Article
Biochemistry & Molecular Biology
Junhao Cheng, Marhaba Ahmat, Henan Guo, Xubiao Wei, Lulu Zhang, Qiang Cheng, Jing Zhang, Junyong Wang, Dayong Si, Yueping Zhang, Rijun Zhang
Summary: This study successfully established an efficient production strategy for CLP and evaluated interference factors affecting the biological activity of CLP, paving the way for further exploration in the treatment of multidrug-resistant bacterial infections.
Article
Microbiology
Runzhe Wu, Xunxi Dong, Qiang Wang, Zirui Zhang, Jianhua Wang, Xiao Wang
Summary: The study synthesized D1018M and D1018 peptides with improved activity and found that they exhibited enhanced resistance against proteases; D1018M showed slightly increased antimicrobial activity, and these peptides had low cytotoxicity, strong inhibitory effects on E. coli, and excellent inhibition capacity against LPS-induced proinflammation response.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Jingyi Dai, Ritian Jin, Jialong Gao, Jude Juventus Aweya, Rong Lin, Guiling Li, Shen Yang
Summary: The rising threat of antibiotic-resistant pathogens has led to the exploration of naturally-derived antimicrobial peptides (AMPs) as replacements for chemical preservatives in foodborne pathogen control. In this study, ten peptides were discovered from salt-fermented shrimps via ultra-performance liquid chromatography-mass spectrometry. Among them, PV-Q5 exhibited strong antibacterial activity against Vibrio parahaemolyticus and Escherichia coli, with a minimum inhibitory concentration of 31.25 μg/mL. PV-Q5 also demonstrated an ability to increase bacterial cell membrane permeability and rupture bacterial cell membranes. These findings suggest that PV-Q5 could be used in food preservation to effectively control foodborne pathogenic bacteria.
Article
Biotechnology & Applied Microbiology
Sima Sadat Seyedjavadi, Mehdi Razzaghi-Abyaneh, Mohammad Javad Nasiri, Ali Hashemi, Hossein Goudarzi, Mehrdad Haghighi, Masoud Dadashi, Mehdi Goudarzi, Hadi Zare-Zardini, Behzad Pourhossein
Summary: A novel antimicrobial peptide called dendrocin-ZM1 was isolated from Zataria multiflora Boiss, showing broad-spectrum antibacterial activity against various strains with low hemolysis and cytotoxicity. Dendrocin-ZM1 exhibited good heat stability and activity in a wide pH range.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2022)
Article
Infectious Diseases
Yi Wang, Yuchi Song, Shaonan Yan, Rina Hiramine, Yuki Ohnishi, Yuki Yokoi, Kiminori Nakamura, Takashi Kikukawa, Tokiyoshi Ayabe, Tomoyasu Aizawa
Summary: This study investigated the antibacterial mechanisms of Cryptdin-4 in different forms (oxidized and reduced states) under different oxygen conditions using Escherichia coli as a model. The results showed that both forms exhibited antimicrobial activity against bacteria under aerobic conditions through rapid membrane depolarization. Under anaerobic conditions, the ability to disrupt bacterial membrane function decreased, but the reduced form still maintained some antimicrobial activity, possibly through DNA binding inhibition of intracellular functions.
Article
Microbiology
Lingling Zhou, Kaiqi Lian, Mengting Wang, Xueyi Jing, Yuanchen Zhang, Jinling Cao
Summary: This study demonstrated that LL-1 has a strong bactericidal effect on E. coli by increasing the permeability of the cell membrane, resulting in leakage of intracellular content. This finding is of great importance for further understanding the antibacterial mechanism of LL-1 against E. coli and its clinical applications.
Article
Infectious Diseases
Raffaella Scotti, Bruno Casciaro, Annarita Stringaro, Fabrizio Morgia, Maria Luisa Mangoni, Roberta Gabbianelli
Summary: New strategies are needed to fight against multidrug-resistant bacteria caused by the overuse of antibiotics. Antibiofilm peptides derived from amphibian skin, such as Esc(1-21) and Esc(1-18), have shown potential to treat infections by inhibiting bacterial growth and biofilm formation. These peptides also induce the expression of genes associated with biofilm regulation and stress response in E. coli O157:H7.
Review
Biochemistry & Molecular Biology
Tianxi Li, Lulu Li, Fangyuan Du, Lei Sun, Jichao Shi, Miao Long, Zeliang Chen
Summary: This review focuses on antifungal peptides isolated from various microorganisms, outlining their antifungal activity, toxic effects, and mechanisms of action, as well as common solutions to mitigate their toxicity. This work serves as a useful reference for further clinical research and the development of safe antifungal drugs with high efficiencies and broad application spectra.
