Article
Microbiology
Alexander Seupt, Monika Schniederjans, Juergen Tomasch, Susanne Haeussler
Summary: Elevated mexXY gene expression levels in clinical Pseudomonas aeruginosa isolates result in slight increases in aminoglycoside MIC levels, but rarely lead to clinically relevant resistance phenotypes. The main driver of resistance in these clinical isolates is the acquisition of aminoglycoside-modifying enzymes (AMEs), which is strongly associated with mexY overexpression. The introduction of a gentamicin acetyltransferase confers full resistance levels in a P. aeruginosa type strain only if the MexXY efflux pump is active.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Chemistry, Multidisciplinary
Qian Zhang, Hao-Tian Chi, Linrui Wu, Zixin Deng, Yi Yu
Summary: The biosynthesis of apramycin involves phosphorylation, deacetylation, and dephosphorylation steps with the enzymes AprU, AprP, and AprZ. Streptomyces tenebrarius uses C-5 phosphorylation and N-7' acetylation to avoid auto-toxicity of apramycin. This study provides insights for designing new generation AGAs to combat drug-resistant pathogens.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Microbiology
Emily Bordeleau, Peter J. Stogios, Elena Evdokimova, Kalinka Koteva, Alexei Savchenko, Gerard D. Wright
Summary: Apramycin, an aminoglycoside antibiotic, has a unique structure and resistance mechanism, making it a promising candidate in combating multidrug-resistant pathogens.
Article
Infectious Diseases
Pedro Guirado, Elisenda Miro, Yaidelis Iglesias-Torrens, Ferran Navarro, Susana Campoy, Tyler Scott Alioto, Jessica Gomez-Garrido, Cristina Madrid, Carlos Balsalobre
Summary: This study describes the presence of a plasmid and its transferability in a multi-drug resistant strain of Campylobacter jejuni. The plasmid not only carries tetracycline resistance genes, but also a gene cluster that provides resistance to kanamycin and gentamycin. Additionally, the study suggests the possibility of genetic exchange between Campylobacter and distantly related G-positive bacterial genera.
Article
Microbiology
Yue-tao Chen, Yan-mei Ma, Xuan-xian Peng, Hui Li
Summary: With the wide use of antibiotics, gentamicin-resistant Escherichia coli is causing increasing infections. This study found that glutamine can restore the killing efficacy of gentamicin and expands the range of metabolites contributing to the bactericidal efficiency of aminoglycosides.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Ming Jiang, Xia Li, Chun-Lin Xie, Peng Chen, Wei Luo, Chu-xiao Lin, Qiao Wang, Ding-Ming Shu, Cheng-long Luo, Hao Qu, Jian Ji
Summary: Salmonella enterica is a food-borne pathogen that poses a severe threat to both poultry production and human health. Understanding antibiotic resistance mechanisms and developing novel control measures are essential in combating antibiotic-resistant S. enterica.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Chemistry, Analytical
Luma Clarindo Lopes, Dhesmon Lima, Muhammad Hayat, Yanqi Li, Ayush Kumar, Sabine Kuss
Summary: The emergence and spread of antibiotic resistance is a major challenge to public health. This study presents an electrochemical method for quantifying the retention of tobramycin in susceptible and resistant strains of Pseudomonas aeruginosa, which can distinguish between the two types of strains and identify varying degrees of resistance.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Said M. Daboor, Renee Raudonis, Zhenyu Cheng
Summary: Biofilm infections are difficult to manage with conventional antibiotic treatments due to biofilm structures. The combination treatment of alginate lyase AlyP1400 and tobramycin enhances biofilm cell release, with increased gene expression and antibiotic resistance in dispersed cells, providing insights for more effective therapeutic strategies.
Article
Infectious Diseases
Cuifang Tian, Mengqi Yuan, Qian Tao, Tianming Xu, Jing Liu, Zhenhua Huang, Qian Wu, Yingjie Pan, Yong Zhao, Zhaohuan Zhang
Summary: Improper use of antibiotics leads to the emergence of antibiotic-resistant strains and renders the treatment of infectious diseases ineffective. This study demonstrates that Vibrio parahaemolyticus develops biofilm defense mechanisms in response to aminoglycoside antibiotics, significantly impacting their resistance to these antibiotics.
Article
Microbiology
Riti Mann, Rayane Rafei, Cindy Gunawan, Christopher J. Harmer, Mohammad Hamidian
Summary: Carbapenem resistance in Acinetobacter baumannii is primarily due to the global spread of two main clones that carry oxa23, oxa24, and oxa58. However, new carbapenem-resistant clones are emerging that are also resistant to a wide range of antibiotics.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Clemence Cuzin, Pamela Houee, Pierrick Lucas, Yannick Blanchard, Christophe Soumet, Arnaud Bridier
Summary: The study evaluated the potential of PHMB to select antibiotic resistance in bacteria, finding that exposure to PHMB led to the emergence of a variant of E. coli with stable resistance to gentamicin, associated with a nonsense mutation in the aceE gene. This demonstrated the potential for enzymes of central metabolic pathways to be a target for adaptation strategies, leading to cross-resistance towards biocides and antibiotics in bacteria.
Article
Microbiology
Mohamad Maatouk, Ahmad Ibrahim, Jean-Marc Rolain, Vicky Merhej, Fadi Bittar
Summary: Candidate Phyla Radiation (CPR) microbes have been identified as a new branch in the tree of life, representing over 26% of microbial diversity. Despite their small size, reduced genomes, and reliance on symbiotic relationships with bacterial hosts, CPR microbes are abundant in various environments. They possess a diverse range of antibiotic resistance genes and are active participants in microbial competition, equipped with mechanisms for combat in different habitats.
Article
Biochemistry & Molecular Biology
Hafizur Rahman, Chika Fukushima, Hidetaka Kaya, Takashi Yaeno, Kappei Kobayashi
Summary: This study explores the potential use of NtMAR1hs as a recessive selective marker for DNA-free genome editing using CRISPR/Cas9-sgRNA ribonucleoprotein complex. The knockout of NtMAR1hs in tobacco plants demonstrated their role in sensitivity to aminoglycoside antibiotics, similar to Arabidopsis MAR1. The study also suggests the presence of additional functional homologs in tobacco.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Jessica D. Podoll, Emma Rosen, Wei Wang, Yuefeng Gao, Jing Zhang, Xiang Wang
Summary: Novel antibacterial agents are urgently needed to combat antibiotic resistance. Researchers have discovered a small molecule that can make methicillin-resistant Staphylococcus aureus sensitive to beta-lactam antibiotics again, with low potential for resistance development. This molecule, known as trypyricins, belongs to a new class of broad-spectrum antibacterials and shows promise as an antibiotic adjuvant in fighting resistant bacteria.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Pharmacology & Pharmacy
Nuoyan Wang, Xuemin Chen, Jing Luo, Fei Deng, Fuguo Shi, Qin Wu, Yasi Huang, Qin Ouyang, Rongxin Qin, Hong Zhou
Summary: This study investigated the antibacterial sensitization effect of DHA27 combined with tobramycin in tobramycin-resistant Pseudomonas aeruginosa. The combination showed significant antibacterial effects on many resistant strains and reduced bacterial load in the spleen and lungs in a mouse model. The study also revealed that the effect was related to the inhibition of mRNA expression of aminoglycoside-modifying enzymes.
FRONTIERS IN PHARMACOLOGY
(2022)