Article
Biochemical Research Methods
Xinyuan He, Tianyu Gao, Yan Chen, Kun Liu, Jiantao Guo, Wei Niu
Summary: In this study, we successfully achieved genetic code expansion in Pseudomonas putida KT2440, introducing non-natural amino acids into proteins. By optimizing the decoding system, we successfully incorporated four non-natural amino acids into proteins and demonstrated the utility of genetic code expansion in protein-protein interaction studies.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Kevin J. McNaught, Eugene Kuatsjah, Michael Zahn, Erica T. Prates, Huiling Shao, Gayle J. Bentley, Andrew R. Pickford, Josephine N. Gruber, Kelley V. Hestmark, Daniel A. Jacobson, Brenton C. Poirier, Chen Ling, Myrsini San Marchi, William E. Michener, Carrie D. Nicora, Jacob N. Sanders, Caralyn J. Szostkiewicz, Dusan Velickovic, Mowei Zhou, Nathalie Munoz, Young-Mo Kim, Jon K. Magnuson, Kristin E. Burnum-Johnson, K. N. Houk, John E. McGeehan, Christopher W. Johnson, Gregg T. Beckham
Summary: Deciphering the mechanisms of bacterial fatty acid biosynthesis is crucial for both engineering bacterial hosts and developing antibiotics. This study reveals that Pseudomonas putida KT2440 has three distinct pathways for initiating fatty acid biosynthesis, with the third pathway being a novel alternative. The findings provide new opportunities for various biotechnological and biomedical applications.
METABOLIC ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Paulius Matulis, Ingrida Kutraite, Ernesta Augustiniene, Egle Valanciene, Ilona Jonuskiene, Naglis Malys
Summary: Indole is a biologically active compound found in plants and bacteria, with various applications in pharmaceuticals, chemicals, and agriculture. This study focuses on the identification and characterization of the indole-inducible system in Pseudomonas putida KT2440 through genetically encoded biosensors. The results provide a basis for using whole-cell biosensors in screening and characterization of indole metabolism-related bacterial species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Si Liu, Tanja Narancic, Jia-Lynn Tham, Kevin E. E. O'Connor
Summary: Through studying Pseudomonas putida KT2440, it was found that PhaJ4 is the main supplier of monomers for PHA synthesis, while PhaJ1 has preference for C6 substrate. Even when all PhaJ homologues were deleted, the mutant still accumulated PHA. Further proteome analysis revealed PedE and PedH as potential monomer suppliers, but even with their deletion, PHA levels remained unchanged. Therefore, PHA metabolism exhibits redundancy.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Agricultural Engineering
Jin Li, Bang-Ce Ye
Summary: In this study, a method for biosynthesis of protocatechuic acid (PCA) in Pseudomonas putida KT2440 was developed with high yield. By using constitutive promoters and metabolic engineering strategies, the engineered strain achieved the highest reported titer of PCA from glucose. Further bioprocess engineering efforts led to significant improvements in PCA production, demonstrating the potential for metabolically engineered microbial cell factories.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yiting Zhang, Honglu Liu, Yujie Liu, Kaiyue Huo, Shufang Wang, Ruihua Liu, Chao Yang
Summary: This study successfully increased the PHA yield by screening endogenous strong promoters from P. putida KT2440, demonstrating the potential of this strategy for enhanced production of secondary metabolites and heterologous proteins in P. putida KT2440.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Agricultural Engineering
Saddam Hussain Mohammad, Bhima Bhukya
Summary: This study successfully utilized Pseudomonas putida KT2440 to valorize lignin and its derivatives, and synthesized bio-polymers without any genetic manipulation.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Arslan Sarwar, Linh Thanh Nguyen, Eun Yeol Lee
Summary: This study demonstrates the efficient upgrading of ethanol to fatty acid ethyl esters (FAEEs) using Pseudomonas putida KT2440, achieving high yields through gene expression and culture optimization. It provides a basis for integrating bioethanol production with the efficient conversion of ethanol to biodiesel.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Zhangyang Xu, Chunmei Pan, Xiaolu Li, Naijia Hao, Tong Zhang, Matthew J. Gaffrey, Yunqiao Pu, John R. Cort, Arthur J. Ragauskas, Wei-Jun Qian, Bin Yang
Summary: Co-feeding lignin derivatives with glycerol was shown to increase cell dry weight and PHA content in P. putida KT2440. The addition of lignin derivatives altered the distribution of PHA monomers and benzoate acted as an independent factor in PHA synthesis. Proteomics and metabolomics analysis revealed that benzoate stimulated oxidative-stress responses and altered intracellular NADH/NADPH ratios, increasing energy generation and storage processes for enhanced PHA biosynthesis.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Biotechnology & Applied Microbiology
Hyun Gyu Lim, Kevin Rychel, Anand Sastry, Gayle J. Bentley, Joshua Mueller, Heidi S. Schindel, Peter E. Larsen, Philip D. Laible, Adam M. Guss, Wei Niu, Christopher W. Johnson, Gregg T. Beckham, Adam M. Feist, Bernhard O. Palsson
Summary: This study applies a machine-learning approach to uncover the genome-scale transcriptional regulatory network in Pseudomonas putida KT2440, providing a basis for understanding its physiological processes and engineering it for practical use.
