Article
Microbiology
Jia-Ming Tsai, Hsin-Wei Kuo, Winton Cheng
Summary: This study conducted a retrospective screening of 32 bacterial isolates from Chinese soft-shelled turtles and found that all isolates belong to the pathogenic variant Bacillus tropicus str. JMT. The results of this study indicate that the geographic distribution and host range of B. tropicus were previously underestimated.
Article
Microbiology
Grace Taylor-Joyce, Shathviga Manoharan, Thomas A. Brooker, Carmen Sara Hernandez-Rodriguez, Les Baillie, Petra C. F. Oyston, Alexia Hapeshi, Nicholas R. Waterfield
Summary: This study investigates the influence of pBCX01 and temperature on the lifestyle of B. cereus G9241, using transcriptomic analysis and the study of spore formation. The results show that pBCX01 has a negative effect on cell metabolism genes at 37℃, but positively affects the transcription of transmembrane proteins. Additionally, the study reveals that pBFH_1 is highly expressed at 37℃ and leads to the production of Siphoviridae-like phage particles in B. cereus G9241.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Cecile Boutonnet, Sebastien Lyonnais, Beatrice Alpha-Bazin, Jean Armengaud, Alice Chateau, Catherine Duport
Summary: The dynamics of SL2 and EA1 expression are key determinants of the surface properties of B. cereus AH187, and the S-layer may contribute to the survival of B. cereus in starvation conditions.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Patrick Dawson, Johanna S. Salzer, Caroline A. Schrodt, Karl Feldmann, Cari B. Kolton, Jay E. Gee, Chung K. Marston, Christopher A. Gulvik, Mindy G. Elrod, Aaron Villarma, Rita M. Traxler, Maria E. Negron, Kate A. Hendricks, Heather Moulton-Meissner, Laura J. Rose, Paul Byers, Kathryn Taylor, Daphne Ware, Gary A. Balsamo, Theresa Sokol, Bret Barrett, Erica Payne, Saad Zaheer, Ga On Jung, Stephen Long, Ricardo Quijano, Lindsey LeBouf, Briana O'Sullivan, Erin Swaney, James M. Antonini, Marie A. de Perio, Zachary Weiner, William A. Bower, Alex R. Hoffmaster
Summary: Investigations of two cases of welder's anthrax found that the clinical isolates from one patient matched bacterial strains recovered from their work-related clothing and gear, as well as the worksite soil. All welder's anthrax cases should be investigated to determine the source of exposure.
Article
Microbiology
Shathviga Manoharan, Grace Taylor-Joyce, Thomas A. Brooker, Carmen Sara Hernandez Rodriguez, Alexia Hapeshi, Victoria Baldwin, Les Baillie, Petra C. F. Oyston, Nicholas R. Waterfield
Summary: Bacillus cereus G9241, isolated from a welder with an anthrax-like infection, demonstrates similar cytotoxic and hemolytic activity to Bacillus anthracis at 25 degrees C but not at 37 degrees C. The differential expression of the protease involved in processing PlcR quorum sensing activator molecule PapR appears to be the main factor determining the production of PlcR-regulated toxins at 37 degrees C. This study provides insights into how B. cereus G9241 can switch between B. cereus and B. anthracis-like phenotypes depending on temperature.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Ephrem Debebe Zegeye, Brajabandhu Pradhan, Ann-Katrin Llarena, Marina Aspholm
Summary: The spores of various Bacillus cereus species are covered with hair/pilus-like appendages, including S-Ena and L-Ena structures, which are key subjects of study. Ena homologs are widely distributed among species and may play important functional roles, necessitating further research on their impact on spore-environment interactions. A better understanding of the roles of these structures in spore biological functions and their physicochemical properties is needed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Eva Maria Kalbhenn, Markus Kranzler, Agnieszka Gacek-Matthews, Gregor Grass, Timo D. Stark, Elrike Frenzel, Monika Ehling-Schulz
Summary: Bacillus cereus causes emetic type foodborne disease through production of the small peptide toxin cereulide. The CesNRPS multienzyme machinery, encoding Ces nonribosomal peptide synthetase, is located on a plasmid designated pCER270. Chromosomal transcriptional factors CodY and AbrB control ces transcription. In addition, a plasmid-encoded transcription factor PagRBc, homologous to PagR in Bacillus anthracis, also plays an important role in the regulation of cereulide synthesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Phillip S. Coburn, Frederick C. Miller, Austin L. LaGrow, Huzzatul Mursalin, Anna Gregory, Aaron Parrott, Daniel Astley, Michelle C. Callegan
Summary: This study compared ocular and gastrointestinal isolates of Bacillus cereus (Bc) in terms of genotypes and virulence in endophthalmitis. The results showed that while gastrointestinal isolates were more hemolytic, ocular isolates had a higher frequency of virulence-related genes. However, there were no significant differences in retinal function retention and intraocular growth between the two groups.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biochemical Research Methods
Cecile Boutonnet, Christian Ginies, Beatrice Alpha-Bazin, Jean Armengaud, Alice Chateau, Catherine Duport
Summary: The S-layer of Bacillus cereus has a significant impact on metabolism and the response during the stationary phase.
