Article
Multidisciplinary Sciences
Toshiki Nagakubo, Tatsuya Yamamoto, Shumpei Asamizu, Masanori Toyofuku, Nobuhiko Nomura, Hiroyasu Onaka
Summary: Studies have found that Streptomyces can affect microbial competition by producing Streptomyces phage tail-like particles (SLPs), with SLP expression changing according to the competitor species, showing different responses to fungi and Bacillus subtilis.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Sergii Krysenko, Nicole Okoniewski, Merle Nentwich, Arne Matthews, Moritz Baeuerle, Alina Zinser, Tobias Busche, Andreas Kulik, Stephanie Gursch, Annika Kemeny, Agnieszka Bera, Wolfgang Wohlleben
Summary: Streptomyces coelicolor is a soil bacterium with a complex nitrogen metabolism and multiple pathways for polyamine catabolism. The enzyme GlnA2 plays a crucial role in the survival of S. coelicolor under high polyamine concentrations. Other enzymes, such as GlnA3 and GlnA4, are also involved in polyamine degradation. The findings contribute to our understanding of the survival strategies of S. coelicolor in diverse environments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Yeonbum Kim, Jung-Hye Roe, Joo-Hong Park, Yong-Joon Cho, Kang-Lok Lee
Summary: CatR, a peroxide-sensing transcriptional repressor of Fur family, can de-repress the transcription of the catA gene encoding catalase upon peroxide stress in Streptomyces coelicolor. Transcriptomic analysis identified new members of the CatR regulon and a consensus sequence in the promoter region of catR gene and catA operon for direct regulation. Hydrogen peroxide affects the expression of iron uptake genes through DmdR1 and CatR-regulated VtlA protein may play a role in reducing intracellular iron to prevent oxidative stress.
JOURNAL OF MICROBIOLOGY
(2021)
Article
Genetics & Heredity
Buesra Abanoz-Secgin, Cigdem Otur, Sezer Okay, Aslihan Kurt-Kizildogan
Summary: The SCLAV_3199 gene plays a significant role in regulating iron homeostasis and secondary metabolite biosynthesis in Streptomyces clavuligerus.
Article
Biotechnology & Applied Microbiology
Chun-Yan Lin, Yixian Ru, Yanchao Jin, Qi Lin, Guang-Rong Zhao
Summary: The SLCG_7083 protein in Streptomyces lincolnensis plays a regulatory role in morphological development and glucose utilization by repressing genes involved in sporulation, cell division, and lipid metabolism, and activating genes involved in carbon metabolism.
MICROBIAL CELL FACTORIES
(2023)
Article
Biochemistry & Molecular Biology
Karolina Jaworska, Marta Ludwiczak, Emilia Murawska, Adrianna Raczkowska, Katarzyna Brzostek
Summary: The study revealed an important role of OmpR in regulating the expression of the key iron homeostasis protein Fur in Y. enterocolitica, as it binds directly to the promoters of multiple genes involved in iron transport and under Fur repression. OmpR also affects the expression level of the fur gene through negative regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Pamela J. Urrutia, Daniel A. Borquez
Summary: In this study, a comprehensive bioinformatics analysis was performed on the Oximouse dataset to identify potential cysteine redox switches associated with aging in the mouse brain. Multiple pathways related to aging, such as the ubiquitin-proteasome pathway and one-carbon metabolism, were found to have cysteine oxidation changes. The analysis also revealed an asymmetric distribution of cysteine oxidation changes in synaptic proteins, with postsynaptic proteins showing increased cysteine oxidation and presynaptic proteins showing the opposite trend.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Arash Jamali Langeroudi, Mohammad Sadegh Sabet, Mokhtar Jalali-Javaran, Katayoun Zamani, Tahmineh Lohrasebi, Mohammad Ali Malboobi
Summary: This study reveals that the AtPAP17 gene in Arabidopsis thaliana plays a crucial role in root biomass development and has a significant impact on water and phosphate absorption.
BIOTECHNOLOGY LETTERS
(2023)
Article
Toxicology
Vanessa Staldoni de Oliveira, Allisson Jhonatan Gomes Castro, Patricia Acordi Cesconetto, Ariane Zamoner Pacheco de Souza, Jurandir Joaquim Bernardes Junior, Alex Pires de Oliveira Nuner, Carlos Henrique Lemos Soares, Glen Van Der Kraak, Fatima Regina Mena Barreto Silva
Summary: This study compared the effects of pulp and paper mill effluent and betulin on various aspects of testicular physiology in zebrafish, indicating that the alterations may be due to the actions of betulin. Exposure to both effluent and betulin led to decreased lactate and calcium influx, possibly through the activation of the NCX pump. Additionally, betulin treatment resulted in increased ROS, GSH content, GST activity, and a tendency towards decreased CAT activity in the testes.
TOXICOLOGY IN VITRO
(2021)
Article
Biochemistry & Molecular Biology
Shi-Yong Zhu, Li-Li Liu, Yue-Qiang Huang, Xiao-Wei Li, Milton Talukder, Xue-Yan Dai, Yan-Hua Li, Jin-Long Li
Summary: SEPN1 plays a critical role in normal muscular physiology, and mutations can lead to congenital muscular disorders in humans. Despite differences in structure and function between human and chicken SEPN1, they share some common functional domains.
