Article
Mathematical & Computational Biology
Amit Kugler, Karin Stensjo
Summary: Understanding energy and redox homeostasis, as well as carbon partitioning, is crucial for systems metabolic engineering of cell factories. Stoichiometric metabolic modeling and network-wide analysis revealed that the rate of NAD(P)H regeneration controls the ATP/NADPH ratio, and energy and redox balance is interconnected with carbon and nitrogen metabolism. An auxiliary pathway was also identified to support cellular redox homeostasis and ATP cycling.
NPJ SYSTEMS BIOLOGY AND APPLICATIONS
(2023)
Article
Plant Sciences
Sandeesha Kodru, Laszlo Sass, Priyanka Patil, Milan Szabo, Imre Vass
Summary: In this study, a TL component similar to the plant AG band, originating from NDH-1-mediated CEF, was identified in cyanobacteria. The +40 degrees C band was most efficiently induced by FR illumination at -10 degrees C, and it was observed that low temperatures block CEF at two different sites in Synechocystis PCC 6803.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Engineering, Environmental
Javier Garcia-Calleja, Thibaut Cossart, Zoyne Pedrero, Joao P. Santos, Laurent Ouerdane, Emmanuel Tessier, Vera Slaveykova, David Amouroux
Summary: Understanding the complexation of mercury (Hg) with low molecular weight (LMW) bioligands can help elucidate its speciation, and the use of isotopically labeled Hg species in cyanobacteria has provided insights into the role of intracellular biogenic ligands in the speciation of iHg and MeHg.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Daniel Solymosi, Dmitry Shevela, Yagut Allahverdiyeva
Summary: The study demonstrates that the gaseous radical nitric oxide (NO) can block photosynthetic electron transfer in cyanobacterial cells by repressing PSII, PSI, and likely the NDH-1 complex. These findings reveal the profound effects of NO on cyanobacterial cells and emphasize the importance of controlled NO homeostasis in cyanobacteria.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2022)
Article
Biochemical Research Methods
Maurice Mager, Hugo Pineda Hernandez, Fabian Brandenburg, Luis Lopez-Maury, Alistair J. McCormick, Dennis J. Nuernberg, Tim Orthwein, David A. Russo, Angelo Joshua Victoria, Xiaoran Wang, Julie A. Z. Zedler, Filipe Branco dos Santos, Nicolas M. Schmelling
Summary: In recent years, many new synthetic biology tools have been developed for use in cyanobacteria. However, the reported characterizations of these tools often cannot be reproduced, which greatly limits comparability of results and applicability. This study assessed the reproducibility of a standard microbiological experiment in Synechocystis sp. PCC 6803 and found significant differences in spectrophotometer measurements and growth rates between laboratories. These findings highlight the need for additional reporting requirements of growth conditions for phototrophic organisms beyond light intensity and CO2 supply.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Plant Sciences
Yumeng Zhang, Yaqi Wang, Wei Wei, Min Wang, Shuzhao Jia, Mingkun Yang, Feng Ge
Summary: Protein homeostasis in cyanobacteria is regulated by a proteolytic complex called ClpXP, which consists of ATPase ClpX and peptidase ClpP. This study aimed to investigate the functions and regulatory networks of ClpX in Synechocystis by identifying 172 differentially expressed proteins upon clpX interruption. The results provide a comprehensive ClpX-regulated protein network and new candidates for future functional studies on ClpX in cyanobacteria.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Christopher J. Gisriel, Jimin Wang, Jinchan Liu, David A. Flesher, Krystle M. Reiss, Hao-Li Huang, Ke R. Yang, William H. Armstrong, M. R. Gunner, Victor S. Batista, Richard J. Debus, Gary W. Brudvig
Summary: The cryo-electron microscopy structure of PSII from Synechocystis sp. PCC 6803 has been determined at high resolution, revealing differences from thermophilic cyanobacterial PSII structures that significantly impact the understanding of PSII structure and the mechanism of water oxidation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Parveen Akhtar, Avratanu Biswas, Fanny Balog-Vig, Ildiko Domonkos, Laszlo Kovacs, Petar H. Lambrev
Summary: Cyanobacterial mutants with monomeric PSI show changes in the composition and abundance of phycobilisomes and in the excitation energy transfer to PSII and PSI. The oligomeric state of PSI impacts the excitation energy flow in Synechocystis.
