Article
Infectious Diseases
Jonas Bredtoft Boel, Valeria Antsupova, Jenny Dahl Knudsen, Jens Otto Jarlov, Magnus Arpi, Barbara Juliane Holzknecht
Summary: The study suggests that IV mecillinam may be a suitable targeted treatment for bacteraemia with a urinary tract focus, but these results need confirmation by randomized controlled studies.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Chemistry, Multidisciplinary
James A. Martin, Arthur G. Palmer
Summary: This study reveals the kinetic model of the handle region in the ribonuclease HI homologs, including transitions between different conformations. The research findings show that the homologs containing asparagine 88 have distinct structural features compared to those containing arginine or lysine 88.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Microbiology
Kun Li, Zhongping Yang, Jing Gu, Ming Luo, Jiaoyu Deng, Yaokai Chen
Summary: This study identified and analyzed pncA gene mutations in Mycobacterium tuberculosis isolates from Chongqing, China, revealing 124 mutation types, including 30 new mutations. By analyzing mutations and pyrazinamidase activity, 322 isolates out of 424 drug-resistant isolates were predicted to be resistant to PZA.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Cell Biology
Timothy Kudinha, Fanrong Kong
Summary: The prevalence of ST131, especially among ciprofloxacin resistant isolates, increased from fecal to clinical samples in women, men, and children. ST131 isolates showed higher virulence potential compared to non-ST131 isolates, particularly in urinary samples. There was a strong association between ST131 prevalence and fluoroquinolone resistance, with FQ resistant ST131 isolates harboring more virulence genes.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Microbiology
Vidar Sorum, Emma L. Oynes, Anna S. Moller, Klaus Harms, Orjan Samuelsen, Nicole L. Podnecky, Pal J. Johnsen
Summary: This study investigates the stability of collateral networks in ciprofloxacin-resistant Escherichia coli strains. The results show that the collateral effects caused by resistance development are not stable, complicating the general applicability and clinical implementation of collateral effects in treatment strategies.
Article
Multidisciplinary Sciences
Habiba Tariq, Zobia Noreen, Aftab Ahmad, Laraib Khan, Mashhood Ali, Muhammad Malik, Aneela Javed, Faisal Rasheed, Alina Fatima, Tanil Kocagoz, Ugur Sezerman, Habib Bokhari
Summary: This study investigated the genetic diversity of pathogenic E. coli strains associated with colorectal cancer (CRC). It found that 43.47% of the cancer-associated isolates belonged to phylogroup B2, showed resistance to multiple antibiotics, and exhibited cytotoxic activity that may contribute to the progression of CRC.
Article
Microbiology
Katerina Tsilipounidaki, Zoi Florou, Anargyros Skoulakis, George C. C. Fthenakis, Vivi Miriagou, Efthymia Petinaki
Summary: The present study genetically characterized ten NDM-1 producing Escherichia coli isolates from a hospital in Central Greece between 2017 and 2021. Whole genome sequencing was used to determine the multi-locus sequencing typing (MLST), bla(NDM1)-environment, resistome, and plasmid content. The isolates showed a diverse range of sequence types and carried multiple resistance genes to beta-lactams and other classes of antibiotics. The analysis also revealed the presence of various plasmids, indicating continuous horizontal gene transfer among the isolates.
Article
Veterinary Sciences
Vera Fernandes, Eva Cunha, Telmo Nunes, Elisabete Silva, Luis Tavares, Luisa Mateus, Manuela Oliveira
Summary: This study aimed to determine the susceptibility profile of E. coli isolates obtained from healthy and pyometra-presenting dogs and compare the application of different antimicrobial susceptibility guidelines. The results emphasize the importance of determining the antimicrobial susceptibility profile in veterinary isolates and the necessity of using standardized and validated testing methods and harmonized interpretive criteria.
VETERINARY SCIENCES
(2022)
Article
Immunology
Qiaoling Li, Changrui Qian, Xueya Zhang, Tingting Zhu, Weina Shi, Mengdi Gao, Chunlin Feng, Ming Xu, Hailong Lin, Li Lin, Junwan Lu, Xi Lin, Kewei Li, Teng Xu, Qiyu Bao, Changchong Li, Hailin Zhang
Summary: This study investigated the colistin resistance mechanism of clinical E. coli isolates using the agar dilution method, polymerase chain reaction, and comparative genomic analysis. The results showed an increase in colistin resistance in E. coli isolates in 2019 compared to previous years. The study also identified six isolates carrying the mcr-1 gene, including a novel variant with a mutation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Jose Vazquez-Villanueva, Karina Vazquez, Ana Veronica Martinez-Vazquez, Alfredo Wong-Gonzalez, Jesus Hernandez-Escareno, Omar Cabrero-Martinez, Wendy Lizeth Cruz-Pulido, Abraham Guerrero, Gildardo Rivera, Virgilio Bocanegra-Garcia
Summary: This study analyzed 336 samples from a bovine slaughterhouse and found that 83.3% of the samples were positive for E. coli infection. The study revealed that these E. coli isolates exhibited resistance to multiple antibiotics and carried various virulence factors and resistance genes. Therefore, E. coli poses a threat to public health.
