Article
Multidisciplinary Sciences
Joshua L. C. Wong, Sophia David, Julia Sanchez-Garrido, Jia Z. Woo, Wen Wen Low, Fabio Morecchiato, Tommaso Giani, Gian Maria Rossolini, Konstantinos Beis, Stephen J. Brett, Abigail Clements, David M. Aanensen, Silvi Rouskin, Gad Frankel
Summary: This study investigates the impact of mutations in OmpK36 on carbapenem resistance in Klebsiella pneumoniae. The researchers found that a recurrent 25c > t transition in ompK36 increases resistance by reducing the expression of OmpK36 in the outer membrane. This mutation attenuates the bacteria in a mouse pneumonia model but leads to treatment failure in the context of carbapenem therapy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Wenbo Zhao, Shihong Li, Stefan Schwarz, Aijuan Li, Hong Yao, Xiang-Dang Du
Summary: The study revealed the widespread presence of bla(NDM-5) gene in porcine Klebsiella pneumoniae isolates, posing a serious threat to public health. The detection of high-risk bla(NDM-5)-carrying K. pneumoniae ST340 in food-producing animals highlights the urgent need for surveillance and control measures to prevent further dissemination.
VETERINARY MICROBIOLOGY
(2021)
Article
Genetics & Heredity
Cuidan Li, Xiaoyuan Jiang, Tingting Yang, Yingjiao Ju, Zhe Yin, Liya Yue, Guannan Ma, Xuebing Wang, Ying Jing, Xinhua Luo, Shuangshuang Li, Xue Yang, Fei Chen, Dongsheng Zhou
Summary: The dissemination of carbapenemase-producing Klebsiella pneumoniae (cpKP) is driven by specific combinations of host-blaKPC-carrying plasmids, which are associated with strong population expansion in 2007-2008. This study provides insights into the molecular epidemiology and genetic basis for the spread of ST11/CG258 cpKP in China.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2022)
Article
Microbiology
Michelle Kalu, Karen Tan, Marquerita Algorri, Peter Jorth, Annie Wong-Beringer
Summary: Carbapenem-resistant Klebsiella pneumoniae (CRKP) can undergo adaptive changes in carbon metabolism, carbapenem resistance, and biofilm production during chronic colonization and recurrent urinary tract infections. These adaptations enable CRKP to persist in the human body for extended periods despite repeated antibiotic therapy.
Article
Biochemistry & Molecular Biology
Nicola Cuscino, Ayesha Fatima, Vincenzo Di Pilato, Matteo Bulati, Caterina Alfano, Elisa Monaca, Giuseppina Di Mento, Daniele Di Carlo, Francesca Cardinale, Francesco Monaco, Gian Maria Rossolini, Asif M. Khan, Pier Giulio Conaldi, Bruno Douradinha
Summary: Research conducted a reverse vaccinology approach to identify potential multiepitope vaccine candidates against carbapenem-resistant Klebsiella pneumoniae. Bioinformatics tools were used to analyze their antigenicity and other characteristics, and demonstrated their interaction with immune receptors. A recombinant vaccine was produced using a codon optimization cloning strategy, and immunization simulations showed induced immune responses.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Immunology
Luana Soares de Moraes, Gerusa Luciana Gomes Magalhaes, Joao Gabriel Material Soncini, Marsileni Pelisson, Marcia Regina Eches Perugini, Eliana Carolina Vespero
Summary: This study evaluated clinical data from 107 patients with bloodstream infection caused by Klebsiella pneumoniae and conducted phenotypic and molecular analyses on 50.5% (54/107) of samples that exhibited resistance to carbapenems. The bla(NDM) gene was present in 90.4% (49/54) of these samples, bla(KPC) gene was found in one sample, and 7.4% (4/54) of samples showed no carbapenemase gene. The study identified four main clones and found that 11 samples were not genetically related. The median age of the patients was 58 years old, with a majority of males (60.7%). Comparing patients with and without carbapenem resistance, several factors including ICU stay, renal failure, previous antimicrobial use, Charlson's comorbidity index, invasive procedures, and death showed statistically significant differences. Additionally, renal failure, liver failure, and BSI with extensive-drug resistance (XDR) or pan-drug resistance (PDR) were predictors of increased mortality. The study concluded that patients with carbapenem-resistant pneumonia, regardless of additional resistance to polymyxins, experienced higher mortality rates.