Article
Biology
Kaisei Maeda, Yukiko Okuda, Gen Enomoto, Satoru Watanabe, Masahiko Ikeuchi
Summary: Extracellular polysaccharides of bacteria play crucial roles in biofilm formation, stress tolerance, and infectivity, with cyanobacteria uniquely producing sulfated polysaccharides to support phototrophic biofilms. Little is known about the biosynthesis and function of these polysaccharides in cyanobacteria, but a study on Synechocystis sp. strain PCC 6803 has identified the genes responsible for the synthesis of a sulfated polysaccharide called synechan, potentially leading to insights into the functions and biotechnological applications of similar polysaccharides found in cyanobacteria genomes.
Article
Biotechnology & Applied Microbiology
Kateryna Kukil, Elias Englund, Nick Crang, Elton P. Hudson, Pia Lindberg
Summary: In this study, phenylalanine resistant mutant strains of the unicellular cyanobacterium Synechocystis sp. PCC 6803 were generated by laboratory evolution. The mutant strains were able to secrete phenylalanine and showed potential for production of trans-cinnamic acid and para-coumaric acid. The PRM8 strain demonstrated the highest specific production of these compounds.
METABOLIC ENGINEERING
(2023)
Article
Microbiology
Dorota Muth-Pawlak, Sanna Kreula, Peter J. Gollan, Tuomas Huokko, Yagut Allahverdiyeva, Eva-Mari Aro
Summary: This study analyzed the proteomes of photosynthetic cyanobacteria under different conditions and found differential expression of proteins related to photosynthesis and carbon metabolism. The study also discovered unique expression patterns under specific conditions and concluded that combining photosynthetic activity with high intracellular inorganic carbon conditions promotes excellent growth in the bacteria.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Henna Mustila, Dorota Muth-Pawlak, Eva-Mari Aro, Yagut Allahverdiyeva
Summary: In this study, the global proteome of Synechocystis sp. PCC 6803 under fluctuating light conditions was compared with constant light conditions. It was found that cell metabolism was clearly reprogrammed, and there were correlations between mRNA and protein levels to some extent. The data suggest that higher nitrogen assimilation is maintained for long-term acclimation to fluctuating light upon CO2 step-down in Synechocystis sp. PCC 6803.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Biochemistry & Molecular Biology
Shiqi Lin, Shiliang Li, Tong Ouyang, Gu Chen
Summary: Excess ammonium causes toxicity and stress in cyanobacteria, and how cyanobacteria respond to ammonium stress is not well understood. In this study, a gene called Slr1821 was found to be a crucial regulator for ammonium stress response in Synechocystis sp. PCC6803. The expression of many genes involved in nitrogen uptake, assimilation, and carbon regulation depended on the presence of Slr1821, highlighting its importance in maintaining carbon/nitrogen balance under high nitrogen conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Takashi Inada, Shuntaro Machida, Koichiro Awai, Iwane Suzuki
Summary: Microalgal lipids and fatty acids are important components for biofuel production due to their high productivity potential. Genetic modification can lead to the synthesis of (omega-1)-hydroxy fatty acids in cyanobacteria, but high light conditions may inhibit photosynthesis and growth of the strain.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Prashant R. R. Singh, Jainendra Pathak, Rajneesh, Haseen Ahmed, Donat -P. P. Haeder, Rajeshwar P. Sinha
Summary: Cyanobacteria use circadian rhythms to cope with daily light fluctuations and the dark phase plays a significant role in protecting them from the negative impact of continuous light exposure. This study provides insights into the physiological responses of cyanobacteria to changing light environments.
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
(2023)
Article
Plant Sciences
Rocio Robles-Rengel, Francisco J. Florencio, M. Isabel Muro-Pastor
Article
Biochemical Research Methods
Rebeca Vidal, Monica Venegas-Caleron
JOURNAL OF MICROBIOLOGICAL METHODS
(2019)
Article
Plant Sciences
Sandra Diaz-Troya, Miguel Roldan, Manuel J. Mallen-Ponce, Pablo Ortega-Martinez, Francisco J. Florencio
JOURNAL OF EXPERIMENTAL BOTANY
(2020)
Article
Plant Sciences
M. Isabel Muro-Pastor, Aureo Cutillas-Farray, Laura Perez-Rodriguez, Julia Perez-Saavedra, Ana Vega-de Armas, Ana Paredes, Rocio Robles-Rengel, Francisco J. Florencio
Article
Multidisciplinary Sciences
Raquel Garcia-Canas, Joaquin Giner-Lamia, Francisco J. Florencio, Luis Lopez-Maury
Summary: The study reveals the involvement of proteins PetR and PetP in regulating the soluble electron carrier in cells. Transcriptomic analysis shows that the PetRP system specifically regulates four genes (petE, petJ, slr0601, and slr0602), with their presence potentially representing an early adaptation to decreased iron bioavailability following the GOE.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
Paul Bolay, Rokhsareh Rozbeh, M. Isabel Muro-Pastor, Stefan Timm, Martin Hagemann, Francisco J. Florencio, Karl Forchhammer, Stephan Klaehn
Summary: Cyanobacteria have unique regulatory mechanisms to control metabolic processes, exemplified by small proteins like PirA regulating nitrogen flux. The interaction between PirA and P-II impacts arginine synthesis, suggesting potential applications in metabolic engineering for biotechnological purposes.