Article
Veterinary Sciences
Nurul Asyiqin Haulisah, Latiffah Hassan, Siti Khairani Bejo, Saleh Mohammed Jajere, Nur Indah Ahmad
Summary: The overuse of antimicrobials in livestock has been recognized as a major risk factor for the acceleration of antimicrobial resistance. A study found high levels of resistance in clinical isolates from both ruminant and non-ruminant animals, with a significant portion of isolates being multi-drug resistant.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Microbiology
Guilhem Royer, Jose-Manuel Ortiz de La Rosa, Xavier Vuillemin, Beatrice Lacombe, Francoise Chau, Olivier Clermont, Melanie Mercier-Darty, Jean-Winoc Decousser, Jean-Damien Ricard, Patrice Nordmann, Erick Denamur, Laurent Poirel
Summary: Chlorhexidine is a widely used antiseptic, but there is a growing concern about decreased susceptibility to this compound in bacteria. This study investigated the resistance mechanisms of Escherichia coli to chlorhexidine through whole-genome sequence analysis. The results showed that two different evolutionary paths lead to chlorhexidine decreased susceptibility, one involving a retrograde phospholipid transport system and the other associated with efflux pump TetA.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Biotechnology & Applied Microbiology
Reed Woyda, Adelumola Oladeinde, Zaid Abdo
Summary: Pathogenic Escherichia coli causes disease in humans and food-producing animals. Its pathogenesis depends on virulence factors and antimicrobial resistance genes. Contaminated and undercooked food products are highly associated with food-borne outbreaks. It is important to identify the genetic factors that contribute to the virulence and antimicrobial resistance of E. coli.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Infectious Diseases
Kazuma Sakaeda, Takuya Sadahira, Yuki Maruyama, Takehiro Iwata, Masami Watanabe, Koichiro Wada, Motoo Araki
Summary: We conducted a molecular biological analysis of ESBL-producing E. coli strains and their sensitivity to FMOX. Most of the strains belonged to the ST-131 sequence type and had CTX-M genotypes. The isolated strains were sensitive to FMOX in drug susceptibility tests. FMOX showed efficacy for perioperative prophylactic administration in urologic surgery and for therapeutic administration for urinary tract infections.
Article
Food Science & Technology
Emmanuel W. Bumunang, Rahat Zaheer, Kim Stanford, Chad Laing, Dongyan Niu, Le Luo Guan, Linda Chui, Gillian A. M. Tarr, Tim A. McAllister
Summary: This study characterized the presence of Shiga toxin-producing Escherichia coli (STEC) O157 in cattle and clinical samples through a comparative genomic analysis. It was found that most isolates carried virulence genes stx1, stx2, eae, and hlyA, with variations in stx-phage insertion sites and antimicrobial resistance genes between cattle and clinical strains.
Article
Immunology
Karin Hjort, Pontus Juren, Juan Carlos Toro, Sven Hoffner, Dan Andersson, Linus Sandegren
Summary: Whole-genome sequencing of Mycobacterium tuberculosis from a single patient over 9 years of disease and treatment revealed that antibiotic treatment was the main driver of sequence evolution, leading to stepwise accumulation of resistance mutations to various antibiotics. The study highlighted the dynamic nature of M. tuberculosis infection, population structure, and resistance evolution, emphasizing the importance of rapid antibiotic susceptibility tests in combating this pathogen.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Microbiology
Clara Atterby, Marie Nykvist, Ulrika Lustig, Dan Andersson, Josef D. Jarhult, Linus Sandegren
Summary: Exposure to sub-MIC levels of ciprofloxacin can lead to enrichment of Escherichia coli with reduced susceptibility to ciprofloxacin in wildlife, highlighting the potential impact of antibiotic pollution on the environment. The study found that in vivo selection of gyrA mutants occurred in mallards during exposure to ciprofloxacin concentrations found in aquatic environments, with resistance plasmids readily transferring between strains in the intestines of the mallards. Ultimately, the findings underscore the importance of reducing antibiotic contamination to curb the spread of resistance.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Genetics & Heredity
Michael Knopp, Arianne M. Babina, Jonina S. Gudmundsdottir, Martin Douglass, M. Stephen Trent, Dan Andersson
Summary: Random sequences can generate novel antibiotic resistance determinants de novo, which act via specific peptide-protein interactions in the transmembrane domain. These peptides may potentially evolve as bona fide resistance determinants in natura.
