Article
Environmental Sciences
Clement Gauci, Inka Bartsch, Neusa Martins, Daniel Liesner
Summary: This study investigates the effects of temperature on the fertility and growth of the kelp species Laminaria digitata. It finds that there are persistent effects of temperature on gametogenesis and recruitment within the gametophyte generation, and a cold pre-experimental cultivation enhances the growth and thermal tolerance of juvenile sporophytes. This cross-generational effect suggests that priming of early life cycle stages can increase the resilience and productivity of kelps in marine forest restoration and kelp mariculture.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Yuan Shen, Taizo Motomura, Kensuke Ichihara, Yusuke Matsuda, Ko Yoshimura, Chika Kosugi, Chikako Nagasato
Summary: In this study, researchers successfully used CRISPR-Cas9-mediated genome editing to edit the APT gene in Saccharina japonica. The editing efficiency was 8.64% for female gametophytes and 4.46% for male gametophytes. The edited gametophytes were able to produce resistant sporophytes.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Yan-Hui Bi, Zhi Li, Zhi-Gang Zhou
Summary: Saccharina japonica, an economically and ecologically important brown alga, had hindered its karyotype analysis due to the small and similar metaphase chromosomes. However, the combination of DNA-intercalating dye 4 ', 6-Diamidino-2-phenylindole (DAPI) and fluorescence in situ hybridization (FISH) technique had progressively promoted its karyotype analysis in recent years. Various molecular markers, such as rDNAs, sex-linked markers, telomeric repeated sequences, and SSRs, were used in the karyotyping of S. japonica using FISH and other techniques. Additionally, attempts were made to use immunocytochemistry with kinetochore protein for more accurate chromosome analysis.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Plant Sciences
Yan-Hui Bi, Ya-Ming Qiao, Zhen Wang, Zhi-Gang Zhou
Summary: In this study, a putative extracellular carbonic anhydrase gene Sj alpha-CA2 was cloned from Saccharina japonica gametophytes. The enzyme was found to possess CO2 hydration and esterase activities and was located in the periplasmic space of kelp gametophyte cells. Transcription of Sj alpha-CA2 was induced by elevated HCO3- levels, suggesting its potential role in adapting to higher environmental HCO3- levels by dehydrating HCO3- to generate CO2 for uptake by S. japonica gametophytes.
JOURNAL OF PHYCOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Hui-Min Hao, Yan-Hui Bi, Ning-Ning Wei, Pei-Chong Lin, Shou-Hua Mei, Zhi-Gang Zhou
Summary: This study describes the cloning, characterization, and subcellular localization of Sj beta-CA in Saccharina japonica. The recombinant Sj beta-CA exhibited CO2 hydration and dehydration activities, and was located in the periplasmic space of kelp gametophyte cells. Additionally, a positive correlation between gene transcription and exogenous HCO3- utilization was observed.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Ecology
Daniel James Coaten, Hermann Dreki Guls, Halldor Palmar Halldorsson, Margret Porsteinsdottir
Summary: As the global demand for algae products increases, efficient cultivation methods are becoming more important. This study compared the biochemical components of two types of indigenous brown marine macroalgae in Iceland and mapped them to environmental variables. The results showed a strong correlation between sea surface temperature and carbohydrates (positive) and proteins (negative) in both species, suggesting it may be a key driver in macroalgae biochemical production. Other factors such as total phosphorus and nitrate were also identified as possible modulators. Site-specific environmental factors may have a greater influence on algae biochemical variability than genetic familiarity.
REGIONAL STUDIES IN MARINE SCIENCE
(2023)
Article
Environmental Sciences
Pedro A. Ribeiro, Tonje Naess, Geir Dahle, Lars Asplin, Kenneth Meland, Stein Fredriksen, Kjersti Sjotun
Summary: A study examining the genetic structure and gene flow of Saccharina latissima along the Norwegian coast found a clear isolation pattern along the coast, with lower connectivity between South- and North-Norway and between coastal stations and innermost fjord stations. Genetic adaptation was also observed in populations in the Skagerrak area and in the two fjords.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Sundar Ramanan, Gregory L. Rorrer
Summary: This study compared the growth of Laminaria saccharina female gametophyte filamentous cell suspension cultures in a stirred-tank photobioreactor under batch and fed-batch nutrient addition modes, showing that fed-batch addition of all nutrients enhanced biomass productivity by a factor of two over a batch cultivation process.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Plant Sciences
Alexander P. J. Ebbing, Ronald Pierik, Gregory S. Fivash, Nienke C. J. van de Loosdrecht, Tjeerd J. Bouma, Jacco C. Kromkamp, Klaas Timmermans
Summary: Research has shown that delayed gametophytes of kelp can reproduce sexually after prolonged vegetative growth, depending on the interaction between light intensity and temperature. Seasonality can be used as an aquaculture tool to better control the reproduction of delayed gametophytes.
JOURNAL OF PHYCOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Wouter Visch, Ann I. Larsson, Per Aberg, Gunilla B. Toth
Summary: This study examined the effect of different binder types and application treatments on the adhesion of kelp propagules to cultivation ropes, and evaluated their effectiveness by comparing adhesion densities under different flow velocities. The results showed that the effectiveness of binder-assisted seeding of kelp propagules was not affected by flow velocities and was comparable to the non-binder treatment.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Hiroyuki Mizuta, Toshiki Uji, Hajime Yasui
Summary: The presence of extracellular iodoperoxidase (IPO) can activate the uptake of silicate-Si during an oxidative burst, and Si deposition is promoted in the presence of reactive oxygen species and phlorotannin. These findings suggest a mechanism where defense responses in kelp are facilitated by extracellular silicate-Si uptake.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Biotechnology & Applied Microbiology
Jillian Campbell, Samuel Starko
Summary: This study aimed to establish allometric relationships for the widespread kelp Saccharina latissima in the Salish Sea, comparing the abilities of four non-destructive metrics to predict thallus fresh weight and ranking models based on their AICc scores. The appropriate sample size for constructing local models was determined through a resampling experiment, providing inexpensive and effective methods for non-destructively estimating S. latissima biomass.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Dylan L. C. de Jong, Klaas R. Timmermans, Jose M. de Winter, Goverdina C. H. Derksen
Summary: The study revealed that seaweed phytosterol content was not affected by nutrient availability alone for the first five weeks, but showed significant differences between nutrient treatments in weeks 3-5. In the sixth week, varying light conditions and nutrient availability resulted in marked differences in sterol content among all groups.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Environmental Sciences
Xianming Tang, Linsong Shen, Shangshu Liu, Jiangtao Gao
Summary: Glyphosate is widely used for weed control due to its herbicidal properties and cost-effectiveness, but its numerous products are now recognized as water pollutants. Research shows that the brown alga Saccharina japonica can break down glyphosate to orthophosphate, offering potential for bioremediation of contaminated seawater.
Article
Biotechnology & Applied Microbiology
Louise Kregting, Emma Healey, Molly Crowe, Eoghan M. Cunningham
Summary: Mariculture grown seaweed production is insufficient to meet the demand for traditional and novel applications. Water motion, rather than static conditions, has been found to enhance seaweed growth in aquaculture. This study investigated the influence of water motion on the growth of Saccharina latissima in the nursery stage and found that oscillatory water motion increased overall sporophyte density, while static conditions increased sporophyte length. The results suggest that water motion during the nursery phase can increase the yield of sea-grown kelp in mariculture.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)