期刊
JOURNAL OF APPLIED PHYSIOLOGY
卷 111, 期 3, 页码 929-937出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/japplphysiol.00541.2011
关键词
stem cells; cardiovascular disease; physical activity
资金
- National Institutes of Health [T32-AG-000268]
- National Heart, Lung, and Blood Institute [R25-HL-092604]
- Department of Veterans Affairs [CDA-2-0039]
- Baltimore Veterans Affairs Medical Center Geriatric Research, Education, and Clinical Center
- University of Maryland's Kinesiology Graduate Research Initiative
Jenkins NT, Landers RQ, Prior SJ, Soni N, Spangenburg EE, Hagberg JM. Effects of acute and chronic endurance exercise on intracellular nitric oxide and superoxide in circulating CD34(+) and CD34(+) cells. J Appl Physiol 111: 929-937, 2011. First published June 23, 2011; doi: 10.1152/japplphysiol.00541.2011.-We investigated the influence of acute and chronic endurance exercise on levels of intracellular nitric oxide (NO), superoxide (O-2(center dot-)), and expression of genes regulating the balance between these free radicals in CD34(+) and CD34(+) peripheral blood mononuclear cells (PBMCs; isolated by immunomagnetic cell separation). Blood samples were obtained from age-and body mass index (BMI)-matched endurance-trained (n = 10) and sedentary (n = 10) men before and after 30 min of exercise at 75% maximal oxygen uptake ((V) over doto(2max)). Baseline levels of intracellular NO (measured by DAF-FM diacetate) and O-2(center dot-) (measured by dihydroethidium) were 26% (P < 0.05) and 10% (P < 0.05) higher, respectively, in CD34(+) PBMCs from the sedentary group compared with the endurance-trained group. CD34(+) PBMCs from the sedentary group at baseline had twofold greater inducible nitric oxide synthase (iNOS) mRNA and 50% lower endothelial NOS (eNOS) mRNA levels compared with the trained group (P < 0.05). The baseline group difference in O-2(center dot-) was eliminated by acute exercise. Experiments with apocynin indicated that the training-related difference in O-2(center dot-) levels was explained by increased NADPH oxidase activity in the sedentary state. mRNA levels of additional angiogenic and antioxidant genes were consistent with a more angiogenic profile in CD34(+) cells of trained subjects. CD34(+) PBMCs, examined for exploratory purposes, also displayed a more angiogenic mRNA profile in trained subjects, with vascular endothelial growth factor (VEGF) and eNOS being more highly expressed in trained subjects. Overall, our data suggest an association between the sedentary state and increased nitro-oxidative stress in CD34(+) cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据