Article
Microbiology
Malligarjunan Rajavel, Vijay Kumar, Ha Nguyen, Jacob Wyatt, Steven H. Marshall, Krisztina M. Papp-Wallace, Prasad Deshpande, Satish Bhavsar, Ravindra Yeole, Sachin Bhagwat, Mahesh Patel, Robert A. Bonomo, Focco van den Akker
Summary: The study on the inhibition of multidrug-resistant pathogens by a new compound and the resistance mechanisms of antibiotics may offer insights into designing novel antibiotics to combat resistant strains.
Article
Chemistry, Multidisciplinary
Soo Khim Chan, Zhongchao Zhao, Samuel Penziner, Ethan Khong, David Pride, Robert T. Schooley, Nicole F. Steinmetz
Summary: Antimicrobial resistance is a global health threat exacerbated by the misuse of antibiotics in medicine and agriculture. Phage therapy, as an alternative antimicrobial treatment, involves using bacteriophages to destroy bacterial pathogens. This study aimed to develop versatile tools for tracking and imaging phages by screening and isolating peptides that bind to specific phages.
Article
Ecology
Lydia-Ann J. Ghuneim, Ruma Raghuvanshi, Kerri A. Neugebauer, Douglas V. Guzior, Martin H. Christian, Bella Schena, Jeremiah M. Feiner, Alicia Castillo-Bahena, Jenna Mielke, Marc McClelland, Douglas Conrad, Isaac Klapper, Tianyu Zhang, Robert A. Quinn
Summary: This study investigates the effect of antibiotics on host-associated microbial communities using cultured sputum samples from cystic fibrosis patients. The researchers found that in addition to the predicted outcomes, antibiotic treatment can also lead to shifts in community composition and an increase in total bacterial load. A mathematical model reveals that the increase in bacterial load is due to the antibiotic-mediated release of pathogens by anaerobic fermentation. Metagenomic sequencing of clinical samples further confirms these findings.
Article
Cell Biology
Wenzhen Lin, Xiaohan Zhu, Li Gao, Mengying Mao, Daming Gao, Zhengwei Huang
Summary: OMD plays a crucial role in osteogenesis by enhancing BMP2-induced osteogenic differentiation and may have potential therapeutic effects in bone defect healing.
CELL DEATH & DISEASE
(2021)
Article
Microbiology
Adam Kawalek, Krzysztof Glabski, Aneta Agnieszka Bartosik, Dominika Wozniak, Magdalena Kusiak, Jan Gawor, Karolina Zuchniewicz, Grazyna Jagura-Burdzy
Summary: This study investigated the interactions of Pseudomonas aeruginosa ParB with other proteins and found that the interaction between ParB and ParA is crucial for chromosome segregation. It also revealed the interactions between ParB and other DNA-binding proteins, metabolic enzymes, and NTPases. These findings suggest an important regulatory role of ParB in the chromosome partitioning system of P. aeruginosa.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Zhongyou Li, Katja Koeppen, Victoria I. Holden, Samuel L. Neff, Liviu Cengher, Elora G. Demers, Dallas L. Mould, Bruce A. Stanton, Thomas H. Hampton
Summary: Researchers developed an algorithm called GAUGE, which automatically annotates GEO microbial data sets, increasing the percentage of analyzable data sets from 4% to 33%. The annotations provide valuable insights and facilitate the identification of consistent patterns of differential gene expression. Additionally, they created a web interface called GAPE for reanalyzing P. aeruginosa and E. coli transcriptomic data.
Article
Biochemistry & Molecular Biology
Raquel Lopez-Galvez, Isabelle Fleurot, Pablo Chamero, Sascha Trapp, Michel Olivier, Claire Chevaleyre, Celine Barc, Mickael Riou, Christelle Rossignol, Antoine Guillon, Mustapha Si-Tahar, Tobias May, Pascal Barbry, Andrea Bahr, Nikolai Klymiuk, Jean-Claude Sirard, Ignacio Caballero
Summary: Research using a pig model demonstrated that stimulation of the TLR5 receptor by flagellin can modulate NF-kappa B signaling and intracellular calcium homeostasis, reducing inflammation in inflammatory lung diseases. This finding suggests flagellin treatment may help regulate the inflammatory response in diseases like cystic fibrosis.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2021)
Article
Plant Sciences
Jieyu Wang, Chong Chen, Chuanzhao Wu, Qingwei Meng, Kunyang Zhuang, Nana Ma
Summary: High-temperature stress is a significant abiotic factor that hinders plant growth and crop yield. This study identified a MYB transcription factor, SlMYB41, in tomatoes that positively regulates tomato thermotolerance by activating the expression of SlHSP90.3.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Microbiology
Liliana Lopez-Pliego, Norarizbeth Lara-Flores, Dalia Molina-Romero, Gabriela May-Compan, Ricardo Carreno-Lopez, Cinthia E. Nunez, Miguel Castaneda
Summary: Azotobacter vinelandii is a motile bacterium with an unusual flagellation pattern. Its motility is primarily regulated by FlhDC, and other negative regulators include AlgU and CydR. The GacS/A-Rsm pathway positively regulates the motility of A. vinelandii, specifically the synthesis of flagella.