Article
Plant Sciences
Manuel J. Mallen-Ponce, Maria Jose Huertas, Ana Maria Sanchez-Riego, Francisco J. Florencio
Summary: The study reveals that the regulatory role of thioredoxins is conserved in oxygenic photosynthetic organisms, and depletion of TrxA greatly interferes with the cellular redox state, affecting essential biological processes.
Review
Biochemistry & Molecular Biology
Manuel J. Mallen-Ponce, Maria Jose Huertas, Francisco J. Florencio
Summary: This study focuses on the evolutionary diversity of Trx systems in cyanobacteria and their phylogenetic relationships, as well as the physiological relevance of redox regulation in cyanobacteria. The results reveal variations in Trx systems among different cyanobacteria and gradual loss of certain proteins with cyanobacterial diversification.
Article
Biochemistry & Molecular Biology
Paul Bolay, Luisa Hemm, Francisco J. Florencio, Wolfgang R. Hess, M. Isabel Muro-Pastor, Stephan Klaehn
Summary: This study reveals the mechanism by which cyanobacteria regulate metabolic pathways through the interaction of small protein inhibitors and sRNAs, further highlighting the importance of cyanobacteria in regulating metabolic flexibility in response to nitrogen fluctuations.
Article
Biochemistry & Molecular Biology
Carmen Castell, Encarnacion Diaz-Santos, Luis G. Heredia-Martinez, Luis Lopez-Maury, Jose M. Ortega, Jose A. Navarro, Mercedes Roncel, Manuel Hervas
Summary: In the diatom Phaeodactylum tricornutum, iron limitation leads to a decrease in the content of photosystem II and subtle changes in the energetics of the recombination reaction. Iron deficiency also reduces the binding of extrinsic subunits PsbV and PsbO to photosystem II. These findings suggest that iron plays a crucial role in the function and protein binding of photosystem II.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Pablo Ortega-Martinez, Miguel Roldan, Sandra Diaz-Troya, Francisco J. Florencio
Summary: Glycogen and starch are important polysaccharides for carbon and energy storage. The interconversion of glucose-1-phosphate and glucose-6-phosphate by phosphoglucomutases connects the metabolism of these polysaccharides with central carbon metabolism. The enzyme PMM/PGM plays a crucial role in supporting glycogen metabolism and its overexpression can overcome stress sensitivity in a PGM-null mutant.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Raquel Garcia-Canas, Francisco J. Florencio, Luis Lopez-Maury
Summary: Fructose-1,6-bisphosphatase (FBPase) and sedoheptulose-1,7-bisphosphatase (SBPase) are crucial activities in the Calvin-Benson-Bassham cycle. This study investigates the importance of these activities for photoautotrophic growth in Synechocystis sp PCC 6803. The results demonstrate that the co-expression of plant FBPase and SBPase genes can restore photoautotrophic growth in mutants lacking the SBP/FBPase protein. The redox state of the proteins may play a role in this phenotype, and the cyanobacterial redox system can reduce the plant proteins in a light-dependent manner.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemical Research Methods
Maurice Mager, Hugo Pineda Hernandez, Fabian Brandenburg, Luis Lopez-Maury, Alistair J. McCormick, Dennis J. Nuernberg, Tim Orthwein, David A. Russo, Angelo Joshua Victoria, Xiaoran Wang, Julie A. Z. Zedler, Filipe Branco dos Santos, Nicolas M. Schmelling
Summary: In recent years, many new synthetic biology tools have been developed for use in cyanobacteria. However, the reported characterizations of these tools often cannot be reproduced, which greatly limits comparability of results and applicability. This study assessed the reproducibility of a standard microbiological experiment in Synechocystis sp. PCC 6803 and found significant differences in spectrophotometer measurements and growth rates between laboratories. These findings highlight the need for additional reporting requirements of growth conditions for phototrophic organisms beyond light intensity and CO2 supply.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Luis Lopez-Maury, Luis G. Heredia-Martinez, Francisco J. Florencio
