Article
Immunology
Patricia J. Simner, Heba H. Mostafa, Yehudit Bergman, Michael Ante, Tsigereda Tekle, Ayomikun Adebayo, Stephan Beisken, Kathryn Dzintars, Pranita D. Tamma
Summary: This study revealed the mechanisms by which New Delhi metallo-β-lactamase-producing Escherichia coli isolate developed relatively rapid resistance to cefiderocol through increased copy numbers of bla(NDM) genes. The findings highlight the importance of investigating increased bla(NDM-5) expression frequency in contributing to cefiderocol resistance.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Immunology
Esther Rios, Maria Del Carmen Lopez Diaz, Esther Culebras, Iciar Rodriguez-Avial, Carmen Rodriguez-Avial
Summary: In this study, the in vitro activity of fosfomycin against Escherichia coli isolated from urine samples in 2013, 2018, and 2021 was evaluated. The results showed an increasing trend of fosfomycin resistance in E. coli urinary isolates, which was associated with extended-spectrum beta-lactamases (ESBL) production.
MEDICAL MICROBIOLOGY AND IMMUNOLOGY
(2022)
Article
Microbiology
Maureen Daly, James Powell, Nuala H. H. O'Connell, Liz Murphy, Colum P. P. Dunne
Summary: High levels of bacterial antimicrobial resistance have been found in environmental studies in Ireland and elsewhere, mainly due to the inappropriate use of antibiotics and the release of residual antibiotics into the environment. This study analyzed enterobacterales from different water sources in Ireland and found a low but significant level of antibiotic resistance, emphasizing the need for ongoing surveillance of drinking water as a potential source of antimicrobial resistance.
Article
Multidisciplinary Sciences
Gabhan Chalmers, Rebecca E. V. Anderson, Roger Murray, Edward Topp, Patrick Boerlin
Summary: The study reveals that P. mirabilis can harbor and transmit ESC resistance genes and plasmids, making it a potential reservoir of antimicrobial resistance.
Article
Microbiology
Steven Dunn, Laura Carrilero, Michael Brockhurst, Alan McNally
Summary: The study found that different E. coli strains vary in their ability to acquire and maintain MDR plasmids, with highly strain-specific transcriptional responses observed following plasmid acquisition. However, the subtle transcriptional responses consistent across all strains suggest that fitness costs arising from transcriptional disruption are unlikely to act as a barrier to dissemination of this MDR plasmid in E. coli.
Article
Infectious Diseases
Bradd J. Haley, Seon Woo Kim, Serajus Salaheen, Ernest Hovingh, Jo Ann S. Van Kessel
Summary: The gastrointestinal tracts of dairy calves and cows are reservoirs of antimicrobial-resistant bacteria. Young calves harbor a greater abundance of resistant bacteria than older cows, but the factors driving this high abundance are unknown. This study aimed to characterize multidrug-resistant (MDR) and antimicrobial-susceptible Escherichia coli strains isolated from different stages of dairy cows and identify accessory genes associated with MDR to discover genetic targets for mitigating antimicrobial resistance on dairy farms.
Article
Multidisciplinary Sciences
Mutasim E. Ibrahim
Summary: This study investigated the prevalence and risk factors for multidrug-resistant Klebsiella pneumoniae infection among hospitalized patients in southern Saudi Arabia. The study found that 66.8% of patients were infected with multidrug-resistant K. pneumoniae strains. Factors such as gender, age, intensive care unit admission, invasive medical devices, and chronic illness were significantly associated with the infection.
SCIENTIFIC REPORTS
(2023)
Article
Immunology
Nadghia F. Leite-Sampaio, Cicera Natalia F. L. Gondim, Celestina E. Sobral de Souza, Henrique D. M. Coutinho
Summary: This study investigated the antibacterial and antimicrobial modulating activity of alpha-pinene and borneol against Escherichia coli and enteropathogenic and enterotoxigenic serotypes. The results showed that alpha-pinene and borneol exhibited low antimicrobial action against multidrug-resistant Escherichia coli, but no antimicrobial activity was observed against enteropathogenic and enterotoxigenic serotypes. A synergistic action of borneol with ciprofloxacin against enterotoxigenic serotypes was noted.
