Article
Microbiology
Olga Karaseva, Georgii Ozhegov, Dilyara Khusnutdinova, Maria Siniagina, Elizaveta Anisimova, Farida Akhatova, Rawil Fakhrullin, Dina Yarullina
Summary: Lactiplantibacillus plantarum is a versatile bacterium widely used as a probiotic. In this study, the probiotic potential of a novel strain called L. plantarum FCa3L, isolated from fermented cabbage, was characterized through genome sequencing. The results showed that FCa3L had comparable properties to the reference strain, L. plantarum 8PA3, in terms of acid and bile tolerance, adhesiveness, H2O2 production, and acidification rate. FCa3L also exhibited superior antibacterial properties, although it had a higher antibiotic resistance gene content. The study provided genomic evidence supporting the adhesive and antibacterial properties, biosynthesis of bioactive metabolites, and safety of FCa3L, suggesting its potential as a probiotic.
Article
Agriculture, Dairy & Animal Science
Shujuan Yang, Chenglin Deng, Yao Li, Weicheng Li, Qiong Wu, Zhihong Sun, Zhenhui Cao, Qiuye Lin
Summary: In this study, a potential probiotic strain L. plantarum ST was isolated from De'ang pickled tea in Yunnan Province, China, and its genome was fully sequenced and analyzed. The results showed that L. plantarum ST exhibited strong tolerance and antibacterial activity, indicating its potential application in animal breeding.
JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Young Joon Oh, Jieun Lee, Seul Ki Lim, Min-Sung Kwon, Sulhee Lee, Sang-Pil Choi, Dohyeon Yu, Yeon-su Oh, Jinho Park, Hak-Jong Choi
Summary: Lactobacillus johnsonii 7409N31 was isolated from the feces of a healthy Hanwoo calf and its genome was completely sequenced. The strain possesses genes encoding enzymes involved in carbohydrate hydrolysis. These findings provide a theoretical basis for developing probiotic feed additives that improve nutrient digestibility.
JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Sendeku Takele Alemneh, Shimelis Admassu Emire, Bernd Hitzmann
Summary: This study evaluated the suitability of a teff-based substrate for developing probiotic functional beverages, finding that both Lactobacillus rhamnosus GG (LGG) and Lactobacillus plantarum A6 (LA6) could grow and produce desired products in the substrate. The combination of the two evaluated lactobacilli strains reduced fermentation time, indicating the potential for producing functional probiotic beverages using teff flour as a substrate without any supplements.
Article
Biotechnology & Applied Microbiology
Hongbiao Li, Ting Liu, Xiuqin Zhang, Zhiguo Xiong, Qing Hong, Shuang Jia, Yi Lin, Liping Wang, Yueliang Zhao
Summary: This study used genomic tools to characterize the probiotic and bactericidal potential of Lactiplantibacillus plantarum HY41. It was found that HY41 carried multiple functional genes and a putative bacteriocin biosynthetic cluster. A silent gene cluster responsible for producing a potent bacteriocin was also identified in HY41. The mode of action of the bacteriocin was studied and it was found to cause cell death by disrupting the cell membrane of Listeria monocytogenes. These findings confirm the probiotic and bactericidal properties of HY41 and demonstrate its potential for industrial probiotic applications.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2023)
Article
Immunology
Lihong Zhang, Hongcai Ma, Muhammed Fakhar-E-Alam Kulyar, Huachun Pan, Kewei Li, Aoyun Li, Quan Mo, Yaping Wang, Hailong Dong, Yuhua Bao, Jiakui Li
Summary: This study analyzed the probiotic characteristics and potential probiotic ability of L. fermentum YLF016. The strain exhibited high survival rate, antibacterial activity, antioxidant activity, and strong adherence to intestinal cells, indicating its potential as a probiotic.
