Article
Agricultural Engineering
Yan Zhang, Yu-ao Su, Xuyang Qiu, Qiuyan Mao, Hongbo Liu, He Liu, Donghui Wen, Zhiguo Su
Summary: This study investigated the variations of class 1 integrons and the ARGs carried by class 1 integrons in anaerobic sludge digesters under different temperatures. The results showed that thermophilic anaerobic digestion was more effective in controlling ARGs carried by class 1 integrons. Moreover, the abundance of class 1 integrons and the length of gene cassette arrays also varied under different temperatures.
BIORESOURCE TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Wenwen Zhu, Tong Wang, Yu Zhu, Linlin Xiao, Weiwei Liu, Quhao Wei
Summary: Integrons are important genetic elements in spreading antibiotic resistance genes, and a 2D-PCR technology has been developed to detect three major integrons simultaneously with low detection limits. This rapid, economic, and high-throughput method is suitable for large-scale integron screening and typing in clinical isolates.
Letter
Microbiology
Paul H. Roy, Sally R. Partridge, Ruth M. Hall
Summary: The article published in Microbiome in July 2018 contains incorrect definitions of integron integrase IntI1 and class 1 integrons, which could affect the interpretation of the data.
Article
Microbiology
Xuefeng Wang, Tian Wang, Mengjiao Guo, Chengcheng Zhang, Zongyi Bo, Yantao Wu, Guoxiang Chao
Summary: This study investigates the molecular mechanism of multidrug resistance (MDR) in Salmonella enterica serovar Indiana (S. Indiana) and the role of large plasmids carrying class 1 integrons in the MDR of foodborne S. Indiana. The results show that S. Indiana isolates carrying class 1 integrons have a significantly higher prevalence of MDR compared to other Salmonella serotypes. The study also reveals the presence of large plasmids carrying class 1 integrons and multiple antimicrobial resistance genes in S. Indiana isolates, and the high conjugation frequency of these integrons. These findings suggest that the horizontal gene transfer of resistance genes facilitated by large plasmids and integrons plays a crucial role in the emergence of MDR clones in S. Indiana.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Environmental Sciences
Cai-Xia Zhao, Xiao-Xuan Su, Mei-Rong Xu, Xin-Li An, Jian-Qiang Su
Summary: The study found integrons associated with antibiotic resistance genes in raw vegetables; cucumber and cabbage had the highest abundance of integron genes; 32 different resistance gene cassettes were detected in the integrons, with most being related to resistance to beta-lactam and aminoglycoside; carrot endophytes had the highest proportion of antibiotic resistance gene cassettes in class 1 integrons.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Microbiology
Niyaz Ali, Yinfu Lin, Ligeng Jiang, Izhar Ali, Ishtiaq Ahmed, Kashif Akhtar, Bing He, Ronghui Wen
Summary: This study investigated the impact of biochar, manure, and their combination on integrons, gene cassettes, and antimicrobial resistance in paddy soil. The results showed that manure increased the abundance and diversity of integrons and antimicrobial resistance, while biochar reduced them.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Genetics & Heredity
Timothy M. Ghaly, Anahit Penesyan, Alexander Pritchard, Qin Qi, Vaheesan Rajabal, Sasha G. Tetu, Michael R. Gillings
Summary: Integrons are genetic elements in microbes that can spread gene cassettes. They are mainly known for spreading antibiotic resistance cassettes among human pathogens. However, gene cassettes encode a wide range of functions important for bacterial adaptation. This study presents reproducible methods for amplifying, processing, and validating gene cassette amplicons from complex communities. The methods can consistently recover thousands of unique cassettes per sample and up to hundreds of different integron integrases. The recovered cassettes confer various functions, including antibiotic resistance.
MICROBIAL GENOMICS
(2022)
Article
Genetics & Heredity
Murielle Baltazar, Nadege Bourgeois-Nicolaos, Macarena Larroude, William Couet, Solange Uwajeneza, Florence Doucet-Populaire, Marie-Cecile Ploy, Sandra Da Re
Summary: Class 1 integrons are genetic elements that play a significant role in the spread of antibiotic resistance. This study shows that the gut is an environment where the class 1 integron integrase is induced and active, highlighting their potential role in acquiring and/or expressing resistance genes, especially during antibiotic therapy.