MICROBIAL PATHOGENESIS
(2022)
Review
Infectious Diseases
Lorcan O'Neill, Edgar Garcia Manzanilla, Daniel Ekhlas, Finola C. Leonard
Summary: Antimicrobial resistance in Escherichia coli of animal origin poses a threat to human health. Pig farming is one of the largest livestock sectors and understanding the epidemiology of antimicrobial resistance in E. coli of porcine origin is crucial.
Article
Infectious Diseases
Antonio Mandujano, Diana Veronica Cortes-Espinosa, Jose Vasquez-Villanueva, Paulina Guel, Gildardo Rivera, Karina Juarez-Rendon, Wendy Lizeth Cruz-Pulido, Guadalupe Aguilera-Arreola, Abraham Guerrero, Virgilio Bocanegra-Garcia, Ana Veronica Martinez-Vazquez
Summary: This study investigated the antimicrobial resistance profile of ESBL-producing E. coli among food-producing animals in Tamaulipas, Mexico. A total of 200 fecal samples were collected and 5.0% of the strains were confirmed as ESBL producers. High percentage of antimicrobial resistance was observed against gentamicin, tetracycline, and streptomycin. The study suggests that food-producing animals may serve as reservoirs and contribute to the spread of ESBL-producing bacteria.
Article
Ecology
Maya L. Nadimpalli, Marc Stegger, Roberto Viau, Vuthy Yith, Agathe de Lauzanne, Nita Sem, Laurence Borand, Bich-tram Huynh, Sylvain Brisse, Virginie Passet, Soren Overballe-Petersen, Maliha Aziz, Malika Gouali, Jan Jacobs, Thong Phe, Bruce A. Hungate, Victor O. Leshyk, Amy J. Pickering, Francois Gravey, Cindy M. Liu, Timothy J. Johnson, Simon Le Hello, Lance B. Price
Summary: Antibiotic resistance is a major public health challenge, and efforts to address it have mainly focused on drug development and reducing unnecessary antibiotic use. However, in areas with poor water, sanitation, and hygiene infrastructure, bacterial transmission between humans and animals can worsen the emergence and spread of resistant pathogens. In Cambodia, a middle-income country with high human-animal connectivity and unregulated antibiotic use, identical mobile resistance elements were found in both humans and animals, which is rare in high-income settings. The study suggests that controlling the transmission of resistant bacteria at the human-animal interface is crucial in addressing antibiotic resistance in low- and middle-income countries.
FRONTIERS IN ECOLOGY AND THE ENVIRONMENT
(2023)
Article
Food Science & Technology
Chengtao Sun, Yingchao Wang, Shizhen Ma, Shan Zhang, Dejun Liu, Yang Wang, Congming Wu
Summary: Analysis of food samples collected at retail stores in Beijing, China, showed high contamination levels of E. coli and enterococci in food-animal products, vegetables, aquatic products, and fruits, with significant multidrug resistance. Food-animal products were identified as the primary reservoir of antimicrobial resistance, while the role of vegetables, fruits, and aquatic products in spreading AMR should not be underestimated. Food-borne isolates displayed high resistance to antibiotics commonly used in agriculture, as well as those rarely used in agriculture/aquaculture, highlighting the potential for animal-to-human transmission of antibiotic resistance genes.
Article
Biochemistry & Molecular Biology
Bhishem Thakur, Kanika Arora, Archit Gupta, Purnananda Guptasarma
Summary: Researchers investigated whether the nucleoid-associated histone-like protein (HU) could act as a glue in biofilms, finding evidence through various experiments to support this hypothesis.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Matthew D. Surette, Nicholas Waglechner, Kalinka Koteva, Gerard D. Wright
Summary: Rifamycin antibiotics like rifampin are powerful inhibitors of prokaryotic RNA polymerase, used for treating tuberculosis and bacterial infections. While resistance mainly arises through mutations in RNA polymerase, many bacteria have specific enzyme-mediated resistance mechanisms that modify and inactivate rifamycins. A helicase-like protein called HelR has been identified in Streptomyces venezuelae which confers broad-spectrum rifamycin resistance and also promotes tolerance to these antibiotics, allowing bacteria to evade their toxic properties. HelR forms a complex with RNA polymerase and displaces rifamycins from it, providing resistance by target protection. HelRs are widely distributed in Actinobacteria, including opportunistic mycobacterial pathogens, posing a challenge for developing new rifamycin antibiotics.
