Article
Immunology
Chin-Chung Tseng, Wei-Hung Lin, An-Bang Wu, Ming-Cheng Wang, Ching-Hao Teng, Jiunn-Jong Wu
Summary: This study confirms the advantage and synergistic action of FimH and PapGII in E.coli kidney infection through comparing the prevalence and coexistence pattern of adhesin genes in upper and lower urinary tract infection strains, as well as evaluating the infection ability in a mouse model.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2022)
Article
Immunology
Ching-Hsun Wang, Ling Ma, Li-Yueh Huang, Kuo-Ming Yeh, Jung-Chung Lin, L. Kristopher Siu, Feng-Yee Chang
Summary: This study investigated the molecular epidemiology and resistance patterns of bla(OXA)-48 Klebsiella pneumoniae and Escherichia coli in Taiwan. The isolates showed high-level carbapenem resistance and broad resistance against many beta-lactam antibiotics, with the presence of multiple clones dissemination in bla(OXA-48) K. pneumoniae.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2021)
Article
Microbiology
Christopher J. Day, Alvin W. Lo, Lauren E. Hartley-Tassell, M. Pilar Argente, Jessica Poole, Nathan P. King, Joe Tiralongo, Michael P. Jennings, Mark A. Schembri
Summary: This study focused on dissecting the receptor specificity of different CU fimbriae in Escherichia coli using a glycan array analysis approach with whole-bacterial-cell surface plasmon resonance. The research revealed new information on fimbrial specificity and provided a rapid and scalable system to define novel adhesin-glycan interactions underlying bacterial colonization and disease. The findings contribute to a better understanding of bacterial adhesion mechanisms and offer a promising strategy to combat antibiotic resistance through blocking adhesion.
Article
Biochemistry & Molecular Biology
Sumeet Manandhar, Amy Scott-Thomas, Michael Harrington, Priyanka Sinha, Anna Pilbrow, Arthur Mark Richards, Vicky Cameron, Madhav Bhatia, Stephen T. Chambers
Summary: This study measured the concentrations of hydrogen sulfide (H2S) and substance P (SP) in the peripheral blood of patients with Escherichia coli or Klebsiella pneumoniae bacteraemia. The study found that the concentrations of H2S and SP varied with the site of infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Ola H. Moghnia, Nourah A. Al-Sweih
Summary: The spread of carbapenem-resistant E. coli and K. pneumoniae is a global concern. This study aimed to investigate the genetic characteristics and variations of multidrug resistant isolates. Whole genome sequencing was carried out on eight carbapenem-resistant isolates, and various resistance genes were detected. This research is important for understanding and combating antimicrobial resistance.
Article
Chemistry, Multidisciplinary
Yishuai Lin, Ying Zhang, Shixing Liu, Dandan Ye, Liqiong Chen, Na Huang, Weiliang Zeng, Wenli Liao, Yizhou Zhan, Tieli Zhou, Jianming Cao
Summary: Colistin, considered as a last resort treatment for Gram-negative bacteria infections, is becoming ineffective against colistin-resistant strains of Escherichia coli and Klebsiella Pneumoniae. The combination of quercetin with colistin efficiently eradicates colistin-resistant bacteria by downregulating resistance genes and enhancing bacterial cell membrane damage. This colistin/quercetin combination shows promise as an alternative pathway against colistin-resistant infections.
FRONTIERS IN CHEMISTRY
(2021)
Article
Microbiology
Samanta Freire, Teresa Grilo, Bruna Rodrigues, Rui Oliveira, Carla Esteves, Antonio Marques, Laurent Poirel, Marta Aires-de-Sousa
Summary: This study aimed to analyze the occurrence and characteristics of ESBL- and carbapenemase-producing E. coli among bivalves. Out of 522 collected bivalve samples, E. coli growth was observed in 39% of the samples. Nine samples (4.4%) were contaminated with ESBL producers and one sample (0.5%) was contaminated with a carbapenemase-producing K. pneumoniae. The presence of ESBL- and carbapenemase-producing Enterobacteriaceae in bivalves indicates that the marine environment could be a reservoir of critical bacterial pathogens, posing a potential risk to human health.
Article
Infectious Diseases
Alix Benedicte Kagambega, Rene Dembele, Lea Bientz, Fatima M'Zali, Laure Mayonnove, Alassane Halawen Mohamed, Hiliassa Coulibaly, Nicolas Barro, Veronique Dubois
Summary: This study screened for carbapenemase-producing bacteria in hospital wastewater and found the presence of clinically relevant isolates with multiple resistance determinants. These findings highlight the need for intensified antimicrobial resistance surveillance.
