期刊
JOURNAL OF APPLIED POLYMER SCIENCE
卷 123, 期 2, 页码 637-645出版社
WILEY-BLACKWELL
DOI: 10.1002/app.34532
关键词
poly(ethylene glycol); poly(2-hydroxyethyl methacrylate); interpenetrating hydrogels; artificial cornea
资金
- National Research Foundation (NRF)
- Ministry of Education, Science and Technology [M10755020001-07N5502-00110, R11-2007-050-03002-0]
- Seoul Research and Business Development Program [10816]
- National Research Foundation of Korea [2007-2004045] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
To enhance the mechanical strength of poly(ethylene glycol)(PEG) gels and to provide functional groups for surface modification, we prepared interpenetrating (IPN) hydrogels by incorporating poly(2-hydroxyethyl methacrylate)(PHEMA) inside PEG hydrogels. Formation of IPN hydrogels was confirmed by measuring the weight percent gain of the hydrogels after incorporation of PHEMA, as well as by ATR/FTIR analysis. Synthesis of IPN hydrogels with a high PHEMA content resulted in optically transparent and extensively crosslinked hydrogels with a lower water content and a 6 similar to 8-fold improvement in mechanical properties than PEG hydrogels. Incorporation of less than 90 wt % PHEMA resulted in opaque hydrogels due to phase separation between water and PHEMA. To overcome the poor cell adhesion properties of the IPN hydrogels, collagen was covalently grafted to the surface of IPN hydrogels via carbamate linkages to hydroxyl groups in PHEMA. Resultant IPN hydrogels were proven to be noncytotoxic and cell adhesion study revealed that collagen immobilization resulted in a significant improvement of cell adhesion and spreading on the IPN hydrogel surfaces. The resultant IPN hydrogels were noncytotoxic, and a cell adhesion study revealed that collagen immobilization improved cell adhesion and spreading on the IPN hydrogel surfaces significantly. These results indicate that PEG/PHEMA IPN hydrogels are highly promising biomaterials that can be used in artificial corneas and a variety of other load-bearing tissue engineering applications. (C) 2011 Wiley Periodicals, Inc. J Appl Polym Sci 123:637-645, 2012
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据