Article
Microbiology
Yue Ma, Yingying Yue, Haihong Jia, Nannan Song, Li Zhai, Weiwei Wang, Cuiling Li, Bingqing Li
Summary: This study reveals that YdiU, an UMPylator, plays a critical role in regulating flagellar biogenesis in Salmonella by UMPylating the FlhC subunit of FlhDC and inhibiting its binding to flagellar genes, thus promoting immune escape.
Article
Microbiology
Xiaojing Fan, Zhiwen Zhao, Tingyan Sun, Wei Rou, Caiying Gui, Tahira Saleem, Xuxu Zhao, Xianyuan Xu, Tao Zhuo, Xun Hu, Huasong Zou
Summary: The study reveals that the regulator CrgA in Ralstonia solanacearum suppresses cell motility by repressing flhDC expression, which consequently affects the expression of fliC to modulate disease development in host plants. This indicates a complex regulation of motility in R. solanacearum and enhances understanding of flagellum regulation in this pathogen.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
So Yeon Cho, Han Byeol Oh, Sung-il Yoon
Summary: Bacterial flagella assembly is regulated by different proteins in a defined order, and in certain bacteria classes, it is controlled by the master regulator FlhDC. The crystal structure of FlhDC from Cupriavidus necator was determined, revealing its DNA-binding capacity and structural features. This study provides insights into the DNA-binding mechanism and functional differences of FlhDC in different bacteria classes.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Guanglu Sun, Zihao Yu, Qianwen Li, Yuanxing Zhang, Mingxiao Wang, Yunhui Liu, Jinze Liu, Lei Liu, Xuping Yu
Summary: Overexpression of the flhDC genes in Escherichia coli can lead to cell death due to excessive production of reactive oxygen species (ROS) from the oxidative phosphorylation pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Sanne Wolput, Angela Makumi, Laura Wicke, Leonard E. Baecker, William Cenens, Yves Briers, Nicolas A. Wenner, Sian V. Owen, Jay C. D. Hinton, Rob Lavigne, Abram Aertsen
Summary: Many phage genes, called ORFans, have no similarity to other genes in current databases. These ORFan genes can have a significant impact on phage propagation and host interactions, but are often unexplored in experiments. In a previous study, we discovered a novel interaction between phage P22 and its host Salmonella Typhimurium, involving the ORFan gene pid. The pid gene is highly expressed in phage carrier cells that harbor a polarly located P22 episome, which segregates unequally among daughter cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biophysics
Daniel Schultz, Mirjana Stevanovic, Lev S. Tsimring
Summary: Cellular responses to toxic compounds require prompt expression of appropriate enzymes regulated by transcription factors. Different regulatory strategies are optimized based on selective pressures, with costs associated with drug, enzyme, and repressor concentrations influencing gene regulation.
BIOPHYSICAL JOURNAL
(2022)
Article
Cell Biology
Karina Perlaza, Ivan Zamora, Wallace F. Marshall
Summary: Two models based on a transcriptional repressor, one produced in the flagellum and one produced in the cell body and sequestered in the growing flagellum, can control flagellar length and reproduce the dynamics of gene expression. Quantitative measurements of gene expression show that mutations of the heterotrimeric kinesin-2, which drives intraflagellar transport (IFT), greatly reduce gene expression during flagellar regeneration, supporting the model in which a repressor is sequestered in the flagellum by IFT. Inhibiting axonemal assembly has a smaller effect on gene expression. The repressor sequestration model allows precursor production to occur when flagella are growing rapidly, representing a form of derivative control.
