Article
Biology
Lucas M. Walker, James R. J. Haycocks, Julia C. Van Kessel, Triana N. Dalia, Ankur B. Dalia, David C. Grainger
Summary: Many bacteria use quorum sensing to control changes in lifestyle. In Vibrio cholerae, quorum-sensing signals are transduced to the transcription factor LuxO. HapR targets 32 loci and overlaps with sites for the cAMP receptor protein (CRP), allowing integration of quorum sensing and cAMP signaling to control gene expression.
Article
Microbiology
Courtney N. Dial, Steven J. Eichinger, Randi Foxall, Christopher J. Corcoran, Alice H. Tischler, Robert M. Bolz, Cheryl A. Whistler, Karen L. Visick
Summary: Bacterial motility is crucial for the symbiotic colonization of the squid Euprymna scolopes by Vibrio fischeri, with strain KB2B1 exhibiting a unique migration pattern compared to ES114, possibly due to mutations in the quorum sensing pathway and DGC gene. The mutations shifted KB2B1 to a perceived low cell density state and inhibited migration, highlighting differences in regulatory control between strains.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Jojo A. Prentice, Andrew A. Bridges, Bonnie L. Bassler
Summary: This study investigates how quorum sensing and c-di-GMP signaling jointly regulate biofilm morphogenesis in bacteria, showing a surprising synergy between these two major sensory inputs in controlling biofilm development.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Microbiology
Matthew Jemielita, Ameya A. Mashruwala, Julie S. Valastyan, Ned S. Wingreen, Bonnie L. Bassler
Summary: Bacteria utilize quorum sensing to orchestrate collective behaviors, with Vibrio cholerae forming multicellular communities. This aggregation process consists of two subprograms - void formation and motility-dependent entry. The timing of aggregation is controlled by four extracellular proteases, ensuring reliable community formation.
Article
Microbiology
Yiquan Zhang, Yue Qiu, He Gao, Junfang Sun, Xue Li, Miaomiao Zhang, Xingfan Xue, Wenhui Yang, Bin Ni, Lingfei Hu, Zhe Yin, Renfei Lu, Dongsheng Zhou
Summary: The master QS regulator OpaR in Vibrio parahaemolyticus directly regulates a group of genes to impact the concentration of c-di-GMP, which in turn influences behaviors such as biofilm formation. This direct association between QS and c-di-GMP metabolism allows for precise control of gene transcription and bacterial behaviors.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Environmental Sciences
April C. Armes, Alison Buchan
Summary: The study provides genetic evidence that the two QS systems in Rhodobacterales sp. Y4I work hierarchically to coordinate the production of the antimicrobial indigoidine and biofilm formation. The QS2 system, pgaRI, is shown to be at the top of the regulatory hierarchy governing indigoidine biosynthesis, while QS1 system, phaRI, influences biofilm development. Additionally, c-di-GMP levels are altered in QS and indigoidine biosynthesis Y4I mutants, indicating its integration into the QS circuitry of this strain.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Fuwen Liu, Fei Wang, Yixuan Yuan, Xiaoran Li, Xiaojun Zhong, Menghua Yang
Summary: Vibrio parahaemolyticus is a significant food-borne pathogen. The study showed that the three signal synthases, CqsA(vp), LuxM(vp), and LuxS(vp), are essential for activating quorum sensing and regulating swarming in V. parahaemolyticus. The absence of these synthases leads to swarming defects.
MOLECULAR MICROBIOLOGY
(2023)
Article
Immunology
Yuehua Li, Junxiang Yan, Jinghao Li, Xinke Xue, Ying Wang, Boyang Cao
Summary: This study identified a new quorum sensing regulator, LuxT, which directly inhibits hapR expression, leading to increased virulence in Vibrio cholerae. It was also found that the expression of LuxT is regulated by the two-component system ArcB/ArcA. These findings reveal a novel cell density-mediated virulence regulation pathway and contribute to a better understanding of the pathogenic mechanisms of V. cholerae.