MICROBIAL PATHOGENESIS
(2022)
Article
Food Science & Technology
Shucen Feng, Haitao Wang, Xinping Lin, Huipeng Liang, Sufang Zhang, Yingxi Chen, Chaofan Ji
Summary: The increasing demand for healthy food has sparked interest in developing new food products containing probiotics. This study assessed the potential of probiotics and prebiotics to create novel gummies. The results confirmed the safety of 25 strains of Lactobacillus plantarum derived from fermented foods, showing their tolerance to simulated gastrointestinal fluids. Moreover, mushroom polysaccharides were found to have a proliferative effect on potential probiotics and protected their survival in the gastrointestinal fluids. These findings demonstrate the promising application of probiotics and prebiotics in the form of gummies as a probiotic food.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Mengwei Wang, Yang Li, Jie Yang, Rui Shi, Liu Xiong, Qingjie Sun
Summary: This study investigated the adsorption amounts and antibacterial effects of inorganic nanoparticles on probiotic bacteria. Results showed that food-grade NPs have bactericidal effects on L. plantarum and L. fermentum.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Fengxuan Lang, Jianzhu Wen, Zhen Wu, Daodong Pan, Linjun Wang
Summary: Lactic acid bacteria fermentation is a simple and safe method of food preservation. Using probiotics in yoghurt can improve the flavor, texture, and health benefits of dairy products. This study found that the mixed culture condition enhanced the texture properties of yoghurt, and L. plantarum A3 fermented yoghurt accumulated additional components that led to significant sensory differences. These results suggest that L. plantarum A3 is a potential probiotic strain that enhances the sensory and nutritional properties of fermented milk.
FOOD SCIENCE AND HUMAN WELLNESS
(2022)
Article
Agriculture, Dairy & Animal Science
Soyeon Park, Jung-Ae Kim, Hyun-Jun Jang, Dae-Hyuk Kim, Yangseon Kim
Summary: Lactobacillus amylovorus CACC736, originated from swine feces in Korea, has a complete genome sequence consisting of one circular chromosome (2,057,809 base pair [bp]) with 38.2% guanine-cytosine (GC) content and two circular plasmids, namely, pCACC736-1 and pCACC736-2. The predicted protein-coding genes involve CRISPR-associated proteins, bacteriocin (helveticin J) biosynthesis, and proteins related to bile acid tolerance. Additionally, genes related to vitamin B-group biosynthesis (riboflavin and cobalamin) were also identified in L. amylovorus CACC736. Overall, the complete genome sequence of L. amylovorus CACC736 will contribute to the development of functional probiotics in the animal industry.
JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Lina Zhao, Qinggang Xie, Fengyi Shi, Shengnan Liang, Qingxue Chen, Smith Etareri Evivie, Ji Qiu, Bailiang Li, Guicheng Huo
Summary: This study aimed to reduce cow milk protein allergenicity by fermenting skim milk protein with Lactobacillus helveticus and Lactobacillus plantarum strains. The fermentation process inhibited antigenicity of specific milk proteins and cleaved major allergic epitopes, demonstrating a potential method for reducing milk sensitization.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Nutrition & Dietetics
Dongting Yang, Wentao Lyu, Ziyi Hu, Jiting Gao, Zhiyao Zheng, Weijun Wang, Jenni Firrman, Daxi Ren
Summary: The study evaluated the probiotic effects of Lactobacillus fermentum ZJUIDS06 and Lactobacillus plantarum ZY08 on hyperlipidemic golden hamsters, demonstrating that Lactobacillus fermentum ZJUIDS06 increased colon SCFA levels, reduced serum LDL, total cholesterol, and triglycerides, and altered the cecum microbiota. Particularly, Lactobacillus fermentum ZJUIDS06 increased the abundance of Parabacteroides, indicating its potential in lowering cholesterol levels.
FRONTIERS IN NUTRITION
(2021)
Article
Food Science & Technology
Erdong Yuan, Miao Zhou, Zhengyang Liang, William Kwame Amakye, Chuanli Hou, Jiaoyan Ren
Summary: Our study aims to provide a framework for the isolation of potential effective fractions from sturgeon protein guided by probiotic adhesion. We found that Fr2, one of the fractions obtained through anion exchange resin column, significantly increased the intestinal adhesion rates of LP45 and LGG. The primary structure of the protein 14.3 kDa in Fr2 was identified using mass spectrometry.
Article
Food Science & Technology
Dong-Mei Liu, Yan-Yan Huang, Ming-Hua Liang
Summary: This study explored the probiotic properties and adaptive responses of Lactiplantibacillus plantarum DMDL 9010(LP9010) isolated from Chinese traditional fermented paocai. LP9010 showed good tolerance to gastrointestinal environment and exhibited various probiotic functions. Comparative genomic analysis identified unique genes and stress-related proteins in LP9010.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Infectious Diseases
Reuven Rasooly, Alex C. Howard, Naomi Balaban, Bradley Hernlem, Emmanouil Apostolidis
Summary: Probiotic bacteria help maintain gut microbiome homeostasis, and witch hazel promotes the growth and survival of probiotics while inhibiting the growth and virulence of pathogenic bacteria.
