4.4 Article

Genome Sequence of the Soybean Symbiont Sinorhizobium fredii HH103

期刊

JOURNAL OF BACTERIOLOGY
卷 194, 期 6, 页码 1617-1618

出版社

AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.06729-11

关键词

-

资金

  1. Bundesministerium fur Forschung und Technologie, Germany [0313805A]
  2. Spanish Ministry of Science and Innovation [BIO2008-05736-C02-01/02]

向作者/读者索取更多资源

Sinorhizobium fredii HH103 is a fast-growing rhizobial strain that is able to nodulate legumes that develop determinate nodules, e.g., soybean, and legumes that form nodules of the indeterminate type. Here we present the genome of HH103, which consists of one chromosome and five plasmids with a total size of 7.22 Mb.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.4
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

Article Microbiology

CRP-Like Transcriptional Regulator MrpC Curbs c-di-GMP and 3 ',3 '-cGAMP Nucleotide Levels during Development in Myxococcus xanthus

Sofya Kuzmich, Patrick Blumenkamp, Doreen Meier, Dobromir Szadkowski, Alexander Goesmann, Anke Becker, Lotte Sgaard-Andersen

Summary: The second messenger c-di-GMP plays an important role in both stages of Myxococcus xanthus' nutrient-regulated biphasic life cycle, with the formation of predatory swarms in the presence of nutrients and spore-filled fruiting bodies in the absence of nutrients. However, different enzymes are involved in c-di-GMP synthesis and degradation during distinct life cycle stages.
Article Microbiology

The nodD1 Gene of Sinorhizobium fredii HH103 Restores Nodulation Capacity on Bean in a Rhizobium tropici CIAT 899 nodD1/nodD2 Mutant, but the Secondary Symbiotic Regulators nolR, nodD2 or syrM Prevent HH103 to Nodulate with This Legume

Francisco Fuentes-Romero, Pilar Navarro-Gomez, Paula Ayala-Garcia, Isamar Moyano-Bravo, Francisco-Javier Lopez-Baena, Francisco Perez-Montano, Francisco-Javier Ollero-Marquez, Sebastian Acosta-Jurado, Jose-Maria Vinardell

Summary: This study demonstrates that the nodD1 gene of Sinorhizobium fredii HH103 can restore the nodulation capacity of a double nodD1/nodD2 mutant of Rhizobium tropici CIAT 899 in bean plants. Mutation of certain symbiotic regulatory genes affects the symbiotic behavior and nitrogenase activity of HH103, leading to the formation of colonized nodules.

MICROORGANISMS (2022)

Article Multidisciplinary Sciences

Frequency modulation of a bacterial quorum sensing response

Vera Bettenworth, Simon van Vliet, Bartosz Turkowyd, Annika Bamberger, Heiko Wendt, Matthew McIntosh, Wieland Steinchen, Ulrike Endesfelder, Anke Becker

Summary: In quorum sensing, bacteria use frequency-modulated pulsing mechanism to make collective decisions. Each cell's physiological state and behavioral needs are encoded in the pulse frequency of autoinducer synthase gene expression, and the integration of pulse frequencies with other cells determines the initiation of collective behavior.

NATURE COMMUNICATIONS (2022)

Article Biology

NT-CRISPR, combining natural transformation and CRISPR-Cas9 counterselection for markerless and scarless genome editing in Vibrio natriegens

Daniel Stukenberg, Josef Hoff, Anna Faber, Anke Becker

Summary: The bacterium Vibrio natriegens has gained attention for its potential in genetic research and biotechnology. This article presents a method called NT-CRISPR, which combines natural transformation with CRISPR-Cas9 counterselection to achieve efficient genome editing and cell killing. This method allows for scarless, markerless edits with single-base precision.