Article
Infectious Diseases
Fuxing Li, Junqi Zhu, Yaping Hang, Yanhui Chen, Shumin Gu, Suqin Peng, Youling Fang, Longhua Hu, Jianqiu Xiong
Summary: This study aimed to investigate the variations in clinical features and prognosis of HABP caused by E. coli and K. pneumoniae. Among the HABP patients, a higher proportion of males, ICU admission, carbapenem-resistant strains, and other risk factors were observed in the K. pneumoniae group compared to the E. coli group. The 30-day mortality rate was also higher in the K. pneumoniae group. The findings suggest that the clinical features and prognosis of HABP vary depending on the causative bacteria.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Immunology
Joshua T. Thaden, Richard Ahn, Felicia Ruffin, David W. Gjertson, Alexander Hoffmann, Vance G. Fowler, Michael R. Yeaman
Summary: This study identifies reduced host transcriptional signatures involving mitochondrial energy transduction in methicillin-resistant Staphylococcus aureus compared to methicillin-susceptible Staphylococcus aureus. Patients with gram-negative infection had increased activation of the classic complement system.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Microbiology
Jose Manuel Ortiz de la Rosa, Angel Rodriguez-Villodres, Maria Adelina Gimeno Gascon, Guillermo Martin-Gutierrez, Jose Miguel Cisneros, Jose Antonio Lepe
Summary: As resistance to TZP in K. pneumoniae and E. coli increases, rapid determination of susceptibility to piperacillin-tazobactam (TZP) is crucial for the treatment of infected patients. In this study, the RapidTZP test was developed to detect TZP resistance in a maximum of 3h, with a sensitivity and specificity of 98.24% and 100%, respectively.
MICROBIOLOGY SPECTRUM
(2023)
Article
Immunology
Achiraya Siriphap, Thawatchai Kitti, Akachai Khuekankaew, Chalermchai Boonlao, Chonthida Thephinlap, Chutamas Thepmalee, Nittiya Suwannasom, Krissana Khoothiam
Summary: This study investigated the prevalence of ESBL-positive E. coli and K. pneumoniae isolates from hospitalized patients in Chiangrai Prachanukroh hospital, Thailand. The study found a high prevalence and emerging antibiotic resistance of ESBL-positive strains, indicating the need for carbapenems and aminoglycosides as the first-line drugs.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Yi-Yun Liu, Xiao-Qing Zhu, Sue C. Nang, Haoliang Xun, Luchao Lv, Jun Yang, Jian-Hua Liu
Summary: The prevalence of mcr-1 is higher in E. coli than in K. pneumoniae due to the greater transferability and persistence of mcr-1-bearing plasmids in the former species.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Yanyan Zhang, Xuemei Yang, Congcong Liu, Ling Huang, Lingbin Shu, Qiaoling Sun, Hongwei Zhou, Yonglu Huang, Chang Cai, Xiaoyan Wu, Sheng Chen, Rong Zhang
Summary: This study investigated the prevalence and characteristics of OXA-232 type carbapenemase in Zhejiang Province, China from 2018 to 2021. The results showed that OXA-232 is the most prevalent OXA-48-like derivative, and ST15 K. pneumoniae isolates are the major carriers. The transmission of ColKP3-type plasmid to E. coli highlighted the importance of understanding the transmission mechanism to prevent the spread of OXA-232 to other species.
INFECTIOUS DISEASES OF POVERTY
(2023)
Article
Infectious Diseases
Wenjuan Yan, Qi Zhang, Yingjie Zhu, Nan Jing, Youhua Yuan, Yi Zhang, Siying Ren, Dongmei Hu, Wenmin Zhao, Xiaojuan Zhang, Caiqin Shi, Meiyun Wang, Yi Li
Summary: The study evaluated the prevalence and characteristics of polymyxin-resistant Klebsiella pneumoniae and Escherichia coli in Henan province, China. It was found that the polymyxin resistance rate was slightly higher in K. pneumoniae compared to E. coli, and mcr-1 was more commonly detected in E. coli than in K. pneumoniae. Additionally, the insertion of ISkpn14 into mgrB may play a key role in polymyxin resistance in K. pneumoniae in Henan.
INFECTION AND DRUG RESISTANCE
(2021)
Review
Public, Environmental & Occupational Health
Lars Andrup, Karen A. Krogfelt, Kristian Schultz Hansen, Anne Mette Madsen
Summary: This study systematically reviewed the literature on the transmission routes of human rhinoviruses. The results showed that indirect transmission through hands and fomites is the dominant route of transmission in indoor settings, while airborne transmission is also a major route of transmission.