Article
Biotechnology & Applied Microbiology
S. Kalra, R. Peyser, J. Ho, C. Babbin, N. Bohan, A. Cortes, J. Erley, M. Fatima, J. Flinn, E. Horwitz, R. Hsu, W. Lee, V. Lu, A. Narch, D. Navas, I. Kalu, E. Ouanemalay, S. Ross, F. Sowole, E. Specht, J. Woo, K. Yu, J. D. Coolon
Summary: Precise regulation of transcription is crucial for normal organism form and function, and minor alterations in gene expression can lead to phenotypic variation and disease. In this study, we investigated the response of target genes to systematic reduction of RAP1 expression in Saccharomyces cerevisiae. Our results revealed that Rap1 acts as an activator for more target genes than as a repressor and functions differently in different gene groups. These findings expand our understanding of this important transcriptional regulator.
Article
Food Science & Technology
Fabio Sossai Possebon, Marcus Vinicius Niz Alvarez, Leila Sabrina Ullmann, Joao Pessoa Araujo Jr
Summary: The study evaluated the resistance genes and integrons in multidrug-resistant Salmonella strains isolated from swine mesenteric lymph nodes. The results showed different resistance genes for the same antimicrobial class in each strain with a genetic basis, and the presence of banned olaquindox resistance genes in some samples. Five isolates harbored class 1 integrons, reflecting the high selection pressure these bacterial lineages face along the pork production chain.
Article
Biochemistry & Molecular Biology
Kyle Parcella, Manoj Patel, Yong Tu, Kyle Eastman, Kevin Peese, Eric Gillis, Makonen Belema, Ira B. Dicker, Brian McAuliffe, Bo Ding, Paul Falk, Jean Simmermacher, Dawn D. Parker, Prasanna Sivaprakasam, Javed A. Khan, Kevin Kish, Hal Lewis, Umesh Hanumegowda, Susan Jenkins, John F. Kadow, Mark Krystal, Nicholas A. Meanwell, B. Narasimhulu Naidu
Summary: Allosteric integrase inhibitors (ALLINIs) of HIV-1 have the potential to be a novel mechanism for HIV therapeutics and cure. Modifications to the scaffold structure of ALLINIs have resulted in a series of potent compounds with differentiated ring systems. In particular, inhibitors containing specific core structures showed high antiviral potency and moderate clearance rates in pharmacokinetic studies.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Immunology
William T. Ralvenius, Alison E. Mungenast, Hannah Woolf, Margaret M. Huston, Tyler Z. Gillingham, Stephen K. Godin, Jay Penney, Hugh P. Cam, Fan Gao, Celia G. Fernandez, Barbara Czako, Yaima Lightfoot, William J. Ray, Adrian Beckmann, Alison M. Goate, Edoardo Marcora, Carmen Romero-Molina, Pinar Ayata, Anne Schaefer, Elizabeta Gjoneska, Li-Huei Tsai
Summary: This study uncovers a novel class of anti-inflammatory molecules, represented by A11, which ameliorate neuroinflammation, pathology, and cognitive function in neurodegenerative disorders.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Article
Infectious Diseases
Hanan A. Al-Sarawi, Nazima Habibi, Saif Uddin, Awadhesh N. Jha, Mohammed A. Al-Sarawi, Brett P. Lyons
Summary: In this study, the prevalence of antibiotic-resistance genes (ARGs) in Escherichia coli isolated from mollusks and coastal water samples in Kuwait's marine environment was investigated using a genome-based approach. The study also examined the plasmids and integron genes associated with the spread of ARGs. The findings revealed the presence of various genes conferring resistance to different classes of antibiotics, including those commonly found in E. coli plasmids. Additionally, a significant correlation was observed between integron genes and antibiotic resistance. These results are valuable for monitoring horizontal gene transfer of antibiotic resistance in Kuwait's marine environments.
