Article
Multidisciplinary Sciences
Aditya Kumar Pal, Anirban Ghosh
Summary: This study investigates the role of secondary messenger c-di-AMP in drug tolerance, specifically in Mycobacterium smegmatis. The findings highlight the specific molecular mechanism linking elevated c-di-AMP levels with resistance mutagenesis and emphasize the significance of non-homology-based DNA repair. Additionally, the study identifies the unique mutational landscape associated with intracellular c-di-AMP levels and demonstrates the critical role of c-di-AMP in driving the evolution of multi-drug tolerance.
SCIENTIFIC REPORTS
(2022)
Article
Plant Sciences
Han-Yi Fu, Ming-Wei Wang
Summary: The extremophilic red alga Cyanidiococcus yangmingshanensis adapts to a wide range of light conditions by accumulating ascorbic acid and activating the enzyme-based reactive oxygen species scavenging system. The activity of ascorbate peroxidase (APX) is significantly enhanced with increasing light intensity and plays a crucial role in photoacclimation through transcriptional regulation of the chloroplast-targeted APX gene.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Multidisciplinary Sciences
Barbara Tizzano, Tobias K. Dallenga, Christian Utpatel, Jochen Behrends, Susanne Homolka, Thomas A. Kohl, Stefan Niemann
Summary: The study reveals that survival and recovery from oxygen starvation in different strains of Mycobacterium tuberculosis are lineage-dependent, with strains belonging to the Euro-American lineage (L4) demonstrating similar survival and resuscitation characteristics, while strains from other lineages show varied responses to oxygen starvation. The findings suggest that resuscitation after oxygen starvation is not a universal feature of all M. tuberculosis strains.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Catherine Vilcheze, Bo Yan, Rosalyn Casey, Suzie Hingley-Wilson, Laurence Ettwiller, William R. Jacobs
Summary: This study provides detailed insights into the transcriptome changes of Mycobacterium tuberculosis under persisting stresses, highlighting genes and gene cohorts involved in stress response. These findings are valuable for the design of novel drug targets and vaccine development.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Neha Khosla, Seema Madhumal Thayil, Rajinder Kaur, Anup Kumar Kesavan
Summary: This study investigated the structure and function of MSMEG_3955, a homologue of Rv3131 from Mycobacterium tuberculosis, using the non-pathogenic mycobacterial model organism Mycobacterium smegmatis. It was revealed that MSMEG_3955 is a FMN-bound flavoprotein existing as a trimer under in vitro conditions, with NADPH-dependent FMN oxidoreductase activity. These findings suggest potential alternative functions of the protein in bacterial survival and biochemical pathways.
Article
Immunology
Richard Long, Angela Lau, Mary Lou Egedahl, Catherine Paulsen, Courtney Heffernan, Brett Edwards, Ryan Cooper
Summary: The proportion of multidrug-resistant tuberculosis among migrants in Canada has been increasing, with some cases attributed to immigration and others to a higher proportion of cases from high MDR tuberculosis burden countries. Local transmission does not play a significant role in the occurrence of MDR tuberculosis in Canada, highlighting the need for improved tuberculosis control in high MDR tuberculosis burden countries.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Review
Microbiology
Manikuntala Kundu, Joyoti Basu
Summary: Mycobacterium tuberculosis can survive in its host for extended periods, remaining dormant within granulomas. Understanding the mechanisms of entry into and exit from dormancy is crucial for developing new tuberculosis treatment strategies. Transcriptomic and proteomic approaches have revealed key genes and pathways involved in dormancy and reactivation of M. tuberculosis.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Microbiology
Elena G. Salina, Vadim Makarov
Summary: Both latent and active TB infections are caused by a heterogeneous population of mycobacteria, and understanding the mechanisms underlying dormant and resuscitation phases of M. tuberculosis can help control latent infection and prevent transmission.