MOLECULAR BIOLOGY OF THE CELL
(2023)
Article
Biotechnology & Applied Microbiology
Caitlin M. Gionfriddo, Ally Bullock Soren, Ann M. Wymore, D. Sean Hartnett, Mircea Podar, Jerry M. Parks, Dwayne A. Elias, Cynthia C. Gilmour
Summary: This study demonstrates a connection between microbial mercury methylation and arsenic resistance, shedding light on the evolution and transcriptional regulation of hgcAB genes.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Aigera Mendauletova, John A. Latham
Summary: This study investigates the regulation mechanisms of Mycofactocin (MFT) expression in Mycobacterium smegmatis mc(2)155. It is found that the transcription regulator, MftR, binds to the mft promoter region and is induced by long-chain acylCoAs. The results suggest that MFT may play a critical role in fatty acid metabolism or cell wall reorganization.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Rodrigo A. A. Villanueva, Alejandra Loyola
Summary: Hepatitis B virus (HBV) is an enveloped DNA human virus that replicates its genome through reverse transcription. The viral proteome is diversified into isoform proteins through pre- and post-transcriptional gene regulation. The HBx protein, a viral multifunctional protein, has two internal in-frame translational initiation codons, resulting in the expression of two smaller isoform proteins. This article reviews the mechanisms of protein expression regulation during viral replication and explores the regulation of HBV HBx gene and its divergent N-terminal isoform proteins.
Article
Biochemistry & Molecular Biology
Qiujia Li, Xi Chen, Shengnan Zhang, Siyao Shan, Yong Xiang
Summary: Research shows that DOG1 could systematically control the expression of phytohormone-related genes, resulting in significant changes in the endogenous signal intensity of ABA, IAA, GA, BR, and CK, which in turn affect seed dormancy.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Elizabeth Ward, Thibaud T. Renault, Eun A. Kim, Marc Erhardt, Kelly T. Hughes, David F. Blair
MOLECULAR MICROBIOLOGY
(2018)
Article
Oncology
Sebastian Felgner, Dino Kocijancic, Michael Frahm, Ulrike Heise, Manfred Rohde, Kurt Zimmermann, Christine Falk, Marc Erhardt, Siegfried Weiss
Article
Oncology
Sebastian Felgner, Imke Spoering, Vinay Pawar, Dino Kocijancic, Matthias Preusse, Christine Falk, Manfred Rohde, Susanne Haeussler, Siegfried Weiss, Marc Erhardt
INTERNATIONAL JOURNAL OF CANCER
(2020)
Article
Biochemistry & Molecular Biology
Shuichi Nakamura, Yuta Hanaizumi, Yusuke Morimoto, Yumi Inoue, Marc Erhardt, Tohru Minamino, Keiichi Namba
MOLECULAR MICROBIOLOGY
(2020)
Article
Microbiology
Helene Cazzola, Laurine Lemaire, Sebastien Acket, Elise Prost, Luminita Duma, Marc Erhardt, Petra Cechova, Patrick Trouillas, Fady Mohareb, Claire Rossi, Yannick Rossez
Review
Biochemistry & Molecular Biology
Manuel Halte, Marc Erhardt
Summary: The T3SS utilizes proton motive force to export substrate proteins across the inner membrane of pathogenic bacteria. It consists of an export gate and a soluble ATPase complex in the cytoplasm. Recent research on T3SS focuses on energy transduction and protein transport across the cytoplasmic membrane.
Article
Biochemistry & Molecular Biology
Alina Guse, Manuel Halte, Svenja Huesing, Marc Erhardt
Summary: The study revealed that the type-III secretion system of bacterial flagellum has a remarkable substrate specificity during flagellar assembly, accurately secreting the required substrates at different stages, and the specificity switch is unidirectional and irreversible.
MOLECULAR MICROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Haidai Hu, Monica Santiveri, Navish Wadhwa, Howard C. Berg, Marc Erhardt, Nicholas M. Taylor
Summary: This review summarizes recent advances in the understanding of the structure and function of the flagellar stator unit, torque generation, and directional switching of the motor. Recent studies using cryo-electron microscopy and in situ cryo-electron tomography revealed details of the assembly, ion flux pathway, and torque generation mechanism of the stator unit.
