Article
Virology
Lotta J. Happonen, Maria I. Pajunen, Jin Woo Jun, Mikael Skurnik
Summary: The study characterizes a T5-type bacteriophage phi R2-01 that infects Yersinia enterocolitica strains, identifying the host receptor and providing insights for potential phage therapy applications in the future.
Article
Biochemistry & Molecular Biology
Jens A. Hammerl, Andrea Barac, Philipp Erben, Julius Fuhrmann, Ashish Gadicherla, Franziska Kumsteller, Anne Lauckner, Felix Mueller, Stefan Hertwig
Summary: Researchers isolated and characterized two phages from game animals, which showed a broad host range and effectively lysed various serotypes of Yersinia enterocolitica. These phages also demonstrated lytic activity against different strains of Yersinia pseudotuberculosis under specific conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jens Andre Hammerl, Sabrin El-Mustapha, Michelle Boelcke, Hannah Trampert, Andrea Barac, Claudia Jaeckel, Ashish K. Gadicherla, Stefan Hertwig
Summary: The study identified three groups of temperate phages in pathogenic Y. enterocolitica strains, each with different lytic activity against different strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Jens A. Hammerl, Andrea Barac, Claudia Jaeckel, Julius Fuhrmann, Ashish Gadicherla, Stefan Hertwig
Summary: Phage vB_YenS_P400 is a virulent siphovirus of Y. enterocolitica with a narrow host range and specific growth conditions, providing insight into the diversity of Yersinia phages.
Article
Infectious Diseases
Phat Tran, Jonathan Kopel, Joe A. Fralick, Ted W. Reid
Summary: This study found that covalently attached selenium to peptides and phage can target and kill Yersinia pestis, without affecting other bacteria.
Article
Biotechnology & Applied Microbiology
Karolina Filik, Bozena Szermer-Olearnik, Joanna Niedziolka-Jonson, Ewa Rozniecka, Jaroslaw Ciekot, Anna Pyra, Irwin Matyjaszczyk, Mikael Skurnik, Ewa Brzozowska
Summary: Yersiniosis is an infectious zoonotic disease caused by two enteropathogenic species of Yersinia bacteria. It is usually transmitted to humans through contaminated food. Reliable diagnosis of Yersiniosis is difficult, but phage adhesins may be a promising diagnostic tool.
Review
Microbiology
Aisylu Shaidullina, Alexander Harms
Summary: Bacteriophages are important predators in ecosystems with potential applications in biotechnology and bacterial infection treatment. Advances in sequencing and genome-wide screens allow for systematic study of phage-host interactions, paving the way for future applications and research expansion.
CURRENT OPINION IN MICROBIOLOGY
(2022)
Article
Microbiology
Dongyang Gao, Hongyue Ji, Xin Li, Xiquan Ke, Xiangmin Li, Pin Chen, Ping Qian
Summary: In this study, a polyvalent broad-spectrum phage, GSP044, was isolated from pig farm sewage, which showed the ability to simultaneously lyse multiple serotypes of Salmonella and E. coli. GSP044 has a short latent period, high stability, and good tolerance to chloroform. Genome sequencing analysis revealed that GSP044 belongs to the Demerecviridae family, Epseptimavirus genus, and does not contain any lysogenicity-related, virulence-related, or antibiotic resistance-related genes. The initial application of phage GSP044 showed promising results in reducing biofilm formation, degrading mature biofilm, and decreasing the number of colonized S. Enteritidis in vitro and in vivo.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Cell Biology
Chengying Ma, Chengkun Wang, Dingyi Luo, Lu Yan, Wenxian Yang, Ningning Li, Ning Gao
Summary: Lateral segregation of membrane constituents into functional microdomains, known as lipid rafts, is a universal principle for cellular membranes. SPFH proteins may play a scaffolding role and regulate specific processes on the membrane. The structure of a supramolecular complex involving SPFH proteins and a membrane-anchored protease has been reported, providing mechanistic insights into their role in membrane organization and functionalization.
Article
Microbiology
Benjamin Sellner, Ruta Prakapaite, Margo van Berkum, Matthias Heinemann, Alexander Harms, Urs Jenal
Summary: Bacteriophage N4 uses a novel surface glycan as a receptor to infect E. coli, with the process regulated by the second messenger c-di-GMP and specific enzymes DgcJ and PdeL. The protection mechanism mediated by PdeL involves reducing c-di-GMP and repressing the wec operon, which serves as a receptor for N4.
