Article
Plant Sciences
Kuang-Teng Wang, Ming-Chang Hong, Yu-Sheng Wu, Tsung-Meng Wu
Summary: Duckweed is one of the smallest flowering plants in the world and is used for livestock as biofactors and feedstuff additives due to its high reproduction rate and biomass. This study successfully established a micropropagation technique and Agrobacterium-mediated transformation in duckweed.
Article
Biotechnology & Applied Microbiology
Bert Coleman, Christof Van Poucke, Bavo De Witte, Valentina Casciaro, Tanja Moerdijk-Poortvliet, Koenraad Muylaert, Johan Robbens
Summary: The flavor of T. chuii can be modified by changing the nitrogen supply in the cultivation medium, resulting in differences in odor intensity and taste profile. The N-deplete biomass had a stronger odor intensity and earthy off-odor, while the N-replete biomass had a stronger taste intensity, umami, and salty taste. These findings highlight the importance of cultivating conditions in shaping the flavor and palatability of microalgae biomass.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Review
Plant Sciences
Aparajita Choudhury, Manchikatla Rajam
Summary: Genetic transformation in legume plants is an important area of agricultural research. Despite some progress in improving crop yields, challenges such as recalcitrance and genotype-dependence still exist. Future research may focus on overcoming these obstacles and further increasing yields.
PLANT CELL REPORTS
(2021)
Article
Plant Sciences
Huimin Ren, Yan Xu, Xiaohong Zhao, Yan Zhang, Jamshaid Hussain, Fuqiang Cui, Guoning Qi, Shenkui Liu
Summary: Casuarina equisetifolia is widely used for agroforestry plantations for various purposes. This study established an efficient regeneration system using stem segment explants, optimizing callus induction and regeneration processes. Genetic transformation protocol using Agrobacterium tumefaciens was also optimized, leading to improved transformation efficiency to obtain transgenic plants in about 4 months. The findings will pave the way for further genetic manipulation and functional genomics of C. equisetifolia.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Xiaonan Li, Haiyan Li, Yuzhu Zhao, Peixuan Zong, Zongxiang Zhan, Zhongyun Piao
Summary: This study developed a regeneration system for Chinese cabbage and established a simple and efficient genetic transformation method. The method can obtain transformants in a relatively short period of time, paving the way for further gene editing and functional studies.
HORTICULTURAL PLANT JOURNAL
(2021)
Article
Forestry
Shaofei Dang, Lifeng Zhang, Suying Han, Liwang Qi
Summary: A simple and efficient protocol for the genetic transformation of Japanese larch was developed and multiple transgenic cell lines were obtained. However, the expression levels of exogenous genes in the transgenic system were relatively low, possibly due to the large genome of Japanese larch.
Article
Plant Sciences
Shengnan Liu, Yunlu Shi, Fang Liu, Yan Guo, Minhui Lu
Summary: This study presents an optimized transformation system that improves the infection and genetic transformation frequency of immature maize embryos mediated by Agrobacterium tumefaciens. The use of LaCl3 pretreatment enhances the infection frequency, increases the proportion of positive callus, yields more positive regenerated plantlets, and significantly increases the transformation frequency from 8.40% to 17.60%.
PLANT CELL REPORTS
(2022)
Article
Horticulture
Junxia Zhang, Jieping Wu, Xinyi Hao, Yaping Xie, Kai Lv, Weirong Xu
Summary: This study established a stable and reproducible transformation protocol for grapevine, using immature zygotic embryos. The optimized conditions led to a considerable reduction in the time required for transformation and the method was found to be easily accessible and straightforward to implement.
