Article
Materials Science, Multidisciplinary
Hafez Jafari, Houman Alimoradi, Christine Delporte, Katrien V. Bernaerts, Reza Heidari, Daria Podstawczyk, Seyyed Vahid Niknezhad, Amin Shavandi
Summary: In this study, dual network hydrogels with antioxidant and antibacterial activities were designed using marine poly- and oligosaccharides. The hydrogels exhibited favorable physicochemical properties and biodegradability, and showed antioxidant and antibacterial effects, promoting wound healing.
APPLIED MATERIALS TODAY
(2022)
Article
Biochemistry & Molecular Biology
Xin Zheng, Yining Chen, Nianhua Dan, Weihua Dan, Zhengjun Li
Summary: Epoxidized N-(2-hydroxypropyl)-3-trimethylammonium chitosan chloride (EHTCC) was synthesized by adding epoxy group to HTCC, and used for modifying porcine acellular dermal matrix (pADM), resulting in significantly improved thermal stability and mechanical properties of EHTCC-pADM while maintaining the natural conformation of the matrix. The enhanced hydrophilicity from the modification facilitated cell adhesion and growth, and cytotoxicity experiments showed no adverse effects on biocompatibility. In vivo experiments demonstrated that EHTCC-pADM could promote wound healing effectively.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Materials Science, Multidisciplinary
Tanmoy Ghosh, Deveswaran Rajamanickam, Damodar Nayak, Bharath Srinivasan, B. V. Basavaraj
Summary: This study developed new antimicrobial scaffolds based on crosslinked CMCh/Gel biopolymers and conducted comprehensive investigations on their properties and applications. The results showed that the scaffolds had favorable physical and chemical characteristics, antibacterial properties, cytocompatibility, and potential for promoting wound healing. These scaffolds have high applicative potential in biomedical applications such as skin tissue regeneration and wound healing.
MATERIALS TODAY COMMUNICATIONS
(2023)
Review
Pharmacology & Pharmacy
Anna Yusuf Aliyu, Oluwatoyin A. Adeleke
Summary: Chronic wounds in poorly managed diabetics are difficult to heal due to long-term uncontrolled blood glucose levels. To prevent complications, special medical care is needed. Nanofibrous scaffolds are a promising treatment due to their flexibility, ability to load bioactive compounds, and biomimetic environment for cell proliferation.
Review
Biotechnology & Applied Microbiology
Hamed Nosrati, Reza Aramideh Khouy, Ali Nosrati, Mohammad Khodaei, Mehdi Banitalebi-Dehkordi, Korosh Ashrafi-Dehkordi, Samira Sanami, Zohreh Alizadeh
Summary: The skin serves as the body's primary barrier against external pathogens and maintaining homeostasis. Damage to the skin can have significant impacts on health and quality of life. Tissue engineering aims to enhance tissue regeneration, with improving angiogenesis being one of the most effective treatments for skin tissue regeneration.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Polymer Science
Maria Budai-Szucs, Marco Ruggeri, Angela Faccendini, Attila Leber, Silvia Rossi, Gabor Varga, Maria Cristina Bonferoni, Peter Valyi, Katalin Burian, Erzsebet Csanyi, Giuseppina Sandri, Franca Ferrari
Summary: This study designed and developed nanofibrous scaffolds based on biopolymers to restore tissue integrity and treat bacterial infections in periodontitis. The scaffolds were characterized by their chemical and physical properties, as well as preclinical properties, demonstrating effectiveness in supporting cell adhesion and proliferation while inhibiting bacterial growth. The scaffold composed of gelatin and low molecular weight chitosan showed promising results in vitro for both fibroblast and osteoblast adhesion and proliferation as well as inhibiting the growth of specific bacteria involved in periodontitis.
Article
Biochemistry & Molecular Biology
Ziqiang Zhou, Xuejuan Zhang, Lijun Xu, Huangjie Lu, Yuying Chen, Chuanbin Wu, Ping Hu
Summary: In this study, a novel hydrogel was designed as a wound dressing material for diabetic wounds. The hydrogel showed good biocompatibility and enhanced wound healing in diabetic rats.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Engineering, Biomedical
Josh Karam, Breahna J. Singer, Hiromi Miwa, Limin H. Chen, Kajal Maran, Mahdi Hasani, Sarahi Garza, Bianca Onyekwere, Hsin-Chih Yeh, Song Li, Dino Di Carlo, Stephanie K. Seidlits
Summary: This study demonstrates that high molecular weight hyaluronic acid (HMW HA) promotes viability and tube formation in human cerebral microvascular endothelial cells (HCMVECs), both in soluble and crosslinked forms. The HMW HA in crosslinked form also supports vascularization through inducing CD44 receptor clustering. This finding is significant for the design of hyaluronic acid hydrogels with predictable bioactivity.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Applied
Hang Li, Xujie Zhou, Lei Luo, Qiang Ding, Shunqing Tang
Summary: CS-PEG-HA hydrogel exhibits outstanding adhesion and hemostasis properties, enabling rapid repair of liver bleeding and accelerated healing of skin wounds.