Article
Biochemistry & Molecular Biology
Rotem Sela, Brian K. Hammer, Malka Halpern
Summary: The study found that interactions between Vibrio cholerae and chironomid egg mass microbiota can affect HAP production and egg mass degradation activities, potentially providing insights into the interactions between this pathogen and human gut microbiota.
Editorial Material
Biochemistry & Molecular Biology
Leonie Unterholzner
Summary: The DNA sensor cGAS and its adaptor STING form an ancient pathogen detection mechanism, but its conservation across the animal kingdom is uncertain. In this article in Cell, Kranzusch and colleagues identify numerous cGAS-like receptors and uncover networks of second messengers that activate innate immune responses in animals.
Article
Multidisciplinary Sciences
Dayna C. Patterson, Myrrh Perez Ruiz, Hyerin Yoon, Johnnie A. Walker, Jean-Paul Armache, Neela H. Yennawar, Emily E. Weinert
Summary: Cyclic dimeric guanosine monophosphate (c-di-GMP) acts as a second messenger in bacteria, modulating processes like biofilm formation. A globin-coupled sensor protein (DcpG) was found to have bifunctional c-di-GMP enzymatic activity, with different regulation of GGDEF and EAL domains by gas binding to the heme. DcpG's ability to control enzymatic activity in response to ligand binding is due to the unique properties of the globin domain.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Microbiology
Jennifer K. Teschler, Carey D. Nadell, Knut Drescher, Fitnat H. Yildiz
Summary: Biofilms are a growth mode in which microbial communities are structured and embedded in a polymeric extracellular matrix. In this study, the biofilm formation of Vibrio cholerae is focused and the current understanding of its formation, including attachment, matrix components, dynamics, regulation, and dispersal, is summarized. The decision to form or disperse from biofilms is regulated by a complex network that integrates various environmental inputs.
ANNUAL REVIEW OF MICROBIOLOGY
(2022)
Article
Microbiology
Jessica G. Tague, Abish Regmi, Gwendolyn J. Gregory, E. Fidelma Boyd
Summary: Factor for inversion stimulation (Fis) positively regulates the expression of qrr sRNAs in V. parahaemolyticus, controlling swarming motility indirectly through the QS pathway and directly through the surface sensing pathway.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hema P. Narra, Abha Sahni, Krishna Mohan Sepuru, Jessica Alsing, Sanjeev K. Sahni
Summary: This study identified a putative diguanylate cyclase PleD in the genomes of pathogenic rickettsiae, responsible for the synthesis of c-di-GMP. It was found that levels of pleD changed dynamically after infection with R. rickettsii (Sheila Smith) in vitro, while there was no such change with R. rickettsii (Iowa). These findings provide the first evidence for the potentially important role(s) of c-di-GMP in host-cell responses to pathogenic rickettsiae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Courtney N. Dial, Lauren Speare, Garrett C. Sharpe, Scott M. Gifford, Alecia N. Septer, Karen L. Visick
Summary: Vibrio fischeri efficiently colonizes its squid host by producing a transient biofilm dependent on symbiosis polysaccharide. Yeast extract inhibits biofilm formation, while omitting it and adding vitamin pABA and calcium can induce biofilm formation. pABA increases the concentration of c-di-GMP, which plays a crucial role in inducing biofilm formation.
