Article
Biochemical Research Methods
Transito Garcia-Garcia, Thibaut Douche, Quentin Giai Gianetto, Sandrine Poncet, Nesrine El Omrani, Wiep Klaas Smits, Elodie Cuenot, Mariette Matondo, Isabelle Martin-Verstraete
Summary: This study provides a detailed map of kinase-substrate relationships in C. difficile, revealing the significant role of protein phosphorylation in bacterial cell signaling and pathogenicity. The identified phosphoproteins targeted different pathways involved in bacterial growth and metabolism, shedding light on their regulatory mechanisms.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Microbiology
Weiyi Li, Yifan Yin, Yu Meng, Zhe Ma, Huixing Lin, Hongjie Fan
Summary: In this study, phosphoproteomic analysis identified GlmM as a substrate of STK, with phosphorylation at the Ser-101 site impacting its enzyme activity and cell wall peptidoglycan synthesis. Mutants lacking glmM or with a S101A point mutation showed aberrant cell morphology, attenuated virulence, and reduced enzyme activities and peptidoglycan concentrations. These findings demonstrated that STK phosphorylates GlmM to regulate SS2 pathogenicity.
VETERINARY MICROBIOLOGY
(2021)
Article
Microbiology
Abida Sultan, Carsten Jers, Tariq A. Ganief, Lei Shi, Meriem Senissar, Julie Bonne Kohler, Boris Macek, Ivan Mijakovic
Summary: Understanding phosphorylation-mediated regulation of metabolic enzymes and pathways during metabolic shifts is a major challenge. In this study, proteome and phosphoproteome analysis of E. coli Delta yeaG revealed the phosphorylation of AceA by YeaG during the shift from glucose to malate, suggesting a role in this metabolic transition. SILAC-based approaches have shown utility in identifying kinase substrates under relevant physiological conditions.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Christine R. Zheng, Abhyudai Singh, Alexandra Libby, Pamela A. Silver, Elizabeth A. Libby
Summary: By developing modular, direct, and specific sensors of bacterial kinase activity, researchers successfully measured the activity of PrkC kinase in different growth conditions, and found that its activity increased in response to a cell-wall active antibiotic.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Biology
Xiaoting Wang, Haitao Ge, Ye Zhang, Yingchun Wang, Pengpeng Zhang
Summary: Our study demonstrated that the Ser/Thr protein kinase SpkI plays an important role in maintaining high-effective photosynthesis during high-salt acclimation process in Synechocystis. The mutant Delta spkI showed significantly low maximal quantum yield of PSII under high salt condition, along with slower growth, decreased net photosynthesis, and modified activities of PSI and cytochrome b(6)f complex. These findings suggest that SpkI is crucial for the adaptation of photosynthetic organisms to high salinity.
Article
Biochemistry & Molecular Biology
Richa Virmani, Prashant Pradhan, Jayadev Joshi, Avril Luyang Wang, Hem Chandra Joshi, Andaleeb Sajid, Anoop Singh, Vishal Sharma, Bishwajit Kundu, Daniel Blankenberg, Virginie Molle, Yogendra Singh, Gunjan Arora
Summary: PrkC protein kinase plays a vital role in the phenotypic memory and germination of Bacillus anthracis spores, and the loss of PrkC-dependent phosphorylation affects spore development. The key metabolic enzyme Phosphoglycerate mutase (Pgm) is involved in spore germination by catalyzing the isomerization of 2-PGA and 3-PGA. This study demonstrates that PrkC phosphorylates and regulates Pgm, negatively regulating its activity and maintaining its apo-like isoform before germination.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Microbiology
Xuan Zhu, Chao Feng, Lantian Zhou, Zhenzhen Li, Yue Zhang, Jianyi Pan
Summary: Pseudomonas aeruginosa is a significant human pathogen that urgently requires the development of new antibacterial drugs, as recognized by the WHO. Recent research has revealed the importance of eukaryote-type Ser/Thr protein kinases, such as Stk1, in regulating bacterial physiological processes. The proteomic analysis of a Stk1 knockout mutant strain of P. aeruginosa PAO1 showed significant changes in protein expression, providing insights into the physiological role of Stk1.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yusuke Fujino, Takero Miyagawa, Masayuki Torii, Masao Inoue, Yuki Fujii, Hiroki Okanishi, Yoshikatsu Kanai, Ryoji Masui
Summary: A study found that a thermophilic Ser/Thr protein kinase completely unfolds at high temperatures, but is significantly stabilized by its natural ligands ATP and ADP, increasing the melting temperature to 80 degrees Celsius. This striking effect of a substrate on the thermostability of thermophilic proteins has not been reported before.