Article
Chemistry, Applied
Xiaolong Jiang, Jing Bai, Jian Yuan, Huijing Zhang, Gege Lu, Yuhui Wang, Lingyan Jiang, Bin Liu, Lei Wang, Di Huang, Lu Feng
Summary: By reprogramming the metabolic network, the research team increased the yield of OPS-based glycoconjugate vaccines to 38.6 mg L-1, the highest reported so far. Mass spectrometry analysis showed a high glycosylation efficiency of the designed glycosylation sequon in CTB-O5 and CTB-O7, demonstrating the effectiveness of the glyco-optimized platform in producing vaccines against ExPEC.
CARBOHYDRATE POLYMERS
(2021)
Article
Immunology
Shujie Li, Yu Pang, Si Zhang, Qing Li, Qian Wang, Lei Wang, Lu Feng
Summary: This study provides the first comprehensive analysis of the transcriptome profile of UPEC during its intra-BECs life, revealing a new virulence-associated gene and its regulatory mechanism.
MICROBIAL PATHOGENESIS
(2021)
Article
Multidisciplinary Sciences
Lingyan Jiang, Peisheng Wang, Xiaorui Song, Huan Zhang, Shuangshuang Ma, Jingting Wang, Wanwu Li, Runxia Lv, Xiaoqian Liu, Shuai Ma, Jiaqi Yan, Haiyan Zhou, Di Huang, Zhihui Cheng, Chen Yang, Lu Feng, Lei Wang
Summary: S. Typhimurium infection induces upregulated glycolysis and decreased serine synthesis in host macrophages, leading to accumulation of glycolytic intermediates that promote bacterial replication and virulence.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Nan Song, Lihong Chen, Zhemin Zhou, Xingmei Ren, Bo Liu, Siyu Zhou, Caihong Wang, Yun Wu, Nicholas R. Waterfield, Jian Yang, Guowei Yang
Summary: This study revealed that Tc toxins are widely present among Gram-negative and positive bacterial genomes, with a database constructed containing numerous Tc loci and putative toxic domains for potential future pest control systems. The study also highlighted the importance of Tc toxins as tools for biotechnological applications and their potential roles in human infectious diseases.
Article
Biochemical Research Methods
Zhemin Zhou, Jane Charlesworth, Mark Achtman
Summary: pHierCC is a scalable clustering scheme based on core genome multi-locus typing, allowing incremental, static, and multi-level cluster assignments of genomes. HCCeval is used to identify optimal thresholds for assigning genomes to cohesive HierCC clusters. HierCC has genotyped over 530,000 genomes from various bacteria species since its implementation in 2018.
Article
Multidisciplinary Sciences
Yoshan Moodley, Andrea Brunelli, Silvia Ghirotto, Andrey Klyubin, Ayas S. Maady, William Tyne, Zilia Y. Munoz-Ramirez, Zhemin Zhou, Andrea Manica, Bodo Linz, Mark Achtman
Summary: The study reveals the coevolutionary history of Helicobacter pylori with humans pre-dating the out-of-Africa diaspora, as well as the genetic relationships between Siberians and Native Americans. The genetic diversity of bacteria across Eurasia and the Americas is structured into three populations, with Siberian bacteria showing both ancient divergence and recent admixture.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Yutao Liu, Bin Liu, Tingting Xu, Qian Wang, Wendi Li, Jialin Wu, Xiaoyu Zheng, Bin Liu, Ruiying Liu, Xingmei Liu, Xi Guo, Lu Feng, Lei Wang
Summary: The study identifies a fructose uptake system crucial for the growth of Vibrio cholerae in seawater, where fructose concentrations are low. This system may be important for the survival of pandemic V. cholerae in seawater.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Bin Liu, Chengqian Qian, Pan Wu, Xiaodan Li, Yutao Liu, Huiqian Mu, Min Huang, Yang Zhang, Tianyuan Jia, Yuanyuan Wang, Lu Wang, Xiao Zhang, Di Huang, Bin Yang, Lu Feng, Lei Wang
Summary: Enterohemorrhagic Escherichia coli regulates its cell wall structure during infection, balancing survival in the intestinal lumen and infection of intestinal epithelial cells. The bacteria express long-chain OAg in the intestinal lumen for increased resistance to antimicrobial peptides, but reduce OAg chain length upon sensing epithelial cell attachment, leading to enhanced injection of T3SS effectors into epithelial cells for host cell infection. This regulatory mechanism may be widespread in pathogenic bacteria and closely related to bacterial pathogenesis.
