Article
Biochemistry & Molecular Biology
Danielle Steffen, Michael J. Mienaltowski, Keith Baar
Summary: The effect of mechanical load on tendinopathic tissue varies depending on the type of load. Dynamic loading increases fibrocartilage markers, while long isometric loads stimulate markers of tendon regeneration.
Article
Biochemistry & Molecular Biology
Sasha Coates-Park, Carolyn Lazaroff, Sadeechya Gurung, Josh Rich, Alexandra Colladay, Maura O'Neill, Georgina S. Butler, Christopher M. Overall, William G. Stetler-Stevenson, David Peeney
Summary: Extracellular proteolysis and turnover play crucial roles in tissue homeostasis. MMPs, as the main matrix-degrading enzymes, are regulated by TIMPs. The balance between MMP activity and TIMP levels is essential for healthy tissue homeostasis and can be disrupted in pathological conditions.
Article
Neurosciences
Griffin A. Greco, Mitchell Rock, Matthew Amontree, Maria Fe Lanfranco, Holly Korthas, Sung Hyeok Hong, R. Scott Turner, G. William Rebeck, Katherine Conant
Summary: The APOE4 allele increases the risk for Alzheimer's disease in a dose-dependent manner and is associated with cognitive decline. A study using mice with targeted gene replacement showed that APOE4 reduces neuronal dendritic complexity and impairs learning. APOE4 also reduces gamma oscillation power, important for learning and memory. The present study found that CCL5, an ECM effector, is increased in CSF samples from APOE4 individuals, and TIMPs, which inhibit ECM-degrading enzymes, are increased in APOE4 CSF and brain lysates. Knockout of CCR5, a receptor for CCL5, enhanced gamma power and improved learning and memory.
NEUROBIOLOGY OF DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Wan-Jing Chen, I-Hsuan Lin, Chien-Wei Lee, Yi-Fan Chen
Summary: Aging leads to decline in skeletal muscle function, with reduced regenerative capacity and changes in extracellular matrix structure. This can result in increased collagen deposition and decreased ECM degradation in aged skeletal muscle.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jin Hyung Kim, Hae Dong Jeong, Min Ji Song, Dong Hun Lee, Jin Ho Chung, Seung-Taek Lee
Summary: SOD3 plays a role in reducing oxidative stress and promoting collagen synthesis in skin aging.
Article
Biochemistry & Molecular Biology
Carla U. Doll, Sabine Niebert, Janina Burk
Summary: Tendon lesions are common in both humans and horses, with the healing process often leading to fibrosis and chronic disease. Local mesenchymal stromal cell injection is a accepted therapeutic strategy for acute lesions in horses, but data on its efficacy in chronic tendon disease are limited. However, MSCs have been shown to have a positive effect on fibrotic tissue. This study aimed to investigate the interaction between MSCs and healthy or chronically diseased tendon matrix in horses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Jared L. Zitnay, Allen H. Lin, Jeffrey A. Weiss
Summary: The study found that mechanical damage to tissue structure during repeated loading is mainly caused by collagen denaturation. Faster loading rates result in more creep strain and denaturation, which may serve as a protective measure during high-rate events but also predispose tissues to injury.
ACTA BIOMATERIALIA
(2021)
Article
Biochemistry & Molecular Biology
Yanmiao Fan, Mads Luchow, Adel Badria, Daniel J. Hutchinson, Michael Malkoch
Summary: In this study, placenta powder (PP) made from decellularized human placenta was incorporated into synthetic poly(ethylene glycol) (PEG)-based hydrogels. The PP-incorporated PEG hydrogels showed tunable storage moduli and reduced swelling ratios compared to pristine hydrogels. The hydrogels demonstrated good biocompatibility and the ability to support cell adhesion and proliferation.
Article
Biochemistry & Molecular Biology
Han Na Park, Min Ji Song, Young Eun Choi, Dong Hun Lee, Jin Ho Chung, Seung-Taek Lee
Summary: LRG1 plays a role in skin repair and fibrosis by stimulating the TGF-β signaling pathway. It increases type I collagen secretion and decreases matrix metalloproteinase-1 secretion in fibroblasts. LRG1 can activate the TGF-β pathway and promote collagen deposition while reducing its degradation, potentially slowing down skin aging.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Sarah E. Brashear, Ross P. Wohlgemuth, Gabriella Gonzalez, Lucas R. Smith
Summary: The amount of fibrotic material in muscle is related to contractile function and passive stiffness, but excessive fibrosis does not directly lead to decreased muscle function. Anti-fibrotic therapies should target collagen architecture, particularly large collagen fibers and their alignment, to enhance muscle function and reduce excessive stiffness.
