Article
Microbiology
Philip E. Alabi, Cecile Gautier, Thomas P. Murphy, Xilin Gu, Mathieu Lepas, Vishukumar Aimanianda, Jason K. Sello, Iuliana V. Ene
Summary: Fungi cause billions of infections each year and have a devastating impact on human health. The limited antifungal arsenal and emerging drug-resistant species necessitate the development of new therapies. A screening effort led to the discovery of small molecules that restore the susceptibility of Candida species to antifungals.
Article
Microbiology
Michael A. Pfaller, Cecilia G. Carvalhaes, Lalitagauri M. Deshpande, Paul R. Rhomberg, Mariana Castanheira
Summary: In vitro activity evaluation of four azoles against 660 Aspergillus fumigatus isolates collected from 2017 to 2020 revealed low levels of resistance. Whole genome sequencing identified alterations in CYP51 sequences, with the highest rates of resistance observed for itraconazole and isavuconazole. Multiple alterations were detected in CYP51A, and alterations in CYP51B were also noted.
Article
Microbiology
Cecilia G. G. Carvalhaes, Paul R. R. Rhomberg, Michael D. D. Huband, Michael A. A. Pfaller, Mariana Castanheira
Summary: Isavuconazole is the only FDA-approved antifungal for treating invasive mucormycosis. A global collection of Mucorales isolates was evaluated, and it was found that Isavuconazole displayed good inhibitory activity against most isolates, although there was variation among different species.
Article
Microbiology
Teera Leepattarakit, Orawan Tulyaprawat, Popchai Ngamskulrungroj
Summary: In recent years, there has been a shift in epidemiology from Candida infections to non-albicans species and azole resistance. Factors associated with azole-resistant C. tropicalis isolates include male sex, leukemia, and recent azole administration. 83% of resistant isolates had ERG11 mutations, with A395T being the most common mutation.
Article
Microbiology
Michael A. Pfaller, Shawn A. Messer, Lalitagauri M. Deshpande, Paul R. Rhomberg, Eric A. Utt, Mariana Castanheira
Summary: A total of 36 Candida auris isolates were evaluated for antifungal susceptibility and synergy against anidulafungin plus voriconazole or isavuconazole. The isolates showed resistance to multiple antifungal classes, with varying degrees of synergy observed with different combinations of antifungal drugs. Genetic analysis revealed isolates from different regions were genetically related but distinct. Erg11 alterations were associated with resistance to some antifungal agents.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Seung A. Byun, Yong Jun Kwon, Ga Yeong Lee, Min Ji Choi, Seok Hoon Jeong, Dokyun Kim, Min Hyuk Choi, Seung-Jung Kee, Soo Hyun Kim, Myung Geun Shin, Eun Jeong Won, Jong Hee Shin
Summary: This study analyzed the virulence traits and azole resistance mechanisms of 104 Candida auris isolates collected from Korean hospitals from 1996 to 2022. The results showed a high fluconazole resistance rate among C. auris isolates in Korean hospitals, with two clades identified and clade II isolates exhibiting higher resistance. The isolates from different clades were sensitive to other drugs, and clade II isolates displayed reduced thermotolerance, competitive growth, and virulence.
Article
Microbiology
Eva Conkova, Martina Proskovcova, Peter Vaczi, Zuzana Malinovska
Summary: This study aimed to investigate the intensity of biofilm production by Malassezia pachydermatis strains isolated from the ear canal of healthy dogs and determine their susceptibility to three commonly used azole antifungal drugs. The results showed that higher concentrations of antifungal agents are needed to treat infections caused by biofilm-forming strains of M. pachydermatis.
Article
Infectious Diseases
Alicia Gomez-Lopez, Susana Sanchez Galiano, Sheila Ortega Madueno, Cristina Carballo Gonzalez
Summary: The study aimed to assess patient exposure to isavuconazole (ISZ) through therapeutic antifungal monitoring. A simple and efficient method was developed and validated to monitor ISZ blood concentrations, and it was found that ISZ achieved excellent blood concentrations compared to other azole drugs. The concentrations varied depending on the patient's age.
Article
Infectious Diseases
Simone Carradori, Alessandra Ammazzalorso, Barbara De Filippis, Ahmet Fatih Sahin, Atilla Akdemir, Anastasia Orekhova, Graziana Bonincontro, Giovanna Simonetti
Summary: Fungal pathogens cause over a billion human infections annually. In this study, a library of imidazole- and triazole-based compounds were screened and an imidazole carbamate 12 was identified as a potent antifungal against Candida glabrata and Candida krusei biofilms. Furthermore, compound 12 showed a significant reduction in Candida infection in Galleria mellonella larvae without any toxicity. In silico predictions indicated that the combination of the imidazole core, the carbamate, and the p-chlorophenyl group are important for strong interactions with lanosterol 14 alpha-demethylase and fungal carbonic anhydrase, with the S-enantiomer being more productive in these interactions.