JOURNAL OF PROTEOMICS
(2023)
Article
Microbiology
Laura M. Carroll, Chung K. Marston, Cari B. Kolton, Christopher A. Gulvik, Jay E. Gee, Zachary P. Weiner, Jasna Kovac
Summary: This study characterized three B. cereus s.l. isolates associated with two anthrax cases in welders in the United States in 2020. The genomes of these isolates belonged to different species, with two being closely related to B. tropicus and one being most similar to B. anthracis.
Article
Biochemistry & Molecular Biology
Veronika Walser, Markus Kranzler, Corinna Dawid, Monika Ehling-Schulz, Timo D. Stark, Thomas F. Hofmann
Summary: The emetic Bacillus cereus toxin cereulide poses a significant safety risk in the food industry, causing emesis and nausea after consumption of contaminated foods. Recently, various isocereulides, similar to cereulide, have been identified in B. cereus-contaminated food samples. This study successfully isolated and elucidated the chemical structures of seven new isocereulides and investigated their toxicity.
Article
Microbiology
Qiubin Huang, Huiping Liu, Juanmei Zhang, Shaowei Wang, Fengying Liu, Chengdie Li, Gang Wang
Summary: Bacillus cereus 0-9 encodes three genes annotated as amylases, among which only the amyS gene produces extracellular amylase. This gene influences the colonization abilities of the bacterium in wheat roots and its biocontrol abilities against Rhizoctonia cerealis.
Article
Immunology
Lu-Ying Wang, Xu-Peng Wang, Jin-Meng Lv, Yu-Dong Shan, Shi-Yan Jia, Zhi-Fang Yu, Hui-Tao Miao, Yue Xin, Dong-Xue Zhang, Li-Min Zhang
Summary: This study found that surgery and anesthesia can induce neuroinflammation and cognitive deficits, which are linked to the activation of the NLRP3-GABA pathway in the hippocampus of aged mice. The use of an NLRP3 inhibitor, selegiline, was shown to improve these conditions.
JOURNAL OF NEUROINFLAMMATION
(2023)
Article
Food Science & Technology
Shaoying Gong, Chaoqin Jiao, Biqi Liu, Wenxuan Qu, Ling Guo, Yujun Jiang
Summary: In this study, phytochemicals in beetroot extract (BE) were analyzed and it was found to contain a total of 745 phytochemicals. Nine polyphenols and 19 flavonoids with growth-inhibiting activity against Bacillus cereus were identified. The minimum inhibitory concentration of BE against B. cereus was determined to be 15 mg/mL. Furthermore, BE was found to induce apoptosis-like death in B. cereus by depleting reactive oxygen species.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Chemistry, Analytical
Jingnan Meng, Haoran Shen, Lin Luo, Juan Wang, Zhen-Lin Xu, Yingju Liu
Summary: A new method was developed to detect the presence of B. cereus. By using 3D MnO2-Au nanoflowers and blue carbon quantum dots as fluorescent signal molecules, a linear relationship between the ratiometric fluorescence signal and B. cereus concentration was established. This method is faster and more sensitive compared to traditional detection methods, making it a promising tool for convenient and effective B. cereus screening.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Multidisciplinary Sciences
Fernanda L. Paganelli, Misha Luyer, C. Marijn Hazelbag, Hae-Won Uh, Malbert R. C. Rogers, Danielle Adriaans, Roos-Marijn Berbers, Antoni P. A. Hendrickx, Marco C. Viveen, James A. Groot, Marc J. M. Bonten, Ad C. Fluit, Rob J. L. Willems, Helen L. Leavis
SCIENTIFIC REPORTS
(2019)
Article
Microbiology
Janetta Top, Antoni P. A. Hendrickx, Marleen T. J. van Ampting, Kees van Limpt, Jan Knol, Denise van de Kamer, Johanna C. Braat, Marco Viveen, Malbert R. Rogers, Hans Kemperman, Rob J. L. Willems, Fernanda L. Paganelli
Article
Multidisciplinary Sciences
Antoni P. A. Hendrickx, Fabian Landman, Angela de Haan, Dyogo Borst, Sandra Witteveen, Marga G. van Santen-Verheuvel, Han G. J. van der Heide, Leo M. Schouls
SCIENTIFIC REPORTS
(2020)
Article
Public, Environmental & Occupational Health
Cornelia C. H. Wielders, Leo M. Schouls, Sjoukje H. S. Woudt, Daan W. Notermans, Antoni P. A. Hendrickx, Jacinta Bakker, Ed J. Kuijper, Annelot F. Schoffelen, Sabine C. de Greeff
Summary: Carbapenem resistance of Enterobacterales in the Netherlands remains low, but the number of CPE isolates has slightly increased. Recent hospitalization abroad is the main risk factor for acquiring CPE.