TRENDS IN BIOCHEMICAL SCIENCES
(2022)
Article
Multidisciplinary Sciences
Svenja Huesing, Manuel Halte, Ulf van Look, Alina Guse, Eric J. C. Galvez, Emmanuelle Charpentier, David F. Blair, Marc Erhardt, Thibaud T. Renault
Summary: T3SSs are capable of translocating proteins at high speed while preserving the membrane barrier, a structure-function analysis of the T3SS pore complex reveals the mechanisms enabling gating and conformational changes for protein substrate secretion.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Manuel Halte, Mirka E. Woermann, Maxim Bogisch, Marc Erhardt, Natalia Tschowri
Summary: This study presents a novel c-di-GMP biosensor, CensYBL, which can detect fluctuations in the intracellular c-di-GMP levels in bacteria. The functionality of CensYBL in detecting c-di-GMP variations has been demonstrated in Gram-negative model bacteria. The study shows that the CensYBL biosensor is a user-friendly and versatile tool for investigating c-di-GMP fluctuations using different experimental set-ups.
MOLECULAR MICROBIOLOGY
(2022)
Editorial Material
Biochemistry & Molecular Biology
Philipp F. Popp, Marc Erhardt
Summary: This article reports the cryo-EM structures of supercoiled filaments in both bacterial and archaeal motility machines, revealing shared fundamental features of prokaryotic locomotion and representing a prime example of convergent evolution.
Article
Microbiology
Jessica A. Buttress, Manuel Halte, J. Derk te Winkel, Marc Erhardt, Philipp F. Popp, Henrik Strahl
Summary: This paper provides guidance on reliably monitoring membrane potential and its changes in Gram-negative bacteria using the voltage-sensitive dye 3,3'-dipropylthiadicarbocyanine iodide [DiSC 3(5)]. The confounding effects caused by the presence of the outer membrane or measurements performed in buffers are also discussed.
Article
Microbiology
Doaa Osama Saleh, Julia Horstmann, Maria Giralt-Zuniga, Willi R. Weber, Eugen Kaganovitch, Abilash Chakravarthy R. Durairaj, Enrico Klotzsch, Till R. Strowig, Marc Erhardt
Summary: Activation of HilD in Salmonella Typhimurium not only activates the expression of the flagellar master regulator flhDC, but also significantly reduces the bacterial motility. Furthermore, HilD activation leads to upregulation of adhesive structures, activation of the stringent response and depletion of the proton motive force. Based on these findings, a model is proposed where HilD activation allows Salmonella to quickly adjust their motility during the infection process.
Article
Multidisciplinary Sciences
Haidai Hu, Philipp F. Popp, Monica Santiveri, Aritz Roa-Eguiara, Yumeng Yan, Freddie J. O. Martin, Zheyi Liu, Navish Wadhwa, Yong Wang, Marc Erhardt, Nicholas M. I. Taylor
Summary: In this study, the structure of Vibrio PomAB was determined by cryo-electron microscopy, revealing sodium binding sites, and a mechanism for ion translocation and selectivity was proposed through functional experiments and molecular dynamics simulations. The involvement of a dynamic helical motif in PomA was suggested to regulate the distance between PomA subunit cytoplasmic domains, stator unit activation, and torque transmission. Overall, this study provides mechanistic insights into ion selectivity and rotor incorporation of the stator unit of the bacterial flagellum.
NATURE COMMUNICATIONS
(2023)
Article
Biology
Gita Naseri, Hannah Raasch, Emmanuelle Charpentier, Marc Erhardt
Summary: In this study, a set of arabinose-inducible artificial transcription factors (ATFs) were developed using CRISPR/dCas9 and Arabidopsis-derived DNA-binding proteins to control gene expression in E. coli and Salmonella over a wide range of inducer concentrations. The transcriptional output of the ATFs varied greatly, particularly in Salmonella strains rewired for arabinose catabolism. The developed ATFs were used to engineer a Salmonella biosensor strain and regulate beta-carotene biosynthesis in E. coli, resulting in significantly higher production compared to traditional methods.
COMMUNICATIONS BIOLOGY
(2023)