Article
Biochemistry & Molecular Biology
Cyrus Ahmadi Toussi, Javad Haddadnia, Cherif F. Matta
Summary: The elastic network model, as a novel tool, shows high accuracy and speed in predicting the activity of molecules with different chemical structures but common biological activity, making it a promising tool for industrial applications such as drug and material design.
MOLECULAR DIVERSITY
(2021)
Article
Biochemical Research Methods
Chen Ye, Yongze Ge, Yue Zhang, Lantian Zhou, Wei Chen, Xuan Zhu, Jianyi Pan
Summary: The marine bacterial pathogen Vibrio parahaemolyticus is a major cause of food-borne gastroenteritis. Recent research has identified VP0057 as a potential Ser/Thr protein kinase with phosphorylation activity, leading to proteome-wide changes in response to its deletion. The deletion of VP0057 resulted in differential expression of 197 proteins, notably upregulating iron-related and T6SS1-related proteins and promoting Fe2+ and Fe3+ uptake.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Engineering, Chemical
Megumi Ikeura, Hiroto Tashiro, Yuka Yamagata, Hikaru Saito, Tamaki Kobayashi, Masataka Mizunuma, Kazuki Yamazaki, Keisuke Baba, Kazuhiro Furukawa, Yoshiro Chuman
Summary: In this study, we found an antibody-like small molecule protein adnectin, named PMDB-1, that specifically binds to PPM1D. By inhibiting the activity of PPM1D and stabilizing p53, PMDB-1 showed inhibition of cell proliferation in breast cancer cells.
Article
Microbiology
Transito Garcia-Garcia, Sandrine Poncet, Elodie Cuenot, Thibaut Douche, Quentin Giai Gianetto, Johann Peltier, Pascal Courtin, Marie-Pierre Chapot-Chartier, Mariette Matondo, Bruno Dupuy, Thomas Candela, Isabelle Martin-Verstraete
Summary: The STK signaling pathway regulates PGH homeostasis to precisely control PG hydrolysis during cell division. The study on PG hydrolase CwlA in C. difficile reveals its important role in cell division and its regulation by PrkC, highlighting a novel regulatory mechanism by which STK controls protein export.
Article
Multidisciplinary Sciences
Rei Nakamoto, Jeric Mun Chung Kwan, Jasmine Fei Li Chin, Hui Ting Ong, Josue Flores-Kim, Caroline Midonet, Michael S. VanNieuwenhze, Xue Li Guan, Lok-To Sham
Summary: Many pathogenic bacteria rely on a layer of capsular polysaccharide (CPS) for virulence, with the bacterial tyrosine kinase (BY-kinase) playing a crucial role in regulating capsule synthesis. The interaction between BY-kinase CpsCD and other proteins influences CPS polymer length and peptidoglycan synthesis in Streptococcus pneumoniae. Autophosphorylation of BY-kinases fine-tunes the activity of CpsC, providing spatiotemporal coordination between CPS and PG synthesis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Infectious Diseases
Fereshte Sheybani, Matthijs C. Brouwer, Diederik van de Beek
Summary: Bacterial meningitis in patients with multiple myeloma is rare but severe, with high rates of unfavorable outcomes. These patients often present with a lower cerebrospinal fluid leukocyte count compared to those without multiple myeloma. Physicians should have a low threshold for performing lumbar puncture and initiating antibiotic treatment in these patients.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Leigh M. Howard, Xiang Huang, Wencong Chen, Yuhan Liu, Kathryn M. Edwards, Marie R. Griffin, Yuwei Zhu, Jorge E. Vidal, Keith P. Klugman, Ana I. Gil, Nicole R. Soper, Isaac P. Thomsen, Katherine Gould, Jason Hinds, Claudio F. Lanata, Carlos G. Grijalva
Summary: We investigated the association between nasopharyngeal pneumococcal co-colonization and pneumococcal density in young Peruvian children. Pneumococcal co-colonization was common but not associated with increased pneumococcal density.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Biochemistry & Molecular Biology
Britta E. Rued, Jiaqi J. Zheng, Andrea Mura, Ho-Ching T. Tsui, Michael J. Boersma, Jeffrey L. Mazny, Federico Corona, Amilcar J. Perez, Daniela Fadda, Linda Doubravova, Karolina Buriankova, Pavel Branny, Orietta Massidda, Malcolm E. Winkler
