Article
Biotechnology & Applied Microbiology
Z. Wu, R. Zheng, J. Zhang, S. Wu
Summary: This study investigated the anti-biofilm and anti-infection mechanism of EPS273 on a transcriptional level using an RNA-Seq approach. The results showed that EPS273 can reduce biofilm formation and infection of P. aeruginosa by downregulating the expression of the PhoP-PhoQ and QS systems.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Microbiology
Alexandra J. J. Lee, Georgia Doing, Samuel L. L. Neff, Taylor Reiter, Deborah A. A. Hogan, Casey S. S. Greene
Summary: This article studies the genomic heterogeneity of Pseudomonas aeruginosa strains, compares the expression patterns of core genes and accessory genes between PAO1 and PA14 strains, and finds differences in the expression patterns of some core genes among different strains.
Article
Biochemistry & Molecular Biology
Karolina Kotecka, Adam Kawalek, Magdalena Modrzejewska-Balcerek, Jan Gawor, Karolina Zuchniewicz, Robert Gromadka, Aneta Agnieszka Bartosik
Summary: Pseudomonas aeruginosa PA3973 is an uncharacterized transcriptional regulator that influences the survival ability and stress response of the pathogen by controlling the expression of other genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Olga Matatkova, Jana Michailidu, Richard Jezdik, Irena Jarosova Kolouchova, Tomas Rezanka, Vladimir Jirku, Jan Masak
Summary: Study on the optimization of rhamnolipid production by Pseudomonas aeruginosa DBM 3774 using different carbon sources and cultivation strategies showed that the carbon source type, concentration, and C/N ratio had a significant effect on the composition of rhamnolipid congeners.
Article
Biochemistry & Molecular Biology
Leena Putzeys, Maarten Boon, Eveline-Marie Lammens, Konstantin Kuznedelov, Konstantin Severinov, Rob Lavigne
Summary: RNA sequencing is widely used to study the transcription of phage-infected bacteria. However, short-read RNA sequencing methods often fail to capture the boundaries of transcripts, making it challenging to discover key transcription events and operon structures. In this study, ONT-cappable-seq, a long-read RNA sequencing technique, was developed to enable comprehensive analysis of prokaryotic transcripts.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Immunology
Eluzia C. Peres-Emidio, Gustavo J. C. Freitas, Marliete C. Costa, Ludmila Gouveia-Eufrasio, Livia M. V. Silva, Anderson P. N. Santos, Paulo H. F. Carmo, Camila B. Brito, Raquel D. N. Arifa, Rafael W. Bastos, Noelly Q. Ribeiro, Lorena V. N. Oliveira, Monique F. Silva, Tatiane A. Paixao, Alessandra M. Saliba, Caio T. Fagundes, Daniele G. Souza, Daniel A. Santos
Summary: This study investigated the interaction between Cryptococcus and Pseudomonas aeruginosa (Pa), and found that Pa inhibited the growth of Cryptococcus in vitro and enhanced the fungicidal activity of macrophages. In in vivo experiments, previous Pa infection reduced the severity and lethality of cryptococcosis, possibly by decreasing the fungal burden in the lungs and brain and inducing inflammation. The results suggest that Pa plays an important protective role in coinfections.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Kim Thomsen, Niels Hoiby, Peter Ostrup Jensen, Oana Ciofu, Claus Moser
Summary: Biofilm infections are resistant to host responses and antibiotics. The host immune response plays a crucial role in the outcome and exacerbation of chronic infections. In chronic Pseudomonas aeruginosa lung infections, antibody response and Th1/Th2 imbalance have been associated with a worse outcome.
