Article
Plant Sciences
Peng Li, Xiulan Cao, Liwen Zhang, Mingfa Lv, Lian-Hui Zhang
Summary: This study demonstrated the important role of ralsolamycin in the crosstalk between R. solanacearum and fungi. Ralsolamycin biosynthesis is regulated by multiple factors, and the transcription factors PhcA and PhcR were found to bind to the promoter of the rmy operon and modulate ralsolamycin production in opposite ways.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Microbiology
Xiaojing Fan, Zhiwen Zhao, Tingyan Sun, Wei Rou, Caiying Gui, Tahira Saleem, Xuxu Zhao, Xianyuan Xu, Tao Zhuo, Xun Hu, Huasong Zou
Summary: The study reveals that the regulator CrgA in Ralstonia solanacearum suppresses cell motility by repressing flhDC expression, which consequently affects the expression of fliC to modulate disease development in host plants. This indicates a complex regulation of motility in R. solanacearum and enhances understanding of flagellum regulation in this pathogen.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Microbiology
Qingmei Liu, Chuhao Li, Xiaohan Zhang, Mengfan Ding, Xinyue Liao, Jinli Yan, Ming Hu, Leilei Yang, Xiaoqing Wang, Lisheng Liao, Peng Li, Xiaofan Zhou
Summary: This study identifies a previously uncharacterized response regulator, PhcX, in Ralstonia solanacearum and uncovers its essential functions in modulating virulence and metabolism. The findings contribute to a better understanding of the pathogen's regulatory network and shed light on the evolution of its complex regulatory mechanisms.
Article
Biochemistry & Molecular Biology
Lei Wang, Yang Gao, Nihao Jiang, Jian Yan, Weipeng Lin, Kunzheng Cai
Summary: This study investigated the impact of silicon on the control of bacterial wilt in tomatoes and elucidated the molecular mechanisms involved. The results showed that silicon application significantly reduced the occurrence of bacterial wilt and regulated the expression of virulence-related genes in R. solanacearum.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ansar Hussain, Liu Kaisheng, Ali Noman, Muhammad Furqan Ashraf, Mohammed Albaqami, Muhammad Ifnan Khan, Zhiqin Liu, Shuilin He
Summary: In this study, the functional role of CaASHH3 protein in plant immunity was characterized in pepper. It was found that CaASHH3 acts as a positive regulator in cell death and pepper immunity against bacterial pathogens, and its function is regulated by signals mediated by SA, JA, ET, and ABA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Yutong Zheng, Shicong He, Weiwei Cai, Lei Shen, Xueying Huang, Sheng Yang, Y. U. Huang, Qiaoling Lu, Hui Wang, Deyi Guan, Shuilin He
Summary: The APETALA2 (AP2) subfamily transcription factor CaAIL1 enhances pepper immunity against Ralstonia solanacearum infection by repressing a subset of negative immune regulators during the immune response.
PLANT AND CELL PHYSIOLOGY
(2021)
Review
Microbiology
Kenji Kai
Summary: Ralstonia solanacearum species complex (RSSC) strains are devastating plant pathogens distributed worldwide. The primary cell density-dependent gene expression system in RSSC strains is phc quorum sensing (QS), which regulates the expression of about 30% of all genes. The specificity in generating and receiving its QS signal, but their signaling pathways might not differ significantly. This review discusses the genetic and biochemical factors involved in QS signal input, the regulatory network, and the interactions with soil fungi.
ANNUAL REVIEW OF MICROBIOLOGY
(2023)
Article
Microbiology
Shihao Song, Xiuyun Sun, Quan Guo, Binbin Cui, Yu Zhu, Xia Li, Jianuan Zhou, Lian-Hui Zhang, Yinyue Deng
Summary: This study reveals that anthranilic acid from Ralstonia solanacearum controls bacterial physiology and pathogenicity by regulating the transcriptional regulator RaaR, with the system being widespread in various bacterial species.
