Article
Multidisciplinary Sciences
Calvin K. Lee, William C. Schmidt, Shanice S. Webster, Jonathan W. Chen, George A. O'Toole, Gerard C. L. Wong
Summary: This study reveals that changes in c-di-GMP levels in bacterial biofilms are not the sole factor determining surface commitment, as oscillations in c-di-GMP levels can propagate across generations. Additionally, the study demonstrates that modulation of c-di-GMP signals and cooperative surface commitment can be influenced by varying the interaction strength between PilO and SadC.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Immunology
Siying Lin, Shuzhen Chen, Li Li, Huiluo Cao, Ting Li, Ming Hu, Lisheng Liao, Lian-Hui Zhang, Zeling Xu
Summary: This study characterized a uropathogenic Pseudomonas aeruginosa strain, PA_HN002, and found that its high intracellular c-di-GMP content contributed to enhanced biofilm formation. Analysis of the c-di-GMP metabolizing enzymes in PA_HN002 revealed multiple mutations in phosphodiesterases (PDEs), which resulted in a shifted expression profile and elevated intracellular c-di-GMP levels, promoting biofilm production.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Microbiology
Amber Grace, Rajnish Sahu, Donald R. R. Owen, Vida A. A. Dennis
Summary: Pseudomonas aeruginosa is a common pathogen that has been identified as a multi-drug resistant strain. PAO1 and PA14, two laboratory strains, have contributed significantly to our understanding of the bacterium. However, there are discrepancies between their behaviors and therapeutic susceptibilities observed in human infections. This review aims to compare the two strains and propose methods to improve their clinical relevance.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Jens Bo Andersen, Kasper Norskov Kragh, Louise Dahl Hultqvist, Morten Rybtke, Martin Nilsson, Tim Holm Jakobsen, Michael Givskov, Tim Tolker-Nielsen
Summary: A decade of research has shown the importance of c-di-GMP in bacteria, with high levels associated with biofilm formation and low levels with planktonic bacteria. Inducing specific phosphodiesterases in P. aeruginosa can lead to biofilm dispersal.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Biotechnology & Applied Microbiology
Yu-Ming Cai, Kai-Wei Yu, Ji-Hong Liu, Zhao Cai, Zun-Hao Zhou, Yang Liu, Tian-Fu Wang, Liang Yang
Summary: The c-di-GMP signaling pathways in P. aeruginosa are highly organized and play important roles in the transition between motile and sessile lifestyles. This study identified a phosphodiesterase, PipA, that regulates bacteriophage production, and uncovered a potential signaling circuit for biofilm treatment.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Alexandra J. J. Lee, Georgia Doing, Samuel L. L. Neff, Taylor Reiter, Deborah A. A. Hogan, Casey S. S. Greene
Summary: This article studies the genomic heterogeneity of Pseudomonas aeruginosa strains, compares the expression patterns of core genes and accessory genes between PAO1 and PA14 strains, and finds differences in the expression patterns of some core genes among different strains.
Article
Microbiology
Divakar Badal, Abhijith Vimal Jayarani, Mohammad Ameen Kollaran, Deep Prakash, P. Monisha, Varsha Singh
Summary: Pseudomonas aeruginosa, a human pathogen, demonstrates swarming behavior driven by ethanol as a signaling molecule under nutrient-limiting conditions. This collective behavior is regulated by specific genes and response regulators, and also involves interactions with other microbial species. Ethanol acts as an important environmental cue for the chemotactic response of P. aeruginosa to induce swarming as a foraging behavior.
Article
Multidisciplinary Sciences
Shanice S. Webster, Calvin K. Lee, William C. Schmidt, Gerard C. L. Wong, George A. O'Toole
Summary: This study reveals the importance of the T4P alignment complex protein PilO in modulating biofilm formation by inhibiting the activity of SadC, leading to decreased biofilm formation and increased motility. The interaction between PilO and SadC fine-tunes the levels of c-di-GMP signal at the individual cell level, providing insights into the mechanisms of surface sensing and response in bacteria.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Sarina Bense, Julius Witte, Matthias Preusse, Michal Koska, Lorena Pezoldt, Astrid Droge, Oliver Hartmann, Mathias Musken, Julia Schulze, Timm Fiebig, Heike Baehre, Sebastian Felgner, Andreas Pich, Susanne Haeussler
Summary: C-di-GMP signaling directly affects bacterial behavior by influencing protein functionality, and it can also have a global impact on gene transcription or translation. This study investigated the effects of changes in intracellular c-di-GMP levels on gene expression and protein production in Pseudomonas aeruginosa, and found that an increase in c-di-GMP levels caused a switch in phenotype before any changes in gene transcription or protein abundance were observed.
MOLECULAR MICROBIOLOGY
(2022)
Article
Microbiology
Amal H. Yahya, Sophie R. Harston, William L. Colton, Matthew T. Cabeen
Summary: Pseudomonas aeruginosa is a common pathogen that infects hospitalized patients and individuals with cystic fibrosis. This species is known for forming biofilms, which provide extra protection to the cells and make infections difficult to treat. The deletion of the gene PA14_16550 reduces biofilm formation, and further investigations have identified other genes that regulate biofilm matrix production.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Julia E. E. Dreifus, Lindsey O'Neal, Holly M. M. Jacobs, Adithya S. S. Subramanian, P. Lynne Howell, Daniel J. J. Wozniak, Matthew R. R. Parsek
Summary: The DGC SiaD regulates the production of cell-associated EPS Psl through posttranslational mechanisms in Pseudomonas aeruginosa. The Sia system is important for producing low levels of c-di-GMP and maintaining cell-associated Psl in planktonic populations.