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL
(2022)
Article
Infectious Diseases
Monika Fliss, Charlotte H. S. B. van den Berg, Ed Kuijper, Daan W. Notermans, Antoni P. A. Hendrickx, Mirthe H. Schoots, Erik Bathoorn
Summary: Two severe community-acquired pulmonary infections caused by hypervirulent Klebsiella pneumoniae were reported in patients without recent travel history in the Netherlands. The bacterial isolates were closely related to a typical archetype ST23 hvKp reference strain. Surveillance programs may consider including isolates from community-acquired pneumonia caused by K. pneumoniae.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2022)
Article
Immunology
Karuna E. W. Vendrik, Tim G. J. de Meij, Arend Bokenkamp, Rogier E. Ooijevaar, Bas Groenewegen, Antoni P. A. Hendrickx, Elisabeth M. Terveer, Ed J. Kuijper, Joffrey van Prehn
Summary: Fecal microbiota transplantation (FMT) can transmit antibiotic-susceptible bacteria causing urinary tract infections (UTIs), highlighting the importance of long-term follow-up and fecal screening after FMT.
OPEN FORUM INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Daan W. Notermans, Annelot F. Schoffelen, Fabian Landman, Cornelia C. H. Wielders, Sandra Witteveen, Varisha A. Ganesh, Marga Van Santen-Verheuvel, Sabine C. de Greeff, Ed J. Kuijper, Antoni P. A. Hendrickx
Summary: Urine-associated Escherichia coli ST38 producing OXA-244 harboring putative uropathogenicity factors emerged in the Netherlands.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Microbiology
Sandra Witteveen, Antoni P. A. Hendrickx, Angela de Haan, Daan W. Notermans, Fabian Landman, Marga G. Van Santen-Verheuvel, Sabine C. de Greeff, Ed J. Kuijper, Noortje M. van Maarseveen, Saara Vainio, Leo M. Schouls
Summary: The study reveals the circulation of MRSArg in the Netherlands since at least 2008. Despite being distinct from MRSA, MRSArg shares a comparable population structure and similar resistance and virulence genes. The Dutch national MRSA surveillance has expanded to include other methicillin-resistant members of the S. aureus complex, such as S. argenteus and Staphylococcus schweitzeri.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Antoni P. A. Hendrickx, Rogier P. Schade, Fabian Landman, Thijs Bosch, Leo M. Schouls, Karin van Dijk
Summary: The study conducted a detailed analysis of three carbapenemase-producing A. ursingii strains, identifying similar plasmids carrying the blaIMP-4 and bla OXA-58 genes, suggesting a potential origin from A. johnsonii.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Article
Infectious Diseases
Cindy Dierikx, Paul Hengeveld, Sandra Witteveen, Angela van Hoek, Marga van Santen-Verheuvel, Margriet Montizaan, Marja Kik, Miriam Maas, Leo Schouls, Antoni Hendrickx, Dutch MRSA surveillance study grp, Engeline van Duijkeren
Summary: This study compared the genetic characteristics of mecC-MRSA from hedgehogs and humans using next-generation sequencing. The results showed that mecC-MRSA from both species mainly belonged to two clonal complexes, suggesting a potential common source. However, there was no firm evidence for recent zoonotic transmission of mecC-MRSA from hedgehogs to humans, and further studies are needed to understand the role of hedgehogs in the occurrence of mecC-MRSA in humans.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Rita Elias, Anton Spadar, Antoni P. A. Hendrickx, Remy A. Bonnin, Laurent Dortet, Margarida Pinto, Jody E. Phelan, Isabel Portugal, Susana Campino, Gabriela Jorge da Silva, Taane G. Clark, Aida Duarte, Joao Perdigao
