Article
Biochemistry & Molecular Biology
Beatriz Javega, Guadalupe Herrera, Jose-Enrique O'Connor
Summary: This study validated a new experimental model to assess the specificity of fluorescent probes and different ROS species in a bacterial model. The results showed that the fluorescent probe MitoPY1 has higher sensitivity and specificity for detecting H2O2, while H2DCF-DA is more sensitive to organic peroxides. Additionally, there is a correlation between the lipophilicity of peroxides and their toxicity to bacteria. These findings help reduce experimental errors and provide recommendations for cytometric studies of oxidative stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Environmental Sciences
Ping Chen, Jiwei Jiang, Shixuan Zhang, Xinyu Wang, Xiaoyan Guo, Fengxiang Li
Summary: Microbial fuel cells (MFCs) can simultaneously generate electricity and treat antibiotic wastewater. However, the antibiotic resistance genes (ARGs) induced by antibiotics in MFCs increase risks to ecosystems and human health. This study evaluated the activities of enzymes and regulation genes related to ARGs in MFCs spiked with sulfamethoxazole (SMX) and found that the induction mechanism of ARGs varies with different SMX concentrations. Additionally, the exposure concentrations of SMX had no significant effect on the electricity production of MFCs.
Review
Chemistry, Multidisciplinary
Namrata Singh, G. R. Sherin, Govindasamy Mugesh
Summary: Nanozymes, nanomaterials with enzyme-mimicking activity, have attracted significant interest in biomedical applications, particularly in oxidative stress-mediated disorders. The challenge lies in designing nanozymes that can selectively differentiate between physiological oxidative stress and pathological oxidative stress and mediate appropriate redox reactions in cells, tissues, and organs. This review summarizes the advancements in redox-active nanozymes and their therapeutic significance in various disease models, such as cancer, neurodegeneration, and cardiovascular diseases. It also discusses the future prospects and challenges associated with the biomedical applications of nanozymes.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Engineering, Environmental
Falin He, Qiang Liu, Mingyang Jing, Jingqiang Wan, Chengqian Huo, Wansong Zong, Jingchun Tang, Rutao Liu
Summary: Phenanthrene (PHE) is a significant organic compound found widely in soil, which can induce oxidative stress and cytotoxicity in earthworm coelomocytes at high concentrations. The interaction between PHE and antioxidant enzymes SOD/CAT plays a role in these toxic effects.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Environmental Sciences
Zhihan Cui, Falin He, Xiangxiang Li, Yuze Li, Chengqian Huo, Hao Wang, Yuntao Qi, Guang Tian, Wansong Zong, Rutao Liu
Summary: This study reveals the response mechanisms of SOD/CAT under the regulation of TCC-induced oxidative stress and sheds new light on the toxic pathways of exogenous pollutants on antioxidant-related proteins function.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Horticulture
Mohammad Pourebrahimi, Saeid Eshghi, Asghar Ramezanian, Shiva Faghih
Summary: High salinity levels can lead to various adverse effects on plants, including osmotic stress and nutritional disorders. In this study, the combined use of hydrogen sulfide and selenium compounds was found to alleviate the negative effects of salt stress on strawberry yield and enhance the antioxidant and nutritional quality of the fruits.
SCIENTIA HORTICULTURAE
(2023)
Review
Biotechnology & Applied Microbiology
Giannina Espina, Joaquin Atalah, Jenny M. Blamey
Summary: The demand for enzymes in industrial processes is increasing globally, with extremophilic enzymes showing higher stability and activity, leading to diverse potential applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Dina Mulyanti, Sundani Nurono Soewandhi, Catur Riani
Summary: By inserting a synthetic rpoS coding region, the expression plasmid pCAD(2)_sod was modified to provide RpoS earlier and accumulate more target protein, resulting in a new plasmid pCAD(2)(+)_sod. The rMnSODSeq protein produced from pCAD(2)(+)_sod showed a 1.5-fold increase at 37 degrees C and higher intensity at 43 degrees C compared to pCAD(2)_sod, with an earlier shift in phase. The mRNA level from pCAD(2)(+)_sod was the lowest and had high relative quantification (RQ) towards the rho reference gene.