COMMUNICATIONS BIOLOGY (2022)

Article Biochemistry & Molecular Biology

Surface Motility Regulation of Sinorhizobium fredii HH103 by Plant Flavonoids and the NodD1, TtsI, NolR, and MucR1 Symbiotic Bacterial Regulators

Cynthia Alias-Villegas, Francisco Fuentes-Romero, Virginia Cuellar, Pilar Navarro-Gomez, Maria J. Soto, Jose-Maria Vinardell, Sebastian Acosta-Jurado

Summary: Bacteria can spread on surfaces to colonize new environments. Flavonoids and bacterial transcriptional activator NodD have an impact on the surface motility of Sinorhizobium fredii HH103.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

Review Biochemistry & Molecular Biology

The Rhizobial Type 3 Secretion System: The Dr. Jekyll and Mr. Hyde in the Rhizobium-Legume Symbiosis

Irene Jimenez-Guerrero, Carlos Medina, Jose Maria Vinardell, Francisco Javier Ollero, Francisco Javier Lopez-Baena

Summary: Rhizobia establish a symbiotic association with legumes, forming plant nodules where they fix atmospheric nitrogen into ammonia. The type 3 secretion system (T3SS) plays a crucial role in several rhizobium-legume symbioses by injecting effectors into host cells to alter host pathways or suppress host defense responses.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

Article Multidisciplinary Sciences

Legume NCRs and nodule-specific defensins of actinorhizal plants-Do they share a common origin?

Marco Guedes Salgado, Irina Demina, Pooja Jha Maity, Anurupa Nagchowdhury, Andrea Caputo, Elizaveta Krol, Christoph Loderer, Gunther Muth, Anke Becker, Katharina Pawlowski

Summary: Datisca glomerata is an actinorhizal plant that forms root nodules in symbiosis with actinobacteria. In this study, a highly expressed gene family encoding nodule-specific defensin-like peptides was investigated. The results showed that the defensin domain of DgDef1 has cytotoxic effects and can induce the expression of oxidative stress-related genes. These findings suggest that nodule-specific defensin-like peptides were part of the original toolkit for root nodule formation and have been maintained in actinorhizal lineages.

PLOS ONE (2022)

Article Biotechnology & Applied Microbiology

Identification of Ensifer meliloti genes required for survival during peat-based bioinoculant maturation by STM-seq

Mauricio J. Lozano, Ezequiel G. Mogro, M. Eugenia Salas, Sofia A. Erdozain, Nicolas E. Zuber, Anke Becker, Antonio Lagares

Summary: Through genome-wide screening of Ensifer meliloti mutants, it was found that persistence in peat inoculants involves a complex phenotype connected to diverse cellular activities, mainly related to satisfying bacterial nutrition requirements and coping with specific stresses. These results provide a base knowledge that could be used to understand the survival mechanisms used by rhizobia during the maturation of peat-based inoculants and to improve inoculant formulations.

JOURNAL OF BIOTECHNOLOGY (2023)

Article Immunology

Screening for eukaryotic motifs in Legionella pneumophila reveals Smh1 as bacterial deacetylase of host histones

Stefanie M. Herbel, Lambert Moyon, Marvin Christ, Eslam M. Elsayed, Brian E. Caffrey, Silke Malmsheimer, Iwan Grin, Kerstin Hoffmann, Kristin Surmann, Sascha Blankenburg, Anna Lena Jung, Christina E. Herkt, Marco Borso, Beyza Bozdag, Axel Imhof, Anke Becker, Samuel Wagner, Gert Bange, Uwe Voelker, Wilhelm Bertrams, Annalisa Marsico, Bernd Schmeck

Summary: Legionella pneumophila is a common pathogen that causes pneumonia and it acquires eukaryote-like protein motifs that contribute to its pathogenicity. The study identified a bacterial factor, Smh1, with histone deacetylase activity that moderates host gene expression and promotes bacterial replication.

VIRULENCE (2022)

Article Biology

Non-Ionic Osmotic Stress Induces the Biosynthesis of Nodulation Factors and Affects Other Symbiotic Traits in Sinorhizobium fredii HH103

Francisco Fuentes-Romero, Isamar Moyano-Bravo, Paula Ayala-Garcia, Miguel Angel Rodriguez-Carvajal, Francisco Perez-Montano, Sebastian Acosta-Jurado, Francisco Javier Ollero, Jose-Maria Vinardell

Summary: Rhizobia are soil bacteria that can form nitrogen-fixing symbiosis with legume hosts. This study shows that non-ionic osmotic stress in Sinorhizobium fredii HH103, caused by mannitol, induces changes in gene expression and various bacterial traits, including the production of symbiotic signals.