Article
Infectious Diseases
Haisheng Chen, Hui Li, Cunxian Duan, Chuanjie Song, Zuoliang Peng, Wenna Shi
Summary: This study demonstrated that the combination of oridonin (ORI) with azole drugs showed potent synergism and increased sensitivity in azole-resistant Candida albicans. By inhibiting drug efflux and promoting apoptosis, ORI provided new therapeutic strategies to overcome azole resistance in Candida albicans.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Mycology
Siyue Kan, Nana Song, Qiuyu Pang, Huan Mei, Hailin Zheng, Dongmei Li, Fan Cui, Guixia Lv, Ruifang An, Ping Li, Zhengai Xiong, Shangrong Fan, Mengzhen Zhang, Yanhong Chen, Qiao Qiao, Xudong Liang, Manhua Cui, Dongyan Li, Qinping Liao, Xiaofang Li, Weida Liu
Summary: Vulvovaginal candidiasis is a global public health issue, and the optimal treatment for recurrent VVC has not been established. In vitro antifungal susceptibility profiling of VVC isolates from different regions of China can provide significant improvements for the control and treatment of this disease.
Article
Microbiology
Erick Martinez-Herrera, Maria Guadalupe Frias-De-Leon, Rigoberto Hernandez-Castro, Eduardo Garcia-Salazar, Roberto Arenas, Esther Ocharan-Hernandez, Carmen Rodriguez-Cerdeira
Summary: There is variability in the rates of resistance to antifungal agents in Candida glabrata in different regions worldwide. Limited data are available on the antifungal resistance in C. glabrata in Ibero-America. A systematic review was conducted to determine the antifungal resistance in C. glabrata in this region over the last five years. The use of azoles and echinocandins varied among different countries. The limited information emphasizes the need for species identification and antifungal susceptibility tests to prevent the development of antifungal resistance.
Article
Immunology
Patrick Schwarz, Ilya Nikolskiy, Anne-Laure Bidaud, Frank Sommer, Gert Bange, Eric Dannaoui
Summary: In this study, the interactions between isavuconazole and colistin were evaluated against common Candida strains. The results showed that there were synergistic or indifferent interactions between the two drugs, but no antagonistic interaction was observed.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Petra V. Schwarz, Ilya Nikolskiy, Eric Dannaoui, Frank Sommer, Gert Bange, Patrick Schwarz
Summary: In vitro interactions between broad-spectrum azole isavuconazole and flavonoid isoquercitrin were evaluated against Candida strains, showing synergistic effect in C. glabrata strains but no effect in other species.
Article
Chemistry, Medicinal
Vinicius Augusto Campos Peret, Rubia Castro Fernandes Melo Reis, Saulo Fehelberg Pinto Braga, Monique Dias Benedetti, Ivo Santana Caldas, Diogo Teixeira Carvalho, Luiz Felipe de Andrade Santana, Susana Johann, Thiago Belarmino de Souza
Summary: This article describes the design, synthesis, and antifungal activity of new imidazoles and 1,2,4-triazoles derived from eugenol and dihydroeugenol. These new compounds showed significant antifungal activity against Candida sp. and Cryptococcus gattii, with some being more active than reference drugs. The most promising compounds, eugenol-imidazole 13 and dihydroeugenol-imidazole 14, exhibited strong antifungal activity against Candida albicans and multi-resistant Candida auris, suggesting their potential as new clinically antifungal agents.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Marija Ivanov, Abhilash Kannan, Dejan S. Stojkovic, Jasmina Glamoclija, Ricardo C. Calhelha, Isabel C. F. R. Ferreira, Dominique Sanglard, Marina Sokovic
Summary: The study investigated the antifungal activity of camphor and eucalyptol on Candida species, revealing that camphor showed excellent antifungal properties with the potential to reduce fungal virulence traits, while eucalyptol exhibited cytotoxic effects and was deemed unsuitable as an antifungal candidate. Both compounds had different effects on efflux pump gene expression, with camphor downregulating CDR1 and eucalyptol upregulating CDR2.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Medicinal
Marija Ivanov, Abhilash Kannan, Dejan S. Stojkovic, Jasmina Glamoclija, Ricardo C. Calhelha, Isabel C. F. R. Ferreira, Dominique Sanglard, Marina Sokovic
Summary: This study focused on flavonoids, including luteolin, quercitrin, etc., and their impact on fungal infections and antifungal mechanisms, finding that some substances such as isoquercitrin have the ability to inhibit fungal growth and affect the expression of genes related to fungal drug resistance.
Article
Microbiology
Jizhou Li, Alix T. Coste, Maroussia Liechti, Daniel Bachmann, Dominique Sanglard, Frederic Lamoth
Summary: Candida auris is a novel Candida species with global spread and multidrug resistance. This study identified two previously unreported mutations in ERG11 and TAC1b genes, which were found to be associated with fluconazole resistance. Further research is needed to better understand the mechanisms underlying these mutations in azole resistance.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Marion Aruanno, Samantha Gozel, Isabelle Mouyna, Josie E. Parker, Daniel Bachmann, Patricia Flamant, Alix T. Coste, Dominique Sanglard, Frederic Lamoth
Summary: A pan-azole-resistant strain with overexpression of drug transporters was generated in vitro. The transporter inhibitor milbemycin oxime was found to inhibit drug transporters and enhance azole activity. This study provides insight into mechanisms of azole stress adaptation and proposes drug transporter inhibition as a potential therapeutic target.