CARBOHYDRATE POLYMERS
(2022)
Review
Biotechnology & Applied Microbiology
Jianghui Qin, Fang Chen, Pingli Wu, Guoming Sun
Summary: Wound healing is a complex biological process with bioengineered scaffolds playing a key role in regenerative therapy. Although advancements have been made, challenges remain in achieving ideal treatment for deep cutaneous injuries. Designing innovative scaffolds, precursor development, and therapeutic interventions hold promise for future wound healing strategies.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Dharshan Sivaraj, Kellen Chen, Arhana Chattopadhyay, Dominic Henn, Wanling Wu, Chikage Noishiki, Noah J. Magbual, Smiti Mittal, Alana M. Mermin-Bunnell, Clark A. Bonham, Artem A. Trotsyuk, Janos A. Barrera, Jagannath Padmanabhan, Michael Januszyk, Geoffrey C. Gurtner
Summary: Cutaneous wounds are becoming a growing global health burden due to an aging population and increasing incidence of diseases. Cell therapy combined with hydrogels shows potential in improving treatment efficacy for wound healing.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Rajib Dey, Riya Mukherjee, Jayanta Haldar
Summary: This report introduces an intrinsically antimicrobial hydrogel (HyDex) that exhibits broad-spectrum antimicrobial activity, hemostatic ability, and rapid wound closure efficacy. The hydrogel demonstrates effective antibacterial and antifungal effects, rapid blood clotting, and reduction in inflammatory responses in various models, showing great potential in addressing resistant bacteria, fungal infections, bleeding, and inflammation.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Engineering, Multidisciplinary
Tongtong Shi, Hanzhi Lu, Jianyong Zhu, Xiaojun Zhou, Chuanglong He, Fulun Li, Guang Yang
Summary: A multifunctional hydrogel dressing composed of keratin, protocatechuic aldehyde (PA) and iron ions was successfully fabricated, which exhibited injectability, self-healing ability, tissue adhesion, antibacterial properties and anti-inflammation effects. The incorporation of phellopterin (PP) further enhanced the anti-inflammatory properties of the hydrogel. In experiments with diabetic mice, the PP-loaded hydrogel significantly shortened inflammation duration, promoted macrophage polarization towards anti-inflammatory phenotype, and accelerated wound repair.
COMPOSITES PART B-ENGINEERING
(2023)
Article
Pharmacology & Pharmacy
Pablo Edmundo Antezana, Sofia Municoy, Maria Ines Alvarez-Echazu, Pablo Luis Santo-Orihuela, Paolo Nicolas Catalano, Taleb H. Al-Tel, Firoz Babu Kadumudi, Alireza Dolatshahi-Pirouz, Gorka Orive, Martin Federico Desimone
Summary: This review discusses the latest developments in skin tissue engineering using 3D bioprinting as a new tool. It provides an overview of current bioprinting methods and summarizes bioink formulations, parameters, and properties. Representative examples and advances in the field, as well as limitations and future needs, are also discussed.
Article
Materials Science, Multidisciplinary
Yushi Wang, Xueru Xiong, Caihong Lin, Wen Huang, Yunhua Chen, Lin Wang, Sa Liu, Yong-Guang Jia
Summary: Poly(l-glutamic acid) (PLGA), a polypeptide prepared by bacteria fermentation, has broad applications in tissue engineering. Through the combination of host-guest crosslinking and the photo-crosslinking of a dopamine derivative, a PLGA-based dual-network hydrogel with self-healing and strong adhesion properties is prepared. The hydrogel shows rapid healing and tissue regeneration in vivo.
MACROMOLECULAR MATERIALS AND ENGINEERING
(2022)
Article
Cell & Tissue Engineering
Jeremie D. Oliver, Shihai Jia, Leslie R. Halpern, Emily M. Graham, Emma C. Turner, John S. Colombo, David W. Grainger, Rena N. D'Souza
Summary: Clefts of the lip and/or palate are common orofacial birth defects, with challenges in correcting severe cleft defects through surgery alone. Advances in tissue engineering offer new avenues to stimulate bone formation at the site of surgical correction for palatal clefts, potentially improving treatment outcomes.
