Article
Engineering, Civil
Marco Carlo Rampini, Giulio Zani, Matteo Colombo, Marco di Prisco
Summary: Textile-reinforced mortar (TRM) is a suitable engineering material for upgrading and retrofitting existing masonry and concrete structures subjected to seismic actions. Characterization activities have mainly focused on the material's monotonic behavior, while few studies have investigated its performance under cyclic actions. This study aims to investigate the uniaxial tensile behavior of cement-based composites reinforced with alkali-resistant glass fabrics and short dispersed polyvinyl alcohol fibers. The stiffness degradation during unloading-reloading cycles was quantified to evaluate damage evolution and provide analytical formulations for validating advanced nonlinear simulations.
JOURNAL OF COMPOSITES FOR CONSTRUCTION
(2023)
Article
Endocrinology & Metabolism
Hailey C. Cunningham, Sophie Orr, Deepa K. Murugesh, Allison W. Hsia, Benjamin Osipov, Lauren Go, Po Hung Wu, Alice Wong, Gabriela G. Loots, Galateia J. Kazakia, Blaine A. Christiansen
Summary: Mechanical unloading causes rapid loss of bone structure and strength, which gradually recovers after resuming normal loading. However, it is not well established how this adaptation to unloading and reloading changes with age. In this study, they examined bone adaptation during unloading and subsequent reloading in mice of different ages, and found important differences in bone adaptation between young and old mice, as well as osteocyte deficient mice. These findings suggest a period of vulnerability in skeletal health in older subjects during and following a period of disuse.
Article
Anatomy & Morphology
Emily R. Czajkowski, Marilyn Cisneros, Bianca S. Garcia, Jim Shen, Richard M. Cripps
Summary: Through proteomic analysis and gene knockdown experiments, a novel myofibrillar component CG1674 was identified in Drosophila muscle, which is essential for proper myofibril assembly and function in various muscle types. The localization and function of CG1674 are regulated by Z-disc components such as alpha-actinin, Mask, Zasp52, and Sals, highlighting its significance in muscle development.
DEVELOPMENTAL DYNAMICS
(2021)
Article
Biochemistry & Molecular Biology
Claire Camy, Thomas Brioche, Karim Senni, Alexandrine Bertaud, Cecile Genovesio, Edouard Lamy, Theo Fovet, Angele Chopard, Martine Pithioux, Sandrine Roffino
Summary: This study examined the effects of mechanical loading on the structure and mechanical properties of the Achilles tendon enthesis. Results showed that soleus atrophy was severe after hindlimb suspension, but calcified fibrocartilage was minimally affected. Non-calcified fibrocartilage surface area decreased, collagen fiber organization was disrupted, and collagen II distribution was altered. Reloading restored the fibrocartilage surface area and collagen II expression. Functionally, unloading resulted in decreased enthesis stiffness and a shift in the site of failure, while reloading restored the original elastic properties and site of failure. These findings have implications for countermeasure exercises during spaceflight.
Article
Engineering, Industrial
Hongli Hou, Guoqun Zhao, Liang Chen, Huiping Li
Summary: The study focused on the nonlinear anelastic behavior in unloading and unloading-reloading processes of severe plastic compressive deformation (SPCD) in hot-rolled copper plates along different directions. Experimental tests were conducted to analyze the unloading behavior, variation law of anelastic strain, and the effect of anisotropy. Models were established at micro and macro levels to accurately predict the anelastic strain and stress-strain relationship in SPCD.
JOURNAL OF MATERIALS PROCESSING TECHNOLOGY
(2021)
Article
Construction & Building Technology
Shuo Zhang, Mingpeng Liu, Chuan Wang, Hongbo Zhang, Jianqing Wu
Summary: This study investigates the mechanical properties of silt-based foamed concrete under uniaxial loading conditions and the impact of wet density and silt content on its performance. The results show that foamed concrete exhibits distinct elastoplastic characteristics under uniaxial compression loading and resilient ability even at large strains. Density contributes significantly to strength formation, while silt content diversely affects the strength parameters of foamed concrete.
CONSTRUCTION AND BUILDING MATERIALS
(2022)
Article
Biology
Pankaj Pathak, Yotam Blech-Hermoni, Kalpana Subedi, Jessica Mpamugo, Charissa Obeng-Nyarko, Rachel Ohman, Ilda Molloy, Malcolm Kates, Jessica Hale, Stacey Stauffer, Shyam K. Sharan, Ami Mankodi
Summary: This research demonstrates that the LDB3 protein regulates mechanical stress signaling through interactions with filamin C, HSPA8, and PKC alpha. Mutations in LDB3 lead to early aggregation of filamin C and its chaperones at the muscle Z-disc, ultimately causing protein aggregation myopathy.