AMERICAN JOURNAL OF INFECTION CONTROL
(2023)
Article
Microbiology
Simon Meski, Carsten Struve, Hengameh C. Mirsepasi-Lauridsen, Andreas M. Petersen, Lotte Jelsbak, Ole Skovgaard, Karen A. Krogfelt
Summary: This study reports the complete genome and plasmid sequences of three Escherichia coli isolates, C 237-04 (p7), C 236-04A (p10A), and C 691-04A (p19A), obtained from fecal samples from ulcerative colitis patients in Copenhagen, Denmark, revealing the potential role of extraintestinal pathogenic Escherichia coli (ExPEC) in the etiology of ulcerative colitis.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Immunology
Siv Fonnes, Tilde Rasmussen, Barbara Juliane Holzknecht, Christoffer Skov Olesen, Joachim Hjalde Halmsted Olsen, Line Schmidt, Rasmus Alder, Sara Gamborg Sorensen, Sengul Gulen, Louise Laurberg Klarskov, Karen Angeliki Krogfelt, Henrik Westh, Jacob Rosenberg
Summary: This study investigated the presence of diarrhoea-causing bacteria in patients undergoing surgery for suspected appendicitis. The findings suggest that infection with Yersinia spp. and other diarrhoea-causing microorganisms is rare in these patients.
Article
Public, Environmental & Occupational Health
Mette Frimodt Hansen, Pelle Korsbaek Sorensen, Anja Elaine Sorensen, Karen Angeliki Krogfelt
Summary: The perceived seriousness of tick bites and Lyme borreliosis significantly predicts the adoption of protective measures against ticks. The perceived likelihood of getting a tick bite or Lyme borreliosis also predicts the adoption of protection, although the impact is small.
Article
Infectious Diseases
Noah Obeng-Nkrumah, Dennis Schroder Hansen, Georgina Awuah-Mensah, Nana Kweiba Blankson, Niels Frimodt-Moller, Mercy Jemima Newman, Japheth Awuletey Opintan, Karen Angeliki Krogfelt
Summary: A cross-sectional survey was conducted in eight Ghanaian communities to investigate the extent of intestinal colonization with 3rd-generation cephalosporin-resistant Enterobacterales. The study found that 50.4% of the participants carried 3rd-generation cepha-losporin-resistant E. coli and K. pneumoniae. Most of these were ESBL-producing E. coli, carrying CTX-M genes, mainly CTX-M-15. The presence of a household toilet facility was significantly associated with a reduced risk of intestinal colonization.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2023)
Article
Microbiology
Rosa Maja Mohring Gynthersen, Mette Frimodt Hansen, Lukas Frans Ocias, Andreas Kjaer, Randi Fons Petersen, Sisse Rye Ostrowski, Lene Harritshoj, Soren Jacobsen, Ulrik Overgaard, Karen Angeliki Krogfelt, Anne-Mette Lebech, Helene Mens
Summary: The presence of N. mikurensis DNA was detected in immunocompromised patients in Denmark, highlighting the potential underdiagnosis of this bacterium in Danish patients.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2023)
Article
Immunology
Caroline Eves, Charlotte Kjelso, Guido Benedetti, Charlotte Svaerke Jorgensen, Karen Angeliki Krogfelt
Summary: This study describes the incidence trends of leptospirosis in Denmark from 2012 to 2021. Leptospirosis is rare in Denmark and primarily transmitted to humans from mice and rats. The highest incidence of the disease occurred in 2017, with men aged 40-49 being the most commonly diagnosed demographic group. The overall incidence rate was 0.23 per 100,000 inhabitants, with the months of August and September having the highest incidence.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Sandra Meinich Juhl, Karen Angeliki Krogfelt, Witold Kot, Dennis Sandris Nielsen, Lukasz Krych
Summary: This study used 16S rRNA gene amplicon sequencing to analyze the microbial profile of 94 used nasogastric tubes collected from a neonatal intensive care unit. The results showed that the most common Gram-negative bacteria in the tubes were Enterobacteriaceae, Klebsiella, and Serratia, while the most common Gram-positive bacteria were staphylococci and streptococci. The microbial profile of the tubes was mainly determined by individual-specific bacteria and was not influenced by the duration of use, but strongly influenced by the environment.
