Article
Biochemistry & Molecular Biology
Eiji Tamai, Mitsugu Yamada, Takuya Ishida, Nayu Arimura, Risa Matsunami, Hiroshi Sekiya, Shigehiro Kamitori
Summary: Sortase-mediated pili are important for the initial adhesion of bacterial cells to host tissues. A Gram-positive bacterium, Clostridium perfringens, has major and minor/tip pilins for this process. The minor/tip pilin (CppB) with a collagen-binding motif adopts an L-shaped structure and has a small beta-sheet that provides a favorable binding site for collagen peptide.
Article
Microbiology
Zhifeng Sun, Mingmin Lu, Hyun Lillehoj, Youngsub Lee, Doyun Goo, Baohong Yuan, Xianghe Yan, CCharles Li
Summary: This study found that the collagen adhesion capability of Clostridium perfringens, the etiological agent for necrotic enteritis in broiler chickens, is correlated with its pathogenicity, especially for C. perfringens strains carrying crucial virulence factors such as netB, cnaA, and tpeL. These results suggest that the presence of the cnaA gene may be associated with the virulence of C. perfringens.
Article
Urology & Nephrology
Benjamin Green, Ashley Flores, Jacob Warner, Tobias Kohler, Sevann Helo, Landon Trost
Summary: This study compared the efficacy of collagenase Clostridium histolyticum with surgery for treating Peyronie's disease. The results showed that patients treated with collagenase Clostridium histolyticum were more satisfied with improvements in erectile function, penile length, and sensation, and experienced fewer adverse events. However, patients who underwent surgery had better curve improvements but shorter penile length and a higher incidence of adverse events.
JOURNAL OF UROLOGY
(2023)
Review
Food Science & Technology
Kyle S. Gregory, K. Ravi Acharya
Summary: Botulinum neurotoxins (BoNTs) are highly potent neurotoxins that cause neuromuscular paralysis by cleaving SNARE complex proteins. They have high affinity and specificity for neuromuscular junctions. This review focuses on the structural analysis of the cell-binding domain of different BoNT subtypes, providing insights into their functions and potential therapeutic applications.
Article
Biochemistry & Molecular Biology
Shibani Bhattacharya, Anthony Palillo
Summary: This study investigates the dynamic and structural determinants of CtA binding in the PEP domain of PCAT1, revealing insights into the mechanism of peptide cleavage optimization. Through nuclear magnetic resonance spectroscopy, the researchers characterized the interactions between CtA, PEP domain, and PCAT1 transporter, providing a detailed analysis of substrate binding and enzyme activity regulation. The findings shed light on the ATP-mediated association between PEP and transmembrane domains, offering fresh understanding of the molecular mechanisms underlying peptide cleavage.
Article
Food Science & Technology
Kyle S. Gregory, Tejaswini B. Mahadeva, Sai Man Liu, K. Ravi Acharya
Summary: Botulinum neurotoxins are toxic substances that can cause botulism, but they can also be used to treat neuromuscular conditions. This study reveals the crystal structures of the cell binding domain of botulinum neurotoxin A2 and its interactions with the receptor ganglioside GD1a. The findings provide important insights into the binding mechanism between the toxin and its receptor.
Article
Biophysics
Alaa Al-Shaer, Aaron Lyons, Yoshihiro Ishikawa, Billy G. Hudson, Sergei P. Boudko, Nancy R. Forde
Summary: The mechanical properties of collagen IV, an integral component of basement membranes, have been less studied compared to fibril-forming collagens. The presence of interruptions in the triple-helix-defining sequence along its collagenous domain leads to highly heterogeneous mechanics in collagen IV, while sequence variations within the triple helix contribute to variable flexibility in collagen III. Physiological changes in pH and chloride concentration do not alter the flexibility of collagen IV, indicating that environmental factors may not control its compaction during secretion.
BIOPHYSICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Weibin Gong, Qihui Liang, Yufeng Tong, Sarah Perrett, Yingang Feng
Summary: RBBP1 is involved in gene regulation, epigenetic regulation, and disease processes. The ARID domain in RBBP1 plays a key role in DNA binding and gene suppression, with unexpected involvement of the disordered acidic loop L12.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Emma Stepinac, Nicolas Landrein, Daria Skwarzynska, Patrycja Wojcik, Johannes Lesigang, Iva Lucic, Cynthia Y. He, Melanie Bonhivers, Derrick R. Robinson, Gang Dong
Summary: Extended synaptotagmins (E-Syts) like TbE-Syt in the protist parasite Trypanosoma brucei contain two C2 domains and bind lipids through Ca2+ and PI(4,5)P-2 dependent or independent mechanisms, facilitating lipid transfer between the ER membrane and the plasma membrane.
Article
Urology & Nephrology
John T. Sigalos, Michelle K. Li, Dar A. Yoffe, Neilufar Modiri, Ming-Yeah Y. Hu, Dyvon T. Walker, Thomas W. Gaither, Alvaro Santamaria, Keith V. Regets, Sriram V. Eleswarapu, Jesse N. Mills
Summary: A study found that 5.8% of male patients receiving collagenase injections for Peyronie's disease experienced postinjection acute lower back pain, with the rate increasing to 1.3% in those who completed at least 1 full course of treatment. The incidence of pain increases with the number of treatment rounds.
JOURNAL OF UROLOGY
(2022)
Article
Chemistry, Multidisciplinary
Akari Nishi, Hikaru Matsui, Azumi Hirata, Atsushi Mukaiyama, Shun-ichi Tanaka, Takuya Yoshizawa, Hiroyoshi Matsumura, Ryota Nomura, Kazuhiko Nakano, Kazufumi Takano
Summary: In this study, the researchers focused on the structure and activity of collagen-binding proteins from Streptococcus mutans. They found that the N1 and N2 domains as well as the linker region are important for collagen binding. Understanding these mechanisms could potentially lead to the development of CBD inhibitors for infective endocarditis caused by S. mutans.
CHEMISTRY-SWITZERLAND
(2023)
Article
Oncology
Yannick Fabian Diehm, Katharina Marstaller, Anna-Maria Seckler, Martin Reinhold Berger, Michael Zepp, Matthias Martin Gaida, Julia Thome, Dimitra Kotsougiani-Fischer, Ulrich Kneser, Sebastian Fischer
Summary: This study investigated the impact of CCH treatment on breast cancer cells. The results showed that CCH had an effect on the proliferation and wound healing of breast cancer cells in vitro and in vivo, but did not significantly change the cell cycle and apoptosis. No evidence of metastasis was observed in the in vivo experiments, and no relevant alterations in metastasis-related genes were detected. Therefore, CCH does not have an impact on tumor growth or metastasis formation.
Review
Medicine, General & Internal
Dehong Cao, Jinze Li, You Lu, Yin Huang, Bo Chen, Zeyu Chen, Yinzhi Shen, Liangren Liu, Qiang Wei
Summary: Collagenase Clostridium Histolyticum (CCH) has shown significant efficacy in treating Peyronie's disease (PD), effectively relieving penile curvature deformity and symptom bother, although there is no difference in penile pain scores and treatment-related adverse events compared to placebo.
FRONTIERS IN MEDICINE
(2022)
Review
Cell Biology
Fatima Trejo, Stephanie Elizalde, Adriana Mercado, Gerardo Gamba, Paola de losHeros
Summary: The aim of this review is to analyze the cryo-EM data of the SLC12A superfamily of membrane transporters and their structural elements, as well as their role in physiological processes and pathophysiological conditions. The review also discusses the potential of using these structural elements for the development of novel treatments and to gain mechanistic insight into cation-coupled chloride cotransporters.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Cell Biology
Roza K. Przanowska, Chase A. Weidmann, Shekhar Saha, Magdalena A. Cichewicz, Kate N. Jensen, Piotr Przanowski, Patrick S. Irving, Kevin A. Janes, Michael J. Guertin, Kevin M. Weeks, Anindya Dutta
Summary: Experimental probing revealed that MUNC lncRNA has complex structure-driven functions, including induction of promyogenic genes, binding genomic sites and gene expression regulation, and interacting with the cohesin complex. This highlights the importance of experimentally determined structures for understanding structure-function relationships in lncRNAs.