Article
Biochemistry & Molecular Biology
Dongdong Li, Pingle Liu, Fang Hao, Yang Lv, Wei Xiong, Cheng Yan, Yi Wu, Hean Luo
Summary: In this study, a hybrid material silver/chitosan-sepiolite (Ag/5CTs-Sep) with antimicrobial properties was successfully prepared. The material exhibited high inhibitory effects against Staphylococcus aureus, Escherichia coli, and Aspergillus niger. The antimicrobial mechanism was revealed through characterization, and the material showed low cytotoxicity. Moreover, the material was found to be suitable for water-based coatings and met the performance standards.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Fisheries
Rui Xue, Yijing Han, Fan Li, Lizhu Chen, Dinglong Yang, Jianmin Zhao
Summary: In this study, two maclins were identified from clam Venerupis philippinarum, showing significant antipathogenic properties and bactericidal effects. VpMacin-2 exhibited superior broad-spectrum antimicrobial activities and biofilm inhibition compared to VpMacin-1, suggesting their important roles in host defense mechanisms.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Immunology
Nadghia F. Leite-Sampaio, Cicera Natalia F. L. Gondim, Celestina E. Sobral de Souza, Henrique D. M. Coutinho
Summary: This study investigated the antibacterial and antimicrobial modulating activity of alpha-pinene and borneol against Escherichia coli and enteropathogenic and enterotoxigenic serotypes. The results showed that alpha-pinene and borneol exhibited low antimicrobial action against multidrug-resistant Escherichia coli, but no antimicrobial activity was observed against enteropathogenic and enterotoxigenic serotypes. A synergistic action of borneol with ciprofloxacin against enterotoxigenic serotypes was noted.
MICROBIAL PATHOGENESIS
(2022)
Article
Chemistry, Multidisciplinary
Monica Paesa, Fernando Almazan, Cristina Yus, Victor Sebastian, Manuel Arruebo, Luis M. Gandia, Santiago Reinoso, Ismael Pellejero, Gracia Mendoza
Summary: This study investigates the antimicrobial performance of polyoxometalate-stabilized gold nanoparticles against Escherichia coli and Staphylococcus aureus, finding that one particular composite exhibits excellent bactericidal efficiency without harming mammalian cells.
Article
Microbiology
Xinghong Zhao, Xinyi Zhong, Shinong Yang, Kai Deng, Lu Liu, Xu Song, Yuanfeng Zou, Lixia Li, Xun Zhou, Renyong Jia, Juchun Lin, Huaqiao Tang, Gang Ye, Jianqing Yang, Shan Zhao, Yifei Lang, Hongping Wan, Zhongqiong Yin, Oscar P. Kuipers
Summary: Brevicidine, a bacterial cyclic lipopeptide, exhibits potent and selective antimicrobial activity against Gram-negative pathogens. However, little was known about its mechanism of action before our investigations. We found that brevicidine exerts its bactericidal activity by interacting with lipopolysaccharide, targeting phosphatidylglycerol and cardiolipin, and disrupting the proton motive force of bacteria.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Food Science & Technology
Haotian Deng, Jinyan Zhu, Yuqi Tong, Yanwen Kong, Chang Tan, Mingyue Wang, Meizhi Wan, Xianjun Meng
Summary: Aronia melanocarpa anthocyanins (AMAs) exhibit strong antibacterial activity against Escherichia coli by damaging the cell wall and membrane structure of the bacteria. By targeting multiple cellular components, AMAs have the potential to be used as natural food preservatives.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Acoustics
Shen Yang, Zijin Yuan, Jude Juventus Aweya, Shiying Huang, Shanggui Deng, Linfan Shi, Mingjing Zheng, Yueling Zhang, Guangming Liu
Summary: This study found that the in silico predicted antimicrobial peptide TGH2 combined with low-intensity ultrasound significantly reduced the minimum inhibitory concentration of Escherichia coli and enhanced bactericidal effect, as shown by transmission electron microscopy and circular dichroism for structural analysis.
ULTRASONICS SONOCHEMISTRY
(2021)
Review
Chemistry, Multidisciplinary
Tudor Luchian, Yoonkyung Park, Alina Asandei, Irina Schiopu, Loredana Mereuta, Aurelia Apetrei
ACCOUNTS OF CHEMICAL RESEARCH
(2019)
Article
Chemistry, Multidisciplinary
Alina Asandei, Loredana Mereuta, Jonggwan Park, Chang Ho Seo, Yoonkyung Park, Tudor Luchian
Article
Chemistry, Analytical
Loredana Mereuta, Alina Asandei, Irina Schiopu, Yoonkyung Park, Tudor Luchian
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Analytical
Isabela S. Dragomir, Ioana C. Bucataru, Irina Schiopu, Tudor Luchian
ANALYTICAL CHEMISTRY
(2020)
Article
Multidisciplinary Sciences
Su Jin Ko, Eunji Park, Alina Asandei, Jee-Young Choi, Seung-Chul Lee, Chang Ho Seo, Tudor Luchian, Yoonkyung Park
SCIENTIFIC REPORTS
(2020)
Article
Nanoscience & Nanotechnology
Alina Asandei, Loredana Mereuta, Irina Schiopu, Jonggwan Park, Chang Ho Seo, Yoonkyung Park, Tudor Luchian
ACS APPLIED MATERIALS & INTERFACES
(2020)
Article
Polymer Science
Isabela S. Dragomir, Alina Asandei, Irina Schiopu, Ioana C. Bucataru, Loredana Mereuta, Tudor Luchian
Summary: Utilizing nanopore tweezing technique to capture amino acid-functionalized peptide nucleic acids, this study correlated the stochastic fluctuations of ionic current through nanopores with the composition and order of bases in PNAs, revealing insights into the conformational dynamics and improving the recognition capability of single-molecule sequencing.