MICROBIAL PATHOGENESIS
(2022)
Article
Microbiology
Na Pei, Wanying Sun, Jingxuan He, Yanming Li, Xia Chen, Tianzhu Liang, Karsten Kristiansen, Wenen Liu, Junhua Li
Summary: The study identified potential marker genes and SNPs associated with carbapenem resistance in Klebsiella pneumoniae, highlighting the complex mechanisms involved such as biofilm formation, efflux pump, and DNA repair. Further investigation of CRKP-related factors is necessary to better understand their contributions to carbapenem resistance. These identified biomarkers could provide targets for future drug interventions or treatments.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Fedor Zurabov, Egor Glazunov, Tatiana Kochetova, Viktoria Uskevich, Valentina Popova
Summary: Klebsiella pneumoniae is a dangerous bacterium associated with various infections, especially in medical facilities. The emergence of drug-resistant and highly virulent strains, as well as the ability to form biofilms, complicates treatment and renders antibiotics ineffective. This study found that using a specific cocktail of bacteriophages can effectively disrupt Klebsiella pneumoniae biofilms.
SCIENTIFIC REPORTS
(2023)
Article
Infectious Diseases
Inglid Fontoura, Thais S. Veriato, Leandro J. Raniero, Maiara L. Castilho
Summary: This study analyzes the synergistic effect of the carbapenem antibiotic Imipenem with silver nanoparticles (AgNPs) against different susceptibility clinical profiles of Klebsiella pneumoniae (K. pneumoniae). The combination of AgNPs and Imipenem showed a reduction in Imipenem concentration in all strains studied, and the synergism was demonstrated through the fractional inhibitory concentration index. The proposed association resulted in inhibitory effects on different K. pneumoniae profiles in both planktonic forms and biofilm, with a peculiar behavior in the Imipenem-resistant profile.
Article
Microbiology
Da-Wei Wei, Nai-Kei Wong, Yuqin Song, Gang Zhang, Chao Wang, Juan Li, Jie Feng
Summary: This study reveals that insertion sequence IS26 mediates rapid amplification of multiple resistance determinants, including bla(KPC-2) and a multiple drug resistance (MDR) region, under sublethal doses of antibiotics. The amplification is accompanied by intricate genetic rearrangement, providing insights into how dynamic gene amplification processes can transform resistance status and complicate diagnosis.
Article
Microbiology
Da-Wei Wei, Nai-Kei Wong, Yuqin Song, Gang Zhang, Chao Wang, Juan Li, Jie Feng
Summary: Our study demonstrates that IS26-mediated amplification of bla(KPC-2) rapidly leads to high-level carbapenem resistance and involves the amplification of a multiple drug resistance region, highlighting how dynamic gene amplification processes occur under antibiotic pressure.
Article
Engineering, Biomedical
Hamideh Karbalaeiheidar, Fatemeh Ashrafi
Summary: In the face of increasing antibiotic-resistant bacteria, it is crucial to find alternatives to conventional antibiotics to combat the serious threat they pose to humanity and the financial burden they impose on communities. This study developed a co-encapsulated formulation of Vancomycin and Gingerol in niosomes and evaluated its effectiveness as an antibacterial agent against carbapenem-resistant Klebsiella pneumoniae strains. The optimized formulation showed sustained drug release, great stability, and enhanced antibacterial and antibiofilm activity against CRKP strains, making it a promising candidate for targeted drug delivery.