Article
Microbiology
Teolincacihuatl Ayala Nunez, Gabriela N. Cerbino, Maria Florencia Rapisardi, Cecilia Quiroga, Daniela Centron
Summary: In this study, we evaluated the occurrence of integrase genes, integron systems, and their genetic surroundings in the genus Shewanella. We identified 22 integrase gene types, including 17 newly described ones, indicating multiple lateral genetic transfer events. Our findings suggest that integrase genes and mobile integrons may contribute to the development of multidrug resistance in Shewanella strains.
Article
Environmental Sciences
Ikuro Kasuga, Kyoka Nagasawa, Masato Suzuki, Futoshi Kurisu, Hiroaki Furumai
Summary: Antimicrobial resistance (AMR) is a serious public health concern. This study used high-throughput quantitative polymerase chain reaction (HT-qPCR) to characterize the pollution profiles of antimicrobial resistance genes (ARGs) and mobile gene elements (MGEs) in urban rivers in Tokyo and its surrounding area. The abundance of class 1 integron-integrase gene (intI1) and the array of class 1 integron gene cassettes were also determined. The results showed that effluent discharge from wastewater treatment plants increased the abundance of ARGs and MGEs, and intI1 could be used as a proxy for monitoring these genes in urban rivers.
FRONTIERS IN ENVIRONMENTAL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Julia Bos, Luis H. Cisneros, Didier Mazel
Summary: Membrane vesicles are common carriers of molecular information and primarily disperse along bacterial surfaces. When bacteria are challenged with low doses of antibiotics, vesicle production and movement are significantly enhanced, independent of cell clustering but associated with a reduction in surface appendage density.
Article
Biochemistry & Molecular Biology
Florian Fournes, Theophile Niault, Jakub Czarnecki, Alvise Tissier-Visconti, Didier Mazel, Marie-Eve Val
Summary: Vibrio cholerae, the pathogenic bacterium responsible for cholera, possesses two chromosomes and utilizes the molecule RctB to regulate the coordinated replication process. The study reveals that the crtS site on Chr1 plays a crucial role in controlling the initiation of Chr2 replication, with the extreme C-terminal tail of RctB also being essential for this process. This unique domain in RctB, conserved in all Vibrio species, is likely responsible for the coordinated replication of both chromosomes.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Genetics & Heredity
Andre Carvalho, Didier Mazel, Zeynep Baharoglu
Summary: The study shows that Vibrio cholerae becomes more tolerant to aminoglycosides when VchM is deleted, likely due to increased synthesis of molecular chaperones.
Article
Cell Biology
Andre Carvalho, Evelyne Krin, Chloe Korlowski, Didier Mazel, Zeynep Baharoglu
Summary: Research shows that antibiotics can function as signaling molecules even at subMIC doses, impacting gene expression and stress responses in bacteria. Through transcriptomic and genetic approaches, it was found that quorum-sensing proficiency in Vibrio cholerae plays a significant role in the pathogen's response to the aminoglycoside tobramycin.
Editorial Material
Multidisciplinary Sciences
Didier Mazel
Summary: This article introduces two previously unknown anti-plasmid systems in bacteria and explains how a cholera-causing bacterium eliminates plasmids.
Article
Infectious Diseases
Ana Paula Streling, Rodrigo Cayo, Thais A. Catan, Thomas Jove, Fernanda F. Santos, Carolina S. Nodari, Blake Hanson, William R. Miller, William Shropshire, An Q. Dinh, Julival Ribeiro, Antonio C. C. Pignatari, Cesar A. Arias, Ana C. Gales
Summary: We characterized the complex surrounding regions of the bla GES-16 gene in a Pseudomonas aeruginosa strain in Brazil. The whole genome sequencing analysis revealed the presence of a novel class I integron, In1992, carrying bla GES-16 and surrounded by complex transposition events. Additionally, the exoU gene, associated with severe infections, was also detected.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Review
Cell Biology
Egill Richard, Baptiste Darracq, Celine Loot, Didier Mazel
Summary: Integrons are powerful recombination systems found in bacteria that play a significant role in antibiotic resistance. They have the ability to integrate various environmental and biological signals to adapt to their host. This review highlights the important interconnectivity between integrons and their hosts and the factors that influence the expression of cassettes and integrase.