JOURNAL OF BACTERIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Priyajit Banerjee, Pankaj Kumar Sahoo, Sheenu, Anirban Adhikary, Rohit Ruhal, Deepti Jain
Summary: The c-di-GMP signaling pathway is crucial for biofilm regulation in Pseudomonas aeruginosa, involving enzymes such as DGCs and PDEs, as well as effector proteins sensing perturbations in c-di-GMP levels. Understanding these regulatory mechanisms is vital for uncovering pathogen behavior and identifying novel targets for anti-biofilm interventions.
MOLECULAR ASPECTS OF MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Tian Zhou, Jiahui Huang, Zhiqing Liu, Qiqi Lin, Zeling Xu, Lian-Hui Zhang
Summary: The type VI secretion system (T6SS) is an important translocation apparatus used by Gram-negative bacteria to deliver toxic effectors. In this study, a novel regulator FleS/FleR was identified and the regulatory pathway of H1-T6SS in PAO1 was elucidated, providing important implications for the development of novel anti-infective strategies.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Ying Hu, Jeremy Stephen Webb, Shi-qi An
Summary: The rapid emergence of bacterial resistance to antibiotics poses a significant threat to global healthcare systems. Recent research has focused on targeting regulatory pathways, such as the intracellular molecule c-di-GMP, to identify new effective anti-bacterial agents. Altering the cellular concentrations of this molecule has the potential to reduce biofilm formation or increase susceptibility of bacteria to antibiotics.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Microbiology
Qishun Feng, Jianuan Zhou, Lianhui Zhang, Yang Fu, Liang Yang
Summary: The opportunistic human pathogen Pseudomonas aeruginosa can cause severe infections in immunocompromised people or cystic fibrosis (CF) patients. The switch from acute to chronic infection is regulated by the second messenger c-di-GMP in P. aeruginosa, which is involved in regulating important biological processes in pathogenesis. Understanding the complex c-di-GMP signaling network could be beneficial for developing therapeutic approaches and antibacterial agents to combat the threat from P. aeruginosa.
CRITICAL REVIEWS IN MICROBIOLOGY
(2022)
Article
Microbiology
Yun Luo, Kun Zhao, Amy E. Baker, Sherry L. Kuchma, Kimberly A. Coggan, Matthew C. Wolfgang, Gerard C. L. Wong, George A. O'Toole
Article
Microbiology
Amy E. Baker, Andreas Diepold, Sherry L. Kuchma, Jessie E. Scott, Dae Gon Ha, Giulia Orazi, Judith P. Armitage, George A. O'Toole
JOURNAL OF BACTERIOLOGY
(2016)
Article
Chemistry, Multidisciplinary
Audrey Beaussart, Amy E. Baker, Sherry L. Kuchma, Sofiane El-Kirat-Chatel, George A. O'Toole, Yves F. Dufrene
Article
Microbiology
S. L. Kuchma, A. E. Ballok, J. H. Merritt, J. H. Hammond, W. Lu, J. D. Rabinowitz, George A. O'Toole
JOURNAL OF BACTERIOLOGY
(2010)
Article
Microbiology
S. L. Kuchma, E. F. Griffin, G. A. O'Toole
JOURNAL OF BACTERIOLOGY
(2012)
Article
Ecology
Hadeel K. Musafer, Sherry L. Kuchma, Amanda A. Naimie, Joseph D. Schwartzman, Harith J. Fahad AL-Mathkhury, George A. O'Toole
Article
Multidisciplinary Sciences
Albert Siryaporn, Sherry L. Kuchma, George A. O'Toole, Zemer Gitai
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2014)
Article
Microbiology
Amy E. Baker, Shanice S. Webster, Andreas Diepold, Sherry L. Kuchma, Eric Bordeleau, Judith P. Armitage, George A. O'Toole
JOURNAL OF BACTERIOLOGY
(2019)
Article
Microbiology
Jessie E. Scott, Kewei Li, Laura M. Filkins, Bin Zhu, Sherry L. Kuchma, Joseph D. Schwartzman, George A. O'Toole
JOURNAL OF BACTERIOLOGY
(2019)
Article
Microbiology
Kimberley A. Lewis, Amy E. Baker, Annie Chen, Colleen E. Harty, Sherry L. Kuchma, George A. O'Toole, Deborah A. Hogan
JOURNAL OF BACTERIOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Wajiha Khan, Steve P. Bernier, Sherry L. Kuchma, John H. Hammond, Fariha Hasan, George A. O'Toole
INTERNATIONAL MICROBIOLOGY
(2010)
Article
Microbiology
Judith H. Merritt, Kimberly M. Brothers, Sherry L. Kuchma, George A. O'Toole
JOURNAL OF BACTERIOLOGY
(2007)
Article
Microbiology
Sherry L. Kuchma, Kimberly M. Brothers, Judith H. Merritt, Nicole T. Liberati, Frederick M. Ausubel, George A. O'Toole
JOURNAL OF BACTERIOLOGY
(2007)
Article
Biochemistry & Molecular Biology
Lisa Johns, Andrew Grimson, Sherry L. Kuchma, Carrie Loushin Newman, Philip Anderson
MOLECULAR AND CELLULAR BIOLOGY
(2007)