期刊
JOURNAL OF APPLIED PHYCOLOGY
卷 26, 期 5, 页码 2087-2095出版社
SPRINGER
DOI: 10.1007/s10811-014-0242-5
关键词
Microalgae; Botryococcus braunii; Culture media; Biomass; Exopolysaccharides; FTIR
资金
- Empresas Publicas de Medellin (EPM)
- University of Antioquia
- Division of Sustainability of the Committee for Research Development of the Universidad de Antioquia - CODI
Botryococcus braunii is a colonial green microalga with recognized potential to synthesize lipids and hydrocarbons for biofuel production. Besides this ability, this microalga also produces exopolysaccharides (EPS). Nevertheless, there are few reports about their biotechnological aspects and industrial applications. In this study, the effect of the nutritional conditions was examined by using two different culture media (BG11 and D medium). To our knowledge, the latter has not been reported before for culturing B. braunii. After 49 days of incubation, the final production of EPS was found to be statistically higher (P < 0.05) in the D medium (0.549 +/- 0.044 g L-1) than in BG11 (0.336 +/- 0.009 g L-1). On the contrary, the biomass production was found to be higher in BG11 (1.019 +/- 0.051 g L-1) than in the D medium (0.953 +/- 0.056 g L-1). However, this difference was not statistically significant. The difference in salinity and nitrogen concentration between both media is suggested as the main factor involved in the EPS and biomass results. FTIR spectra of B. braunii EPS from both media revealed presence of uronic acids and absence of amino and sulfate groups. Despite the similarity between both spectra, there were some different signals (at 1,921.52 and 720.60 cm(-1)) which may mean a difference in glycosyl composition.
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