Article
Biotechnology & Applied Microbiology
Dimitrios Skliros, Polyxeni Papazoglou, Danai Gkizi, Eleni Paraskevopoulou, Pantelis Katharios, Dimitrios E. Goumas, Sotirios Tjamos, Emmanouil Flemetakis
Summary: In recent years, the biology and biotechnology of bacteriophages have been extensively studied as a means to find environmentally friendly methods to control phytopathogenic bacteria. The use of bacteriophages in biological control of Pseudomonas syringae pv. tomato (Pst) could be an alternative approach to combat bacterial speck disease in tomato plants. The isolated bacteriophage, Medea1, showed promising results in reducing Pst symptoms in tomato plants and upregulating defense-related genes.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Jun Liu, Xuejiang Zhang, Siyi Deng, Hua Wang, Youfu Zhao
Summary: This study investigates the importance of thiamine and its derivative TPP in the growth, stress resistance, and virulence of the plant pathogen PstDC3000. The results show that the absence of thiamine-related genes leads to reduced growth and virulence, but this can be restored with exogenous thiamine. Furthermore, a mutation in a lipoprotein-related gene affects stress tolerance and colonization. Overall, the study highlights the role of the thiamine biosynthetic pathway in the colonization and infection of PstDC3000.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Ning Zhang, Joyce Gan, Lauren Carneal, Juliana Gonzalez-Tobon, Melanie Filiatrault, Gregory B. Martin
Summary: The helper NLRs Nrc2 and Nrc3 play important roles in the immunity of tomato plants to the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) mediated by the sensor NLR Prf and the Pto kinase. They act additively with Prf/Pto and upstream of MAPK signaling. However, they are not required for programmed cell death triggered by another sensor NLR mediating Pst resistance.
Article
Microbiology
Luis Francisco Marin-Ponce, Catalina Rodriguez-Puerto, Perla Rocha-Loyola, Clemencia M. Rojas
Summary: In addition to targeting the transcription factor NTL9, the effector protein HopD1 of the plant pathogenic bacterium Pst DC3000 was found to interfere with the abundance and cellular dynamics of the immune protein AtNHR2B in dynamic vesicles of plants.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Plant Sciences
Zhangjian Hu, Qiaomei Ma, Christine H. Foyer, Cui Lei, Hyong Woo Choi, Chenfei Zheng, Jianxin Li, Jinhua Zuo, Zhuo Mao, Yuyang Mei, Jingquan Yu, Daniel F. Klessig, Kai Shi
Summary: This study reveals the important role of beta CA3 in plant basal immunity under high CO2 in susceptible crops, which differs from its role in Arabidopsis thaliana. The activation of basal immunity by beta CA3 is regulated transcriptionally by the transcription factor NAC43 and post-translationally by the receptor-like kinase GRACE1, indicating a specific mechanism in different plant species. Additionally, the involvement of cell wall metabolism/integrity in beta CA3-mediated basal immunity under both CO2 conditions suggests potential targets for disease management strategies in changing climate.
Article
Biochemistry & Molecular Biology
Penghuan Rui, Xianchu Yang, Shiqiang Xu, Zhanqi Wang, Xueping Zhou, Lei Jiang, Tong Jiang
Summary: In this study, the researchers identified and characterized a novel zinc finger protein, FvZFP1, in Fragaria vesca. Overexpression of FvZFP1 enhanced plant resistance against tobacco mosaic virus and Pseudomonas syringae pv. tomato DC3000, as well as increased tolerance to salinity and drought stress. Furthermore, FvZFP1 was found to activate salicylic acid and ABA pathways in response to stress.
Article
Plant Sciences
Juan Felipe Alfaro-Quezada, Juan Pablo Martinez, Sebastian Molinett, Miryam Valenzuela, Ivan Montenegro, Ingrid Ramirez, Fernando Dorta, Andrea Avila-Valdes, Emna Gharbi, Mingxi Zhou, Helene Dailly, Muriel Quinet, Stanley Lutts, Michael Seeger
Summary: A study found that resistant rootstocks can increase the tolerance of tomato plants to pathogen infection by increasing the concentration of antioxidant compounds and inducing the production of hydrogen sulfide. This study provides new knowledge about the role of rootstocks in the defense of tomato plants, which is important for the sustainable management of tomato cultivation.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biology
Yengkhom Sophiarani, Supriyo Chakraborty
Summary: This study systematically analyzed the codon usage pattern of Pseudomonas syringae pathovar tomato DC3000 (PsPto), and found a strong bias towards G and C nucleotides at the third codon position in its transcriptomes. The results also suggested that codon usage bias is mainly affected by natural selection, and plasmid B is closer to the nuclear genome based on comparative analysis.