COMMUNICATIONS BIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Renata Mazurek, Taro Kariya, Tomoki Sakata, Spyros A. Mavropoulos, Anjali J. Ravichandran, Francisco J. Romeo, Kelly P. Yamada, Kiyotake Ishikawa
Summary: We evaluated the hemodynamic and cellular effects of reloading after Impella-mediated LV unloading in Yorkshire pigs and investigated the optimal exit strategy post-MI. Acute reloading caused an immediate rise in end-diastolic wall stress and increased cardiomyocyte apoptosis. Although the MI study did not yield statistically significant findings, the numerically smaller infarct size and no-reflow area in the gradual reloading group warrant further examination of this reloading approach.
JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH
(2023)
Article
Materials Science, Multidisciplinary
X. X. Zhang, H. Andrae, S. Harjo, W. Gong, T. Kawasaki, A. Lutz, M. Lahres
Summary: In-situ neutron diffraction was used to investigate the residual strains, stresses, and dislocation density in LPBF AlSi10Mg during loading-unloading-reloading deformation. The study revealed that the AlSi10Mg alloy exhibits notable dislocation annihilation phenomenon in the Al matrix during unloading stages, driven by compressive residual stresses.
MATERIALS & DESIGN
(2021)
Article
Food Science & Technology
Shan Shang, Bingcun Wu, Baoshang Fu, Pengfei Jiang, Ying Liu, Libo Qi, Ming Du, Xiuping Dong
Summary: This study developed a simple and effective strategy to improve the tenderness of puffer fish muscle through papain treatment. The results showed that the hardness of the fish muscle decreased with increasing papain concentration, indicating improved tenderness. Protein analysis confirmed the degradation of myosin and actin into low molecular weight peptides with more disorder structures. These findings are important for improving the quality of puffer fish muscle.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Bin Ma, Xinchao Ding, Xingzhou Chen
Summary: The excavation-unloading damage effects of western high-geostress slopes on rock were investigated using sandstone reloading mechanical tests under pre-peak confining pressure unloading. The deformation and failure mechanisms were analyzed using the particle discrete-element method. (1) As unloading failure approached, increasing confining pressure led to a decrease in specimen bearing capacity. (2) Unloading of confining pressure promoted microdefect propagation and development, with specimens rapidly reaching the damage stress value after reaching the initiation stress value. Penetration fracture zones were more pronounced and expansive in the model, with dense crack areas concentrated in the fracture zone and surrounding area. (3) The tangential contact force exhibited the highest average interval in the direction of crack expansion and propagation. Strong force chains primarily bore external loads, while weak force chains played a key role in maintaining stability. (4) The number of cracks developing during the confining pressure unloading damage process indicated that the loading process did not cause damage to the specimens. Fracture zones further propagated and formed on dominant fractures due to the damage caused by confining pressure unloading disturbance.
APPLIED SCIENCES-BASEL
(2023)
Article
Mechanics
B. Zhang, T. Yu, J. G. Teng
Summary: This paper presents a systematic experimental study on the cyclic stress-strain behavior of concrete in hybrid DSTCs under different loading conditions, investigating the cumulative effect of loading history on stress deterioration and plastic strain of the concrete. It also compares test results with predictions of an existing cyclic stress-strain model, demonstrating the model's accuracy in predicting the complete cyclic stress-strain curve of concrete in hybrid DSTCs.
COMPOSITE STRUCTURES
(2021)
Article
Nanoscience & Nanotechnology
Hongli Hou, Guoqun Zhao, Junquan Yu, Dejin Wei
Summary: The unloading-reloading behavior of hot compressive deformed aluminum alloys at elevated temperature was studied from both macroscopic and microscopic perspectives, revealing the effects of deformation temperature, strain rate, and strain. A macroscopic model taking into account strain, strain rate, and temperature was established to predict the unloading-reloading process. A physically-based micro-model was constructed to explain the physical mechanism of stress relaxation and inelastic strain recovery.
MATERIALS SCIENCE AND ENGINEERING A-STRUCTURAL MATERIALS PROPERTIES MICROSTRUCTURE AND PROCESSING
(2023)
Article
Chemistry, Multidisciplinary
Yongjian Zhu, Peng Li, Ping Wang, Chengcheng Mei, Heng Ren, Xizhi Wang
Summary: The mining of deep coal resources occurs in a high-stress geological environment as well as an engineering environment of rock excavation and unloading. Research on the re-bearing capacity characteristics and damage mechanism of rock masses damaged by peak front unloading is critical in revealing the destabilization and rupture law of deep rock bodies.