MOLECULAR BIOLOGY REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Pavlina Hemerkova, Martin Valis
Summary: ALS is a neurodegenerative disease that affects motor neurons and currently has no cure. Free oxygen radicals are known to play a role in the pathogenesis of ALS, while antioxidant enzymes like SOD1 are crucial for antioxidant protection.
Review
Biochemistry & Molecular Biology
Yu Ah Hong, Cheol Whee Park
Summary: Reactive oxygen and nitrogen species play crucial roles in kidney injuries, making antioxidant agents, especially catalytic antioxidants that mimic antioxidant enzymes, effective in combating oxidative stress-related kidney diseases. Research has shown the effectiveness of several catalytic antioxidants in treating disease models associated with oxidative stress.
Article
Environmental Sciences
Paolo Pastorino, Elisabetta Pizzul, Damia Barcelo, Maria Cesarina Abete, Gabriele Magara, Paola Brizio, Rosa Avolio, Marco Bertoli, Alessandro Dondo, Marino Prearo, Antonia Concetta Elia
Summary: This study investigated the oxidative stress ecology in Danube barbel from the Barbucina creek, analyzing the levels of antioxidant biomarkers and trace elements in the fish. Results indicated a significant association between certain trace elements and enzyme activity, suggesting their role as stressors in the aquatic environment. The study provides insights into the biochemical defensive response of fish to environmental disturbances, advancing our understanding of the effects of environmental factors on oxidative stress in aquatic ecosystems.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Review
Biochemistry & Molecular Biology
Diana Ofelia Lopez-Cantu, Reyna Berenice Gonzalez-Gonzalez, Elda M. Melchor-Martinez, Saul Antonio Hernandez Martinez, Rafael G. Araujo, Lizeth Parra-Arroyo, Juan Eduardo Sosa-Hernandez, Roberto Parra-Saldivar, Hafiz M. N. Iqbal
Summary: Nanozymes, artificial enzymes based on engineered nanomaterials, have been developed to overcome inherent drawbacks of natural enzymes, showing great potential for various applications. Carbon dots have garnered significant attention for their high catalytic activity and biocompatibility, serving as possible alternatives in biomedical and environmental fields. Studies have shown that carbon dots can mimic different oxidoreductases, exhibiting multiple enzyme-like activities.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Plant Sciences
Fatemeh Najafi, Gholamreza Kavoosi, Roghayeh Siahbalaei, Amina Kariminia
Summary: The study demonstrated that Agastache foeniculum essential oil and oil fraction have inhibitory effects on oxidative stress induced by hyperglycemia and inflammation, mainly by regulating the activity and expression levels of relevant enzymes to reduce the production of oxidative stress. At the molecular level, the components of AFEO and AFoil were found to interact with corresponding proteins through molecular docking, showing antioxidant and anti-diabetic effects.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
M. Dolores Garrido, Jamal El Haskouri, Maria D. Marcos, Francisco Perez-Pla, Jose Vicente Ros-Lis, Pedro Amoros
Summary: This study reports the preparation of a novel nanocomposite with enzyme-like activity for decomposing reactive oxygen species (ROS). The nanocomposite, consisting of MnOx needles covered with SiO2 porous material, was synthesized using a two-step procedure. The material was characterized, and its bimodal porosity and efficient enzyme activities were observed. The study also proposed reaction mechanisms and demonstrated the role of silica in catalase activity.
Article
Veterinary Sciences
Rejane Santos Sousa, Caroline Santos Sousa, Francisco Leonardo Costa Oliveira, Paulo Ricardo Firmino, Isadora Karolina Freitas Sousa, Valeria Veras Paula, Nohora Mercado Caruso, Enrico Lippi Ortolani, Antonio Humberto Hamad Minervino, Raimundo Alves Barreto-Junior
Summary: Blood loss in sheep can cause clinical, hematological, blood gas, and biochemical alterations, leading to oxidative stress and reduced antioxidant activity.
VETERINARY SCIENCES
(2022)