BIOMEDICAL MATERIALS
(2023)
Article
Microbiology
Giuseppina Di Mento, Floriana Gona, Giovanna Russelli, Nicola Cuscino, Floriana Barbera, Anna Paola Carreca, Daniele Di Carlo, Francesca Cardinale, Francesco Monaco, Maria Campanella, Alessandra Mularoni, Paolo Grossi, Pier Giulio Conaldi, Bruno Douradinha
Summary: In this study, the epidemiological scenario and molecular characteristics of carbapenem-resistant Klebsiella pneumoniae strains (CR-Kp) at a transplantation hospital in Italy were assessed. The study revealed rapid dissemination of resistance genes among different and new clones of Klebsiella pneumoniae, leading to the emergence of more aggressive and pathogenic multidrug-resistant strains.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Microbiology
Jose Eduardo Toledano-Tableros, Catalina Gayosso-Vazquez, Ma Dolores Jarillo-Quijada, Jose Luis Fernandez-Vazquez, Rayo Morfin-Otero, Eduardo Rodriguez-Noriega, Silvia Giono-Cerezo, Gabriel Gutkind, Jose Di Conza, Jose Ignacio Santos-Preciado, Maria Dolores Alcantar-Curiel
Summary: This study investigated NDM-1-producing K. pneumoniae strains causing bacteremia in a tertiary referral hospital in Mexico. The isolates showed various levels of resistance to different antibiotics, and bla(NDM-1) was found to be the most prevalent carbapenemase gene. The horizontal transfer of bla(NDM-1) gene through IncF-like plasmids among different clones underscores the urgent need for measures to control the spread of NDM-1 carbapenemase in the hospital.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Emilia Galdiero, Maria Michela Salvatore, Angela Maione, Elisabetta de Alteriis, Anna Andolfi, Francesco Salvatore, Marco Guida
Summary: Metabolomic analysis of Candida albicans and Klebsiella pneumoniae dual-species biofilms revealed significant changes in the abundance of approximately 40 compounds, mainly involving central carbon, amino acid, vitamin, and secondary metabolisms. These results were associated with the strict interactions between the two species and the different microbial composition in the early and mature biofilms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Tricia A. Van Laar, Camaron Hole, S. L. Rajasekhar Karna, Christine L. Miller, Robert Reddick, Floyd L. Wormley, J. Seshu
MICROBES AND INFECTION
(2016)
Article
Microbiology
Christine L. Miller, Tricia A. Van Laar, Tsute Chen, S. L. Rajasekhar Karna, Ping Chen, Tao You, Kai P. Leung
Article
Microbiology
Tricia A. Van Laar, Saika Esani, Tyler J. Birges, Bethany Hazen, Jason M. Thomas, Mamta Rawat
Article
Entomology
Cameron J. Osborne, Paul R. Crosbie, Tricia A. Van Laar
JOURNAL OF MEDICAL ENTOMOLOGY
(2019)
Article
Entomology
Cameron J. Osborne, Alya J. Wakeman-Hill, Steven E. Loa, Paul R. Crosbie, Tricia A. Van Laar
JOURNAL OF MEDICAL ENTOMOLOGY
(2020)
Article
Microbiology
Tricia A. Van Laar, Lindsey Biehler, Joel W. G. Slade
Summary: In a recent outbreak of salmonellosis in wild birds, 29 individuals in 12 states were infected, leading to 14 hospitalizations. We report here the draft genome sequence of a multidrug-resistant strain of Salmonella enterica serovar Typhimurium isolated from a bird showing symptoms of salmonellosis.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Ecology
Danielle J. Whittaker, Amruth Atyam, Nathan A. Burroughs, Jonathan M. Greenberg, Travis J. Hagey, Milos V. Novotny, Helena A. Soini, Kevin R. Theis, Tricia A. Van Laar, Joel W. G. Slade
Summary: Avian preen oil is an important source of volatile compounds that convey information about the sender's identity and quality. Social behavior and microbial symbiosis influence the composition of the oil. This study found that sex and subspecies have a stronger influence on the microbial composition than short-term changes in social environment.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2023)
Article
Microbiology
Adam T. Avila, Tricia A. Van Laar, John V. H. Constable, Katherine Waselkov
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2020)
Article
Microbiology
Saika Esani, Tsute Chen, Kai P. Leung, Tricia A. Van Laar
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2019)
Article
Immunology
Ying-Han Lin, Jesus A. Romo, Trever C. Smith, Ann N. Reyes, S. L. Rajasekhar Karna, Christine L. Miller, Tricia A. Van Laar, Raghunandan Yendapally, James P. Chambers, J. Seshu
INFECTION AND IMMUNITY
(2017)
Article
Microbiology
Saika Esani, John V. H. Constable, Tricia A. Van Laar
GENOME ANNOUNCEMENTS
(2017)
Article
Microbiology
Victoria Kennedy, Tricia A. Van Laar, Omoshola Aleru, Michael Thomas, Michelle Ganci, Mamta Rawat
GENOME ANNOUNCEMENTS
(2016)
Article
Microbiology
R. Lee Nelson, Michael A. Castro, Madhusudan Katti, Jonathan A. Eisen, Tricia A. Van Laar
GENOME ANNOUNCEMENTS
(2016)
Article
Microbiology
Tricia A. Van Laar, Tsute Chen, Brandon M. Childers, Ping Chen, Johnathan J. Abercrombie, Kai P. Leung
GENOME ANNOUNCEMENTS
(2014)