APPLIED SCIENCES-BASEL
(2022)
Article
Materials Science, Multidisciplinary
Philip Manuel Pohl, Moritz Kuglstatter, Mathias Goeken, Heinz Werner Hoeppel
Summary: Heterostructured materials, such as metallic laminates (LMCs), can exhibit enhanced mechanical behavior through the hetero-deformation-induced (HDI) strengthening effect. This study introduces a novel approach to quantify the co-deformation effects in metallic laminates by analyzing the behavior of inelastic back strain upon unloading. Experimental tests reveal additional amounts of inelastic back strain for different metallic laminate systems compared to a linear rule of mixture approximation. Finite element analysis provides insights into the distribution of residual plastic strain in the metallic laminates, supporting the derived schematic overview of the spatial distribution of the hetero-deformation zone. The method presented in this study enables valuable insights into the cyclic deformation behavior of metallic laminates for future applications.
Editorial Material
Cell & Tissue Engineering
Ryo Fujita, Colin Crist
Article
Cell Biology
Kiyoshi Yoshioka, Ryo Fujita, Daiki Seko, Takashi Suematsu, Shinji Miura, Yusuke Ono
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2019)
Article
Cell & Tissue Engineering
Shota Kurotsu, Taketaro Sadahiro, Ryo Fujita, Hidenori Tani, Hiroyuki Yamakawa, Fumiya Tamura, Mari Isomi, Hidenori Kojima, Yu Yamada, Yuto Abe, Yoshiko Murakata, Tatsuya Akiyama, Naoto Muraoka, Ichiro Harada, Takeshi Suzuki, Keiichi Fukuda, Masaki Ieda
Article
Cell & Tissue Engineering
Daiki Seko, Ryo Fujita, Yuriko Kitajima, Kodai Nakamura, Yuuki Imai, Yusuke Ono
Article
Developmental Biology
Ryo Fujita, Solene Jamet, Graham Lean, Harry Chun Man Cheng, Steven Hebert, Claudia L. Kleinman, Colin Crist
Summary: Translation control of gene expression through P-eIF2α enables the expansion of satellite cells. Certain genes are upregulated at the protein level without corresponding changes at the mRNA level. Deficiency of Tacc3 results in defects in the expansion, self-renewal, and regeneration of skeletal muscle in satellite cells.
Article
Biochemistry & Molecular Biology
Mari Isomi, Taketaro Sadahiro, Ryo Fujita, Yuto Abe, Yu Yamada, Tatsuya Akiyama, Hiroaki Mizukami, Tsugumine Shu, Keiichi Fukuda, Masaki Ieda
Summary: Adult hearts have limited regenerative capacity, but direct reprogramming of cardiac fibroblasts into induced cardiomyocytes with GMT may have significant potential for cardiac repair. Studies have shown that SeV-GMT reprogramming in mouse hearts after acute MI can improve cardiac function.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Letter
Cardiac & Cardiovascular Systems
Mari Isomi, Taketaro Sadahiro, Hiroyuki Yamakawa, Ryo Fujita, Yu Yamada, Yuto Abe, Yoshiko Murakata, Tatsuya Akiyama, Tsugumine Shu, Hiroaki Mizukami, Keiichi Fukuda, Masaki Ieda
Article
Biology
Takuto Hayashi, Takashi Kudo, Ryo Fujita, Shin-ichiro Fujita, Hirona Tsubouchi, Sayaka Fuseya, Riku Suzuki, Michito Hamada, Risa Okada, Masafumi Muratani, Dai Shiba, Takafumi Suzuki, Eiji Warabi, Masayuki Yamamoto, Satoru Takahashi
Summary: The study shows that NRF2 plays a key role in maintaining slow-type muscle fibers in the soleus muscle during space flight, impacting the transition of muscle fiber types. These findings provide important insights into the mechanism of skeletal muscle atrophy in microgravity environments.
COMMUNICATIONS BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Nicole Baker, Steven Wade, Matthew Triolo, John Girgis, Damian Chwastek, Sarah Larrigan, Peter Feige, Ryo Fujita, Colin Crist, Michael A. Rudnicki, Yan Burelle, Mireille Khacho
Summary: Physiological changes in mitochondrial shape play a crucial role in regulating the quiescent state and activation potential of adult stem cells. Mitochondrial fragmentation promotes the exit from deep quiescence, while loss of the mitochondrial fusion protein OPA1 leads to premature activation and depletion of stem cells.
