Article
Microbiology
Jun Liu, Xuejiang Zhang, Siyi Deng, Hua Wang, Youfu Zhao
Summary: This study investigates the importance of thiamine and its derivative TPP in the growth, stress resistance, and virulence of the plant pathogen PstDC3000. The results show that the absence of thiamine-related genes leads to reduced growth and virulence, but this can be restored with exogenous thiamine. Furthermore, a mutation in a lipoprotein-related gene affects stress tolerance and colonization. Overall, the study highlights the role of the thiamine biosynthetic pathway in the colonization and infection of PstDC3000.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Ning Zhang, Joyce Gan, Lauren Carneal, Juliana Gonzalez-Tobon, Melanie Filiatrault, Gregory B. Martin
Summary: The helper NLRs Nrc2 and Nrc3 play important roles in the immunity of tomato plants to the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) mediated by the sensor NLR Prf and the Pto kinase. They act additively with Prf/Pto and upstream of MAPK signaling. However, they are not required for programmed cell death triggered by another sensor NLR mediating Pst resistance.
Article
Microbiology
Luis Francisco Marin-Ponce, Catalina Rodriguez-Puerto, Perla Rocha-Loyola, Clemencia M. Rojas
Summary: In addition to targeting the transcription factor NTL9, the effector protein HopD1 of the plant pathogenic bacterium Pst DC3000 was found to interfere with the abundance and cellular dynamics of the immune protein AtNHR2B in dynamic vesicles of plants.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Penghuan Rui, Xianchu Yang, Shiqiang Xu, Zhanqi Wang, Xueping Zhou, Lei Jiang, Tong Jiang
Summary: In this study, the researchers identified and characterized a novel zinc finger protein, FvZFP1, in Fragaria vesca. Overexpression of FvZFP1 enhanced plant resistance against tobacco mosaic virus and Pseudomonas syringae pv. tomato DC3000, as well as increased tolerance to salinity and drought stress. Furthermore, FvZFP1 was found to activate salicylic acid and ABA pathways in response to stress.
Article
Plant Sciences
Benedetta Orfei, Joel F. Pothier, Linda Fenske, Jochen Blom, Chiaraluce Moretti, Roberto Buonaurio, Theo H. M. Smits
Summary: This study sequenced the complete genome of Pseudomonas syringae pv. tomato strain DAPP-PG 215, which was described as a race 0 strain. The analysis revealed that although the strain is phylogenetically related to race 1 strains, it has a race 1 genetic background but harbors a complete ortholog of the avrPto1 gene.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Juan Felipe Alfaro-Quezada, Juan Pablo Martinez, Sebastian Molinett, Miryam Valenzuela, Ivan Montenegro, Ingrid Ramirez, Fernando Dorta, Andrea Avila-Valdes, Emna Gharbi, Mingxi Zhou, Helene Dailly, Muriel Quinet, Stanley Lutts, Michael Seeger
Summary: A study found that resistant rootstocks can increase the tolerance of tomato plants to pathogen infection by increasing the concentration of antioxidant compounds and inducing the production of hydrogen sulfide. This study provides new knowledge about the role of rootstocks in the defense of tomato plants, which is important for the sustainable management of tomato cultivation.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biotechnology & Applied Microbiology
Dimitrios Skliros, Polyxeni Papazoglou, Danai Gkizi, Eleni Paraskevopoulou, Pantelis Katharios, Dimitrios E. Goumas, Sotirios Tjamos, Emmanouil Flemetakis
Summary: In recent years, the biology and biotechnology of bacteriophages have been extensively studied as a means to find environmentally friendly methods to control phytopathogenic bacteria. The use of bacteriophages in biological control of Pseudomonas syringae pv. tomato (Pst) could be an alternative approach to combat bacterial speck disease in tomato plants. The isolated bacteriophage, Medea1, showed promising results in reducing Pst symptoms in tomato plants and upregulating defense-related genes.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Zhangjian Hu, Qiaomei Ma, Christine H. Foyer, Cui Lei, Hyong Woo Choi, Chenfei Zheng, Jianxin Li, Jinhua Zuo, Zhuo Mao, Yuyang Mei, Jingquan Yu, Daniel F. Klessig, Kai Shi
Summary: This study reveals the important role of beta CA3 in plant basal immunity under high CO2 in susceptible crops, which differs from its role in Arabidopsis thaliana. The activation of basal immunity by beta CA3 is regulated transcriptionally by the transcription factor NAC43 and post-translationally by the receptor-like kinase GRACE1, indicating a specific mechanism in different plant species. Additionally, the involvement of cell wall metabolism/integrity in beta CA3-mediated basal immunity under both CO2 conditions suggests potential targets for disease management strategies in changing climate.