JOURNAL OF BIOSCIENCES
(2022)
Article
Plant Sciences
Hyeran Moon, A-Ram Jeong, Oh-Kyu Kwon, Chang-Jin Park
Summary: Orphan genes are commonly found in genomes, but their functions are largely unknown. In this study, a defense-related orphan gene Xio1 was identified and found to play an important role in rice's defense mechanism, providing a new resource for crop breeding.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
L. Furci, D. Pascual-Pardo, L. Tirot, P. Zhang, A. Hannan Parker, J. Ton
Summary: During the past decade, three independent studies have shown that pathogen- and herbivore-exposed Arabidopsis thaliana produces primed progeny with increased resistance. However, the epi-alleles controlling this heritable induced resistance and the mechanisms by which they prime defense genes remain unknown, and the evolutionary significance of the response requires further investigation.
Article
Biochemical Research Methods
Leonardo Furci, David Pascual-Pardo, Jurriaan Ton
Summary: A non-destructive method using bioluminescent Pst::LUX was developed to quantify and visualize spatial-temporal colonisation by Pst in intact leaves of Arabidopsis and tomato. This method allowed for monitoring of Pst infection dynamics and detection of differences in colonisation between plant genotypes and treatments.
Article
Plant Sciences
Benedetta Orfei, Joel F. Pothier, Linda Fenske, Jochen Blom, Chiaraluce Moretti, Roberto Buonaurio, Theo H. M. Smits
Summary: This study sequenced the complete genome of Pseudomonas syringae pv. tomato strain DAPP-PG 215, which was described as a race 0 strain. The analysis revealed that although the strain is phylogenetically related to race 1 strains, it has a race 1 genetic background but harbors a complete ortholog of the avrPto1 gene.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Multidisciplinary Sciences
Haibi Wang, Amy Smith, Amelia Lovelace, Brian H. Kvitko
Summary: This study identified the genes regulated by AlgU inside the plant host and their relationship with PTI through transcriptomic analysis. The results showed that AlgU plays an important role in the upregulation of genes inside the plant host, with stress response and virulence-associated genes being upregulated dependent on AlgU, while flagellar motility genes are downregulated dependent on AlgU. Under PTI conditions, most AlgU-regulated genes lost their induction/suppression response, and almost all AlgU-regulated functional groups were affected by PTI.
Article
Chemistry, Multidisciplinary
Daniele Schiavi, Rosa Balbi, Stefano Giovagnoli, Emidio Camaioni, Ermelinda Botticella, Francesco Sestili, Giorgio Mariano Balestra
Summary: The study evaluated a novel organic nanostructured pesticide composed of chitosan hydrochloride, cellulose nanocrystals, and starch. The pesticide showed effective inhibition of bacterial growth and had no negative effects when applied on tomato plants. It has the potential to reduce Pst epiphytical survival, decrease disease incidence and severity, and be a sustainable and innovative ally in Pst control strategies.
Article
Plant Sciences
Yuhan Gao, Ze Li, Chenyu Yang, Guangyue Li, Hongmei Zeng, Zhonghai Li, Yi Zhang, Xiufen Yang
Summary: Phytopathogens can manipulate plant hormone signaling to inhibit immune responses by inducing the expression of ZAT18 transcription factor, which represses defense responses by directly inhibiting EDS1 and antagonizing salicylic acid accumulation.
Article
Plant Sciences
Paul Rosas-Santiago, Daniel Lagunas-Gomez, Bronwyn J. Barkla, Rosario Vera-Estrella, Sylvie Lalonde, Alexander Jones, Wolf B. Frommer, Olga Zimmermannova, Hana Sychrova, Omar Pantoja
JOURNAL OF EXPERIMENTAL BOTANY
(2015)
Review
Plant Sciences
Alexander M. Jones
Review
Plant Sciences
Ankit Walia, Rainer Waadt, Alexander M. Jones
ANNUAL REVIEW OF PLANT BIOLOGY, VOL 69
(2018)
Article
Biology
Alexander M. Jones, Jonas A. H. Danielson, Shruti N. ManojKumar, Viviane Lanquar, Guido Grossmann, Wolf B. Frommer
Article
Multidisciplinary Sciences
Annalisa Rizza, Ankit Walia, Bijun Tang, Alexander M. Jones
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2019)
Review
Plant Sciences
Annalisa Rizza, Alexander M. Jones
CURRENT OPINION IN PLANT BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Annalisa Rizza, Bijun Tang, Claire E. Stanley, Guido Grossmann, Markus R. Owen, Leah R. Band, Alexander M. Jones
Summary: The study investigated the distribution of the growth regulatory hormone gibberellin (GA) in plant roots and its impact on cell division and elongation patterns. It found that differentials in biosynthetic enzyme steps and cellular permeability contribute to the endogenous GA gradient, affecting root growth.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Cesar L. Cuevas-Velazquez, Tamara Vellosillo, Karina Guadalupe, H. Broder Schmidt, Feng Yu, David Moses, Jennifer An Brophy, Dante Cosio-Acosta, Alakananda Das, Lingxin Wang, Alexander M. Jones, Alejandra A. Covarrubias, Shahar Sukenik, Jose R. Dinneny
Summary: This study introduces a new FRET biosensor, SED1, developed using Arabidopsis intrinsically disordered AtLEA4-5 protein to monitor osmotic stress induced changes in cells. The biosensor demonstrates high sensitivity to osmolarity changes and shows near-linear responses in various living cells, offering a valuable tool for studying water-associated stress. The research highlights the potential of intrinsically disordered protein regions as environmentally-responsive molecular tools across different organisms.