Article
Cell Biology
Charlotte Senechal, Ryo Fujita, Solene Jamet, Arhamatoulaye Maiga, Junio Dort, Zakaria Orfi, Nicolas A. Dumont, Michel Bouvier, Colin Crist
Summary: Skeletal muscle contains a pool of quiescent muscle stem cells (MuSCs) that can regenerate the tissue after injury. This study shows that the adhesion G-protein-coupled receptor Gpr116 plays a crucial role in maintaining the long-term MuSC pool. Gpr116 is highly expressed in quiescent MuSCs but rapidly down-regulated upon activation, and its deficiency leads to progressive depletion and impaired self-renewal of MuSCs. Stimulation of MuSCs with the GPR116 Stachel peptide delays activation and differentiation, and the interaction between GPR116 and β-arrestin1 in the nucleus regulates gene expression to maintain the MuSC pool.
Article
Cell Biology
Shunya Sadaki, Ryo Fujita, Takuto Hayashi, Ayano Nakamura, Yui Okamura, Sayaka Fuseya, Michito Hamada, Eiji Warabi, Akihiro Kuno, Akiko Ishii, Masafumi Muratani, Risa Okada, Dai Shiba, Takashi Kudo, Shin'ichi Takeda, Satoru Takahashi
Summary: Myofibers can be broadly classified into two types, fatigue-resistant slow-twitch fibers and rapidly fatiguing fast-twitch fibers. This study investigates the molecular regulation of fast-twitch muscle and identifies the large Maf transcription factor family as a major regulator for the determination of type IIb myofibers. The ablation of these transcription factors leads to a significant loss of type IIb myofibers, resulting in enhanced endurance capacity and reduced muscle force.
Article
Multidisciplinary Sciences
Ryo Fujita, Seiya Mizuno, Taketaro Sadahiro, Takuto Hayashi, Takehito Sugasawa, Fumihiro Sugiyama, Yusuke Ono, Satoru Takahashi, Masaki Ieda
Summary: Researchers have developed a MyoD knock-in (MyoD-KI) mouse model that allows for monitoring the dynamic changes of MyoD protein in muscle stem cells (MuSCs). This model provides a valuable tool for studying the fate determination and heterogeneity of MuSCs, as well as for drug screening in stem cell therapy.
Article
Biology
Takuto Hayashi, Ryo Fujita, Risa Okada, Michito Hamada, Riku Suzuki, Sayaka Fuseya, James Leckey, Maho Kanai, Yuri Inoue, Shunya Sadaki, Ayano Nakamura, Yui Okamura, Chikara Abe, Hironobu Morita, Tatsuya Aiba, Teruhiro Senkoji, Michihiko Shimomura, Maki Okada, Daisuke Kamimura, Akane Yumoto, Masafumi Muratani, Takashi Kudo, Dai Shiba, Satoru Takahashi
Summary: Using the MARS system, we studied the effects of different gravitational levels on skeletal muscle in mice. Lunar gravity was found to prevent muscle atrophy, but failed to prevent myofiber type transition. Our study suggests that different gravitational thresholds may be required for skeletal muscle adaptation.
COMMUNICATIONS BIOLOGY
(2023)
Article
Hematology
Tatsuya Akiyama, Taketaro Sadahiro, Yu Yamada, Ryo Fujita, Yuto Abe, Koji Nakano, Seiichiro Honda, Masatsugu Ema, Yoshiaki Kubota, Satoshi Sakai, Nobuyuki Hizawa, Masaki Ieda
Summary: In this study, the differential expression patterns of Flk1 and Flt1 in pulmonary endothelial cells were investigated. Deficiency of Flk1 and hypoxia jointly dysregulated arteriolar endothelial cells, promoting vascular remodeling. Therefore, dysfunctional Flk1+ endothelial cells may contribute to the pathogenesis of vascular remodeling in pulmonary arterioles.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Hidenori Tani, Taketaro Sadahiro, Yu Yamada, Mari Isomi, Hiroyuki Yamakawa, Ryo Fujita, Yuto Abe, Tatsuya Akiyama, Koji Nakano, Yuta Kuze, Masahide Seki, Yutaka Suzuki, Manabu Fujisawa, Mamiko Sakata-Yanagimoto, Shigeru Chiba, Keiichi Fukuda, Masaki Ieda
Summary: Adult cardiomyocytes have limited regenerative ability, and the synthesis of extracellular matrix by cardiac fibroblasts (CFs) results in fibrosis and heart dysfunction after myocardial infarction (MI). The overexpression of cardiac transcription factors, MGTH, can directly reprogram CFs into induced cardiomyocytes (iCMs) and improve cardiac function in acute MI. However, it is unclear whether in vivo cardiac reprogramming can repair chronic MI with established scars.