Article
Plant Sciences
Hyeran Moon, A-Ram Jeong, Oh-Kyu Kwon, Chang-Jin Park
Summary: Orphan genes are commonly found in genomes, but their functions are largely unknown. In this study, a defense-related orphan gene Xio1 was identified and found to play an important role in rice's defense mechanism, providing a new resource for crop breeding.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biology
Yengkhom Sophiarani, Supriyo Chakraborty
Summary: This study systematically analyzed the codon usage pattern of Pseudomonas syringae pathovar tomato DC3000 (PsPto), and found a strong bias towards G and C nucleotides at the third codon position in its transcriptomes. The results also suggested that codon usage bias is mainly affected by natural selection, and plasmid B is closer to the nuclear genome based on comparative analysis.
JOURNAL OF BIOSCIENCES
(2022)
Article
Plant Sciences
L. Furci, D. Pascual-Pardo, L. Tirot, P. Zhang, A. Hannan Parker, J. Ton
Summary: During the past decade, three independent studies have shown that pathogen- and herbivore-exposed Arabidopsis thaliana produces primed progeny with increased resistance. However, the epi-alleles controlling this heritable induced resistance and the mechanisms by which they prime defense genes remain unknown, and the evolutionary significance of the response requires further investigation.
Article
Biochemical Research Methods
Leonardo Furci, David Pascual-Pardo, Jurriaan Ton
Summary: A non-destructive method using bioluminescent Pst::LUX was developed to quantify and visualize spatial-temporal colonisation by Pst in intact leaves of Arabidopsis and tomato. This method allowed for monitoring of Pst infection dynamics and detection of differences in colonisation between plant genotypes and treatments.
Article
Biochemistry & Molecular Biology
Rizwana Begum Syed Nabi, Nkulu Kabange Rolly, Rupesh Tayade, Murtaza Khan, Muhammad Shahid, Byung-Wook Yun
Summary: The study showed that the atbzip62 mutant line lacking the AtbZIP62 gene exhibited enhanced resistance phenotype against Pst DC3000 vir, suggesting that AtbZIP62 may act as a negative regulator of basal defense and systemic acquired resistance in plants under pathogenic bacterial infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Saray Santamaria-Hernando, Alvaro Lopez-Maroto, Clara Galvez-Roldan, Marti Munar-Palmer, Elizabet Monteagudo-Cascales, Jose-Juan Rodriguez-Herva, Tino Krell, Emilia Lopez-Solanilla
Summary: This study demonstrates that the perception of GABA and l-Pro by the PsPto-PscC chemoreceptor drives the entry of Pseudomonas syringae pv. tomato into the tomato apoplast. The recognition of these compounds leads to chemoattraction and is involved in the regulation of GABA catabolism. Mutation of the PsPto-PscC chemoreceptor reduces chemotactic response, impairing entry and reducing virulence in tomato plants. Interestingly, GABA and l-Pro levels increase upon pathogen infection and are involved in the regulation of the plant defense response.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Multidisciplinary Sciences
Haibi Wang, Amy Smith, Amelia Lovelace, Brian H. Kvitko
Summary: This study identified the genes regulated by AlgU inside the plant host and their relationship with PTI through transcriptomic analysis. The results showed that AlgU plays an important role in the upregulation of genes inside the plant host, with stress response and virulence-associated genes being upregulated dependent on AlgU, while flagellar motility genes are downregulated dependent on AlgU. Under PTI conditions, most AlgU-regulated genes lost their induction/suppression response, and almost all AlgU-regulated functional groups were affected by PTI.