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Biochemistry & Molecular Biology
Katerina Stepankova, Dana Marekova, Kristyna Kubasova, Radek Sedlacek, Karolina Turnovcova, Irena Vackova, Sarka Kubinova, Pavol Makovicky, Michaela Petrovicova, Jessica C. F. Kwok, Pavla Jendelova, Lucia Machova Urdzikova
Summary: This study evaluated the physiological changes and potential side effects of 4-methylumbelliferone (4MU) treatment in healthy rats. The results showed increased bile acids, blood sugars, and proteins after 4MU administration, as well as increased interleukins IL10, IL12p70, and IFN gamma. However, these effects were reversed after a 9-week wash-out period.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Azza R. Elkholy, Ahmed R. El-Sheakh, Ghada M. Suddek
Summary: The study demonstrated that nilotinib has a protective role in mitigating hepatic and pulmonary damage induced by paraquat, by reducing tissue damage, improving liver function, decreasing oxidative stress and inflammation, suppressing apoptosis, and modulating the Nrf2/Nf-kB axis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Neurosciences
Ranjana Bhandari, Garima Khanna, Dhriti Kaushik, Anurag Kuhad
Summary: The severity of COVID-19 infection is increasing, with both respiratory and involvement in other organ systems documented. NF-kappa B and Nrf2 pathways play important roles in immune injury, neuro-invasion, and neuroinflammation, potentially serving as therapeutic targets.
MOLECULAR NEUROBIOLOGY
(2021)
Article
Anatomy & Morphology
Lin Tian, Shuang Tao, Chunzhi He, Shanwu Dong, Yongli Chen, Ling Chen, Shu Jiang
Summary: The aim of this study was to investigate the potential impact of Astragaloside IV (AS-IV) on neonatal rat models with necrotizing enterocolitis (NEC) and to confirm the potential involvement of TNF-like ligand 1 A (TL1A) and the NF-KB signaling pathway. NEC neonatal rat models were induced through formula feeding, cold/asphyxia stress, and Lipo-polysaccharide (LPS) administration. The appearance, behavior, skin condition, and pathological status were evaluated in the NEC-modelled rats. Intestinal tissues were examined using H&E staining. The expression of oxidative stress biomarkers (SOD, MDA, GSH-Px) and inflammatory cytokines (TNF-α, IL-13, IL-6) were measured using ELISA and qRT-PCR. TL1A and NF-KB signaling pathway-related proteins were detected using Western blotting and immunohistochemistry. Cell apoptosis was assessed using TUNEL. The results showed that successful NEC neonatal rat models were established, with high expression of TL1A and activation of the NF-KB signaling pathway. AS-IV treatment suppressed TL1A and the NF-KB signaling pathway in NEC rats. Moreover, AS-IV attenuated the inflammatory response in the intestinal tissues of NEC rats by inhibiting TL1A and the NF-Kb signaling pathway.
Article
Biochemistry & Molecular Biology
Ganka Y. Bekyarova, Deyana G. Vankova, Valentina H. Madjova, Nicolai A. Bekyarov, Ayshe S. Salim, Diana G. Ivanova, Stefka M. Stoeva, Daniela I. Gerova, Yoana D. Kiselova-Kaneva
Summary: This study investigated the importance of transcription factors Nrf2 and NF-kB in metabolic syndrome, finding their association with endothelial dysfunction. The results demonstrated significantly elevated levels of Nrf2 and NF-kB expression in patients with MetS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Yanqiu Zhou, Siyuan Wang, Hanlin Luo, Feibo Xu, Jingjing Liang, Chenxu Ma, Luyu Ren, Hui Wang, Yun Hou
Summary: Studies have shown that aflatoxin B1 (AFB1) can induce cytotoxicity in various cells and organs through oxidative stress. However, the toxic effects and mechanism of AFB1 on microglia cells in the spinal cords have not been studied yet. This study found that AFB1 significantly reduced the number of microglia cells and altered the levels of oxidants and antioxidants in the spinal cords of mice. Furthermore, AFB1 induced oxidative stress, promoted apoptosis and cell cycle arrest, and activated NF-KB phosphorylation in BV2 microglia cells. The addition of an active oxygen scavenger effectively reduced these effects. These findings suggest that AFB1 induces microglia cell apoptosis through oxidative stress via the NF-KB signaling pathway.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Roberta Fusco, Rosalba Siracusa, Enrico Gugliandolo, Alessio Filippo Peritore, Ramona D'Amico, Marika Cordaro, Rosalia Crupi, Daniela Impellizzeri, Chiara Gomiero, Salvatore Cuzzocrea, Rosanna Di Paola
Summary: The study shows that PEA/RUT administration can effectively reduce inflammatory cell infiltration, oxidative stress, and cytokine expression during vascular injury, indicating its potential in mitigating vascular damage.