Review
Infectious Diseases
Ebenezer Krampah Aidoo, Frank Twum Aboagye, Felix Abekah Botchway, George Osei-Adjei, Michael Appiah, Ruth Duku-Takyi, Samuel Asamoah Sakyi, Linda Amoah, Kingsley Badu, Richard Harry Asmah, Bernard Walter Lawson, Karen Angeliki Krogfelt
Summary: Reactive case detection (RACD) is a recommended strategy for detecting and eliminating asymptomatic malaria. Through systematic review, this study found that individuals living in households with index cases were at a higher risk of malaria infection compared to neighbors of index cases. The identification and treatment of infectious reservoirs, including neighboring households, are critical for successful malaria elimination.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2023)
Article
Infectious Diseases
Marc Westerholt, Karen Angeliki Krogfelt, Ram Benny Dessau, Lukas Frans Ocias
Summary: Lyme borreliosis (LB) is a common tick-transmitted infection caused by bacteria. This study proposes a method for determining actual changes in IgG and examines antibody reactivity and decay. The results show that both IgG and IgM antibodies persisted for years, with longer seropositivity associated with high initial antibody levels and IgG-type antibodies.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Hematology
Camous Moslemi, Susanne G. Saekmose, Rune Larsen, Jakob T. Bay, Thorsten Brodersen, Maria Didriksen, Henrik Hjalgrim, Karina Banasik, Kaspar R. Nielsen, Mie T. Bruun, Joseph Dowsett, Khoa M. Dinh, Susan Mikkelsen, Christina Mikkelsen, Thomas F. Hansen, Henrik Ullum, Christian Erikstrup, Soren Brunak, Karen Angeliki Krogfelt, Jill R. Storry, Sisse R. Ostrowski, Martin L. Olsson, Ole B. Pedersen
Summary: This study demonstrates the viability and accuracy of predicting blood types using existing genotype data. It successfully narrowed down the pool of potential donors with rare blood types and provided a more comprehensive overview of blood types.
Review
Infectious Diseases
Hanne Quarsten, Anna Henningsson, Karen Krogfelt, Christina Strube, Christine Wenneras, Sally Mavin
Summary: The impact of tick-borne diseases caused by various pathogens on public health, particularly Anaplasma phagocytophilum, Neoehrlichia mikurensis, Borrelia miyamotoi, Rickettsia helvetica, and Babesia species, remains largely unknown. This study reports and discusses data on the prevalence of these pathogens in Ixodes ricinus ticks from seven countries within the North Sea Region in Europe, as well as the diagnostic tests available and the main clinical features of their corresponding diseases. Increased awareness is crucial to identify cases of these under-recognized tick-borne diseases, providing valuable insights into their clinical significance.
TICKS AND TICK-BORNE DISEASES
(2023)
Article
Multidisciplinary Sciences
Rie Jonsson, Alexander Bjorling, Soren Roi Midtgaard, Grethe Vestergaard Jensen, Nicholas Skar-Gislinge, Lise Arleth, Steve Matthews, Karen Angeliki Krogfelt, Havard Jenssen
Summary: This study used small-angle X-ray scattering to reveal the structural morphology of the aggregative adherence fimbriae. The results showed that the fimbriae were arranged in a compact manner with Agg5A + Agg3B subunit proteins assembled pairwise on a flexible string. The study also suggests that the minor subunit plays a significant role in the assembled fimbriae by affecting the overall structure.
SCIENTIFIC REPORTS
(2023)
Article
Immunology
Anders Frische, Vithiagaran Gunalan, Karen Angeliki Krogfelt, Anders Fomsgaard, Ria Lassauniere
Summary: This study identifies unique antibody targets associated with neutralization breadth through a combination of neutralizing antibody functional responses and B-cell epitope mapping. The results indicate that animals with intermediate and high cross-neutralization target fewer antigenic sites on the spike protein and focus on a specific epitope situated outside the receptor binding domain. The approach taken in this study can be applied to other viral vaccines and provides important insights for rational vaccine design against SARS-CoV-2.
Article
Microbiology
Pi Westi Bondegaard, Anders Meyer Torp, Priscila Guerra, Katja Ann Kristensen, Juliane Fjelrad Christfort, Karen Angeliki Krogfelt, Line Hagner Nielsen, Kinga Zor, Anja Boisen, Martin Steen Mortensen, Martin Iain Bahl, Tine Rask Licht
Summary: The use of microcontainers did not provide a competitive advantage for the probiotic E. coli Nissle in colonizing the gut of mice. Alternative approaches are needed to enhance the competitive capacity of EcN in the gut.
FEMS MICROBIOLOGY LETTERS
(2023)