NATURE COMMUNICATIONS
(2021)
Article
Plant Sciences
Pablo Albertos, Tanja Wlk, Jayne Griffiths, Maria J. Pimenta Lange, Simon J. Unterholzner, Wilfried Rozhon, Theo Lange, Alexander M. Jones, Brigitte Poppenberger
Summary: The bHLH transcription factor CESTA and its homologues BEE are involved in gibberellin catabolism and are regulated by brassinosteroids. These findings provide important insights into the regulation of plant growth and development.
Article
Multidisciplinary Sciences
Poonam Mehra, Bipin K. Pandey, Dalia Melebari, Jason Banda, Nicola Leftley, Valentin Couvreur, James Rowe, Moran Anfang, Hugues De Gernier, Emily Morris, Craig J. Sturrock, Sacha J. Mooney, Ranjan Swarup, Christine Faulkner, Tom Beeckman, Rishikesh P. Bhalerao, Eilon Shani, Alexander M. Jones, Ian C. Dodd, Robert E. Sharp, Ari Sadanandom, Xavier Draye, Malcolm J. Bennett
Summary: The study demonstrates how plant roots adapt their branching patterns to efficiently search for soil water resources under heterogeneous conditions. Xerobranching is regulated by the radial movement of abscisic acid, disrupting intercellular communication to inhibit lateral root branching.
Article
Biochemistry & Molecular Biology
Rui Albuquerque-Martins, Dora Szakonyi, James Rowe, Alexander M. Jones, Paula Duque
Summary: By analyzing transgenic plants expressing fluorescently tagged SR45, we found that SR45, an SR protein, can reduce sensitivity to ABA during early seedling development. Exposure to ABA leads to dephosphorylation of multiple amino acid residues on SR45 and its accumulation through reduced ubiquitination and degradation. Through the study of phosphomutant and phosphomimetic transgenic lines, we demonstrate that ABA-mediated dephosphorylation of a specific residue, T264, is important in antagonizing SR45 ubiquitination and degradation to promote its function as a repressor of seedling ABA sensitivity.
PLANT COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Bo Larsen, Roberto Hofmann, Ines S. Camacho, Richard W. Clarke, J. Clark Lagarias, Alex R. Jones, Alexander M. Jones
Summary: Highlighter is a synthetic, light-gated gene expression system developed for plants. It allows unprecedented spatiotemporal control of target gene expression by guiding differential fluorescent protein expression in neighboring cells using light. The system has been used for optogenetic control over plant immunity and pigment production.
Article
Plant Sciences
James Rowe, Mathieu Grange-Guermente, Marino Exposito-Rodriguez, Rinukshi Wimalasekera, Martin O. Lenz, Kartika N. Shetty, Sean R. Cutler, Alexander M. Jones
Summary: ABACUS2 Forster resonance energy transfer biosensors provide unprecedented insights into the accumulation and depletion of abscisic acid (ABA) in living plants. These biosensors exhibit high affinity, signal-to-noise ratio, and orthogonality, allowing the visualization of endogenous ABA patterns in Arabidopsis thaliana. The study reveals the cellular basis for local and systemic ABA functions, particularly in response to reduced foliar humidity. ABA coordination between phloem and root cells is essential for root growth under low humidity conditions, enabling plants to explore deeper soil for water uptake.
Review
Plant Sciences
Martin Balcerowicz, Kartika N. Shetty, Alexander M. Jones
Summary: Phytohormones play a critical role in regulating plant growth, with their levels and distribution being highly dynamic over space and time. Fluorescent biosensors are ideal tools for tracking these dynamics with high spatiotemporal resolution.
Article
Plant Sciences
Annalisa Rizza, Ankit Walia, Viviane Lanquar, Wolf B. Frommer, Alexander M. Jones