CURRENT MEDICINAL CHEMISTRY
(2021)
Article
Geriatrics & Gerontology
H-C Hsu, Y-L Ke, Y-H Lai, W-C Hsieh, C-H Lin, S-S Huang, J-Y Peng, C-H Chen
Summary: In this study, it was demonstrated that chondroitin sulfate (CS) can induce the proliferation and migration of chondrocytes by activating beta-catenin and enhancing reactive oxygen species (ROS) production. Additionally, CS was found to increase chondrocyte activity and aid in restoring cartilage function in patients with osteoarthritis.
JOURNAL OF NUTRITION HEALTH & AGING
(2022)
Article
Biochemistry & Molecular Biology
Menghui Xu, Ruhao Zhuo, Shengxiang Tao, Yaxu Liang, Chunru Liu, Qingyang Liu, Tian Wang, Xiang Zhong
Summary: This study found that lipopolysaccharide activates the NOD1/NF-κ B pathway through altering m(6)A RNA methylation, promoting the production of inflammatory cytokines and leading to liver inflammation and damage.
Article
Biochemistry & Molecular Biology
Mayada R. Farag, Mahmoud Alagawany, Eman A. A. Mahdy, Enas El-Hady, Shimaa M. Abou-Zeid, Suzan A. Mawed, Mahmoud M. Azzam, Giuseppe Crescenzo, Azza M. A. Abo-Elmaaty
Summary: The study reveals the beneficial effects of Chlorella vulgaris in countering hepato-renal toxicity induced by cadmium. Chlorella vulgaris regulates protein translation, maintains cellular redox homeostasis, and inhibits oxidative damage and inflammation caused by cadmium.
Article
Cell Biology
Kazuki Hasegawa, Ryo Saga, Kentaro Ohuchi, Yoshikazu Kuwahara, Kazuo Tomita, Kazuhiko Okumura, Tomoaki Sato, Manabu Fukumoto, Eichi Tsuruga, Yoichiro Hosokawa
Summary: 4-MU enhances radiosensitization of radioresistant cells by increasing intracellular oxidative stress and suppressing cancer stem cell-like phenotype. These results may be associated with HAS3 or intracellular HA synthesized by HAS3.
Article
Biochemistry & Molecular Biology
Heba R. Ghaiad, Shimaa O. Ali, Asmaa K. Al-Mokaddem, Maha Abdelmonem
Summary: This study demonstrates that sulbutiamine is a drug that can improve diabetic nephropathy by inhibiting multiple signaling pathways and providing renal protection.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Multidisciplinary Sciences
Guoyong Hu, Minjie Zhou, Lina Xu, Donglian Wang
Summary: This study investigates the molecular mechanisms of KLF7 in apoptosis, inflammation, and oxidative stress in H9c2 cells. The results suggest that down-regulation of KLF7 inhibits LPS-induced injury and apoptosis in H9c2 cells, as well as oxidative stress and inflammation. These findings indicate the potential use of KLF7 knockdown as a therapeutic approach for sepsis treatment.
Article
Food Science & Technology
Reem S. Alruhaimi, Emad H. M. Hassanein, May N. Bin-Jumah, Ayman M. Mahmoud
Summary: This study evaluated the protective effects of agomelatine (AGM) against cadmium (Cd)-induced cardiotoxicity in rats and found that AGM modulated the TLR-4/NF-kB pathway and HSP70. The results showed that AGM alleviated Cd-induced myocardial injury, reduced oxidative stress, and enhanced antioxidant effects.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shujian Wu, Xiyu Liao, Zhenjun Zhu, Rui Huang, Mengfei Chen, Aohuan Huang, Jumei Zhang, Qingping Wu, Juan Wang, Yu Ding
Summary: Oxidative stress (OS) is caused by an imbalance between reactive oxygen species and antioxidant levels, leading to inflammation and various diseases. Nrf2 and NF-KB play crucial roles in combating OS and regulating inflammatory responses. Recent studies show that the Nrf2 signaling pathway interacts with the NF-KB signaling pathway in OS, and natural compounds can activate these defense systems to alleviate OS and inflammation.