Article
Infectious Diseases
Anamaria Babosan, Margaux Gaschet, Anaelle Muggeo, Thomas Jove, David Skurnik, Marie-Cecile Ploy, Christophe de Champs, Fany Reffuveille, Thomas Guillard
Summary: Bacteria within biofilms exposed to sub-MIC concentrations of antibiotics can develop antibiotic resistance. This study shows that sub-MIC levels of aminoglycosides induce the SOS response in E. coli carrying the qnrD gene, leading to genetic rearrangements and increased biofilm formation. The presence of the qnrD-plasmid promotes biofilm production and the acquisition and spread of resistance determinants for other antibiotics.
Article
Biochemistry & Molecular Biology
Alberto Hipolito, Lucia Garcia-Pastor, Paula Blanco, Filipa Trigo da Roza, Nicolas Kieffer, Ester Vergara, Thomas Jove, Julio Alvarez, Jose Antonio Escudero
Summary: This study provides experimental evidence against the existence of riboswitch control for aminoglycoside resistance genes in integrons. The researchers found that the induction of these genes was not relevant to the presence of aminoglycosides, but instead, unrelated antibiotics caused a mild but consistently higher increase in expression levels.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Microbiology
Evelyne Krin, Zeynep Baharoglu, Odile Sismeiro, Hugo Varet, Jean-Yves Coppee, Didier Mazel
Summary: In this study, additional type II and type I Toxin/Antitoxin (TA) systems were identified in the Vibrio cholerae N16961 genome. The functional type II TA system was encoded by the VCA0497-0498 operon, while the novel type I TA system was encoded by the VCA0495 gene and its associated antisense non-coding RNAs. These findings increase the number of TA systems in the genome and highlight their importance in this large cassette array.
RESEARCH IN MICROBIOLOGY
(2023)
Article
Biochemical Research Methods
Rocio Lopez-Igual, Pedro Dorado-Morales, Didier Mazel
ACS SYNTHETIC BIOLOGY
(2023)
Article
Microbiology
Murielle Chauvel, Sophie Bachellier-Bassi, Anne-Marie Guerout, Keunsook K. Lee, Corinne Maufrais, Emmanuelle Permal, Juliana Pipoli Da Fonseca, Sadri Znaidi, Didier Mazel, Carol A. Munro, Christophe d'Enfert, Melanie Legrand
Summary: Despite the complete sequencing of the genome of Candida albicans over two decades ago, there are still over 4300 uncharacterized genes. In this study, a collection of over 2500 overexpression mutants was constructed and evaluated to identify genes with fitness costs when overexpressed. This new resource enables routine large-scale screens under different growth conditions and facilitates the characterization of gene functions in C. albicans.
RESEARCH IN MICROBIOLOGY
(2023)
Article
Microbiology
Leticia Larotonda, Damien Mornico, Varun Khanna, Joaquin Bernal-Bayard, Jean-Marc Ghigo, Marie-Eve Val, Diego Comerci, Didier Mazel, Alfonso Soler-Bistue
Summary: The location of ribosomal protein genes on the bacterial chromosome conditions the evolutionary trajectory of Vibrio cholerae. Moving these genes away from the replication origin reduces growth rate, fitness, and infectivity. No mutation can compensate for the growth defect caused by gene relocation.
Review
Biochemistry & Molecular Biology
Pedro Dorado-Morales, Morgan Lamberioux, Didier Mazel
Summary: In recent years, there has been a notable increase in multidrug-resistant pathogens, which poses a challenge to antibiotic-based medicine. In order to prevent dysbiosis in treated patients, there is a need for novel treatments that can specifically target microorganisms without disturbing other species. Conjugation, a process that transfers DNA from one bacterium to another, has been suggested as a method to modify the microbiome and has gained attention for its potential in gene delivery studies.
MOLECULAR MICROBIOLOGY
(2023)
Review
Microbiology
Manon Lang, Andre Carvalho, Zeynep Baharoglu, Didier Mazel
Summary: This review aims to consolidate existing and recent knowledge about the uptake of aminoglycosides (AGs), explore the connection between AG-dependent bacterial stress and drug uptake, and present new strategies to enhance AG uptake for more efficient antibiotic therapies, with a focus on the connection between sugar transport and AG potentiation.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2023)
