期刊
JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
卷 13, 期 3, 页码 243-248出版社
KOREAN SOC APPLIED ENTOMOLOGY
DOI: 10.1016/j.aspen.2010.04.003
关键词
Non-destructive; Insect; DNA; Extraction; Arthropod; Rapid; Taxonomy
类别
资金
- Australian Government
Preparation of arthropods for morphological identification often damages or destroys DNA within the specimen. Conversely, DNA extraction methods often destroy the external physical characteristics essential for morphological identification. We have developed a rapid, simple and non-destructive DNA extraction technique for arthropod specimens. This technique was tested on four arthropod orders, using specimens that were fresh, preserved by air drying, stored in ethanol, or collected with sticky or propylene glycol traps. The technique could be completed in 20 min for Coleoptera, Diptera and Hemiptera, and 2 min for the subclass Acarina, without significant distortion, discolouration, or other damage to the specimens. (C) Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society, 2010 Published by Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据