Article
Biochemistry & Molecular Biology
Xiaorui Lou, Runhao Wang, Jiaqi Yan, Weiping Li, Ruihua Liu, Qionglin Zhang, Mark Bartlam
Summary: Chronic pulmonary infections in individuals with cystic fibrosis or chronic obstructive pulmonary disease are promoted by alginate production from opportunistic pathogen Pseudomonas aeruginosa. SrfA is a novel stress response facilitator in P. aeruginosa, activated by cell wall stress, and interacts with high affinity with PA2559 and PA2560. Understanding the three-dimensional structure of SrfA provides insights into its role in response to cell wall stress.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Microbiology
Christopher J. Jones, Nikolas Grotewold, Daniel J. Wozniak, Erin S. Gloag
Summary: Chronic biofilm infections by Pseudomonas aeruginosa have a significant impact on patient morbidity and mortality, and understanding the bacterial response to surface attachment and biofilm formation can provide important insights into the development of biofilms.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Raquel Lopez-Galvez, Isabelle Fleurot, Pablo Chamero, Sascha Trapp, Michel Olivier, Claire Chevaleyre, Celine Barc, Mickael Riou, Christelle Rossignol, Antoine Guillon, Mustapha Si-Tahar, Tobias May, Pascal Barbry, Andrea Bahr, Nikolai Klymiuk, Jean-Claude Sirard, Ignacio Caballero
Summary: Research using a pig model demonstrated that stimulation of the TLR5 receptor by flagellin can modulate NF-kappa B signaling and intracellular calcium homeostasis, reducing inflammation in inflammatory lung diseases. This finding suggests flagellin treatment may help regulate the inflammatory response in diseases like cystic fibrosis.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2021)
Article
Genetics & Heredity
Ruan Fourie, Errol D. Cason, Jacobus Albertyn, Carolina H. Pohl
Summary: The interaction between Candida albicans and Pseudomonas aeruginosa is complex and involves physical interaction and secretion of factors by the bacterium. Transcriptome analysis of early biofilms revealed differential regulation of genes associated with stress, iron and zinc homeostasis, and cell membrane synthesis in C. albicans in response to P. aeruginosa, potentially influencing their commensalism and pathogenesis.
G3-GENES GENOMES GENETICS
(2021)
Article
Genetics & Heredity
Ruan Fourie, Errol D. Cason, Jacobus Albertyn, Carolina H. Pohl
Summary: The interaction between Candida albicans and Pseudomonas aeruginosa in polymicrobial biofilms is complex, with the former exhibiting differential gene regulation in response to hypoxia and stress associated with the presence of the latter. This leads to changes in biofilm formation, morphology, and phenotypic switching, highlighting the unique transcriptional profiles that may influence commensalism and pathogenesis in these biofilms.
G3-GENES GENOMES GENETICS
(2021)
Article
Microbiology
Jun Kwon, Sang Wha Kim, Sang Guen Kim, Jeong Woo Kang, Won Joon Jung, Sung Bin Lee, Young Min Lee, Sib Sankar Giri, Cheng Chi, Se Chang Park
Summary: This study isolated and characterized a novel bacteriophage, pPa_SNUABM_DT01, with strong lytic ability against Pseudomonas aeruginosa. Comparative genomic analysis revealed that this phage may belong to a novel genus. The phage showed potential as an alternative to antibiotics for treating antibiotic-resistant infections.
Article
Chemistry, Multidisciplinary
Louis M. M. Mouterde, Julien Couvreur, Maxime M. J. Langlait, Fanny Brunois, Florent Allais
Summary: The study identified a potentially new cyclohexanone monooxygenase (CHMO) sequence from the complete genome of the Pseudomonas aeruginosa strain Pa1242, which was confirmed through bioconversion to obtain a key precursor 2H-HBO. The novel sequence exhibited CHMO activity and achieved high productivity and total conversion rates through optimization of culture conditions.
Article
Microbiology
Verena Ducret, Diego Gonzalez, Sara Leoni, Martina Valentini, Karl Perron
Summary: The control of cellular zinc concentrations is crucial for the survival and virulence of Pseudomonas aeruginosa. In this study, it was found that the Zur protein, a known repressor of zinc import, also plays a role in zinc export. Zur represses zinc entry in situations of zinc excess and activates the transcription of czcR, a positive regulator of the zinc export system. This discovery highlights a new role of Zur as a global regulator of zinc homeostasis in P. aeruginosa.