TISSUE ENGINEERING PART B-REVIEWS
(2021)
Article
Obstetrics & Gynecology
Goro Kuramoto, Ibrahim A. Hammad, Brett D. Einerson, Amanda A. Allshouse, Michelle Debbink, David W. Grainger, Robert M. Silver, Teruo Okano
Summary: This study demonstrates the feasibility of transplanting human umbilical cord mesenchymal stem cell sheets in a rat model and highlights the potential benefits of reducing uterine fibrosis and scarring. The use of stem cell sheets significantly reduces fibroblast infiltration and uterine scar tissue formation during healing.
AMERICAN JOURNAL OF PERINATOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Kyungsook Kim, Sophia Bou-Ghannam, Sumako Kameishi, Masatoshi Oka, David W. Grainger, Teruo Okano
Summary: The evolution of drug discovery in the early 20th century saw a shift from focusing on symptom control to pursuing radical cures, with cell therapy emerging as a promising new treatment targeting specific tissues and dynamic signaling environments. Cell delivery methods play a crucial role in realizing the therapeutic potential of cell therapy, especially in the context of mesenchymal stem cell (MSC) engraftment and bioactive function. Cell sheet technology is presented as a breakthrough in cell therapy, showcasing therapeutic success in tissue regeneration and discussing its anticipated clinical impacts.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Chemistry, Multidisciplinary
James M. Anderson, David W. Grainger
Summary: Sung Wan Kim's initial efforts as an independent investigator were focused on improving the understanding of early events in blood/material interactions, leading to the development of blood compatible materials. His studies were supported by NIH grants for over 40 years and contributed to the fields of biomaterials and non-thrombogenic biomaterial research.
JOURNAL OF CONTROLLED RELEASE
(2021)
Editorial Material
Chemistry, Multidisciplinary
David W. Grainger, Wim Hennink, Raymond Schiffelers, You Han Bae
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Cell & Tissue Engineering
Hallie Thorp, Kyungsook Kim, Sophia Bou-Ghannam, Makoto Kondo, Travis Maak, David W. Grainger, Teruo Okano
Summary: The study investigated the effects of cell sheet multilayering technique on the fabrication of MSC-derived hyaline-like cartilage 3D layered constructs, demonstrating its potential to increase construct thickness and cellular interactions, while also identifying a thickness threshold that hinders chondrogenesis rate and extent.
REGENERATIVE THERAPY
(2021)
Article
Cell & Tissue Engineering
Makoto Kondo, Sumako Kameishi, Kyungsook Kim, Nicolas F. Metzler, Travis G. Maak, Douglas T. Hutchinson, Angela A. Wang, Miki Maehara, Masato Sato, David W. Grainger, Teruo Okano
Summary: This study demonstrates the preclinical safety and efficacy of scaffold-free, human juvenile cartilage-derived-chondrocyte (JCC) sheets. JCCs show stable and high growth potential in vitro, supporting the possibility of scale-up to mass production for commercialization. The engineered JCC sheets show stable hyaline cartilage formation, indicating a promising strategy for clinical cartilage repair.
NPJ REGENERATIVE MEDICINE
(2021)
Review
Pharmacology & Pharmacy
Ulrich Dirnagl, Georg N. Duda, David W. Grainger, Petra Reinke, Ronenn Roubenoff
Summary: Biomedical research accuracy and relevance are costly problems, with issues in data quality, reporting, methodology, and reproducibility. Preclinical models and clinical data are deficient, leading to inefficient translation from basic research to clinical solutions, resulting in wasted resources and limited new clinical solutions for diseases.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Article
Computer Science, Information Systems
Alan H. Morris, Christopher Horvat, Brian Stagg, David W. Grainger, Michael Lanspa, James Orme, Terry P. Clemmer, Lindell K. Weaver, Frank O. Thomas, Colin K. Grissom, Ellie Hirshberg, Thomas D. East, Carrie Jane Wallace, Michael P. Young, Dean F. Sittig, Mary Suchyta, James E. Pearl, Antinio Pesenti, Michela Bombino, Eduardo Beck, Katherine A. Sward, Charlene Weir, Shobha Phansalkar, Gordon R. Bernard, B. Taylor Thompson, Roy Brower, Jonathon Truwit, Jay Steingrub, R. Duncan Hiten, Douglas F. Willson, Jerry J. Zimmerman, Vinay Nadkarni, Adrienne G. Randolph, Martha A. Q. Curley, Christopher J. L. Newth, Jacques Lacroix, Michael S. D. Agus, Kang Hoe Lee, Bennett P. DeBoisblanc, Frederick Alan Moore, R. Scott Evans, Dean K. Sorenson, Anthony Wong, Michael Boland, Willard H. Dere, Alan Crandall, Julio Facelli, Stanley M. Huff, Peter J. Haug, Ulrike Pielmeier, Stephen E. Rees, Dan S. Karbing, Steen Andreassen, Eddy Fan, Roberta M. Goldring, Kenneth Berger, Beno W. Oppenheimer, E. Wesley Ely, Brian W. Pickering, David A. Schoenfeld, Irena Tocino, Russell S. Gonnering, Peter J. Pronovost, Lucy A. Savitz, Didier Dreyfuss, Arthur S. Slutsky, James D. Crapo, Michael R. Pinsky, Brent James, Donald M. Berwick
Summary: Delivering the best care in various clinical settings is a challenging problem. Evidence-based guidelines currently address only a fraction of the care provided by clinicians, especially in underserved areas. The use of validated clinical decision support systems (eActions) could help overcome cognitive limitations in advanced healthcare environments.