Article
Multidisciplinary Sciences
Hui Lin, Muyang Wang, Ying Chen, Kinya Nomura, Shugang Hui, Jinshan Gui, Xiawei Zhang, Yue Wu, Jiyun Liu, Qun Li, Yiwen Deng, Laigeng Li, Meng Yuan, Shiping Wang, Sheng Yang He, Zuhua He
Summary: This study identifies a vascular defense mechanism in plants against bacterial pathogens and reveals a tissue-specific defense regulatory network.
Article
Plant Sciences
Jeffrey K. Schachterle, Qi Huang
Summary: This study investigated the cool-virulence mechanisms of Ralstonia solanacearum UW551 by generating transposon mutants and identifying genes related to reduced virulence at cooler temperatures. The effector gene RipS1 was found to play a role in cool-virulence and could potentially serve as a target for diagnostic tools specific to cool-virulent strains of R. solanacearum. These findings contribute to a better understanding of cool-virulence and the development of control strategies against this plant pathogen.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Wakana Senuma, Masayuki Tsuzuki, Chika Takemura, Yuki Terazawa, Kanako Inoue, Akinori Kiba, Kouhei Ohnishi, Kenji Kai, Yasufumi Hikichi
Summary: After infecting tomato plants, the bacterium Ralstonia pseudosolanacearum strain OE1-1 activates quorum sensing to induce the production of plant cell wall-degrading enzymes and invades xylem vessels to cause disease. A phcA-deletion mutant shows loss of virulence and inability to infect xylem vessels. A cbhA-deletion mutant also lacks the ability to infect xylem vessels and exhibits reduced virulence. Our study reveals that CbhA is involved in the full expression of phcA, contributing to the virulence of strain OE1-1.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Yuyan An, Jialan Chen, Zhangyan Xu, Xue Ouyang, Peng Cao, Rongbo Wang, Peiqing Liu, Meixiang Zhang
Summary: Ralstonia solanacearum is a pathogen that causes devastating diseases in economically important crops. The study shows that RipTPS(G), a type III effector, specifically induces cell death in Nicotiana tabacum, while RipTPS(C) cannot. The expression of RipTPS(G) leads to upregulation of hypersensitive response-related genes in N. tabacum and reduces the virulence of CQPS-1. It is also found that RipTPS contributes to pathogen virulence.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Jinli Yan, Peng Li, Xiaoqing Wang, Minya Zhu, Hongyu Shi, Guohui Yu, Xuemei Chen, Huishan Wang, Xiaofan Zhou, Lisheng Liao, Lianhui Zhang
Summary: In this study, a new functional QS system (RasI/R) was identified and characterized in R. solanacearum strain EP1, which plays an important role in regulating bacterial virulence. This QS system produces and responds to the QS signal 3-OH-C12-HSL and regulates critical bacterial abilities in survival and infection. These findings provide new insight into the complicated QS regulatory networks of R. solanacearum, and offer potential targets for controlling and preventing bacterial wilt diseases.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Rekha Gopalan-Nair, Marie-Francoise Jardinaud, Ludovic Legrand, David Landry, Xavier Barlet, Celine Lopez-Roques, Celine Vandecasteele, Olivier Bouchez, Stephane Genin, Alice Guidot
Summary: The study utilized experimental evolution to investigate the adaptive potential of Ralstonia solanacearum in overcoming the resistance of tomato cultivar Hawaii 7996. After over 300 generations of serial passaging, pathogen adaptation to the resistant cultivar's environment was observed without breakdown of plant resistance. Genomic and transcriptomic analyses revealed genetic alterations and convergent global rewiring of the virulence regulatory network in independently evolved clones.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Plant Sciences
Bingsen Wang, Mengshu Huang, Wenfeng He, Yuqi Wang, Liu Yu, Dan Zhou, Chengzhen Meng, Dong Cheng, Huishan Qiu, Xiaodan Tan, Botao Song, Huilan Chen
Summary: Knock down of StTOPP6 enhances resistance to bacterial wilt in potato by activating the MAPK3-mediated pathway and inducing accumulation of reactive oxygen species.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
