期刊
JOURNAL OF BACTERIOLOGY
卷 194, 期 17, 页码 4669-4676出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.00957-12
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资金
- Wellcome Trust
- New College (Oxford)
SMC (structural maintenance of chromosomes) complexes function ubiquitously in organizing and maintaining chromosomes. Functional fluorescent derivatives of the Escherichia coli SMC complex, MukBEF, form foci that associate with the replication origin region (on). MukBEF impairment results in mispositioning of on and other loci in steady-state cells. These observations led to an earlier proposal that MukBEF positions new replicated sister oris. We show here that MukBEF generates and maintains the cellular positioning of chromosome loci independently of DNA replication. Rapid impairment of MukBEF function by depleting a Muk component in the absence of DNA replication leads to loss of MukBEF foci as well as mispositioning of on and other loci, while rapid Muk synthesis leads to rapid MukBEF focus formation but slow restoration of normal chromosomal locus positioning.
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