Article
Microbiology
Dominique Sanglard
Summary: In Candida albicans, calcium ions play a crucial role in regulating signaling pathways, cell polarization, and hyphal extension. Aequorin-based Ca2+ sensing system provides a valuable tool for studying Ca2+ homeostasis in this fungal pathogen. Mutants in key Ca2+ channels showed reduced response to alkaline stress, highlighting the importance of these channels in Ca2+ uptake and homeostasis.
Article
Virology
Damien Jacot, Milo Moraz, Alix T. Coste, Christele Aubry, Jilian A. Sacks, Gilbert Greub, Antony Croxatto
Summary: This study evaluated the performance of 16 serological tests for SARS-CoV-2, finding 13 tests with over 90% sensitivity at 15 days post-symptom onset and 97% specificity. No systematic cross-reactivity was observed, but heterogeneities across tests were found.
JOURNAL OF CLINICAL VIROLOGY
(2021)
Article
Microbiology
Jizhou Li, Alix T. Coste, Daniel Bachmann, Dominique Sanglard, Frederic Lamoth
Summary: Candida auris is an emerging yeast pathogen with significant antifungal resistance. This study found that the transcription factor Mrr1 and the drug transporter Mdr1 may contribute to azole resistance in C. auris through a mechanism independent of ERG11 mutations and CDR1 upregulation.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Christina Lemberg, Kontxi Martinez de San Vicente, Ricardo Frois-Martins, Simon Altmeier, Van Du T. Tran, Sarah Mertens, Sara Amorim-Vaz, Laxmi Shanker Rai, Christophe D'Enfert, Marco Pagni, Dominique Sanglard, Salome LeibundGut-Landmann
Summary: The fungus Candida albicans colonizes the oral cavity and maintains a commensal relationship with the host by inhibiting filamentation and virulence gene expression, and adapting to nutrient-poor conditions. Understanding the determinants of commensalism in the oral cavity can provide insights into the prevention and treatment of oral diseases caused by C. albicans.
Article
Immunology
Nicolas Pellaton, Dominique Sanglard, Frederic Lamoth, Alix T. Coste
Summary: By comparing two different commercial microdilution antifungal susceptibility testing (AFST) methods, this study demonstrates the valuable use of both methods in detecting antifungal resistance due to point mutations in antifungal resistance genes. It also highlights the difficulty of conducting conclusive analyses without antifungal gene sequence data as a gold standard.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Editorial Material
Immunology
Daisuke Hagiwara, Sabine Fillinger, Dominique Sanglard
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Sanne Schrevens, Eric Durandau, Van Du T. Tran, Dominique Sanglard
Summary: This study investigated the transcriptomic profile of Candida glabrata during urinary tract infection in mice for the first time using fungal RNA enrichment. The results showed that adhesins, proteases, nitrogen metabolism, and regulation play vital roles in C. glabrata infection. Key genes involved in nitrogen metabolism and the glyoxylate cycle were identified as important for virulence.
Review
Multidisciplinary Sciences
Neil A. R. Gow, Carolyn Johnson, Judith Berman, Alix T. Coste, Christina A. Cuomo, David S. Perlin, Tihana Bicanic, Thomas S. Harrison, Nathan Wiederhold, Mike Bromley, Tom Chiller, Keegan Edgar
Summary: Prior to the SARS-CoV-2 pandemic, antibiotic resistance was considered a major global healthcare priority. Although fungal infections have not received enough public attention, the annual deaths caused by these infections are comparable to or even exceed the global mortalities due to malaria, tuberculosis, or HIV. The rise of antifungal drug resistant strains has worsened the impact of fungal infections on human health. This review aims to illuminate the problem of antifungal resistance and suggest possible measures to mitigate this growing threat.
NATURE COMMUNICATIONS
(2022)
Article
Medicine, General & Internal
Alix T. Coste, Adrian Egli, Jacques Schrenzel, Beatrice Nickel, Andrea Zbinden, Reto Lienhard, Alexis Dumoulin, Martin Risch, Gilbert Greub
Summary: The IVDR regulation poses a major challenge for diagnostic microbiology laboratories due to its complexity and risks. The emergence of new pathogens further complicates the regulation, negatively impacting the development of new diagnostic tests. Additionally, the regulation affects the availability and costs of diagnostic tests, posing a risk to public health.
Article
Biotechnology & Applied Microbiology
S. Schrevens, D. Sanglard
Summary: This study presents a new tetracycline-inducible system for gene expression in Candida glabrata, a fungal pathogen. The system can be used both in vitro and in vivo in any genetic background of C. glabrata, and was successfully employed in a mouse model to demonstrate the importance of a specific gene in the virulence of C. glabrata.
FEMS YEAST RESEARCH
(2022)