Article
Plant Sciences
Margaret Mukami Gitau, Attila Farkas, Benedikta Balla, Vince Ordog, Zoltan Futo, Gergely Maroti
Summary: This study investigated the plant-growth-promoting effects of three selected strains of eukaryotic green microalgae on Medicago truncatula plants. The results showed that the application of live algae cells significantly increased plant growth parameters, with Chlorella species demonstrating the most pronounced effects.
Article
Plant Sciences
Beth Szyszka-Mroz, Alexander G. Ivanov, Charles G. Trick, Norman P. A. Huner
Summary: The two cell types of the Antarctic psychrophile, Chlamydomonas priscuii, show differences in photosynthetic performance, with palmelloids having lower abundance of LHCII proteins and higher Chl a/b ratio compared to single cells. Palmelloids also exhibit lower excitation pressure but higher yield of PSII and electron transport rates than single cells, indicating decreased sensitivity to high light.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agricultural Engineering
Victor Pozzobon, Franco Otaola, Clarisse Arnoudts, Jules Lagirarde
Summary: 3D printing is a crucial technology in designing photobioreactors, allowing for rapid prototyping of complex geometries at an affordable cost. This study investigated the biocompatibility of different 3D printing materials with microalgae, revealing that acrylate methacrylate resin inhibits growth and causes oxidative stress, while ABS, PC-Blend, and PLA show nominal performance. PLA is the only material that does not induce an early onset of oxidative stress.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Benan Inan, Basak Akin, Ilayda Dilara Unlue, Anil Tevfik Kocer, Arzu celik, Meyrem Vehapi, Yilmaz Kaya, Didem Ozcimen
Summary: The aim of this study was to isolate and identify Antarctic microalgae and examine the growth responses and biochemical contents of Chlorella variabilis under different cultivation conditions. The specific growth rate, as well as the carbohydrate, protein, chlorophyll-a, and beta-carotene contents of the microalgae, varied with temperature, nitrogen concentration, and light cycle. This study highlights the efficient production of C. variabilis under temperate conditions by manipulating the growth parameters.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Agricultural Engineering
Shabnam Mirizadeh, Alessandro Alberto Casazza, Attilio Converti, Mohsen Nosrati, Seyed Abbas Shojaosadati
Summary: The study aimed to investigate non-destructive lipid extraction from Chlorella vulgaris under stress conditions, using dodecane as the most suitable solvent for the highest lipid yield. The results showed that stressed cells had 1.75 times higher lipid concentration compared to unstressed cells, demonstrating the effectiveness of applying stress conditions and repetitive extractions for lipid recovery.
BIORESOURCE TECHNOLOGY
(2021)
Article
Plant Sciences
Rebecca J. Duncan, Daniel A. Nielsen, Cristin E. Sheehan, Stacy Deppeler, Alyce M. Hancock, Kai G. Schulz, Andrew T. Davidson, Katherina Petrou
Summary: Primary production in the Southern Ocean is dominated by diatom-rich phytoplankton assemblages. However, limited knowledge exists on how diatoms allocate cellular energy in response to ocean acidification (OA). In this study, macromolecular content of selected diatom taxa from an Antarctic phytoplankton community exposed to different fCO(2) levels was analyzed. Species-specific differences in macromolecular partitioning under OA were observed. These changes may have cascading effects on food web dynamics in the Antarctic marine ecosystem.
Article
Environmental Sciences
Antonio Leon-Vaz, Luis C. Romero, Cecilia Gotor, Rosa Leon, Javier Vigara
Summary: This study investigated the effect of cadmium on the microalga Chlorella sorokiniana through a proteomic approach, revealing adjustments in various metabolic pathways and an increase in pathways such as photorespiration and antioxidant enzymes. Additionally, it was found that Chlorella sorokiniana exhibits high tolerance to cadmium exposure, suggesting its potential use in bioremediation processes.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Plant Sciences
Zeno Guardini, Luca Dall'Osto, Simone Barera, Mehrdad Jaberi, Stefano Cazzaniga, Nicola Vitulo, Roberto Bassi
Summary: By applying chemical mutagenesis and selecting for norflurazon-resistant strains, enhanced carotenoid pool size and growth tolerance under photooxidative stress were observed in Chlorella vulgaris. Optimizing carotenoid/chlorophyll ratio and resistance to photooxidative stress improved light-to-biomass conversion efficiency under high light conditions of photobioreactors. Enhanced tolerance to singlet oxygen in Chlorella is promoted by at least two independent mechanisms, only one of which is carotenoid-dependent.
