Article
Multidisciplinary Sciences
Songwei Ni, Baiyuan Li, Kaihao Tang, Jianyun Yao, Thomas K. Wood, Pengxia Wang, Xiaoxue Wang
Summary: The study reveals that the type II TA system, Pseudoalteromonas rubra plasmid toxin-antitoxin PrpT/PrpA, directly controls plasmid replication by acting as a negative regulator. The antitoxin PrpA binds to iterons in the plasmid origin to inhibit the binding of the replication initiator, preventing overreplication of the plasmid. This TA system is conserved and widespread on many conjugative plasmids.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Genetics & Heredity
Aya Takashima, Hibiki Kawano, Tomomi Ueda, Chiho Suzuki-Minakuchi, Kazunori Okada, Hideaki Nojiri
Summary: The study identified a TA system (prcA/T) that can improve the stability of unstable plasmids in P. resinovorans and E. coli strains.
Article
Chemistry, Multidisciplinary
Zhigang Yu, Emily C. A. Goodall, Ian R. R. Henderson, Jianhua Guo
Summary: This study reveals that plasmid-free bacterial cells exhibit filamentation and asymmetrical cell division under exposure to sub-inhibitory concentrations of antibiotics, while plasmid-bearing cells do not show such morphological changes. Higher expression of sulA is observed in plasmid-free cells. The benefits of plasmid carriage include less DNA damage, stronger efflux pump activities, and higher antibiotic tolerance.
Article
Microbiology
Yusuke Ota, Isaac Prah, Samiratu Mahazu, Yoshiaki Gu, Yoko Nukui, Ryuji Koike, Ryoichi Saito
Summary: In this study, bla(GES)-encoding plasmids were characterized from a single-hospital sewage sample in Japan. The results showed that bla(GES) genes were mainly located on different types of plasmids, which were closely related to plasmids from Japan, the United States, and Europe. The bla(GES) genes were located on the class 1 integron cassette of the Tn3 transposon-related region. These findings provide insights into the diversity and spread of resistance and highlight the importance of continuous monitoring of environmental GES-type carbapenemase producers.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Jing Yang, Yujuan Tian, Huayi Liu, Yeyi Kan, Yi Zhou, Ying Wang, Yunzi Luo
Summary: The study improved episomal plasmids in Saccharomyces cerevisiae by enhancing stability and plasmid copy numbers, successfully constructing the tyrosol biosynthetic pathway, and achieving higher production yields.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Maelle Duperray, Jean-Marie Francois, Jean-Pascal Capp
Summary: This study provides a detailed characterization of the functioning of a bacterial toxin-antitoxin system in Saccharomyces cerevisiae and explores the application of these systems in controlling yeast growth.
FEMS YEAST RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Bo-Chou Chen, Yu-Zhen Chen, Huan-Yu Lin
Summary: The CRISPR-Cas system is widely used for genome editing due to its convenience, simplicity, and flexibility. This article describes a plasmid-curing strategy based on the CRISPR-Cpf1 system and proposes a simplified plasmid-curing system. The method efficiently removes the plasmid and allows for iterative processes in multiple rounds of genome editing.
Article
Microbiology
Bo-Xuan Zeng, Ming-Dong Yao, Wen-Hai Xiao, Yun-Zi Luo, Ying Wang, Ying-Jin Yuan
Summary: Editing the pE2 mu plasmid using CRISPR/Cas9 and constructing a multi-copy system can enhance plasmid stability and copy number in yeast, leading to more efficient gene expression and higher productivity. This study presents an improved expression system in yeast for biosynthesis pathway construction and valuable product production.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Alagesan Karthika, Balajee Ramachandran, Jeyarajpandian Chitra, Dhamodharan Prabhu, Sundaraj Rajamanikandan, Malaisamy Veerapandiyan, Jeyaraman Jeyakanthan
Summary: The majority of bacteria and archaea have a Toxin-Antitoxin system, where the Antitoxin inhibits Toxin activity but can be degraded, leading to Toxin activation. The Zeta Toxin-Epsilon Antitoxin system in ESKAPE pathogen stabilizes resistance plasmids. The mechanism of Zeta Toxin-Epsilon Antitoxin in cell wall biosynthesis remains unknown in Acinetobacter baumannii.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)
Article
Microbiology
Daniel Wilkinson, Lolwah Alsharaf, Stephen Thompson, Andreja Paulin, Rhodrick Takor, Abed Zaitoun, Karen Robinson, Jonathan Thomas, Gareth McVicker, Jody Winter
Summary: This study aimed to determine the association between biofilm-forming ability of H. pylori isolates and disease severity. The experiment revealed that there was no significant association between biofilm-forming ability and disease severity in H. pylori. However, the study identified a strain, 444A, with high biofilm-forming ability.
