期刊
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
卷 59, 期 9, 页码 5340-5347出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.00589-15
关键词
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资金
- European Union Seventh Framework Programme (EvoTAR) [HEALTH-F3-2011-2011-282004]
- Italian Ministry for Foreign Affairs (Fortalecimiento de la red de salud del Chaco Boliviano: una perspectiva comunitaria)
- Ente Cassa di Risparmio di Firenze (Florence, Italy)
- Regione Toscana (Italy) (Toscana e Chaco, 25 anni di cooperazione sanitaria: un passo decisivo verso il contenimento della diffusione delle resistenze batteriche agli antibiotici)
During the last decade, a significant diffusion of CTX-M-type extended-spectrum beta-lactamases (ESBLs) was observed in commensal Escherichia coli from healthy children in the Bolivian Chaco region, with initial dissemination of CTX-M-2, which was then replaced by CTX-M-15 and CTX-M-65. In this work, we demonstrate that the widespread dissemination of CTX-M-65 observed in this context was related to the polyclonal spreading of an IncI1 sequence type 71 (ST71) epidemic plasmid lineage. The structure of the epidemic plasmid population was characterized by complete sequencing of four representatives and PCR mapping of the remainder (n = 16). Sequence analysis showed identical plasmid backbones (similar to that of the reference IncI1 plasmid, R64) and a multiresistance region (MRR), which underwent local microevolution. The MRR harbored genes responsible for resistance to beta-lactams, aminoglycosides, florfenicol, and fosfomycin (with microevolution mainly consisting of deletion events of resistance modules). The blaCTX-M-65 module harbored by the IncI1 ST71 epidemic plasmid was apparently derived from IncN-type plasmids, likely via IS26-mediated mobilization. The plasmid could be transferred by conjugation to several different enterobacterial species (Escherichia coli, Cronobacter sakazakii, Enterobacter cloacae, Klebsiella oxytoca, Klebsiella pneumoniae, and Salmonella enterica) and was stably maintained without selective pressure in these species, with the exception of K. oxytoca and S. enterica. Fitness assays performed in E. coli recipients demonstrated that the presence of the epidemic plasmid was apparently not associated with a significant biological cost.
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