JOURNAL OF THE AMERICAN MEDICAL INFORMATICS ASSOCIATION
(2022)
Article
Cell Biology
Sophia Bou-Ghannam, Kyungsook Kim, Makoto Kondo, David W. Grainger, Teruo Okano
Summary: Cell sheet technology is a scaffold-free 3D cell delivery platform that can sustain cell engraftment, survival, and reparative function. Recent evidence shows that cell sheets stimulate mesenchymal stem cells (MSC) to produce paracrine factors. This study introduces a centrifugation strategy to enhance MSC paracrine factor production by stacking and centrifuging cell sheets, resulting in a 3D MSC-delivery platform with boosted therapeutic factor production capacity.
Article
Cell & Tissue Engineering
Masatoshi Oka, Sumako Kameishi, Yun-Kyoung Cho, Sun U. U. Song, David W. W. Grainger, Teruo Okano
Summary: Chronic kidney disease (CKD) is the irreversible loss of nephron function, leading to toxins build-up, inflammation, and fibrosis. Mesenchymal stem/stromal cell (MSC) transplantation is a promising strategy to treat CKD, but concerns remain about kidney decapsulation. In this study, clinically relevant human clonal BMSC (cBMSC) sheets were transplanted onto intact renal capsule, effectively suppressing tubular epithelial cell injury and renal fibrosis in a rat model of ischemia-reperfusion injury (IRI).
TISSUE ENGINEERING PART C-METHODS
(2023)
Article
Cell Biology
Celia M. Dunn, Sumako Kameishi, Yun-Kyoung Cho, Sun U. Song, David W. Grainger, Teruo Okano
Summary: Mesenchymal stromal cells (MSCs) have diverse immunomodulatory functions and hold promise as a treatment for immune-related diseases. In this study, IFN-γ priming and thermoresponsive cultureware were combined to produce transplantable scaffold-free immunosuppressive cell sheets. The use of human clinical-grade single-cell-derived clonal bone marrow-derived MSCs further enhances the clinical relevance of this approach. The results show that MSC sheets produced with IFN-γ priming upregulate expression of immunosuppressive factors and exhibit increased ability to inhibit T-cell proliferation.
Article
Biophysics
Lara J. Gamble, David Radford, David W. Grainger, David G. Castner
Summary: This study characterized the properties of self-assembled monolayers (SAMs) of perfluoroalkanethiols on gold surfaces using XPS, NEXAFS, and ToF-SIMS techniques. It was found that SAMs with longer carbon chains exhibited higher ordering on gold surfaces, while SAMs with shorter carbon chains showed lower ordering.
Editorial Material
Cell Biology
Georg N. Duda, David W. Grainger, Robert E. Guldberg, Gady Goldsobel, Glenn D. Prestwich, Brenna Rauw, Hans-Dieter Volk
Summary: Translational impact assessment is crucial in determining the potential of biomedical research discoveries to be transformed into practical, beneficial products for human health. However, measuring translational success is challenging as it goes beyond commercial achievements and may vary from case to case. Prioritizing societal impact is essential in a globalized society.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Pranothi Mulinti, Dorina Diekjurgen, Kristen Kurtzeborn, Narayanaganesh Balasubramanian, Shane J. Stafslien, David W. Grainger, Amanda E. Brooks
Summary: In this study, a small basic peptide known for its antimicrobial properties was genetically linked to a mechanically robust silk-like peptide. The fusion protein showed both heparin-binding and antimicrobial capabilities, creating a designer silk-like protein with improved hemocompatibility and antimicrobial properties.
BIOENGINEERING-BASEL
(2022)