JOURNAL OF MEDICAL MICROBIOLOGY
(2023)
Review
Food Science & Technology
Lidia Boss, Barbara Kedzierska
Summary: Toxin-antitoxin (TA) systems are widely present in bacterial genomes and consist of stable toxins and unstable antitoxins. Cross-interactions between toxins and antitoxins of non-cognate modules may disrupt the balance and increase free toxin levels, which can be harmful to cells. TA systems can also regulate gene expression and cellular mRNA stability. Understanding the potential cross-interactions of TA systems is important for their practical applications in biotechnology and medicine.
Article
Microbiology
Chenchen Wang, Chuanying Niu, Khan Muhammad Hidayatullah, Lu Xue, Zhongliang Zhu, Liwen Niu
Summary: This study reported the structures of PrpA and PrpT, revealing the heterotetramer structure of PrpT:PrpA(2):PrpT and studying the oligomeric forms of PrpA. Based on these structures, a model of PrpA(2-54) homotetramer in complex with prpAT promoter and the improved mechanism underlying how PrpTA controls plasmid replication were proposed.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
David G. Nickens, Matthew L. Bochman
Summary: DNA helicases play a crucial role in nucleic acid transactions and genome integrity. Research has shown that the PIF1 (Pif1 and Rrm3) and RecQ (Hrq1 and Sgs1) family helicases exhibit genetic and biochemical interactions in vivo, affecting genome integrity pathways and telomerase activity.
Review
Microbiology
Muhammad Kamruzzaman, Alma Y. Wu, Jonathan R. Iredell
Summary: TA systems, commonly found in bacterial chromosomes and MGEs, play important roles in maintaining genetic material and regulating bacterial physiology, although there are still controversies surrounding their functions.
Article
Multidisciplinary Sciences
Yu Chen, Jens Nielsen
Summary: In this study, the in vivo catalytic rates of the yeast Saccharomyces cerevisiae under various conditions were estimated and compared with in vitro enzyme activity, revealing a weak correlation and considerable deviations between in vivo and in vitro enzyme activities. The correlation was improved after removing enzymes obtained through heterologous expression, but still not as strong as for bacteria. Parameterizing an enzyme-constrained metabolic model with the kapp dataset showed better performance in predicting proteomics data compared to using in vitro kcat, highlighting the importance of the generated dataset.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Anna Lukasik, Iwona Brzozowska, Urszula Zielenkiewicz, Piotr Zielenkiewicz
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Review
Pharmacology & Pharmacy
Kamil Zalewski, Malgorzata Benke, Bartlomiej Mirocha, Jakub Radziszewski, Magdalena Chechlinska, Magdalena Kowalewska
CURRENT PHARMACEUTICAL DESIGN
(2018)
Article
Oncology
Sebastian Zieba, Artur Kowalik, Kamil Zalewski, Natalia Rusetska, Krzysztof Goryca, Agata Piascik, Marcin Misiek, Elwira Bakula-Zalewska, Janusz Kopczynski, Kamil Kowalski, Jakub Radziszewski, Mariusz Bidzinski, Stanislaw Gozdz, Magdalena Kowalewska
GYNECOLOGIC ONCOLOGY
(2018)
Article
Engineering, Biomedical
Marek Konop, Joanna Czuwara, Ewa Klodzinska, Anna K. Laskowska, Urszula Zielenkiewicz, Iwona Brzozowska, Seyed M. Nabavi, Lidia Rudnicka
JOURNAL OF BIOMATERIALS APPLICATIONS
(2018)
Article
Ecology
Anna Szafranek-Nakonieczna, Agnieszka Wolinska, Urszula Zielenkiewicz, Agnieszka Kowalczyk, Zofia Stepniewska, Mieczyslaw Blaszczyk