SCIENTIA HORTICULTURAE
(2023)
Article
Plant Sciences
Xiaoping Wang, Shanshan Chen, Haonan Zhang, Ping Luo, Fangping Zhou, Bingshan Zeng, Jianmin Xu, Chunjie Fan
Summary: An efficient adventitious bud regeneration and transformation system of Eucalyptus was established using the genotype E. urophylla x E. grandis DH32-29, providing a powerful tool for genetic improvement and gene function analysis in Eucalyptus.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agricultural Engineering
Bin Zhang, Xiaomin Zhao, Leyi Zhang, Hongkai Liu, Chuanshu Zhu, Zhiqing Ma
Summary: This study established a transformation system for Tripterygium wilfordii through optimizing the concentrations and combinations of growth regulators. Genetic transformation of T. wilfordii was achieved using Agrobacterium tumefaciens and overexpression of the miltiradiene synthase gene (TwMS) resulted in increased triptolide content. This efficient genetic transformation provides a useful tool for future genetic engineering studies on T. wilfordii.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Plant Sciences
Michelle M. M. Dominguez, Carmen S. S. Padilla, Kranthi K. K. Mandadi
Summary: Developing an efficient transformation system is crucial for genetically engineering recalcitrant crops, especially trees. In this study, we present a stable plant transformation method for citrus genetic engineering using Agrobacterium tumefaciens. The methodology has been optimized for fourteen citrus varieties and includes infection, co-cultivation, selection, and culture media conditions. The transformation efficiency can reach 11%, and transgenic plants can be obtained in as early as six months, depending on the variety. The incorporation of helper virulence genes and antioxidants improves the transformation efficiency of specific recalcitrant cultivars.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Su-Hyeon Moon, Seong-Jun Cho
Summary: In this study, the antioxidant activity of Tetraselmis chuii during simulated gastrointestinal digestion was evaluated using the INFOGEST 2.0 method. The results showed that the antioxidant activity of T. chuii increased during digestion, and the intestinal digesta had significantly higher values of various antioxidant parameters. The study also identified fractions of T. chuii digesta that exhibited significant antioxidant activity. These findings suggest the potential use of T. chuii and its digestive enzyme products as antioxidant agents in functional foods.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Food Science & Technology
Waqas Muhammad Qazi, Simon Ballance, Anne Kjersti Uhlen, Katerina Kousoulaki, John-Erik Haugen, Anne Rieder
Summary: The study revealed that treatment of algae with ethanol can effectively remove pigmentation and volatiles, thereby reducing negative impacts on dough rheology and bread quality. This treatment also leads to improvements in dough stability, resistance to extension, and elastic recovery, as well as an increase in specific volume, decrease in crumb firmness, and increase in slice brightness in bread products. Ethanol treatment of algae could be a feasible strategy to overcome sensory and structural challenges in incorporating algae into foods for potential nutritional benefits.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Plant Sciences
Luca Capriotti, Angela Ricci, Barbara Molesini, Bruno Mezzetti, Tiziana Pandolfini, Irene Piunti, Silvia Sabbadini
Summary: Plant genetic transformation is a powerful breeding tool for improving disease tolerance, stress resistance, fruit production, and quality in fruit trees. This study validated the use of cotyledons and hypocotyls derived from somatic embryos as starting explants for in vitro regeneration and transformation trials in grapevines. The optimized protocols resulted in successful generation of transgenic plants.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
Tanika Thakur, Kshitija Sinha, Tushpinder Kaur, Ritu Kapoor, Gulshan Kumar, Rupam Kumar Bhunia, Prafull Salvi
Summary: Rice is a crucial staple food crop, and an optimized rice transformation protocol is important for studying gene functions and improving rice crops.
Article
Plant Sciences
Susana Manzano, Zoraida Megias, Cecilia Martinez, Alicia Garcia, Encarnacion Aguado, Tarik Chileh, Diego Lopez-Alonso, Federico Garcia-Maroto, Eduard Kejnovsky, Jiri Siroky, Zdenek Kubat, Tereza Kralova, Boris Vyskot, Manuel Jamilena
Article
Biotechnology & Applied Microbiology
Patricia Uebeda-Minguez, Federico Garcia-Maroto, Diego Lopez Alonso
JOURNAL OF APPLIED PHYCOLOGY
(2017)
Review
Chemistry, Analytical
Jose Antonio Garrido-Cardenas, Federico Garcia-Maroto, Jose Antonio Alvarez-Bermejo, Francisco Manzano-Agugliaro
Article
Chemistry, Inorganic & Nuclear
Paul Servin, Regis Laurent, Mar Tristany, Antonio Romerosa, Maurizio Peruzzini, Federico Garcia-Maroto, Jean-Pierre Majoral, Anne-Marie Caminade
INORGANICA CHIMICA ACTA
(2018)
Article
Biochemistry & Molecular Biology
Ana Molina-Marquez, Marta Vila, Rocio Rengel, Emilio Fernandez, Federico Garcia-Maroto, Javier Vigara, Rosa Leon
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Marine & Freshwater Biology
Nahla Bouzidi, Fatma Zili, Federico Garcia-Maroto, Diego Lopez Alonso, Hatem Ben Ouada
JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM
(2020)
Article
Genetics & Heredity
Lilia Gonzalez-Ceron, Jose Cebrian-Carmona, Concepcion M. Mesa-Valle, Federico Garcia-Maroto, Frida Santillan-Valenzuela, Jose Antonio Garrido-Cardenas
Summary: Analysis of genetic and amino acid diversity of pvcyrpa in Mexican and global parasites revealed nine private haplotypes in Southern Mexican sequences. These parasites showed high LD-R-2 index and low nucleotide diversity compared to isolates from South America or Asia. Diversity at pvcyrpa exon-1 is influenced by mutation and recombination, which is likely maintained by balancing selection due to selective immune pressure. Further study on the role of pvcyrpa in immune response and vaccine development is warranted.