Article
Agricultural Engineering
Lei Yang, Ashiwin Vadiveloo, Ai-Jie Chen, Dong-Zhi Chen, Feng Gao
Summary: In this study, the use of phytohormone gibberellins (GAs) was found to enhance sulfamethoxazole (SMX) removal and lipid accumulation in Chlorella vulgaris microalgae. The addition of GAs increased the SMX removal rate and lipid productivity compared to the control group. GAs promoted stress tolerance and lipid accumulation by up-regulating antioxidase-related genes and genes related to the carbon cycle of microalgal cells. This study highlights the potential of using exogenous GAs to improve the economic benefits of microalgae-based antibiotics removal and biofuel production.
BIORESOURCE TECHNOLOGY
(2023)
Article
Plant Sciences
Michela Cecchin, Jovan Simicevic, Louise Chaput, Manuel Hernandez Gil, Laura Girolomoni, Stefano Cazzaniga, Claire Remacle, Julia Hoeng, Nikolai Ivanov, Bjoern Titz, Matteo Ballottari
Summary: The study investigates the acclimation of the green alga Chlorella vulgaris to different light intensities, focusing on changes in photosynthetic and mitochondrial activities and differentially expressed proteins. The results reveal that in high light conditions, the alga increases mitochondrial respiration to dissipate excess reducing power and regulates various proteins involved in cell metabolism, gene expression, and signaling. These findings have important implications for the industrial application of Chlorella vulgaris.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Nur Diyanah Zamree, Nurul Aini Puasa, Zheng Syuen Lim, Chiew-Yen Wong, Noor Azmi Shaharuddin, Nur Nadhirah Zakaria, Faradina Merican, Peter Convey, Syahida Ahmad, Hasrizal Shaari, Alyza Azzura Azmi, Siti Aqlima Ahmad, Azham Zulkharnain
Summary: Research has shown that Antarctic microorganisms, such as bacteria, yeasts, and fungi, can be used for bioremediation of diesel, offering alternative approaches. However, little attention has been given to using Antarctic microalgae for hydrocarbon degradation. This study focuses on utilizing an Antarctic microalga for the bioremediation of diesel. The microalgal strain used was obtained from Paradise Bay in the western Antarctic Peninsula. The study found that the microalgal strain exhibited higher growth rates under aeration. Factors such as pH, salt concentration, and nitrogen source were optimized for optimal growth and diesel degradation.
Article
Agricultural Engineering
Chengfeng Xue, I. -Son Ng
Summary: By optimizing the process with in situ CO2 adaptation and citrate feeding strategies, biorefineries have successfully achieved efficient production of GABA and sequestered the CO2 generated in sodium hydroxide. Supplying GABA to the culture further improved biomass and lipid content.
BIORESOURCE TECHNOLOGY
(2022)
Article
Engineering, Chemical
Jia Xin Yap, C. P. Leo, Derek Juinn Chieh Chan, Nazlina Haiza Mohd Yasin, Mohd Shaiful Sajab
Summary: Water pollution requires sustainable materials for green treatment solutions. This study successfully extracted nanocellulose from microalgae through alkaline treatment, bleaching, and hydrolysis without TEMPO-mediated oxidation. The effects of acid concentration on nanocellulose properties were studied and the maximum yield was achieved using sulphuric acid with a concentration of 60%(v/v). The extracted microalgae nanocellulose was incorporated into carboxymethyl cellulose/sodium alginate beads, significantly increasing methylene blue adsorption capacity and achieving a high dye removal percentage.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Agricultural Engineering
Shuang Wang, Jiangyi Zhou, Yifei Zhang, Sirong He, Sivakumar Esakkimuthu, Kai Zhu, Sunel Kumar, Gaojin Lv, Xun Hu
Summary: This study demonstrates the importance of using biochar and Chlorella protothecoides together for adsorbing tetracycline in environmental purification. The addition of biochar increases the biomass of Chlorella protothecoides and it proves that Chlorella protothecoides can completely absorb low concentrations of tetracycline.