Article
Infectious Diseases
Jessica Howard-Anderson, Michelle Davis, Alexander M. Page, Chris W. Bower, Gillian Smith, Jesse T. Jacob, Dan Andersson, David S. Weiss, Sarah W. Satola
Summary: This study investigated the prevalence of colistin heteroresistance in carbapenem-resistant Pseudomonas aeruginosa (CRPA) and its association with clinical outcomes. The results showed that colistin heteroresistance was present in up to 26% of CRPA patients, but it did not have a significant impact on 90-day mortality.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Immunology
Greta Zaborskyte, Erik Wistrand-Yuen, Karin Hjort, Dan I. Andersson, Linus Sandegren
Summary: Medical device-related biofilms are a major cause of hospital-acquired infections. The 3D-printed device (FlexiPeg) presented in this study is a robust and easy-to-use tool for studying biofilms, with versatile applications.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Food Science & Technology
Konstantinos Koutsoumanis, Ana Allende, Avelino Alvarez-Ordonez, Declan Bolton, Sara Bover-Cid, Marianne Chemaly, Robert Davies, Alessandra De Cesare, Lieve Herman, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Dan Andersson, Vasileios Bampidis, Johan Bengtsson-Palme, Damien Bouchard, Aude Ferran, Maryline Kouba, Secundino Lopez Puente, Marta Lopez-Alonso, Soren Saxmose Nielsen, Alena Pechova, Mariana Petkova, Sebastien Girault, Alessandro Broglia, Beatriz Guerra, Matteo Lorenzo Innocenti, Ernesto Liebana, Gloria Lopez-Galvez, Paola Manini, Pietro Stella, Luisa Peixe
Summary: The study assessed specific concentrations of tilmicosin, tylosin, and tylvalosin in feed for food-producing animals, and their impact on bacterial resistance and growth promotion. The Feed Antimicrobial Resistance Selection Concentration (FARSC) model was used for the assessment of antimicrobial resistance, while scientific publications were utilized for growth promotion evaluation. Additional studies are recommended to fill the data gaps for tylvalosin.
Article
Food Science & Technology
Konstantinos Koutsoumanis, Ana Allende, Avelino Alvarez-Ordonez, Declan Bolton, Sara Bover-Cid, Marianne Chemaly, Robert Davies, Alessandra De Cesare, Lieve Herman, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Dan Andersson, Vasileios Bampidis, Johan Bengtsson-Palme, Damien Bouchard, Aude Ferran, Maryline Kouba, Secundino Lopez Puente, Marta Lopez-Alonso, Soren Saxmose Nielsen, Alena Pechova, Mariana Petkova, Sebastien Girault, Alessandro Broglia, Beatriz Guerra, Matteo Lorenzo Innocenti, Ernesto Liebana, Gloria Lopez-Galvez, Paola Manini, Pietro Stella, Luisa Peixe
Summary: The study assessed the specific concentrations of four tetracyclines in feed for food-producing animals, as well as their effects on bacterial resistance and growth promotion. The FARSC model and data from scientific publications were used for the assessment, highlighting uncertainties and data gaps. Further studies were recommended to provide more diverse and complete data for calculation of the FARSC for these antimicrobials.
Article
Food Science & Technology
Konstantinos Koutsoumanis, Ana Allende, Avelino Alvarez-Ordonez, Declan Bolton, Sara Bover-Cid, Marianne Chemaly, Robert Davies, Alessandra De Cesare, Lieve Herman, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Dan Andersson, Vasileios Bampidis, Johan Bengtsson-Palme, Damien Bouchard, Aude Ferran, Maryline Kouba, Secundino Lopez Puente, Marta Lopez-Alonso, Soren Saxmose Nielsen, Alena Pechova, Mariana Petkova, Sebastien Girault, Alessandro Broglia, Beatriz Guerra, Matteo Lorenzo Innocenti, Ernesto Liebana, Gloria Lopez-Galvez, Paola Manini, Pietro Stella, Luisa Peixe
Summary: EFSA, in collaboration with EMA, assessed specific concentrations of amprolium in non-target feed and specific antimicrobial concentrations in feed for growth promotion. The assessment was based on the Feed Antimicrobial Resistance Selection Concentration (FARSC) model, but a lack of data prevented a conclusion on antimicrobial resistance. Data from scientific publications were used to report levels of amprolium in feed affecting growth promotion.