METABOLIC ENGINEERING
(2022)
Article
Microbiology
Tania Henriquez, Heinrich Jung
Summary: The MxtR/ErdR system is essential for acetate utilization in Pseudomonas putida KT2440, stimulating the expression of specific genes, but does not play a role in regulating siderophore production and the expression of RND transporters.
Article
Chemistry, Multidisciplinary
Henrik Almqvist, Henrique Veras, Kena Li, Javier Garcia Hidalgo, Christian Hulteberg, Marie Gorwa-Grauslund, Nadia Skorupa Parachin, Magnus Carlquist
Summary: This study developed a process for depolymerization of industrial lignin and conversion of guaiacol to muconic acid, providing an effective pathway for bioconversion.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Philipp Demling, Andreas Ankenbauer, Bianca Klein, Stephan Noack, Till Tiso, Ralf Takors, Lars M. Blank
Summary: The study demonstrated that Pseudomonas putida KT2440 can withstand repeated oxygen limitations encountered in large-scale bioreactors, with slowed growth and production but maintained stability. Intracellular ATP concentrations can be restored, and minimal changes in the proteome were observed under oscillations in dissolved oxygen tensions.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Environmental Sciences
Yuxin Zhao, You Che, Fang Zhang, Jiacheng Wang, Weixia Gao, Tong Zhang, Chao Yang
Summary: In this study, an efficient pathway construction strategy for integrating synthetic biodegradation pathways into the chromosome of Pseudomonas putida KT2440 was developed. The strategy resulted in improved chromosomal integration efficiencies and highlighted the potential for in situ bioremediation. Additionally, the strategy can be expanded to a broad range of bacterial species for widespread applications in bioremediation.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Agricultural Engineering
Jin Li, Cheng Yue, Wenping Wei, Yanzhe Shang, Ping Zhang, Bang-Ce Ye
Summary: This study successfully developed an auto-regulatory system to address the challenging issues in lignin valorization, demonstrating its effectiveness and economic advantages.
BIORESOURCE TECHNOLOGY
(2022)
Article
Plant Sciences
Kevin Royet, Nicolas Parisot, Agnes Rodrigue, Erwan Gueguen, Guy Condemine
MOLECULAR PLANT PATHOLOGY
(2019)
Article
Plant Sciences
P. Ragavan, Sourav Kumar Dubey, Muktipada Panda, Malancha Deyroy, K. Ravichandran, Raman K. Trivedi, R. S. C. Jayaraj, P. M. Mohan, T. S. Rana
NORDIC JOURNAL OF BOTANY
(2019)
Article
Genetics & Heredity
Madhavi Mangalarapu, Swetha Vinukonda, Prasanna Latha Komaravalli, Praveen Nagula, Rekha Rani Koduganti, Premsagar Korripally, Someswar Rao Sagurthi
Article
Environmental Sciences
Mounia Kassa-Laouar, Aicha Mechakra, Agnes Rodrigue, Ouissem Meghnous, Alima Bentellis, Oualida Rached
POLISH JOURNAL OF ENVIRONMENTAL STUDIES
(2020)
Article
Microbiology
Julien Cayron, Geraldine Effantin, Elsa Prudent, Agnes Rodrigue
RESEARCH IN MICROBIOLOGY
(2020)
Article
Multidisciplinary Sciences
Lulu Liu, Virginie Gueguen-Chaignon, Isabelle R. Goncalves, Christine Rascle, Martine Rigault, Alia Dellagi, Elise Loisel, Nathalie Poussereau, Agnes Rodrigue, Laurent Terradot, Guy Condemine
NATURE COMMUNICATIONS
(2019)
Article
Ecology
V. Sachithanandam, P. Parthasarathy, S. Sai Elangovan, K. Kasilingam, P. Dhivya, T. Mageswaran, P. M. Mohan
REGIONAL STUDIES IN MARINE SCIENCE
(2020)
Article
Green & Sustainable Science & Technology
Katharine A. Owens, Jaya Divakaran Sarasamma, Katie Conlon, Solomon Kiruba, Alwyn Biju, Niyathi Vijay, Manikandan Subramanian, Smitha Asok Vijayamma, Ayona Jayadev, Vineeta Hoon, Rebekah Padgett, Pranoti Joshi Khanolkar, Dilip K. Kakavipure, P. M. Mohan, Sourav Chattopadhyay, Chinmay Khanolkar
Summary: This article provides an overview of the literature on marine debris in India and introduces a replicable and inexpensive collection method. The authors share baseline data from ten cleanups using this method, which reveal that plastic is the most frequently found material. The study also ranks the cleanliness of different collection sites using the Clean Coast Index. However, the replication of the method among practitioners in other regions of India falls below target.