Review
Infectious Diseases
Rebecca C. Harris, Louis Grandjean, Laura J. Martin, Alexander J. P. Miller, Joseph-Egre N. Nkang, Victoria Allen, Mishal S. Khan, Katherine Fielding, David A. J. Moore
BMC INFECTIOUS DISEASES
(2016)
Review
Infectious Diseases
Rebecca C. Harris, Mishal S. Khan, Laura J. Martin, Victoria Allen, David A. J. Moore, Katherine Fielding, Louis Grandjean
BMC INFECTIOUS DISEASES
(2016)
Article
Medicine, General & Internal
Rebecca C. Harris, Peter J. Dodd, Richard G. White
Review
Biotechnology & Applied Microbiology
Rebecca C. Harris, Tom Sumner, Gwenan M. Knight, Richard G. White
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2016)
Review
Infectious Diseases
Charles R. Beck, Bruce C. McKenzie, Ahmed B. Hashim, Rebecca C. Harris, Arina Zanuzdana, Gabriel Agboado, Elizabeth Orton, Laura Bechard-Evans, Gemma Morgan, Charlotte Stevenson, Rachel Weston, Mitsuru Mukaigawara, Joanne Enstone, Glenda Augustine, Mobasher Butt, Sophie Kim, Richard Puleston, Girija Dabke, Robert Howard, Julie O'Boyle, Mary O'Brien, Lauren Ahyow, Helene Denness, Siobhan Farmer, Jose Figureroa, Paul Fisher, Felix Greaves, Munib Haroon, Sophie Haroon, Caroline Hird, Rachel Isba, David A. Ishola, Marko Kerac, Vivienne Parish, Jonathan Roberts, Julia Rosser, Sarah Theaker, Dean Wallace, Neil Wigglesworth, Liz Lingard, Yana Vinogradova, Hiroshi Horiuchi, Javier Penalver, Jonathan S. Nguyen-Van-Tam
INFLUENZA AND OTHER RESPIRATORY VIRUSES
(2013)
Review
Infectious Diseases
Gayle P. Dolan, Rebecca C. Harris, Mandy Clarkson, Rachel Sokal, Gemma Morgan, Mitsuru Mukaigawara, Hiroshi Horiuchi, Rachel Hale, Laura Stormont, Laura Bechard-Evans, Yi-Sheng Chao, Sergey Eremin, Sara Martins, John Tam, Javier Penalver, Arina Zanuzadana, Jonathan S. Nguyen-Van-Tam
INFLUENZA AND OTHER RESPIRATORY VIRUSES
(2013)
Review
Immunology
Charles R. Beck, Bruce C. McKenzie, Ahmed B. Hashim, Rebecca C. Harris, Jonathan S. Nguyen-Van-Tam
JOURNAL OF INFECTIOUS DISEASES
(2012)
Article
Multidisciplinary Sciences
Tendesayi Kufa, Tonderai Mabuto, Evans Muchiri, Salome Charalambous, Dominique Rosillon, Gavin Churchyard, Rebecca C. Harris
Article
Public, Environmental & Occupational Health
Rebecca C. Harris, Tom Sumner, Gwenan M. Knight, Tom Evans, Vicky Cardenas, Chen Chen, Richard G. White
LANCET GLOBAL HEALTH
(2019)
Article
Public, Environmental & Occupational Health
Partho Roy, Johan Vekemans, Andrew Clark, Colin Sanderson, Rebecca C. Harris, Richard G. White
LANCET GLOBAL HEALTH
(2019)
Review
Immunology
Aaron S. Karat, Meghann Gregg, Hannah E. Barton, Maria Calderon, Jayne Ellis, Jane Falconer, Indira Govender, Rebecca C. Harris, Mpho Tlali, David Aj Moore, Katherine L. Fielding
Summary: The study found that implementing multiple infection prevention and control measures in healthcare facilities can reduce the transmission of Mycobacterium tuberculosis, but evidence for the direct effectiveness of triage, isolation, or effective treatment measures is limited and of low quality.
CLINICAL INFECTIOUS DISEASES
(2021)
Letter
Respiratory System
C. Finn McQuaid, Nicky McCreesh, Jonathan M. Read, Tom Sumner, Rein M. G. J. Houben, Richard G. White, Rebecca C. Harris
EUROPEAN RESPIRATORY JOURNAL
(2020)
Review
Medicine, General & Internal
C. K. Weerasuriya, R. A. Clark, R. G. White, R. C. Harris
JOURNAL OF INTERNAL MEDICINE
(2020)
Article
Immunology
Gayle P. Dolan, Rebecca C. Harris, Mandy Clarkson, Rachel Sokal, Gemma Morgan, Mitsuru Mukaigawara, Hiroshi Horiuchi, Rachel Hale, Laura Stormont, Laura Bechard-Evans, Yi-Sheng Chao, Sergey Eremin, Sara Martins, John S. Tam, Javier Penalver, Anna Zanuzadana, Jonathan S. Nguyen-Van-Tam
EMERGING INFECTIOUS DISEASES
(2012)