Article
Food Science & Technology
Konstantinos Koutsoumanis, Ana Allende, Avelino Alvarez-Ordonez, Declan Bolton, Sara Bover-Cid, Marianne Chemaly, Robert Davies, Alessandra De Cesare, Lieve Herman, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Dan I. Andersson, Vasileios Bampidis, Johan Bengtsson-Palme, Damien Bouchard, Aude Ferran, Maryline Kouba, Secundino Lopez Puente, Marta Lopez-Alonso, Soren Saxmose Nielsen, Alena Pechova, Mariana Petkova, Sebastien Girault, Alessandro Broglia, Beatriz Guerra, Matteo Lorenzo Innocenti, Ernesto Liebana, Gloria Lopez-Galvez, Paola Manini, Pietro Stella, Luisa Peixe
Summary: EFSA and EMA collaborated to assess the specific concentrations of sulfonamides in non-target feed for food-producing animals and their impact on bacterial resistance and growth promotion. However, due to lack of data, conclusions could not be drawn, and further studies are needed to fill the gaps.
Article
Food Science & Technology
Konstantinos Koutsoumanis, Ana Allende, Avelino Alvarez-Ordonez, Declan Bolton, Sara Bover-Cid, Marianne Chemaly, Robert Davies, Alessandra De Cesare, Lieve Herman, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Dan Andersson, Vasileios Bampidis, Johan Bengtsson-Palme, Damien Bouchard, Aude Ferran, Maryline Kouba, Secundino Lopez Puente, Marta Lopez-Alonso, Soren Saxmose Nielsen, Alena Pechova, Mariana Petkova, Sebastien Girault, Alessandro Broglia, Beatriz Guerra, Matteo Lorenzo Innocenti, Ernesto Liebana, Gloria Lopez-Galvez, Paola Manini, Pietro Stella, Luisa Peixe
Summary: The EFSA collaborated with the EMA to assess specific concentrations of amoxicillin and penicillin V in non-target feed for food-producing animals. The study aimed to understand the effects on bacterial resistance and growth promotion. New assessment models were recommended for addressing antimicrobial resistance, along with the need for further data to fill the gaps in calculations.
Review
Microbiology
Roderich Roemhild, Tobias Bollenbach, Dan I. Andersson
Summary: This review discusses the importance of using combination antibiotics to enhance treatment efficacy and combat resistance evolution. While several promising strategies have been proposed, a deeper understanding of the physiological responses and genetic mechanisms underlying antibiotic interactions and their clinical applicability is still lacking.
NATURE REVIEWS MICROBIOLOGY
(2022)
Article
Immunology
Po-Cheng Tang, Olle Eriksson, Josefin Sjogren, Nikos Fatsis-Kavalopoulos, Johan Kreuger, Dan I. Andersson
Summary: Biofilms are a crucial mode of growth for bacteria, but how antibiotic resistance emerges and is selected in biofilms is poorly understood. This study developed and validated a microfluidic chip (Brimor) for studying the dynamics of antibiotic-resistant bacteria in biofilms. The chip was found to be easy to use and a relevant model for studying the selection of antibiotic resistance in bacterial biofilms, providing valuable insights into this area of research.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Karin Hjort, Elin Fermer, Po-Cheng Tang, Dan I. Andersson
Summary: The use and misuse of antibiotics have led to the selection of difficult-to-treat resistant bacteria. This study compared the selection of antibiotic-resistant E. coli strains during planktonic and biofilm growth. The results showed that resistant mutants were selected at sub-minimal inhibitory concentrations (MICs) in both growth conditions, emphasizing the significance of low antibiotic concentrations in driving the emergence and enrichment of resistant bacteria.
Article
Biology
Arianne M. Babina, Serhiy Surkov, Weihua Ye, Jon Jerlstrom-Hultqvist, Marten Larsson, Erik Holmqvist, Per Jemth, Dan Andersson, Michael Knopp
Summary: The origins and evolution of small proteins with diverse physiological roles remain unclear. Recent genomic sequence analyses suggest that new functions encoded by small open reading frames (sORFs) may emerge de novo from noncoding sequences. This study shows that randomly generated sORFs can rescue Escherichia coli cells by upregulating hisB expression and that they bind to the 5' end regulatory region of the his operon mRNA, promoting structural rearrangements of the RNA for increased hisB expression. This provides experimental evidence that small proteins with selective benefits can originate from the expression of nonfunctional sequences.
Article
Biology
Ramith R. Nair, Dan I. Andersson
Summary: The dynamics of nitrofurantoin resistance selection in Escherichia coli is significantly affected by interspecies interactions with Bacillus subtilis. The presence of B. subtilis slows down the selection for resistant E. coli mutants by producing extracellular compounds, particularly the YydF peptide. This study highlights the importance of considering interspecies interactions in understanding and predicting the evolution of antibiotic resistance.
COMMUNICATIONS BIOLOGY
(2023)