Article
Biochemistry & Molecular Biology
Lei Wang, An Wang, Qian Fu, Zhongyu Shi, Xiaoying Chen, Yan Wang, Wenhui Xu, Tieshan Wang, Shujing Zhang, Sumin Hu
Summary: This study found the occurrence of ferroptosis in radiation-induced intestinal damage, suggesting its relationship with impairment of intestinal immune function. The ferroptosis inhibitor, Liproxstatin-1, was found to ameliorate radiation-induced ferroptosis and promote the recovery from immune imbalances. These findings provide novel strategies for protection against radiation injury through regulation of the ferroptosis pathway.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Jiyan Qiao, Rui Chen, Mengjie Wang, Ru Bai, Xuejing Cui, Ying Liu, Chongming Wu, Chunying Chen
Summary: The research investigated the toxic effects of different types of M/NPLs on mice, showing that oral administration of these M/NPLs caused gut injuries and decreased expression of tight junction proteins, with toxicity ranking as PS-NH2 > PS-COOH > pristine PS. The study also found that M/NPLs led to gut microbiota dysbiosis, with genera containing opportunistic pathogens and deteriorating intestinal barrier function, highlighting the important indirect toxic effect of gut microbiota dysbiosis in M/NPLs-induced gut barrier dysfunction.
Article
Biology
Masuma Khawary, Roopshali Rakshit, Aayush Bahl, Pallavi Juneja, Sashi Kant, Saurabh Pandey, Deeksha Tripathi
Summary: The Mycobacterium tuberculosis protein Tig (Rv2462c) is found in both the membrane fraction and cytosol, and it plays a role in folding newly synthesized polypeptides. Tig is involved in stress adaptation, including thermal and oxidative shock, survival in macrophages, biofilm formation, and immunological responses. It is important for the virulence of Mycobacterium tuberculosis and could be a potential target for tuberculosis interventions.
Review
Immunology
Paul Stewart, Shivani Patel, Andrew Comer, Shafi Muneer, Uzma Nawaz, Violet Quann, Mira Bansal, Vishwanath Venketaraman
Summary: Historically, research on the immunologic response to M. tb infection has mainly focused on T cells and macrophages, disregarding the role of B cells. However, recent studies have shown that B cells play varying roles during different stages of mycobacterial infection. A better understanding of the role of B cells in defense against TB could contribute to the development of effective vaccines and therapies to reduce the burden of disease.
Article
Immunology
Shweta Sharma, Rupesh Chikhale, Nivedita Shinde, A. M. Khan, Vivek Kumar Gupta
Summary: An in vitro hypoxia dormancy model was established using Mycobacterium smegmatis to study the dormant phenotype of Mycobacterium tuberculosis. Three phytomolecules showed activity against dormant bacilli, and further investigation revealed isocitrate lyase and serine/threonine protein kinase as potential targets. These compounds could potentially lead to new therapeutics or be used as adjuvants to reduce the lengthy anti-TB therapy.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ritesh R. Sevalkar, Joel N. Glasgow, Martin Pettinati, Marcelo A. Marti, Vineel P. Reddy, Swati Basu, Elmira Alipour, Daniel B. Kim-Shapiro, Dario A. Estrin, Jack R. Lancaster, Adrie J. C. Steyn
Summary: This study reveals that Mycobacterium tuberculosis can sense and respond to hydrogen sulfide (H2S) signal via the DosS/T/R system. The binding of H2S to DosS heme iron increases DosS autokinase activity and subsequent phosphorylation of DosR, leading to the modulation of gene expression. These findings demonstrate a novel mechanism for Mtb to sense and respond to H2S and highlight the remarkable plasticity of DosS.
Article
Toxicology
Chang Cao, Liangwei Wang, Jing Zhang, Zhanzhan Liu, Mingming Li, Shangfu Xie, Guang Chen, Xiang Xu
Summary: The neurotoxic effects of methamphetamine (METH) can lead to neuronal damage and substance use disorder. This study found that the protein NLG-1 is involved in METH-induced neurotoxicity and may be a potential target for treatment.