Article
Biology
Wei Niu, Wenchang Zhou, Shuo Lu, Trung Vu, Vasanthi Jayaraman, Jose D. Faraldo-Gomez, Lei Zheng
Summary: The bacterial CAX homolog YfkE can transport Ca2+ and inorganic phosphate (P-i) together in a 1:1 ratio, exchanging them for H+ and becoming a high-flux antiporter when coupled with P-i. This finding uncovers a co-transport mechanism within the CAX family that indicates this class of proteins contributes to the cellular homeostasis of both Ca2+ and phosphate.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Manuela Mengozzi, Andrew Hesketh, Giselda Bucca, Pietro Ghezzi, Colin P. Smith
MOLECULAR MEDICINE
(2020)
Article
Microbiology
Jean-Philippe Langlois, Guillaume Millette, Isabelle Guay, Alexis Dube-Duquette, Suzanne Chamberland, Pierre-Etienne Jacques, Sebastien Rodrigue, Kamal Bouarab, Eric Marsault, Francois Malouin
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Microbiology
Fabrice Betoudji, Taha Abd El Rahman, Marvin J. Miller, Manuka Ghosh, Mario Jacques, Kamal Bouarab, Francois Malouin
Article
Microbiology
Andy Hesketh, Giselda Bucca, Colin P. Smith, Hee-Jeon Hong
Summary: Dalbavancin, vancomycin, and chlorobiphenyl-vancomycin are glycopeptide antibiotics with similar structures and modes of action, but they induce different gene expression patterns and have diverse impacts on bacterial cell wall biosynthesis and other physiological processes.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Celine Ster, Marianne Allard, Julie Cote-Gravel, Simon Boulanger, Pierre Lacasse, Francois Malouin
Summary: The study developed a vaccine formulation for Staphylococcus aureus mastitis using recombinant proteins and adjuvants, successfully inducing immune responses in cows. Different antigens in the vaccine elicited different immune responses, with one antigen causing a balanced immune response and others causing a predominant Th2 response. Vaccinated cows showed a sustained reduction in milk somatic cell counts, indicating a correlation between somatic cell counts and Staphylococcus aureus counts in milk. Further research is needed to understand how different antigens stimulate varying immune responses using the same adjuvant.
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
S. Bennett, I. Fliss, L. Ben Said, F. Malouin, P. Lacasse
Summary: The study evaluated the reduction of bacterial populations through the application of bacteriocin-based teat formulas. The results showed that nisin and the bacteriocin consortium showed the most promise as a teat disinfectant by reducing staphylococci, streptococci, and total bacteria counts.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Immunology
Louise R. Durrant, Giselda Bucca, Andrew Hesketh, Carla Moller-Levet, Laura Tripkovic, Huihai Wu, Kathryn H. Hart, John C. Mathers, Ruan M. Elliott, Susan A. Lanham-New, Colin P. Smith
Summary: Vitamin D plays a crucial role in maintaining bone health and calcium balance, and also has broad effects on cellular physiology and the immune system. This study examines the differences in gene expression following supplementation with vitamin D-2 and D-3, and suggests the existence of ethnic variations in the response.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Thomas A. Burley, Andrew Hesketh, Giselda Bucca, Emma Kennedy, Eleni E. Ladikou, Benjamin P. Towler, Simon Mitchell, Colin P. Smith, Christopher Fegan, Rosalynd Johnston, Andrea Pepper, Chris Pepper
Summary: The study revealed the role of the FAK signaling pathway in CLL cell migration and tissue invasion by investigating the mechanisms underlying CLL cell migration. Inhibition of FAK effectively reduced CLL cell migration and invasion, suggesting the potential for combining FAK inhibition with current targeted therapies to enhance CLL treatment efficacy.
Article
Materials Science, Multidisciplinary
Grace Cooksley, Marcus K. Dymond, Nicolas A. Stewart, Giselda Bucca, Andrew Hesketh, Joseph Lacey, Yury Gogotsi, Susan Sandeman
Summary: This study investigates the impact of two-dimensional titanium carbide MXene on pathways related to inflammation and epithelial-mesenchymal transition (EMT) using an in vitro LECs model. The results demonstrate that Ti3C2T(x) can decrease the expression of inflammatory cytokines and does not promote EMT, promoting a positive wound healing response.
Editorial Material
Engineering, Biomedical
Shirin Saberianpour, Gianluca Melotto, Rachel Forss, Lucy Redhead, Jacqueline Elsom, Nadia Terrazzini, Susan Sandeman, Dipak Sarker, Giselda Bucca, Andrew Hesketh, Cyril Crua, Matteo Santin
EXPERT REVIEW OF MEDICAL DEVICES
(2023)
Article
Infectious Diseases
Guillaume Millette, Evelyne Lacasse, Renaud Binette, Veronique Belley, Louis-Philippe Chaumont, Celine Ster, Francis Beaudry, Kumaraswamy Boyapelly, Pierre-Luc Boudreault, Francois Malouin, Amira A. Moawad, Hosny El-Adawy, Heinrich Neubauer
Summary: Staphylococcus aureus is a major pathogen causing bovine mastitis, and the development of new antibiotics for veterinary use is important due to its virulence and antibiotic resistance. One such antibiotic, PC206, has been found to have specific narrow-spectrum activity against S. aureus and shows promising results in in vitro and in vivo experiments.
Review
Biochemistry & Molecular Biology
Julien A. Delbrouck, Michael Desgagne, Christian Comeau, Kamal Bouarab, Francois Malouin, Pierre-Luc Boudreault