Article
Infectious Diseases
Paul Laffont-Lozes, Florian Salipante, Geraldine Leguelinel-Blache, Catherine Dunyach-Remy, Jean-Philippe Lavigne, Albert Sotto, Romaric Larcher
Summary: This study aimed to determine the correlation between antimicrobial consumption (AMC) and antimicrobial resistance (AMR) in Escherichia coli at a hospital level, and evaluate the use of dynamic regression (DR) models for predicting AMR and implementing antimicrobial stewardship programs (ASPs). The study was conducted in a French tertiary hospital between 2014 and 2019. The results showed that rates of fluoroquinolone and cephalosporin resistance decreased, while overall AMC increased but decreased for fluoroquinolone. DR models revealed that changes in antibiotic usage explained a significant portion of the decrease in resistance. However, caution should be exercised when using DR models for AMR forecasting and ASP implementation.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Review
Microbiology
Jeremy C. Henderson, Shawn M. Zimmerman, Alexander A. Crofts, Joseph M. Boll, Lisa G. Kuhns, Carmen M. Herrera, M. Stephen Trent
ANNUAL REVIEW OF MICROBIOLOGY, VOL 70
(2016)
Article
Multidisciplinary Sciences
Joseph M. Boll, Alexander A. Crofts, Katharina Peters, Vincent Cattoir, Waldemar Vollmer, Bryan W. Davies, M. Stephen Trent
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Biochemistry & Molecular Biology
Carmen M. Herrera, Jeremy C. Henderson, Alexander A. Crofts, M. Stephen Trent
MOLECULAR MICROBIOLOGY
(2017)
Article
Microbiology
Gregory A. Knauf, Ashley L. Cunningham, Misha I. Kazi, Ian M. Riddington, Alexander A. Crofts, Vincent Cattoir, M. Stephen Trent, Bryan W. Davies
Article
Microbiology
Alexander A. Crofts, Frederic M. Poly, Cheryl P. Ewing, Janelle M. Kuroiwa, Joanna E. Rimmer, Clayton Harro, David Sack, Kawsar R. Talaat, Chad K. Porter, Ramiro L. Gutierrez, Barbara DeNearing, Jessica Brubaker, Renee M. Laird, Alexander C. Maue, Kayla Jaep, Ashley Alcala, David R. Tribble, Mark. S. Riddle, Amritha Ramakrishnan, Andrea J. McCoy, Bryan W. Davies, Patricia Guerry, M. Stephen Trent
NATURE MICROBIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Matthew Slattery, Lijia Ma, Rebecca F. Spokony, Robert K. Arthur, Pouya Kheradpour, Anshul Kundaje, Nicolas Negre, Alex Crofts, Ryan Ptashkin, Jennifer Zieba, Alexander Ostapenko, Sarah Suchy, Alec Victorsen, Nader Jameel, A. Jason Grundstad, Wenxuan Gao, Jennifer R. Moran, E. Jay Rehm, Robert L. Grossman, Manolis Kellis, Kevin P. White
Article
Microbiology
Yi-Hsing Chen, Yuyen Lin, Aya Yoshinaga, Benazir Chhotani, Jenna L. Lorenzini, Alexander A. Crofts, Shou Mei, Roderick I. Mackie, Yoshizumi Ishino, Isaac K. O. Cann
JOURNAL OF BACTERIOLOGY
(2009)
Article
Multidisciplinary Sciences
Alexander A. Crofts, Simone M. Giovanetti, Erica J. Rubin, Frederic M. Poly, Ramiro L. Gutierrez, Kawsar R. Talaat, Chad K. Porter, Mark S. Riddle, Barbara DeNearing, Jessica Brubaker, Milton Maciel, Ashley N. Alcala, Subhra Chakraborty, Michael G. Prouty, Stephen J. Savarino, Bryan W. Davies, M. Stephen Trent
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Microbiology
Carmen M. Herrera, Alexander A. Crofts, Jeremy C. Henderson, S. Cassandra Pingali, Bryan W. Davies, M. Stephen Trent
Article
Cell Biology
Hyangyee Oh, Matthew Slattery, Lijia Ma, Alex Crofts, Kevin P. White, Richard S. Mann, Kenneth D. Irvine