BIORESOURCE TECHNOLOGY
(2023)
Article
Plant Sciences
Snezana Vojvodic, Milena Dimitrijevic, Milan Zizic, Tanja Ducic, Giuliana Aquilanti, Marina Stanic, Bernd Zechmann, Jelena Danilovic Lukovic, Dalibor Stankovic, Milos Opacic, Arian Morina, Jon K. Pittman, Ivan Spasojevic
Summary: This paper reports a three-step mechanism that the green microalga Chlorella sorokiniana activates during the acquisition of and adaptation to manganese (Mn), which is both an essential trace metal and a pollutant of waters.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Multidisciplinary Sciences
Chiew-Yen Wong, Ming-Li Teoh, Siew-Moi Phang, Phaik-Eem Lim, John Beardall
Review
Chemistry, Medicinal
Chu Wan-Loy, Phang Siew-Moi
Article
Microbiology
David A. Pearce, Irina A. Alekhina, Aleks Terauds, Annick Wilmotte, Antonio Quesada, Arwyn Edwards, Aurelien Dommergue, Birgit Sattler, Byron J. Adams, Catarina Magalhaes, Wan-Loy Chu, Maggie C. Y. Lau, Craig Cary, David J. Smith, Diana H. Wall, Gabriela Eguren, Gwynneth Matcher, James A. Bradley, Jean-Pierre de Vera, Josef Elster, Kevin A. Hughes, Lewis Cuthbertson, Liane G. Benning, Nina Gunde-Cimerman, Peter Convey, Soon Gyu Hong, Steve B. Pointing, Vivian H. Pellizari, Warwick F. Vincent
FRONTIERS IN MICROBIOLOGY
(2016)
Article
Biology
Yih Yih Kok, Wan Loy Chu, Siew Moi Phang, Shar Mohamed Mariam, Naidu Rakesh, Pey Jiun Lai, Shui Nyuk Ling, Joon Wah Mak, Patricia Kim Chooi Lim, Balraj Pauline, Alan Soo Beng Khoo
ARCHIVES OF BIOLOGICAL SCIENCES
(2014)
Article
Plant Sciences
Wan-Loy Chu, Shao-Yang Tneh, Stephen Ambu
Article
Biotechnology & Applied Microbiology
Emienour-Muzalina Mustafa, Siew-Moi Phang, Wan-Loy Chu
JOURNAL OF APPLIED PHYCOLOGY
(2012)
Article
Biotechnology & Applied Microbiology
Yee Voon Tang, Siew Moi Phang, Wan Loy Chu, Anthony Ho, Soo Hwang Teo, Hong Boon Lee
JOURNAL OF APPLIED PHYCOLOGY
(2012)
Article
Biotechnology & Applied Microbiology
Haema Thevanayagam, Shar Mariam Mohamed, Wan-Loy Chu
JOURNAL OF APPLIED PHYCOLOGY
(2014)
Article
Biotechnology & Applied Microbiology
Vejeysri Vello, Siew-Moi Phang, Wan-Loy Chu, Nazia Abdul Majid, Phaik-Eem Lim, Soh-Kheang Loh
JOURNAL OF APPLIED PHYCOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Yih-Yih Kok, Wan-Loy Chu, Siew-Moi Phang, Shar Mariam Mohamed, Rakesh Naidu, Pey-Jiun Lai, Shui-Nyuk Ling, Joon-Wah Mak, Patricia Kim-Chooi Lim, Pauline Balraj, Alan Soo-Beng Khoo
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
(2011)
Review
Pharmacology & Pharmacy
Wu-Thong Choo, Ming-Li Teoh, Siew-Moi Phang, Peter Convey, Wei-Hsum Yap, Bey-Hing Goh, John Beardall
FRONTIERS IN PHARMACOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Wei Hsum Yap, Ming Li Teoh, Yin Quan Tang, Bey-Hing Goh
Summary: The integration of virtual laboratory simulations in a biotechnology course at Taylor's University in Malaysia was well-received by students, leading to increased acceptance and confidence in laboratory skills. Students across different study cohorts perceived the virtual simulations to be beneficial for their learning and application of laboratory techniques, even those who had not been exposed to related laboratory sessions found the simulated activities to provide clear explanations and realistic experiences. The structured simulation-based activities with clear instructions were found to significantly improve students' learning outcomes.
BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION
(2021)
Article
Nutrition & Dietetics
Wan-Loy Chu
CURRENT TOPICS IN NUTRACEUTICAL RESEARCH
(2011)