TOXICOLOGY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Rahul Balasaheb Aher, Dhiman Sarkar
Summary: The newly approved drug Pretomanid has shown promising antitubercular activity against both replicating and non-replicating forms of Mycobacterium tuberculosis, based on experimental data of its derivatives.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Chemistry, Inorganic & Nuclear
Aliesha Moudgil, Animesh S. Deval, Mahesh S. Dharne, Dhiman M. Sarkar, Amit S. Choudhari, Bhushan P. Chaudhari
Summary: The study successfully synthesized silver nanoparticles from Eichhornia crassipes, characterized them using various techniques, and investigated their antioxidant, antimicrobial, and anticancer activities, showing potential applications in integrative treatment.
JOURNAL OF CLUSTER SCIENCE
(2021)
Article
Chemistry, Organic
Mubarak H. Shaikh, Dnyaneshwar D. Subhedar, Satish V. Akolkar, Amol A. Nagargoje, Vijay M. Khedkar, Dhiman Sarkar, Bapurao B. Shingate
Summary: A small library of tetrazoloquinoline-based 1,2,3-triazoles has been efficiently prepared through click chemistry approach, and some derivatives show promising antimicrobial and antioxidant activity, indicating their potential for further development as novel therapeutic agents.
POLYCYCLIC AROMATIC COMPOUNDS
(2022)
Correction
Chemistry, Medicinal
Mahesh M. Pisal, Ritesh A. Annadate, Meghana C. Athalye, Deepak Kumar, Subhash P. Chavan, Dhiman Sarkar, Hanumant B. Borate
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Chemistry, Organic
Dnyaneshwar D. Subhedar, Mubarak H. Shaikh, Amol A. Nagargoje, Satish Akolkar, Sujit G. Bhansali, Dhiman Sarkar, Bapurao B. Shingate
Summary: Novel conjugates of 3,5-bis(arylidene)-4-piperidones with amide-linkage were successfully synthesized via a one-pot multicomponent reaction, showing remarkable antitubercular activity against M. tuberculosis. The synthesized compounds have potential for further modifications and development in the treatment of tuberculosis.
POLYCYCLIC AROMATIC COMPOUNDS
(2022)
Article
Chemistry, Organic
Shivaji M. Jagadale, Yogita K. Abhale, Hari R. Pawar, Abhijit Shinde, Vivek D. Bobade, Abhijit P. Chavan, Dhiman Sarkar, Pravin C. Mhaske
Summary: A new series of thiazolyl-pyrazolyl-1,2,3-triazole and bis-pyrazolyl-1,2,3-triazole derivatives were synthesized and screened for their antimycobacterial and antibacterial activities. The results showed that these compounds exhibited good inhibitory activity against M. tuberculosis and pathogenic bacteria, suggesting their potential as new compounds for bacterial infection treatment.
POLYCYCLIC AROMATIC COMPOUNDS
(2022)
Article
Chemistry, Applied
Varsha S. Honmore, Arun D. Natu, Vijay M. Khedkar, Manisha A. Arkile, Dhiman Sarkar, Supada R. Rojatkar
Summary: Bioassay-guided isolation of two spiro compounds (1 and 2) from the roots of Artemisia pallens Wall showed promising antibacterial activity, especially against gram negative bacteria, while being inactive against Mycobacterium tuberculosis. Molecular docking study may provide valuable insights into the mechanism of action of these compounds.
NATURAL PRODUCT RESEARCH
(2022)
Article
Chemistry, Medicinal
Nisheeth C. Desai, Kandarp Bhatt, Dharmpalsinh J. Jadeja, Harsh K. Mehta, Vijay M. Khedkar, Dhiman Sarkar
Summary: The study focused on developing a series of new antitubercular drugs utilizing microwave-assisted organic synthesis, highlighting compound 5b as the most effective against Mycobacterium tuberculosis. In silico binding affinity study of mycobacterial enoyl-acyl carrier protein reductase (InhA) revealed the mechanisms and interactions determining the molecule's binding affinity.