Article
Ecology
V. Sachithanandam, P. M. Mohan, R. Karthik, S. Sai Elangovan, T. Mageswaran, N. Marimuthu
Summary: This study conducted a year-round assessment on phytoplankton diversity and abundance in the coastal waters of the Andaman Islands. The results showed variations in phytoplankton diversity and abundance across different seasons and regions, influenced by environmental factors such as temperature, salinity, and nitrite.
REGIONAL STUDIES IN MARINE SCIENCE
(2022)
Article
Environmental Sciences
V. Soumya, H. Basira, P. Kiranmayi
Summary: This study evaluated the potential of Catharanthus roseus to remediate industrially contaminated soils for the first time. The results showed that C. roseus was effective in remediating contaminated soils and exhibited enhanced antioxidant potential and increased chlorophyll, pectin, and lignin content in response to metal stress.
INTERNATIONAL JOURNAL OF PHYTOREMEDIATION
(2023)
Article
Environmental Sciences
P. M. Mohan, Shivam Tiwari, Murugan Karuvelan, Sumathi Malairajan, T. Mageswaran, V. Sachithanandam
Summary: Microplastic has become a persistent contaminant globally, prevalently found in terrestrial, marine, and atmospheric environments. This study focuses on recording the prevalence of microplastic on the tourist beaches of Port Blair, Andaman and Nicobar Islands. Different forms of microplastics, such as lines, fragments, pellets, foams, and fibers, were found at the sampled sites. The highest concentration of microplastic particles was observed at Wandoor beach, while Quarry beach near the municipal waste dumpsite had the lowest concentration. The existence of a new type of polymer, plasta zinc, possibly indicating nanoplastic and biological enzymatic degradation of microplastic, was revealed through Fourier Transform Infrared spectroscopy (FTIR).
MARINE POLLUTION BULLETIN
(2022)
Article
Environmental Sciences
A. A. Fousiya, G. H. Aravind, H. Achyutan, S. Chakraborty, R. Chattopadhyay, A. Datye, C. Murkute, A. M. Lone, R. H. Kripalani, M. G. Yadava, P. M. Mohan
Summary: The isotopic values of rainfall in southern India show strong seasonal variations, which are mainly influenced by the reversal of monsoon winds and atmospheric processes. The study reveals that oxygen isotopes in the marine environment respond differently to temperature compared to those in the land region, and the seasonal isotopic gradient in the marine environment is modulated by the tropospheric temperature anomaly. The oxygen isotopic values are strongly associated with the Webster-Yang monsoon index, indicating the potential of using precipitation isotopes to study monsoon processes. The evaluation of the potential of precipitation isotopic records in characterizing the thermodynamical properties of the atmosphere and estimating the summer monsoon withdrawal phase is also an important outcome of this study.
WATER RESOURCES RESEARCH
(2022)
Article
Environmental Sciences
Ed C. Hathorne, Martin Frank, P. M. Mohan
FRONTIERS IN MARINE SCIENCE
(2020)
Article
Multidisciplinary Sciences
Nitesh Sinha, S. Chakraborty, P. M. Mohan
Article
Genetics & Heredity
Gugalavath Shailender, Seema Kumari, Patnala Kiranmayi, Rama Rao Malla
GENES AND ENVIRONMENT
(2019)