High-throughput analysis and protein engineering using microcapillary arrays

Massively parallel delivery of large cargo into mammalian cells with light pulses

(2015) Yi-Chien Wu et al.   NATURE METHODS

Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange

(2015) Yidan Ding et al.   NATURE METHODS

Dissecting enzyme function with microfluidic-based deep mutational scanning

(2015) Philip A. Romero et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Exploiting Highly Ordered Subnanoliter Volume Microcapillaries as Microtools for the Analysis of Antibody Producing Cells

(2014) Valerie Fitzgerald et al.   ANALYTICAL CHEMISTRY

One in a Million: Flow Cytometric Sorting of Single Cell-Lysate Assays in Monodisperse Picolitre Double Emulsion Droplets for Directed Evolution

(2014) Anastasia Zinchenko et al.   ANALYTICAL CHEMISTRY

Probing the Origins of Catalytic Discrimination between Phosphate and Sulfate Monoester Hydrolysis: Comparative Analysis of Alkaline Phosphatase and Protein Tyrosine Phosphatases

(2014) Logan D. Andrews et al.   BIOCHEMISTRY

Ultra-High-Throughput Screening Method for the Directed Evolution of Glucose Oxidase

(2014) Raluca Ostafe et al.   CHEMISTRY & BIOLOGY

A Single Molecule Perspective on the Functional Diversity of in Vitro Evolved β-Glucuronidase

(2014) Raphaela B. Liebherr et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Realizing the promise of cancer predisposition genes

(2014) Nazneen Rahman   NATURE

An engineered Axl 'decoy receptor' effectively silences the Gas6-Axl signaling axis

(2014) Mihalis S Kariolis et al.   Nature Chemical Biology

Evolution of enzyme catalysts caged in biomimetic gel-shell beads

(2014) Martin Fischlechner et al.   Nature Chemistry

Engineering and characterizing monomeric fluorescent proteins for live-cell imaging applications

(2014) Hui-wang Ai et al.   Nature Protocols

Micropallet arrays for the capture, isolation and culture of circulating tumor cells from whole blood of mice engrafted with primary human pancreatic adenocarcinoma

(2013) Philip C. Gach et al.   BIOSENSORS & BIOELECTRONICS

Engineered thermostable fungal Cel6A and Cel7A cellobiohydrolases hydrolyze cellulose efficiently at elevated temperatures

(2013) Indira Wu et al.   BIOTECHNOLOGY AND BIOENGINEERING

A Fluorogenic Red Fluorescent Protein Heterodimer

(2012) Spencer C. Alford et al.   CHEMISTRY & BIOLOGY

Olefin Cyclopropanation via Carbene Transfer Catalyzed by Engineered Cytochrome P450 Enzymes

(2012) P. S. Coelho et al.   SCIENCE

Dimerization-Dependent Green and Yellow Fluorescent Proteins

(2012) Spencer C. Alford et al.   ACS Synthetic Biology

Rational Engineering of Enzyme Allosteric Regulation through Sequence Evolution Analysis

(2012) Jae-Seong Yang et al.   PLoS Computational Biology

Initial impact of the sequencing of the human genome

(2011) Eric S. Lander   NATURE

Removing allosteric feedback inhibition of tomato 4-coumarate:CoA ligase by directed evolution

(2011) Moti Alberstein et al.   PLANT JOURNAL

A general strategy for the evolution of bond-forming enzymes using yeast display

(2011) I. Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Engineering of a fungal β-galactosidase to remove product inhibition by galactose

(2010) Xuejun Hu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Sequence space and the ongoing expansion of the protein universe

(2010) Inna S. Povolotskaya et al.   NATURE

Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations

(2010) David M Rissin et al.   NATURE BIOTECHNOLOGY

Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

(2010) J. J. Agresti et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Accuracy and precision in quantitative fluorescence microscopy

(2009) Jennifer C. Waters   JOURNAL OF CELL BIOLOGY

Mechanistic Aspects of Horseradish Peroxidase Elucidated through Single-Molecule Studies

(2009) Hans H. Gorris et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Exploring protein fitness landscapes by directed evolution

(2009) Philip A. Romero et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Comparative Enzymology in the Alkaline Phosphatase Superfamily to Determine the Catalytic Role of an Active-Site Metal Ion

(2008) Jesse G. Zalatan et al.   JOURNAL OF MOLECULAR BIOLOGY