Article
Food Science & Technology
Tarik Chileh Chelh, Svetlana Lyashenko, Abdallah Lahlou, El-Hassan Belarbi, Miguel Angel Rincon-Cervera, Ignacio Rodriguez-Garcia, Miguel Urrestarazu-Gavilan, Rosalia Lopez-Ruiz, Jose Luis Guil-Guerrero
Summary: Ahiflower oil, extracted from Buglossoides arvensis seeds, contains high levels of stearidonic acid (SDA, 18:4n-3). In this study, the phenolic composition of Ahiflower oil and several Buglossoides taxa were analyzed, and their in vitro antiproliferative activities against colorectal cancer cells were investigated. The highest amounts of phenolic compounds were found in samples collected from Spain, under a warm Mediterranean climate. Rosmarinic and lithospermic acids were identified as the main phenols in Buglossoides seeds. The seed extracts showed dose and time-dependent inhibitory effects on HT-29 cancer cells, although their anti proliferative activity was lower than that of pure phenolics.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Abdallah Lahlou, Svetlana Lyashenko, Tarik Chileh-Chelh, El-Hassan Belarbi, Irene Torres-Garcia, Miriam Alvarez-Corral, Ignacio Rodriguez-Garcia, Miguel Angel Rincon-Cervera, Jose L. Guil-Guerrero
Summary: Twenty-one Pinus taxa were evaluated to find A5-unsaturated polymethylene-interrupted fatty acids (A5-UPIFA)-rich oils from Pinus seeds. The fatty acid (FA) composition was determined by GC-FID and GC-MS, and NMR of crude oils proved to be a fast method to determine the ratio between A5-UPIFA and total FA. The results show that several underutilized Pinus taxa could be developed into renewable woody oil species, and their seeds could be used as raw materials for A5-UPIFA-rich oils.
Article
Food Science & Technology
Abdallah Lahlou, Tarik Chileh-Chelh, Svetlana Lyashenko, Miguel Angel Rincon-Cervera, Ignacio Rodriguez-Garcia, Rosalia Lopez-Ruiz, Miguel Urrestarazu, Jose Luis Guil-Guerrero
Summary: This study investigated the chemical composition and anti-tumor activity of Arecaceae fruits. The results showed that these fruits are rich in fatty acids, phenolic compounds, and flavonoids. The fruit extracts exhibited inhibitory effects on colorectal cancer cells, indicating the potential functional food value of these Arecaceae fruits.
Article
Plant Sciences
Miguel Angel Rincon-Cervera, Abdallah Lahlou, Tarik Chileh-Chelh, Svetlana Lyashenko, Rosalia Lopez-Ruiz, Jose Luis Guil-Guerrero
Summary: This study analyzed the seeds of 24 Arecaceae species and found that they are rich in fatty acids and phenolic compounds. Some species' seeds can be used as raw materials for healthy oilseed production and have anti-tumor activity. The results suggest that Arecaceae seeds are a cheap source of health-promoting compounds.
Article
Food Science & Technology
Svetlana Lyashenko, Tarik Chileh-Chelh, Miguel Angel Rincon-Cervera, Svetlana P. P. Lyashenko, Zalina Ishenko, Oleg Denisenko, Valentina Karpenko, Irene Torres-Garcia, Jose Luis Guil-Guerrero
Summary: An analysis of the fatty acids, tocols, and squalene in the fillets and roes of 29 species of dry-salted fishes consumed in Eurasian countries revealed their health benefits. Most fish samples had high concentrations of polyunsaturated fatty acids, specifically DHA, EPA, and ARA. The nutritional quality of fish lipids, particularly the n-6/n-3 PUFA ratio, was favorable in all samples. Alpha-tocopherol was found in all fillets and roes, with the highest concentration in the roes of Abramis brama. Dry-salted fish are notable for their high concentrations of ARA, EPA, and DHA, as well as alpha-tocopherol in roes.
Article
Biotechnology & Applied Microbiology
Jose A. Garrido-Cardenas, Xiaoyu Han, Diego L. Alonso, Federico Garcia-Maroto
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2019)
