Article
Biochemistry & Molecular Biology
Sirikul Ngawiset, Abbas Ismail, Shuichiro Murakami, Piamsook Pongsawasdi, Thanyada Rungrotmongkol, Kuakarun Krusong
Summary: This study aimed to identify the functional amino acid positions of CgAM involved in LR-CD synthesis through site-directed mutagenesis and molecular dynamic simulation. Several mutants were constructed, purified, and characterized, and their effects on LR-CD production were evaluated. The results showed that specific amino acid mutations affected different enzymatic activities and LR-CD yields, providing insights for future modifications to enhance LR-CD properties and production.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Bhavna Maurya, Melwin Colaco, Johan Wouters, Lionel Pochet, Sandra Misquith
Summary: Homoserine-acetyltransferases, serine-acetyltransferases, and homoserine-succinyltransferases all belong to the MetX family and have evolved from a common ancestor by conserving the catalytic site and substrate binding residues. The substrate selection differs due to substrate-specific residues lining the substrate-binding pocket.
Article
Multidisciplinary Sciences
Jana Skerlova, Jens Berndtsson, Hendrik Nolte, Martin Ott, Pal Stenmark
Summary: The pyruvate dehydrogenase complex (PDHc) is a multienzyme complex that converts pyruvate into acetyl-coenzyme A, linking glycolysis to the citric acid cycle. The core of PDHc is formed by dihydrolipoyl transacetylase, which provides binding sites for other enzymes and shuffles reaction intermediates between the active sites through covalently bound lipoyl domains. The cryo-EM structure of the E. coli dihydrolipoyl transacetylase core in a resting state reveals molecular details of the assembly and interactions at the active site.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Ayano Komine-Abe, Naoko Kondo, Shosei Kubo, Hisashi Kawasaki, Makoto Nishiyama, Saori Kosono
Summary: In Corynebacterium glutamicum, acetylation of the lysine residue at position 391 on CgE2 was found to play a critical role in the assembly of PDH and ODH complexes, affecting their activities. Substitution of Lys391 with arginine in the CgE2-K391R variant strain led to increased l-glutamate production and reduced pyruvate accumulation, suggesting potential advantages for enhancing production efficiency.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Ning Li, Weizhu Zeng, Jingwen Zhou, Sha Xu
Summary: This study reports for the first time the efficient production of OAH using C. glutamicum as the chassis, laying a solid foundation for industrial production of OAH.
BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
(2022)
Article
Biochemistry & Molecular Biology
Katharina Raasch, Edith Malecki, Maria Siemann, Malayko M. Martinez, Juergen J. Heinisch, Janine Mueller, Lidia Bakota, Christian Kaltschmidt, Barbara Kaltschmidt, Helmut Rosemeyer, Roland Brandt
CHEMICAL BIOLOGY & DRUG DESIGN
(2015)
Article
Biochemistry & Molecular Biology
Sabine Krawczyk, Katharina Raasch, Christian Schultz, Melanie Hoffelder, Lothar Eggeling, Michael Bott
Article
Biotechnology & Applied Microbiology
Katharina Raasch, Marco Bocola, Joerg Labahn, Alexander Leitner, Lothar Eggeling, Michael Bott
JOURNAL OF BIOTECHNOLOGY
(2014)
Article
Neurosciences
Elinor Lazarov, Merle Hillebrand, Simone Schroeder, Katharina Ternka, Julia Hofhuis, Andreas Ohlenbusch, Alonso Barrantes-Freer, Luis A. Pardo, Marlene U. Fruergaard, Poul Nissen, Knut Brockmann, Jutta Gaertner, Hendrik Rosewich
NEUROBIOLOGY OF DISEASE
(2020)
Article
Multidisciplinary Sciences
Matthias Kettwig, Katharina Ternka, Kristin Wendland, Dennis Manfred Krueger, Silvia Zampar, Charlotte Schob, Jonas Franz, Abhishek Aich, Anne Winkler, M. Sadman Sakib, Lalit Kaurani, Robert Epple, Hauke B. Werner, Samy Hakroush, Julia Kitz, Marco Prinz, Eva Bartok, Gunther Hartmann, Simone Schroeder, Peter Rehling, Marco Henneke, Susann Boretius, A. Alia, Oliver Wirths, Andre Fischer, Christine Stadelmann, Stefan Nessler, Jutta Gaertner
Summary: Infantile-onset RNaseT2 deficient leukoencephalopathy is characterized by cystic brain lesions, white matter alterations, cerebral atrophy, and psychomotor impairment. Studies using Rnaset2(-/-) mice reveal upregulation of interferon-stimulated genes, IFNAR1-dependent neuroinflammation, and insights into hippocampal-accentuated brain atrophy and cognitive impairment. This study provides a potential animal model for investigating CNS damage associated with RNaseT2 deficiency and exploring therapeutic interventions for interferon-driven brain pathology.
NATURE COMMUNICATIONS
(2021)