Article
Food Science & Technology
Min Wang, Bin Liu, Yanli Xu, Xi Guo, Tian Fu, Jiafei Liu, Lei Wang
Summary: In this study, the O-antigen gene clusters of V. fluvialis were analyzed by whole-genome sequencing, and a microsphere-based suspension array (MSA) was developed for the identification of different serogroups. Additionally, 13 new O-antigen gene cluster types were discovered. This method provides a rapid and efficient tool for detecting and identifying V. fluvialis serogroups, with potential applications in epidemiological investigations and food safety.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Cell Biology
Yu Pang, Zhihui Cheng, Si Zhang, Shujie Li, Xueping Li, Xiaodan Li, Xiao Zhang, Xiaoxiao Li, Yingxing Feng, Heting Cui, Zhen Chen, Le Liu, Qing Li, Jianxiao Huang, Mingqing Zhang, Siwei Zhu, Lei Wang, Lu Feng
Summary: Urinary tract infections are mainly caused by uropathogenic Escherichia coli (UPEC). This study reveals that UPEC senses host immune responses and initiates escape by upregulating a key phospholipase. The findings provide insights into pathogenic bacteria's response to intracellular phosphate shortage and tackling host defense, and offer potential for developing new therapeutic agents to treat UPEC infection.
Article
Cell Biology
Lu Feng, Bin Yang, Yongchang Xu, Yingying Xiong, Fang Wang, Bin Liu, Wen Yang, Ting Yao, Lei Wang
Summary: This study reveals a signal transduction pathway for the activation of virulence in EHEC O157:H7, providing insights into the relationship between adherence, gene regulation, and pathogenicity.
Article
Cell Biology
Hao Sun, Xuehua Wan, Yu Fan, Peng Liu, Yajun Song, Ningyu Zhu, Zhifeng Duan, Qian Wang, Fang Chen, Changhong Zhou, Yangyang Zheng, Peng Ding, Fenxia Liu, Lu Feng, Kwang Sik Kim, Lei Wang
Summary: The immune response of brain cells to invading bacteria and the mechanism used by pathogenic bacteria to escape immune surveillance in the brain are still not well understood. Microglia can engulf pathogenic bacteria and activate astrocytes to produce C3 for antibacterial activity. Pathogenic bacteria can sense low concentration of threonine in cerebrospinal fluid to reduce the immune response.
Article
Biology
Mark Achtman, Zhemin Zhou, Jane Charlesworth, Laura Baxter
Summary: The definition of bacterial species is a taxonomic issue, while bacterial populations are identified by population genetics. However, traditional methods are not adequate for the millions of genomic sequences from bacterial pathogens. EnteroBase's HierCC clustering method can accurately assign genomes to species/subspecies and populations.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2022)
Article
Multidisciplinary Sciences
Bin Liu, Yutao Liu, Bin Yang, Qian Wang, Xingmei Liu, Jingliang Qin, Ke Zhao, Fan Li, Xiaohui Feng, Linxing Li, Pan Wu, Miaomiao Liu, Siwei Zhu, Lu Feng, Lei Wang
Summary: This study reveals that EHEC O157:H7 acquired a previously uncharacterized regulatory system rbfSR, which senses riboflavin produced by gut microbiota and activates the expression of virulence genes. This acquisition represents an essential step in the evolution of the highly pathogenic O157:H7. The introduction of rbfSR into other EHEC serotypes also increased their pathogenicity, indicating a potential public health issue.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Muxiu Jiang, Heng Li, Xiao Liu, Nan Shen, Yuanjie Zhou, Wenting Song, Xing Wang, Qing Cao, Zhemin Zhou
Summary: This study analyzed the genomic features of 198 CRKP isolates and reported the clinical risk, genetic diversity, and antimicrobial resistance prevalence in pediatric patients. The bla(NDM) genes were found to be the most predominant carbapenemase genes, followed by bla(KPC-2) and bla(IMP). The dissemination of carbapenemases was mainly facilitated by four types of plasmids. This research highlights the importance of genome sequencing in monitoring and controlling CRKP infections.
MICROBIOLOGY SPECTRUM
(2023)