Article
Biotechnology & Applied Microbiology
McKinzie Fruchtl, Joshua Sakon, Robert Beitle
BIOTECHNOLOGY PROGRESS
(2015)
Article
Biochemical Research Methods
Ryan Bauer, Katarzyna Janowska, Kelly Taylor, Brad Jordan, Steve Gann, Tomasz Janowski, Ethan C. Latimer, Osamu Matsushita, Joshua Sakon
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2015)
Article
Biotechnology & Applied Microbiology
McKinzie Fruchtl, Joshua Sakon, Robert Beitle
JOURNAL OF BIOTECHNOLOGY
(2016)
Article
Chemistry, Multidisciplinary
Nalinikanth Kotagiri, Joshua Sakon, Haewook Han, Vladimir P. Zharov, Jin-Woo Kim
Article
Oncology
Ranjitha Katikaneni, Tulasi Ponnapakkam, Osamu Matsushita, Joshua Sakon, Robert Gensure
Review
Pharmacology & Pharmacy
T. Ponnapakkam, R. Katikaneni, J. Sakon, R. Stratford, R. C. Gensure
DRUG DISCOVERY TODAY
(2014)
Article
Biochemistry & Molecular Biology
Perry Caviness, Ryan Bauer, Keisuke Tanaka, Katarzyna Janowska, Jeffrey Randall Roeser, Dawn Harter, Jes Sanders, Christopher Ruth, Osamu Matsushita, Joshua Sakon
Article
Chemistry, Medicinal
Robert Stratford, Christopher Vu, Joshua Sakon, Ranjitha Katikaneni, Robert Gensure, Tulasi Ponnapakkam
JOURNAL OF PHARMACEUTICAL SCIENCES
(2014)
Article
Materials Science, Paper & Wood
Kalavathy Rajan, Angele Djioleu, Gurshagan Kandhola, Nicole Labbe, Joshua Sakon, Danielle Julie Carrier, Jin-Woo Kim
Article
Materials Science, Paper & Wood
Gurshagan Kandhola, Angele Djioleu, Kalavathy Rajan, Joseph Batta-Mpouma, Nicole Labbe, Joshua Sakon, Benjamin A. Babst, Anindya Ghosh, Danielle Julie Carrier, Jin-Woo Kim
Summary: Cellulose nanocrystals (CNCs) are renewable and biocompatible nanomaterials with unique properties. The production and properties of CNCs from different wood species were investigated. The results showed that the type of wood species had an impact on the yield, purity, and properties of CNCs. This study provides insights into optimizing CNC production and usage for different applications.
Article
Biochemistry & Molecular Biology
Jin-Woo Kim, Joseph Batta-Mpouma, Gurshagan Kandhola, Joshua Sakon
Summary: Cellulose nanocrystals (CNCs) can be crosslinked to form nanoparticle-based hydrogels with tunable physicochemical properties, which improves the instability issue of CNCs in moist environments. The strength of intermolecular hydrogen bonding can be controlled by the type and concentration of crosslinkers as well as the CNC concentration. By changing the crosslinking conditions, the surface chemistry of CNCs can be transformed from hydrophilic to hydrophobic, and the topology and aqueous stability of self-assembled structures can be tuned.
Article
Biotechnology & Applied Microbiology
Gurshagan Kandhola, Angele Djioleu, Kalavathy Rajan, Nicole Labbe, Joshua Sakon, Danielle Julie Carrier, Jin-Woo Kim
BIORESOURCES AND BIOPROCESSING
(2020)
Meeting Abstract
Biochemistry & Molecular Biology
Ryan Bauer, Katarzyna Janowska, Keisuke Tanaka, Osamu Matsushita, Joshua Sakon
Meeting Abstract
Biochemistry & Molecular Biology
Perry Carter Caviness, Takaki Koide, Osamu Matsushita, Joshua Sakon
Meeting Abstract
Biochemistry & Molecular Biology
Christopher Eric Ruth, Joshua Sakon