzing changes in selected metabolites and transcript data induced by BABA, as well as highlighting the impact of BABA on pathogenesis-related proteins and jasmonic acid signaling in Arabidopsis. Additionally, the research found that amino acids L-Asp and L-Gln significantly reduced resistance levels, with the inhibition of BABA-IR due to the rapid metabolization of L-Asp to L-Gln.
Article
Microbiology
Stephany Angelia Tumewu, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Yuki Ichinose
Summary: In this study, four dCache_1 type MCPs in Pta6605 were characterized, with PscA, PscB, and PscC2 responsible for sensing amino acids. Mutant strains of PscA, PscB, and PscC2 showed reduced chemotaxis to most amino acids, as well as effects on cellular functions and virulence in the host tobacco plant.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Roxana Portieles, Hongli Xu, Qiulin Yue, Lin Zhao, Dening Zhang, Lihua Du, Xiangyou Gao, Jingyao Gao, Nayanci Portal Gonzalez, Ramon Santos Bermudez, Orlando Borras-Hidalgo
Summary: This study demonstrates the use of heat-killed endophytic bacterium Bacillus aryabhattai to induce plant defense against fungal and bacterial pathogens through salicylic and jasmonic acid pathways. The presence of gentisic acid in the HKEB is reported for the first time. These findings suggest that HKEBs could be a valuable tool for managing plant diseases.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Marco Zannotti, Kesava Priyan Ramasamy, Valentina Loggi, Alberto Vassallo, Sandra Pucciarelli, Rita Giovannetti
Summary: This study proposes the degradation treatment of diesel using Antarctic bacteria, which is one of the most concerning environmental problems caused by petroleum and its derivatives contaminating aquatic and underground environments. The bacterial strain Marinomonas sp. ef1, isolated from an Antarctic marine ciliate, showed the potential to degrade hydrocarbons commonly present in diesel oil. The bacteria were able to grow and degrade diesel hydrocarbons, indicating their potential use in bioremediation and transformation of these pollutants.
Article
Microbiology
Ayman Badran, Nerhan A. Eid, Amr R. Hassan, Henda Mahmoudi
Summary: This study evaluated the effect of the interaction between quinoa and endophytic bacterial strains on differential responses under biotic stress. The results showed that quinoa plants inoculated with endophytic bacteria had significantly higher chlorophyll content index and grain yield compared to those without bacterial inoculum.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemical Research Methods
Corrie R. Belanger, Robert E. W. Hancock
Summary: This study presents a protocol for testing physiologically relevant infection conditions in minimal inhibitory concentration (MIC) assays, which can be used to assess the susceptibility of bacterial isolates and novel antimicrobial drugs under conditions more similar to clinical infections. The protocol can be applied to most physiologically relevant liquid medium and aerobic pathogens, taking 3-4 days to complete, and may provide a more accurate assessment of the bactericidal effects of new drugs.
Article
Biotechnology & Applied Microbiology
Pragya Mittal, Rashmi Sipani, Apuratha Pandiyan, Shaheen Sulthana, Anurag K. Sinha, Ashaq Hussain, Malay K. Ray, Theetha L. Pavankumar
Summary: RNase R is an important exoribonuclease involved in RNA degradation and stress response in bacteria. Our study reveals a previously unknown role of RNase R in protecting against DNA damage and oxidative stress.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Editorial Material
Biotechnology & Applied Microbiology
Miguel A. Matilla, Tino Krel
Summary: Bacteria adapt to their natural hosts and environments through multiple sensing strategies, such as chemotaxis. In the context of plant pathology, chemotaxis allows phytopathogenic bacteria to detect plant-derived molecules and initiate the infective process. However, the function of plant pathogen chemoreceptors remains poorly understood. Advancing our knowledge of phytopathogen chemoreceptor function is crucial for developing anti-infective therapies for controlling phytopathogens.
MICROBIAL BIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Svenja Battling, Johannes Pastoors, Alexander Deitert, Tobias Goetzen, Lukas Hartmann, Eliot Schroeder, Stanislav Yordanov, Jochen Buechs
Summary: In this study, the authors used the mu RAMOS technology to investigate the auxotrophies and metabolic requirements of Gluconobacter oxydans and developed a defined minimal medium for cultivation. The results showed that the defined minimal medium had similar performance compared to commonly used media, indicating its potential for improving the production process of 5-ketofructose.
JOURNAL OF BIOLOGICAL ENGINEERING
(2022)
Article
Multidisciplinary Sciences
Macarena Toll-Riera, Miriam Olombrada, Francesc Castro-Giner, Andreas Wagner
Summary: Climate change can cause extreme heat waves exceeding the upper thermal limit of organisms. A study on Antarctic bacteria found that they can rapidly adapt to higher temperatures through genomic changes that mitigate protein misfolding. However, there is a physiological limit beyond which populations cannot survive.
Article
Plant Sciences
Xiaomu Zhang, Philip J. Tubergen, Israel D. K. Agorsor, Pramod Khadka, Connor Tembe, Cynthia Denbow, Eva Collakova, Guillaume Pilot, Cristian H. Danna
Summary: Activation of the FLS2 receptor suppresses both early infection stages' virulence and long-term growth of PstDC3000 by promoting glutamine and serine buildup in the leaf apoplast. MAMP perception in Arabidopsis induces the accumulation of free amino acids in a SA-dependent manner. Glutamine and Serine, when co-infiltrated, suppress virulence gene expression and robust infections in susceptible plants.
Article
Plant Sciences
Joyce Alves Goulart da Silva, Mario Lucio Vilela de Resende, Ingridy Simone Ribeiro, Adriene Ribeiro Lima, Luiz Roberto Marques Albuquerque, Ana Cristina Andrade Monteiro, Matheus Henrique Brito Pereira, Deila Magna dos Santos Botelho
Summary: This study compared the chemical composition and antioxidant activity of leaf extracts from a partially resistant coffee cultivar and a susceptible cultivar to bacterial halo blight. The partially resistant cultivar showed higher content of phenolic compounds and flavonoids, as well as higher antioxidant activity.
Article
Plant Sciences
Bret Cooper, Kimberly B. Campbell, Wesley M. Garrett
Summary: This study investigates the mechanism of hypersensitive resistance in beans against Pseudomonas savastanoi pv. phaseolicola. The results indicate that beans produce salicylic acid (SA) and certain phytoalexins during the hypersensitive resistance process, which work together to restrict bacterial replication. These phytoalexins, including daidzein, genistein, and resveratrol, exhibit antibiotic activity against Pseudomonas savastanoi pv. phaseolicola for the first time.
Article
Biology
Yu-Xuan Li, Sheng-Jie Yue, Yi-Fan Zheng, Peng Huang, Yan-Fang Nie, Xiang-Rui Hao, Hong-Yan Zhang, Wei Wang, Hong-Bo Hu, Xue-Hong Zhang
Summary: This study developed a cost-effective minimal medium for efficient synthesis of phenazine compounds. By optimizing the composition of the medium, the production of phenazine compounds by Pseudomonas chlororaphis significantly increased while the cost decreased.
Article
Biochemistry & Molecular Biology
Tomas Jecmen, Roman Tuzhilkin, Miroslav Sulc
Summary: Residue-specific incorporation of non-canonical amino acids (ncAAs) into proteins can introduce bio-orthogonal functionalities. This study investigated protein expression with three methionine analogs in different strains of E. coli and evaluated the impact of ncAAs on bacterial growth and the expression of specific proteins. The results showed varying levels of ncAA incorporation and protein expression in different bacterial strains, indicating the importance of strain selection and optimization for high ncAA content in protein expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)