CURRENT MICROBIOLOGY
(2022)
Article
Immunology
Sujatha Subramoni, Muhammad Zulfadhly Bin Mohammad Muzaki, Sean C. M. Booth, Staffan Kjelleberg, Scott A. Rice
Summary: The study utilized a mixed-species biofilm community, revealing that Pseudomonas aeruginosa, despite its low abundance, plays a critical role in the community's function, and that it affects interspecific interactions by modulating biofilm structure and physiology.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yuma Shisaka, Erika Sakakibara, Kazuto Suzuki, Joshua Kyle Stanfield, Hiroki Onoda, Garyo Ueda, Miu Hatano, Hiroshi Sugimoto, Osami Shoji
Summary: A stable complex between HasA and TPP was reported, which can inhibit the growth of Pseudomonas aeruginosa and mutant variants of HasA binding FeTPP show a different coordination mode that allows the cyclopropanation of styrene.
Article
Biochemistry & Molecular Biology
Jiraphan Premsuriya, Khedidja Mosbahi, Iva Atanaskovic, Colin Kleanthous, Daniel Walker
Summary: Copper-responsive soluble chaperone proteins are one of the pathways for the two novel pyocins SX1 and SX2 in P. aeruginosa. These proteins have the potential to be used as therapeutics against multi-drug resistant strains.
BIOCHEMICAL JOURNAL
(2023)
Article
Multidisciplinary Sciences
Derek C. K. Chan, Inokentijs Josts, Kalinka Koteva, Gerard D. Wright, Henning Tidow, Lori L. Burrows
Summary: The outer membrane of gram-negative bacteria acts as a barrier against antibiotics, limiting their effectiveness. However, certain antimicrobials can exploit iron transporters to cross this barrier. In this study, we found that the transporter protein FoxA in Pseudomonas aeruginosa can transport the xenosiderophore bisucaberin, in addition to the antibiotic thiocillin. By analyzing the crystal structure, we discovered that bisucaberin forms a unique 3:2 siderophore-iron complex. Mutations in a specific extracellular loop of FoxA affected its binding, uptake, and signal transduction abilities for nocardamine, thiocillin, and bisucaberin. These findings highlight the importance of extracellular loops in controlling ligand uptake and have implications for the development of siderophore-antibiotic conjugates.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Plant Sciences
Yunmin Xu, Yujie Zhu, Xinrui Wang, Zezhou Hong, Aiyi Yang, Yuanyuan Liu, Ling Yan, Yong He, Zhujun Zhu, Huasen Wang
Summary: This study investigates the molecular mechanism regulating rapid elongation of cucumber tendrils. It reveals that CsPRE4 functions as a conserved activator of cell expansion to promote tendril elongation through a HLH-HLH-bHLH-EXPA pathway. This pathway involves the release of CsBEE1, which activates expansin A12 to loosen the cell wall structure in tendrils.
Article
Microbiology
Miriam Rico-Jimenez, Amalia Roca, Tino Krell, Miguel A. Matilla
Summary: In this study, we found that a plant-associated pseudomonas putida strain, which metabolizes IAA, exhibits chemotaxis to IAA independently of auxin metabolism. We discovered that this IAA chemotaxis is mediated by the PcpI chemoreceptor and heterologous expression of pcpI confers IAA taxis to other environmental and human pathogenic isolates of the pseudomonas genus. Our findings suggest that PcpI recognizes and mediates chemoattractions to various aromatic compounds, including salicylic acid, and expands the range of signal molecules recognized by chemoreceptors.
ENVIRONMENTAL MICROBIOLOGY
(2022)