MOLECULAR MICROBIOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Noelia Bernardo-Garcia, Kiran V. Mahasenan, Maria T. Batuecas, Mijoon Lee, Dusan Hesek, Denisa Petrackova, Linda Doubravova, Pavel Branny, Shahriar Mobashery, Juan A. Hermoso
ACS CHEMICAL BIOLOGY
(2018)
Article
Immunology
Pavla Stojkova, Petra Spidlova, Juraj Lenco, Helena Rehulkova, Lucie Kratka, Jiri Stulik
Article
Immunology
Petra Spidlova, Pavla Stojkova, Vera Dankova, Iva Senitkova, Marina Santic, Dominik Pinkas, Vlada Philimonenko, Jiri Stulik
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2018)
Review
Immunology
Pavla Stojkova, Petra Spidlova, Jiri Stulik
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Ales Ulrych, Denisa Petrackova, Jana Goldova, Karolina Buriankova, Linda Doubravova, Pavel Branny
Article
Chemistry, Physical
Michal Rosulek, Petra Darebna, Petr Pompach, Lukas Slavata, Petr Novak
Review
Microbiology
Petra Spidlova, Pavla Stojkova, Anders Sjostedt, Jiri Stulik
Article
Biochemistry & Molecular Biology
Radek Indra, Katarina Vavrova, Petr Pompach, Zbynek Heger, Petr Hodek
Article
Biochemistry & Molecular Biology
Ales Ulrych, Ivo Fabrik, Rudolf Kupcik, Marie Vajrychova, Linda Doubravova, Pavel Branny
Summary: The study highlights the critical role of StkP in coordinating cell division, cell wall synthesis, and adaptive responses in Streptococcus pneumoniae. Through phosphorylation of key proteins, StkP influences the regulation of the cell cycle and cell wall homeostasis.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Immunology
Pavla Pavlik, Petra Spidlova
Summary: This study identified the binding sites and binding motifs of HU protein in Francisella tularensis FSC200, and demonstrated the importance of HU protein in the pathogenesis and immune protection of FSC200. These findings deepen our understanding of the role of HU protein in tularaemia pathogenesis and suggest its significance in vaccine development.
Review
Immunology
Pavla Stojkova, Petra Spidlova
Summary: HU protein, a member of the nucleoid-associated protein family, plays a crucial role in regulating bacterial virulence, pathogenesis, and survival. Recent studies suggest that HU protein may not only exist inside bacteria but also be secreted outside bacteria and even enter host cells. However, its specific role in host cells is still not well understood and further research is needed.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Pavla Pavlik, Petr Jost, Pavel Rehulka, Vera Vozandychova, Marek Link, Petra Spidlova
Summary: EGCG can suppress the growth of Francisella tularensis, reduce its replication inside mouse bone marrow-derived macrophages, and block the main functions of HU protein, leading to overall attenuation of F. tularensis viability. It can delay death in mice infected by F. tularensis and can be used as a prophylactic agent against tularemia disease.
MICROBIAL PATHOGENESIS
(2023)
Article
Biochemistry & Molecular Biology
Ho-Ching Tiffany Tsui, Merrin Joseph, Jiaqi J. J. Zheng, Amilcar J. J. Perez, Irfan Manzoor, Britta E. E. Rued, John D. D. Richardson, Pavel Branny, Linda Doubravova, Orietta Massidda, Malcolm E. E. Winkler
Summary: The protein GpsB links peptidoglycan synthesis and cell shape determination in Streptococcus pneumoniae. Three classes of chromosomal duplications containing murZ or murA genes were found to suppress Delta gpsB or Delta StkP phenotypes. These findings demonstrate the ability of pneumococcus to modulate gene dosage and regulate protein phosphorylation.
MOLECULAR MICROBIOLOGY
(2023)
Article
Immunology
Vera Vozandychova, Pavel Rehulka, Kamil Hercik, Petra Spidlova, Pavla Pavlik, Jaroslav Hanus, Romana Hadravova, Jiri Stulik
Summary: Francisella tularensis interferes with the host's ubiquitination system by regulating the activity of deubiquitinating enzymes to escape degradation.
FRONTIERS IN IMMUNOLOGY
(2023)