DRUG DEVELOPMENT RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Rahul Balasaheb Aher, Dhiman Sarkar
Summary: The study aimed to develop quantitative structure-activity relationship models for the dormant form of Mycobacterium tuberculosis (MTB) and used classifier models to classify active compounds against the dormant MTB, achieving good prediction results.
MOLECULAR DIVERSITY
(2022)
Article
Biochemistry & Molecular Biology
Yogita K. Abhale, Abhijit Shinde, Monika Shelke, Laxman Nawale, Dhiman Sarkar, Pravin C. Mhaske
Summary: A new series of 3-substituted phenyl-2-(2-(substituted phenyl)thiazol-4-yl) thiazolidin-4-one derivatives were synthesized and screened for antimycobacterial activity, with nine derivatives showing excellent activity against Mycobacterium bovis BCG. The compounds also exhibited low cytotoxicity against various cell lines, suggesting their potential for treating tuberculosis.
BIOORGANIC CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Sampa Sarkar, Sagar Swami, Sarvesh Kumar Soni, Jessica K. Holien, Arshad Khan, Arvind M. Korwar, Anjali P. Likhite, Ramesh A. Joshi, Rohini R. Joshi, Dhiman Sarkar
Summary: A potent lead compound RRA2 was identified in this study, showing significant mycobactericidal activity against active stage Mycobacterium bovis BCG and Mtb with high selectivity index. Using click chemistry, a biotin linker-RRA2 conjugate was synthesized to interact with a specific target protein GroEl2, confirmed by HR-MS analysis.
MOLECULAR DIVERSITY
(2022)
Article
Chemistry, Organic
Dnyaneshwar D. Subhedar, Mubarak H. Shaikh, Amol A. Nagargoje, Dhiman Sarkar, Vijay M. Khedkar, Bapurao B. Shingate
Summary: A series of new benzimidazole-incorporated compounds were synthesized and showed promising antimycobacterial activity without cytotoxicity, making them potential drug leads for TB treatment.
POLYCYCLIC AROMATIC COMPOUNDS
(2022)
Article
Multidisciplinary Sciences
Sonia Agrawal, Suwarna Gample, Amar Yeware, Dhiman Sarkar
Summary: The study found that nitrite can induce dormancy in Mycobacterium tuberculosis by increasing the expression of the MRA2164 gene and generating nitric oxide, which plays a crucial role in maintaining the non-replicative stage of the bacilli.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Mark L. Sowers, Hui Tang, Vipul K. Singh, Arshad Khan, Abhishek Mishra, Blanca I. Restrepo, Chinnaswamy Jagannath, Kangling Zhang
Summary: The study reveals a complex interplay between metabolism, epigenetics, and cell phenotype in macrophage polarization. M1 macrophages showed globally reduced histone acetylation levels but high levels of acetylated amino acids, suggesting a diversion of acetyl-CoA toward acetylated amino acids. Meanwhile, M2 macrophages exhibited high levels of kynurenine and serotonin, which are known to have immune-suppressive effects.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Ritu Mamgain, Sagar Swami, Dhiman Sarkar, Pratibha Srivastava
Summary: Tuberculosis (TB) is a devastating infectious disease that continues to spread among people despite the availability of specific drugs. This study focused on the development of a library of compounds with anti-TB activity using click chemistry. The results showed promising inhibitory potential against both active and dormant strains of Mycobacterium tuberculosis. The compounds also demonstrated ROS generating ability and interaction with a key protein involved in the survival of the bacteria. The findings suggest that these lead molecules could be beneficial in the eradication of TB when combined with existing drugs.