Article
Microbiology
Yan D. Niu, Hui Liu, Hechao Du, Ruiqiang Meng, El Sayed Mahmoud, Guihua Wang, Tim A. McAllister, Kim Stanford
Summary: In this study, the efficacy of different bacteriophages against E. coli O157 was evaluated, revealing that the combination of phages did not always follow the individual phage activity. Factors such as temperature, exposure time, and phage strains influenced the interactions among phages, with some combinations showing facilitation while others demonstrated antagonistic effects. The development of phage cocktails should consider confirming lack of antagonism and preferably facilitation or synergistic effects among constituent phages.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Yoshio Nakano, Yusaku Domon, Kenji Yamagishi
Summary: This study used genome-wide comparisons of bacterial species based on their K-mer profiles to construct phylogenetic trees. The results showed that pentanucleotide frequency analysis is an effective method for distinguishing highly similar species. In addition, the study introduced a new R application called Phy5, which generates phylogenetic trees based on pentamer profiles.
Article
Food Science & Technology
Valerie Koller, Diana Seinige, Julia Saathoff, Corinna Kehrenberg, Carsten Krischek
Summary: The combined treatment of UV-C irradiation and PAA spray significantly reduced the levels of Yersinia enterocolitica and Brochothrix thermosphacta on pork samples, extending the shelf-life of meat.
Article
Biology
Signe Christensen, Sebastian Raemisch, Ingemar Andre
Summary: Chaperones play a crucial role in cellular quality control by removing misfolded and aggregated proteins. The chaperone DnaK responds to molecular stress by recognizing hydrophobic regions of misfolded proteins. This study found that the level of DnaK response is correlated to protein stability when overexpressing recombinant proteins. Additionally, stable proteins showed variability in protein abundance and DnaK response among cells.
COMMUNICATIONS BIOLOGY
(2022)
Article
Veterinary Sciences
Liming Jiang, Yaxian Jiang, Wen Liu, Rui Zheng, Chenghua Li
Summary: Escherichia coli, a gram-negative bacterium, is widely distributed and poses a threat to human and animal health. This study identified two phages with good antibiofilm effects against E. coli, which can be potential candidates for phage therapy.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Microbiology
Reina Rau, Andrew J. Darwin
JOURNAL OF BACTERIOLOGY
(2015)
Article
Biochemistry & Molecular Biology
Josue Flores-Kim, Andrew J. Darwin
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Article
Microbiology
Andrei Rajkovic, Sarah Erickson, Anne Witzky, Owen E. Branson, Jin Seo, Philip R. Gafken, Michael A. Frietas, Julian P. Whitelegge, Kym F. Faull, William Navarre, Andrew J. Darwin, Michael Ibba
Review
Microbiology
Josue Flores-Kim, Andrew J. Darwin
ANNUAL REVIEW OF MICROBIOLOGY, VOL 70
(2016)
Article
Microbiology
Josue Flores-Kim, Andrew J. Darwin
JOURNAL OF BACTERIOLOGY
(2016)
Article
Microbiology
Disha Srivastava, Amal Moumene, Josue Flores-Kim, Andrew J. Darwin
Article
Microbiology
Disha Srivastava, Jin Seo, Binayak Rimal, Sung Joon Kim, Stephanie Zhen, Andrew J. Darwin
Article
Immunology
Jin Seo, Andrew J. Darwin
INFECTION AND IMMUNITY
(2013)
Article
Microbiology
Josue Flores-Kim, Andrew J. Darwin
JOURNAL OF BACTERIOLOGY
(2012)
Review
Microbiology
Saori Yamaguchi, Andrew J. Darwin
JOURNAL OF MICROBIOLOGY
(2012)
Article
Biochemistry & Molecular Biology
N. Kaye Horstman, Andrew J. Darwin
MOLECULAR MICROBIOLOGY
(2012)
Article
Biochemistry & Molecular Biology
Saori Yamaguchi, Dylan A. Reid, Eli Rothenberg, Andrew J. Darwin
MOLECULAR MICROBIOLOGY
(2013)
Review
Immunology
Josue Flores-Kim, Andrew J. Darwin
Article
Microbiology
Andrew J. Darwin
Article
Microbiology
Sammi Chung, Andrew J. Darwin
JOURNAL OF BACTERIOLOGY
(2020)