JOURNAL OF PHYSIOLOGY-LONDON
(2021)
Article
Biochemistry & Molecular Biology
Keuri E. Rodrigues, Aline Azevedo, Pricila R. Goncalves, Maria H. B. Pontes, Gustavo M. Alves, Ruan R. Oliveira, Cristine B. Amarante, Joao P. M. Issa, Raquel F. Gerlach, Alejandro F. Prado
Summary: This study evaluated the effects of doxycycline on aortic remodeling, MMP activity, and ROS levels in ApoE(- forward slash -)/OVX mice. The results showed that doxycycline reduced ROS and MMP-2 activity and expression, leading to a decrease in atherosclerotic lesions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Subhajit Konar, Scott M. Bolam, Brendan Coleman, Nicola Dalbeth, Sue R. McGlashan, Sophia Leung, Jillian Cornish, Dorit Naot, David S. Musson
Summary: Tendinopathy is characterized by pathological changes in tendon matrix composition, architecture, and stiffness, with inflammation also playing an important role. This study found that substrate stiffness affects tendon-derived cells and macrophages. Tendon-derived cells showed minor responses to substrate stiffness, while macrophages exhibited a more inflammatory phenotype on non-physiological stiffness substrates. These subtle variations in matrix stiffness may contribute to the onset and progression of tendinopathy.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ghazi O. Bou Ghanem, Dmitry Koktysh, Robert O. Baratta, Brian J. Del Buono, Eric Schlumpf, Lauren K. Wareham, David J. Calkins
Summary: This study investigates the potential of collagen mimetic peptides (CMPs) in repairing damaged collagen in ocular tissues. The results demonstrate that CMPs can restore the stiffness and structure of collagen in optic nerve head tissues, offering a promising therapeutic avenue for ocular disorders involving collagen remodeling and degradation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Brenda Furtado Costa, Tarcisio Navegante de Queiroz Filho, Adeniele Lopes da Cruz Carneiro, Aline Semblano Carreira Falcao, Maria Sueli da Silva Kataoka, Joao de Jesus Viana Pinheiro, Ana Paula Drummond Rodrigues
Summary: This study examines the presence and activity of MMP-2 and MMP-9 in Leishmania parasites, particularly in two different Leishmania species, L. amazonensis and L. braziliensis. The results show that MMP-9 has significantly higher gelatinolytic activity in L. amazonensis compared to L. braziliensis. The study suggests that MMP-2 and MMP-9 may play a role in the infection dynamics of these Leishmania species and could be potential markers for severe leishmaniasis and therapeutic targets.
Review
Pharmacology & Pharmacy
Amina T. Mneimneh, Mohammed M. Mehanna
Summary: Collagen-based scaffolds play a significant role in promoting regeneration, repair, and recovery of spinal cord injuries by providing a suitable environment for tissue repair, axonal regeneration, and vascularization. They are biocompatible, biodegradable, and widely used in pharmaceutical, cosmetic, food industries, and tissue engineering. Functionalized with different ligands and factors, collagen in scaffolds enhances its binding specificity and activity.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Orthopedics
Jeffrey R. Talarek, Alex N. Piacentini, Alexis C. Konja, Susumu Wada, Jacob B. Swanson, Samuel C. Nussenzweig, Joshua S. Dines, Scott A. Rodeo, Christopher L. Mendias
JOURNAL OF ORTHOPAEDIC RESEARCH
(2020)
Article
Physiology
Andrew C. Noah, Thomas M. Li, Leandro M. Martinez, Susumu Wada, Jacob B. Swanson, Nathaniel P. Disser, Kristoffer B. Sugg, Scott A. Rodeo, Theresa T. Lu, Christopher L. Mendias
JOURNAL OF APPLIED PHYSIOLOGY
(2020)
Article
Neurosciences
Nathaniel P. Disser, Gregory C. Ghahramani, Jacob B. Swanson, Susumu Wada, Max L. Chao, Scott A. Rodeo, David J. Oliver, Christopher L. Mendias
JOURNAL OF PHYSIOLOGY-LONDON
(2020)
Article
Orthopedics
Michael T. Curran, Asheesh Bedi, Christopher L. Mendias, Edward M. Wojtys, Megan Kujawa, Riann M. Palmieri-Smith
AMERICAN JOURNAL OF SPORTS MEDICINE
(2020)
Article
Orthopedics