Summary: This study characterized the genomic diversity and international dissemination of CRKP strains from tertiary care hospitals in Lisbon, Portugal. Two genomic clusters, ST13/GC1 and ST17/GC2, were identified. Additionally, an emerging OXA-181/ST17-producing strain in Portugal and the ongoing international dissemination of a KPC-3/ST13-producing clone were highlighted.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Letter
Infectious Diseases
Casper Jamin, Michael S. M. Brouwer, Kees T. Veldman, Erik Beuken, Sandra Witteveen, Fabian Landman, Edou Heddema, Paul H. M. Savelkoul, Lieke van Alphen, Antoni P. A. Hendrickx
JAC-ANTIMICROBIAL RESISTANCE
(2023)
Article
Medicine, Research & Experimental
Leo M. Schouls, Sandra Witteveen, Marga van Santen-Verheuvel, Angela de Haan, Fabian Labdman, Han van der Heide, Ed J. Kuijper, Daan W. Notermans, Thijs Bosch, Antoni P. A. Hendrickx, A. L. E. van Arkel, M. A. Leversteijn-van Hall, W. Van den Bijllaardt, R. van Mansfeld, K. van Dijk, B. Zwart, B. M. W. Diederen, H. Berkhout, D. W. Notermans, A. Ott, K. Waar, W. Ang, J. da Silva, A. L. M. Vlek, A. G. M. Buiting, L. G. M. Bode, A. Jansz, S. Paltansing, A. J. van Griethuysen, J. R. Lo Ten Foe, M. J. C. A. van Trijp, M. Wong, A. E. Muller, M. P. M. van der Linden, M. van Rijn, S. B. Debast, E. Kolwijck, N. Al Naiemi, T. Schulin, S. Dinant, S. P. van Mens, D. C. Melles, J. W. T. Cohen Stuart, P. Gruteke, A. van Dam, I. Maat, B. Maraha, J. C. Sinnige, E. van der Vorm, M. P. A. van Meer, N. van Maarseveen, E. de Jong, S. J. Vainio, E. Heikens, M. den Reijer, J. W. Dorigo-Zetsma, A. Troelstra, E. Bathoorn, J. de Vries, D. W. van Dam, E. I. G. B. de Brauwer, R. Steingrover
Summary: This study in the Netherlands characterized 43,321 MRSA isolates obtained between 2008 and 2019. Genomic changes occur in the MRSA population, with an increasing proportion of PVL-positive MRSA.
COMMUNICATIONS MEDICINE
(2023)
Article
Infectious Diseases
C. M. Dierikx, A. P. Meijs, P. D. Hengeveld, F. R. M. van der Klis, J. van Vliet, E. F. Gijsbers, M. Rozwandowicz, A. H. A. M. van Hoek, A. P. A. Hendrickx, J. Hordijk, E. Van Duijkeren
Summary: This study investigated the prevalence of mcr-mediated colistin-resistant Escherichia coli and Klebsiella pneumoniae (ColR-E/K) in veterinary healthcare workers and the general population in the Netherlands. The results showed a low prevalence of mcr-mediated ColR-E/K in both populations. Furthermore, mcr-8 was identified for the first time in the Netherlands. Therefore, surveillance of colistin resistance and its underlying mechanisms in humans, livestock, and food is important for identifying emerging trends.
JAC-ANTIMICROBIAL RESISTANCE
(2022)
Article
Infectious Diseases
Antoni P. A. Hendrickx, Sylvia Debast, Maria Perez-Vazquez, Annelot F. Schoffelen, Daan W. Notermans, Fabian Landman, Cornelia C. H. Wielders, Javier E. Canada Garcia, Jacky Flipse, Angela de Haan, Sandra Witteveen, Marga van Santen-Verheuvel, Sabine C. de Greeff, Ed Kuijper, Leo M. Schouls
Summary: The genetic cluster of Enterobacter cloacae complex isolates collected in Dutch national surveillance from 2015-20 revealed a lineage producing carbapenemase, carrying multiple antibiotic resistance genes. The EclCluster-013 isolates, originating from three different hospitals in the Netherlands, belonged to the globally disseminated ST78 lineage and carried an IncH12 plasmid containing bla(VIM-1) carbapenemase and the novel mcr-9 colistin resistance gene. These MDR strains were susceptible to meropenem and colistin.
JAC-ANTIMICROBIAL RESISTANCE
(2021)