BIOLOGY-BASEL (2023)

Article Microbiology

Structural Basis of Dual Specificity of Sinorhizobium meliloti Clr, a cAMP and cGMP Receptor Protein

Laura Werel, Neda Farmani, Elizaveta Krol, Javier Serrania, Lars-Oliver Essen, Anke Becker

Summary: In bacteria, the receptor proteins of cAMP and cGMP are found among transcription factors of the Crp-Fnr superfamily. The prototypic Escherichia coli CAP can bind cAMP and cGMP, whereas Sinorhizobium meliloti Clr can activate transcription with both cyclic nucleotides. Crystal structures of Clr-cAMP and Clr-cGMP bound to the DNA binding site reveal similar active conformations induced by the cyclic nucleotides. Clr shows similar affinities for cAMP and cGMP in the presence of DNA, but different affinities in their absence.
Article Multidisciplinary Sciences

DNA-Aeon provides flexible arithmetic coding for constraint adherence and error correction in DNA storage

Marius Welzel, Peter Michael Schwarz, Hannah F. Loechel, Tolganay Kabdullayeva, Sandra Clemens, Anke Becker, Bernd Freisleben, Dominik Heider

Summary: The extensive information capacity of DNA and decreasing costs for DNA synthesis and sequencing make it an attractive alternative to traditional data storage. DNA-Aeon is a concatenated coding scheme for DNA data storage that supports the generation of variable-sized encoded sequences with user-defined constraints.

NATURE COMMUNICATIONS (2023)

Article Genetics & Heredity

Abundance, classification and genetic potential of Thaumarchaeota in metagenomes of European agricultural soils: a meta-analysis

Johanna Nelkner, Liren Huang, Timo W. W. Lin, Alexander Schulz, Benedikt Osterholz, Christian Henke, Jochen Blom, Alfred Puehler, Alexander Sczyrba, Andreas Schlueter

Summary: The family Nitrososphaeraceae is important in agricultural microbiomes. Metabolic reconstruction of Thaumarchaeota MAGs from German soil showed their relevance for soil fertility and plant health. This family plays a crucial role in agricultural soil microbial communities.

ENVIRONMENTAL MICROBIOME (2023)

Article Microbiology

A double-negative feedback loop between NtrBC and a small RNA rewires nitrogen metabolism in legume symbionts

Natalia I. Garcia-Tomsig, Fernando M. Garcia-Rodriguez, Sabina K. Guedes-Garcia, Vicenta Millan, Anke Becker, Marta Robledo, Jose I. Jimenez-Zurdo

Summary: The N status transduced by the NtrBC system is an important signaling cue in the root nodule endosymbiosis, and the sRNA NfeR1 influences the symbiotic performance of Sinorhizobium meliloti by fine-tuning NtrBC output.
Article Microbiology

Uncovering Microbiome Adaptations in a Full-Scale Biogas Plant: Insights from MAG-Centric Metagenomics and Metaproteomics

Julia Hassa, Tom Jonas Tubbesing, Irena Maus, Robert Heyer, Dirk Benndorf, Mathias Effenberger, Christian Henke, Benedikt Osterholz, Michael Beckstette, Alfred Puehler, Alexander Sczyrba, Andreas Schlueter

Summary: This study used metagenomic and metaproteomic analyses to investigate the microbial communities in a full-scale biogas plant with three differently operated digesters. The results showed that these microbial communities had a significant impact on the abundance of enzymes and genes involved in the biogas process, and identified key microorganisms involved in different steps of anaerobic digestion. Further analysis of the highly abundant MAGs in all digesters indicated their potential in ensuring biogas process stability.

MICROORGANISMS (2023)

暂无数据