Christopher L. Mendias, Elizabeth R. Sibilsky Enselman, Adam M. Olszewski, Jonathan P. Gumucio, Daniel L. Edon, Maxwell A. Konnaris, James E. Carpenter, Tariq M. Awan, Jon A. Jacobson, Joel J. Gagnier, Ariel L. Barkan, Asheesh Bedi
AMERICAN JOURNAL OF SPORTS MEDICINE
(2020)
Article
Orthopedics
Nathaniel P. Disser, Andrea J. De Micheli, Martin M. Schonk, Maxwell A. Konnaris, Alexander N. Piacentini, Daniel L. Edon, Brett G. Toresdahl, Scott A. Rodeo, Ellen K. Casey, Christopher L. Mendias
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME
(2020)
Article
Orthopedics
Drake G. LeBrun, Maxwell A. Konnaris, Gregory C. Ghahramani, Ajay Premkumar, Chris J. DeFrancesco, Jordan A. Gruskay, Aleksey Dvorzhinskiy, Milan S. Sandhu, Elan M. Goldwyn, Christopher L. Mendias, William M. Ricci
JOURNAL OF ORTHOPAEDIC TRAUMA
(2020)
Article
Cell Biology
Douglas W. Van Pelt, Yalda A. Kharaz, Dylan C. Sarver, Logan R. Eckhardt, Justin T. Dzierzawski, Nathaniel P. Disser, Alex N. Piacentini, Eithne Comerford, Brian McDonagh, Christopher L. Mendias
Summary: This study utilized multiomic approaches to investigate the pathological changes in Duchenne muscular dystrophy at the transcriptome, proteome, metabolome, and lipidome levels. Despite gross pathological changes in muscles of mdx/mTR mice, there were no differences in muscle fiber contractility compared to wild type mice. There was a moderate agreement between the proteome and transcriptome but also significant differences, with notable changes in markers of muscle metabolism observed in dystrophic muscles.
CONNECTIVE TISSUE RESEARCH
(2021)
Article
Medicine, Research & Experimental
Jonathan P. Gumucio, Martin M. Schonk, Yalda A. Kharaz, Eithne Comerford, Christopher L. Mendias
Article
Cell Biology
Andrea J. De Micheli, Jacob B. Swanson, Nathaniel P. Disser, Leandro M. Martinez, Nicholas R. Walker, David J. Oliver, Benjamin D. Cosgrove, Christopher L. Mendias
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2020)
Article
Anatomy & Morphology
Nathan S. Jeffery, Dylan C. Sarver, Christopher L. Mendias
Summary: This study investigates the competing spatial demands of brain and masticatory muscle growth within hypermuscular myostatin-deficient mouse model and computational simulations, exploring the network linking single genes to multiple phenotypic outcomes. Findings reveal different muscle and brain volume changes in myostatin-deficient mice at different growth stages, and simulations show skeletal morphological changes associated with muscle expansion and endocranial expansion.
JOURNAL OF ANATOMY
(2021)
Article
Biochemistry & Molecular Biology
Haiyin Li, Antonion Korcari, David Ciufo, Christopher L. L. Mendias, Scott A. A. Rodeo, Mark R. R. Buckley, Alayna E. E. Loiselle, Geoffrey S. S. Pitt, Chike Cao
Summary: This study found that Ca2+ signaling through Ca(V)1.2 voltage-gated Ca2+ channel plays a role in tendon formation. Ca(V)1.2 is highly expressed in developing tendons but downregulated in adult homeostasis. Mutant tendons with increased Ca2+ signaling display hypertrophic characteristics, increased collagen fibrillogenesis, and alterations in ECM proteins and growth factors.
Article
Sport Sciences
Jonathan S. Yu, David M. Dare, Daniel Edon, Alec L. Sinatro, Dylan C. Sarver, Scott Rodeo, Joshua S. Dines, Christopher L. Mendias
Summary: This exploratory study aimed to investigate the relationship between biomarkers of cartilage turnover and inflammation and specific shoulder lesions in patients with shoulder instability. The findings suggest that inflammatory biomarkers were not associated with specific shoulder lesions, while biomarkers of cartilage turnover were only elevated in Hill-Sachs lesions.
TRANSLATIONAL SPORTS MEDICINE
(2022)
Article
Orthopedics
Jordan A. Gruskay, Aleksey Dvorzhinskiy, Maxwell A. Konnaris, Drake G. LeBrun, Gregory C. Ghahramani, Ajay Premkumar, Christopher J. DeFrancesco, Christopher L. Mendias, William M. Ricci
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME
(2020)
Article
Physiology
Dylan C. Sarver, Kristoffer B. Sugg, Jeffrey R. Talarek, Jacob B. Swanson, David J. Oliver, Aaron C. Hinken, Henning F. Kramer, Christopher L. Mendias
PHYSIOLOGICAL REPORTS
(2019)