Article
Microbiology
Lauren A. Riley, Irenee C. Payne, Melissa Tumen-Velasquez, Adam M. Guss
Summary: This study developed a system for integrating up to 12 genetic constructs into the E. coli chromosome. Site-specific recombinases were used to catalyze recombination between specific DNA sequences, enabling the integration of heterologous DNA. The utility of this system was demonstrated by the insertion of eight DNA methyltransferases into the E. coli chromosome.
JOURNAL OF BACTERIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Ke Liu, Gui-Hong Lin, Kun Liu, Yong-Jun Liu, Xin-Yi Tao, Bei Gao, Ming Zhao, Dong-Zhi Wei, Feng-Qing Wang
Summary: In this study, a multiplexed integrase-assisted site-specific recombination (miSSR) method was developed to integrate genes/pathways in Mycolicibacteria for the purpose of constructing cell factories. The method successfully integrated multiple copies of genes using different bacteriophage systems and a reconstructed Xer recombinase system. The results showed that the miSSR system is an effective and convenient tool for engineering the genomes of Mycolicibacteria and other related strains.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2022)
Article
Microbiology
Gang Zhang, Qinna Cui, Jianjuan Li, Ruiliang Guo, Sebastien Olivier Leclercq, Lifeng Du, Na Tang, Yuqin Song, Chao Wang, Fangqing Zhao, Jie Feng
Summary: In this study, the mobility of GIsul2 and CR2-sul2 units mediated by integrase was experimentally characterized. The results demonstrate that the integrase of GIsul2 can perform the excision and integration of GIsul2 and CR2-sul2 unit by site-specific recombination between att core sites. The findings suggest a potential and unappreciated role of the GIsul2 integrase family in the dissemination of CR2-sul2 units carrying various resistance determinants.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Gang Zhang, Shigang Yao, Yunan Liu, Hailing Fang, Yuqin Song, Chao Wang, Dawei Wei, Jie Feng
Summary: In this study, we identified 530 novel tyrosine-type integrases from bacterial genomic islands. These integrases belong to a distinct evolutionary clade and are affiliated with new integrase groups. The integration functions of six representative novel integrases were validated in a bacterial model system, and their expression patterns were confirmed in Escherichia coli. This study revealed site-specific recombination functions of novel integrases and expands the toolbox for genetic engineering.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Microbiology
Jing Yang, Lina Liu, Yu Feng, Da He, Chengcheng Wang, Zhiyong Zong
Summary: The newly identified mcr-10 plasmid-borne colistin resistance gene was found in a Cronobacter sakazakii isolate. It may be mobilized through site-specific recombination mediated by an integrase of an integrative mobile element.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Kimihiro Abe, Takumi Takahashi, Tsutomu Sato
Summary: This study demonstrates the importance of the extreme C-terminal region (ECT) of SprA in DNA recombination and directionality control in Bacillus subtilis. The interaction between SprA and SprB is influenced by the alanine residue Phe532 in the extreme C-terminal region, affecting the directional control of the recombination reaction.
MOLECULAR MICROBIOLOGY
(2021)
Article
Plant Sciences
Sisi Ge, Xingshui Dong, Yangminghao Liu, Kathryn M. Wright, Sonia N. Humphris, Lionel X. Dupuy, Michael P. MacDonald
Summary: Optical trapping techniques were used to quantify the factors affecting bacterial attachment on living root surfaces and determine the relationship between contact duration and maintenance on the surface. The system successfully guided Pectobacterium atrosepticum (Pba) cells to various root cell types, including root hair cells, epidermal cells, border cells, and laser-ablated tissues. This technique has the potential to enhance our understanding of attachment and biofilm formation mechanisms in the rhizosphere.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Chemistry, Multidisciplinary
Yu Cheng, Da Han, Weihong Tan, Cheng Lv, Yuan Li, Mingzhi Zhang
Summary: Intercellular interactions are important in biological processes and their dysregulation leads to disease progression. Mimicking multicellular behaviors in vitro is technically challenging due to dynamic interactions. In this study, DNA was used as a lock and key to regulate intercellular interactions and improve antitumor therapy efficacy.
Article
Optics
Yuhan Zhong, Xiao Lin, Jing Jiang, Yi Yang, Gui-Geng Liu, Haoran Xue, Tony Low, Hongsheng Chen, Baile Zhang
Summary: The research found that the near-field directionality of a dipolar source can be efficiently toggled by tailoring the polarization of surface waves, providing a feasible scheme for active control. Interestingly, this scheme can transform a circular electric/magnetic dipole into a Huygens dipole in the near-field coupling, and also enable actively flipping the near-field coupling and non-coupling faces of Janus dipoles.
LASER & PHOTONICS REVIEWS
(2021)
Article
Genetics & Heredity
Reine U. Protacio, Mari K. Davidson, Wayne P. Wahls
Summary: Meiosis is a crucial process in the sexual life cycle of eukaryotes, contributing to genetic diversity through independent chromosome assortment and meiotic recombination. Recent studies have shown significant changes in the frequency distribution of recombination between closely related species, subspecies, and isolated populations of the same species. Analysis of recombination plasticity in fission yeast has provided new insights into the mechanisms underlying these rapid evolutionary changes, with different classes of hotspots acting as independently controlled rheostats. This dynamic modulation can rapidly and dramatically alter the global frequency distribution of recombination, contributing to the evolutionarily rapid changes in the recombination landscape.
FRONTIERS IN GENETICS
(2022)
Article
Infectious Diseases
Ya Hao, Da Teng, Ruoyu Mao, Na Yang, Jianhua Wang
Summary: This study obtained a candidate sequence PN7 with high expression, low toxicity, and desirable antibacterial activity through structural optimization and modification. PN7 showed strong activity against several species of Gram-positive bacteria and was relatively stable in various conditions. PN7 may be a novel promising antimicrobial agent, and this study provides a model or an example for the design, modification, or reconstruction of novel AMPs and their derivatives.
Review
Cell Biology
Vikash Kumar Yadav, Corentin Claeys Bouuaert
Summary: The article outlines the latest advances in understanding the mechanism of meiotic DSB formation, emphasizing the tight regulation of DSBs and the significance of recombination mechanisms.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Microbiology
Sridhar Mandali, Reid C. Johnson
Summary: Bacteriophage serine integrases catalyze specific recombination reactions between DNA segments called att sites, with the help of a directionality factor. The integrases include a DNA-binding region with a recombinase domain and a zinc-binding domain, with unique coiled-coil motifs. Mutations in specific residues control the formation of synaptic complexes, with CC motifs playing a crucial role in recombination even after initial assembly. The site-specific recombination reactions promoted by these integrases are essential for the life cycle of bacteriophages and have potential applications in genetic engineering and synthetic biology.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Genetics & Heredity
Shaked Nir Heyman, Mika Golan, Batia Liefshitz, Martin Kupiec
Summary: PCNA acts as a processivity factor and a landing pad for proteins during DNA synthesis. The interaction between PCNA and Pol delta is mediated by PIPs, specifically on Pol32. The weak interaction of the exonuclease mutant pol3-01 with PCNA leads to increased mutagenesis and sister chromatid recombination.
Article
Chemistry, Multidisciplinary
Xiewei Xiong, Mingshu Xiao, Wei Lai, Li Li, Chunhai Fan, Hao Pei
Summary: Precise control of DNA-circuit functions is key for constructing complex DNA networks, and optochemically controlled DNA switching circuits can regulate computation functions, including multiple logic computations and probabilistic computation.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)