Article
Agronomy
Agnieszka Wolinska, Dorota Gorniak, Urszula Zielenkiewicz, Agnieszka Kuzniar, Dariusz Izak, Artur Banach, Mieczyslaw Blaszczyk
Article
Multidisciplinary Sciences
Barbara Zyzynska-Granica, Bartosz Trzaskowski, Malgorzata Dutkiewicz, Oliwia Zegrocka-Stendel, Maja Machcinska, Katarzyna Bocian, Magdalena Kowalewska, Katarzyna Koziak
SCIENTIFIC REPORTS
(2020)
Review
Oncology
Sebastian Zieba, Magdalena Chechlinska, Artur Kowalik, Magdalena Kowalewska
GYNECOLOGIC ONCOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Sebastian Zieba, Anne-Floor W. Pouwer, Artur Kowalik, Kamil Zalewski, Natalia Rusetska, Elwira Bakula-Zalewska, Janusz Kopczynski, Johanna M. A. Pijnenborg, Joanne A. de Hullu, Magdalena Kowalewska
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Genetics & Heredity
Joanna Klim, Urszula Zielenkiewicz, Anna Kurlandzka, Szymon Kaczanowski, Marek Skoneczny
Article
Oncology
Agnieszka Fatalska, Natalia Rusetska, Elwira Bakula-Zalewska, Artur Kowalik, Sebastian Zieba, Agnieszka Wroblewska, Kamil Zalewski, Krzysztof Goryca, Dominik Domanski, Magdalena Kowalewska
Summary: Our study aimed to identify protein markers of VSCC tumors for patient stratification based on progression risk. Mass spectrometry-based proteomics revealed immune response as the most over-represented category for differentially expressed proteins (DEPs) in progVSCC vs. d-fVSCC tumors, with pathway profiling indicating bacterial infections linked to aggressive VSCC phenotypes. HMGA2 and PRTN3 were identified as proteins predicting VSCC progression and show promise as markers for patient stratification. Bacterial infections triggering an inflammatory response seem to contribute to cancer progression in VSCC, suggesting that targeted therapies should consider bacterial rather than viral infection status.
Article
Pathology
Natalia Rusetska, Kamil Kowalski, Kamil Zalewski, Sebastian Zieba, Mariusz Bidzinski, Krzysztof Goryca, Beata Kotowicz, Malgorzata Fuksiewicz, Janusz Kopczynski, Elwira Bakula-Zalewska, Artur Kowalik, Magdalena Kowalewska
Summary: The study found that the CXCR4/ACKR3/CXCL12 axis is activated during progression and lymphatic spread of VSCC. CXCR4 and ACKR3 proteins are nearly absent in precancerous lesions but are strong in VSCC samples. Additionally, serum CXCL12 levels are significantly increased in both patients with VSCC and premalignant vulvar lesions.
JOURNAL OF CLINICAL PATHOLOGY
(2022)
Editorial Material
Genetics & Heredity
Joanna Klim, Urszula Zielenkiewicz, Anna Kurlandzka, Szymon Kaczanowski
FRONTIERS IN GENETICS
(2021)
Article
Medicine, General & Internal
Mateusz Bujko, Kamil Zalewski, Martyna Szczyrek, Artur Kowalik, Joanna Boresowicz, Angelika Dlugosz, Krzysztof Goryca, Stanislaw Gozdz, Magdalena Kowalewska
Summary: This study investigated the diagnostic value of circulating hsa-miR-431-5p in vulvar precancers and VSCC. Higher levels of miR-431-5p were observed in VSCC patients compared to those with vulvar precancers, and its levels were predictive of survival rates in VSCC patients.
Article
Medicine, Research & Experimental
Natalia Rustetska, Magdalena Szczepaniak, Krzysztof Goryca, Elwira Bakula-Zalewska, Malgorzata Figat, Artur Kowalik, Stanislaw Gozdz, Magdalena Kowalewska
Summary: This study identified tumour-promoting bacteria in vulvar squamous cell carcinoma (VSCC), which are associated with neutrophil recruitment and cancer progression.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)