Review
Biochemical Research Methods
Tudor Luchian, Loredana Mereuta, Yoonkyung Park, Alina Asandei, Irina Schiopu
Summary: Over the past 30 years, DNA nanotechnology has made significant advancements through the combination of detection and discovery of DNA, leading to the emergence of novel nucleic acids-based biopolymers. The development of nanopores for fast and direct detection of various molecules revolutionized technological evolution, enabling DNA detection and sequencing at the single-molecule level. Nanopore-based tools have proven versatile in biomedicine, chemistry, biology, and physics, with applications spanning materials science to ecology and anthropology.
Article
Biochemical Research Methods
Alina Asandei, Loredana Mereuta, Irina Schiopu, Yoonkyung Park, Tudor Luchian
Summary: The method described utilizes capacitive current transmembrane technology for the in vitro detection of the SARS-CoV-2 (S-1) spike protein subunit, with real-time spectral analysis of the second harmonic amplitude of the capacitive current allowing observation of changes in membrane potential induced by interactions with the S-1 protein subunit. This approach offers advantages in terms of low cost, quick implementation, and potential for early disease diagnosis.
Article
Chemistry, Multidisciplinary
Alina Asandei, Loredana Mereuta, Ioana C. Bucataru, Yoonkyung Park, Tudor Luchian
Summary: To alleviate solubility-related shortcomings of neutral peptide nucleic acids (PNA), the incorporation of charged sidechains onto PNA structure is a powerful strategy. This study uses a single-molecule technique and demonstrates that the ionic strength of the electrolyte significantly affects the ionic current blockade signature of poly(Arg)-PNAs interacting with nanopores. High salt-containing electrolytes enhance the capture and isolation of PNA-DNA complexes, while low salt electrolytes impede the specific hybridization of cationic PNAs with complementary ssDNAs. The reduced charge screening in low salt electrolytes enables non-specific electrostatic interactions, reducing the ability of PNA-DNA complementary association.
CHEMISTRY-AN ASIAN JOURNAL
(2022)
Article
Chemistry, Analytical
Loredana Mereuta, Alina Asandei, Isabela Dragomir, Jonggwan Park, Yoonkyung Park, Tudor Luchian
Summary: This study introduces an exploratory single-molecule approach for multiplexed discrimination among similar-sized single-stranded DNAs by combining short arginine tags with peptide nucleic acid probes and alpha-hemolysin nanopore. The detection of ssDNA targets was enabled by distinctive molecular processes associated with nanopore-mediated duplexes capture, trapping, and unzipping, marked by the length-variable poly-Arg tail.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Ioana C. Bucataru, Isabela Dragomir, Alina Asandei, Ana-Maria Pantazica, Alina Ghionescu, Norica Branza-Nichita, Yoonkyung Park, Tudor Luchian
Summary: This study proposes a method for detecting the chronic hepatitis B virus e-antigen using resistive-pulse sensing technology in conjunction with the alpha-hemolysin protein nanopore. The recognition between the virus antigen and an antibody was detected through transient ionic current spikes generated by protein aggregates near the nanopore. Despite the inability to capture the antigen or antibody inside the nanopore, protein subpopulations in the solution could be detected.
Article
Nanoscience & Nanotechnology
Loredana Mereuta, Alina Asandei, Irina Schiopu, Jonggwan Park, Yoonkyung Park, Tudor Luchian
Summary: Nanopores offer sensitive and cost-effective single-molecule sensing capabilities, and the advent of nanopore-based DNA detection and sequencing technologies has had a significant societal impact. To address the challenge of forming stable, long-lasting single nanopores, we have developed a new approach for detecting small single-stranded DNA using dynamic hybrid constructs.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Loredana Mereuta, Alina Asandei, Ioan Andricioaei, Jonggwan Park, Yoonkyung Park, Tudor Luchian
Summary: This study presents a method to control the passage of captured molecules in a nanopore by manipulating the electrostatic interactions between single-stranded DNAs and low pH-induced charges. The findings suggest that this pH-dependent tethering effect has the potential to provide a simple read-out of nucleotide sequences.
Article
Chemistry, Physical
Alina Asandei, Giovanni Di Muccio, Irina Schiopu, Loredana Mereuta, Isabela S. Dragomir, Mauro Chinappi, Tudor Luchian