Review
Biochemistry & Molecular Biology
Angela Avenoso, Giuseppe Bruschetta, Angela D'Ascola, Michele Scuruchi, Giuseppe Mandraffino, Rosa Gullace, Antonino Saitta, Salvatore Campo, Giuseppe M. Campo
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2019)
Article
Biochemistry & Molecular Biology
Manuela Mania, Giuseppe Bruschetta, Angela Avenoso, Angela D'Ascola, Michele Scuruchi, Adele Campo, Giuseppe Acri, Salvatore Campo
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2019)
Review
Chemistry, Medicinal
Angela Avenoso, Giuseppe Bruschetta, Angela D'Ascola, Michele Scuruchi, Giuseppe Mandraffino, Antonino Saitta, Salvatore Campo, Giuseppe M. Campo
MINI-REVIEWS IN MEDICINAL CHEMISTRY
(2020)
Article
Oncology
Natasha Irrera, Angela D'Ascola, Giovanni Pallio, Alessandra Bitto, Federica Mannino, Vincenzo Arcoraci, Michelangelo Rottura, Antonio Ieni, Letteria Minutoli, Daniela Metro, Mario Vaccaro, Domenica Altavilla, Francesco Squadrito
Article
Biotechnology & Applied Microbiology
Alessandro Allegra, Manuela Mania, Angela D'Ascola, Giacomo Oteri, Enrico Nastro Siniscalchi, Angela Avenoso, Vanessa Innao, Michele Scuruchi, Andrea Gaetano Allegra, Caterina Musolino, Salvatore Campo
BIOMED RESEARCH INTERNATIONAL
(2020)
Review
Genetics & Heredity
Fabrizio Bella, Salvatore Campo
Summary: nc-RNAs, specifically lncRNAs, play a crucial role in gene regulation by exerting enhancer or silencer modulation on gene expression. They are highly tissue-specific and can accumulate in the nucleus, cytoplasm, and exosomes. Disruption of these regulatory features may be associated with various pathological conditions, including bipolar disorders.
Article
Biochemistry & Molecular Biology
Giovanni Pallio, Angela D'Ascola, Luigi Cardia, Federica Mannino, Alessandra Bitto, Letteria Minutoli, Giacomo Picciolo, Violetta Squadrito, Natasha Irrera, Francesco Squadrito, Domenica Altavilla
Summary: Metaxalone may have antioxidant and anti-inflammatory effects, with potential for treating neurological disorders associated with neuroinflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Michele Scuruchi, Angela D'Ascola, Angela Avenoso, Annie Zappone, Giuseppe Mandraffino, Salvatore M. Campo, Giuseppe M. Campo
Summary: This study investigated the expression of miR9 and its relationship with cytokines, proteolytic enzymes, and apoptosis in an experimental model of 6mer HA-induced inflammation in human chondrocytes. The researchers found that 6mer HA stimulation significantly increased the expression of TLR-4, CD44, IL-8, IL-18, MMP-9, ADAMTS-5, BAX, and BCL-2 mRNAs, as well as related proteins and NF-kB activation. Upregulation of miR9 was also observed in response to 6mer HA. Transfection of chondrocytes with miR-9 mimic decreased the expression of inflammatory cytokines, metalloproteases, and pro-apoptotic molecules, while transfection with miR9 inhibitor increased their expression. However, the activities of TLR-4 and CD44 were not affected by miR9 mimic/inhibitor, but NF-kB activation was significantly affected.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Biochemistry & Molecular Biology
Federica Aliquo, Aurelio Minuti, Angela Avenoso, Giuseppe Mandraffino, Giuseppe Maurizio Campo, Salvatore Campo, Angela D'Ascola, Michele Scuruchi
Summary: This study investigated the role of endocan in non-small-cell lung carcinoma using an in vitro model and found that knockdown of endocan can inhibit cell migration and proliferation, as well as reduce the activation of related signaling pathways. These findings suggest that endocan promotes a more aggressive cancer cell phenotype in NSCLC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Michele Scuruchi, Federica Aliquo, Angela Avenoso, Giuseppe Mandraffino, Giovanna Vermiglio, Aurelio Minuti, Salvatore Campo, Giuseppe Maurizio Campo, Angela D'Ascola
Summary: Increased endocan expression was found in arthritic patients and chondrocytes stimulated with IL-1ss. The knockdown of endocan significantly reduced the expression of pro-angiogenic molecules and NF-kB activation in IL-1ss-induced inflammation, suggesting a role for endocan in the mechanisms of cell migration, invasion, and angiogenesis in arthritic joints.
Article
Genetics & Heredity
Fabrizio Bella, Maria Rosaria Anna Muscatello, Angela D'Ascola, Salvatore Campo
Summary: This study aims to evaluate the gene expression levels of non-coding RNAs highly expressed in the central nervous system in drug-naive/drug-free bipolar and panic patients. The results show that the expression levels of miR-221, MALAT1, and GAS5 are significantly up-regulated in bipolar patients after therapy, while all non-coding RNAs investigated are down-regulated in panic disorder patients after therapy.
Article
Biochemistry & Molecular Biology
Angela D'Ascola, Michele Scuruchi, Rosaria Maddalena Ruggeri, Angela Avenoso, Giuseppe Mandraffino, Teresa Manuela Vicchio, Salvatore Campo, Giuseppe Maurizio Campo
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2020)